BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,927,292	T→C	D46D (GAT→GAC)	asnA →	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,927,292	0	T	C	98.0%	1502.1 / 6.2	414	D46D (GAT→GAC)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (187/213); total (197/217)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 414 total aligned reads displayed.

GAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCA  >  NC_000913/3927144‑3927438

 aGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAAc                                                                                                                                                 >  1:139208/1‑151 (MQ=255)

   tataAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCGGATTCTTAGCCGTGTGGGGGATGGCACGCAGGCCAACtt                                                                                                                                               >  6:756077/1‑151 (MQ=255)

    ataAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTg                                                                                                                                              <  5:1184421/151‑1 (MQ=255)

    ataAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTg                                                                                                                                              <  7:796662/151‑1 (MQ=255)

       aaaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCg                                                                                                                                           <  1:1339604/151‑1 (MQ=255)

        aaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCTCTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCgg                                                                                                                                          >  4:377471/1‑151 (MQ=255)

        aaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCgg                                                                                                                                          <  5:1318334/151‑1 (MQ=255)

        aaaaTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCgg                                                                                                                                          <  6:1190850/151‑1 (MQ=255)

         aaaTGAAAACCGCATACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCggg                                                                                                                                         <  4:750773/151‑1 (MQ=255)

           aTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCt                                                                                                                                       <  4:799918/151‑1 (MQ=255)

           aTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCt                                                                                                                                       <  2:155506/151‑1 (MQ=255)

           aTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTCTGGGGGATTGCACGCAGGACAACTTGTCGGGCt                                                                                                                                       >  5:267988/1‑151 (MQ=255)

             gAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCtgt                                                                                                                                     <  6:1096788/151‑1 (MQ=255)

             gAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCtgg                                                                                                                                     >  3:753264/1‑150 (MQ=255)

              aaaaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCtgtg                                                                                                                                    <  1:224248/151‑1 (MQ=255)

              aaaaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCtgtg                                                                                                                                    >  8:1520609/1‑151 (MQ=255)

               aaaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCtgtg                                                                                                                                    >  1:632301/1‑150 (MQ=255)

               aaaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGa                                                                                                                                   <  7:594602/151‑1 (MQ=255)

                aaCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGa                                                                                                                                   >  4:1054472/1‑150 (MQ=255)

                 aCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaa                                                                                                                                 <  7:141940/151‑1 (MQ=255)

                 aCCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaa                                                                                                                                  >  7:869967/1‑150 (MQ=255)

                  ccGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGAGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaa                                                                                                                                 >  2:38797/1‑150 (MQ=255)

                  ccGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGGCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaaa                                                                                                                                <  2:151691/151‑1 (MQ=255)

                   cGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaaaa                                                                                                                               <  8:485432/151‑1 (MQ=255)

                    gCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAGTCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGaaaaac                                                                                                                              >  7:471621/1‑150 (MQ=255)

                      ttACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCg                                                                                                                            >  8:770658/1‑151 (MQ=255)

                       tACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGc                                                                                                                             <  2:1689254/149‑1 (MQ=255)

                           ttGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGc                                                                                                                        >  3:846596/1‑150 (MQ=255)

                            tGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCa                                                                                                                       <  3:19226/150‑1 (MQ=255)

                               cAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTGGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTa                                                                                                                   <  7:1203261/151‑1 (MQ=255)

                                aaaCAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTa                                                                                                                   >  2:64824/1‑150 (MQ=255)

                                       gTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGaa                                                                                                           <  5:375913/151‑1 (MQ=255)

                                       gTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGa                                                                                                            >  5:1546684/1‑150 (MQ=255)

                                        tCAAATTAGCTTCGTGAAATCTCACTTTTCTCTTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCGGATTCTTAGCCGTGTCGGGGATGGAAAGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGAAGGTAAAAGTGaa                                                                                                           >  5:502659/1‑150 (MQ=255)

                                         cAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAg                                                                                                         <  3:1432808/151‑1 (MQ=255)

                                         cAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAg                                                                                                         <  8:1445540/151‑1 (MQ=255)

                                          aaaTTAGCTTCGTGAAATCTCACTTTTTTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGc                                                                                                        >  5:849753/1‑151 (MQ=255)

                                           aaatataatCGAGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTCGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGc                                                                                                        >  4:971720/9‑150 (MQ=255)

                                           aaTTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGct                                                                                                       >  7:1495082/1‑151 (MQ=255)

                                           aaTTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGct                                                                                                       >  3:1460736/1‑151 (MQ=255)

                                               aGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTg                                                                                                    <  8:393311/150‑1 (MQ=255)

                                               aGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGGCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGc                                                                                                   <  8:22317/151‑1 (MQ=255)

                                                    cGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTTAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCtta                                                                                               <  1:1027218/150‑3 (MQ=255)

                                                    cGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAACGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGAt                                                                                              <  3:42549/151‑1 (MQ=255)

                                                     gTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGCCAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGATAGCTCTGCCTGATg                                                                                             >  1:343901/1‑151 (MQ=255)

                                                         aaTCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTTAAAGTGAAAGCTCTGCCTGATGCCCa                                                                                         <  2:960720/151‑1 (MQ=255)

                                                         aaTCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGccc                                                                                          >  6:178111/1‑150 (MQ=255)

                                                         aaTCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGccc                                                                                          >  3:1303352/1‑150 (MQ=255)

                                                         aaTCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCa                                                                                         <  3:28523/151‑1 (MQ=255)

                                                               aCTTTTCTCGTCAACTGGAAGAACGGCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGa                                                                                   <  2:1173401/151‑1 (MQ=255)

                                                                 ttttCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTCTGAAAAAGCGGTGCAGCTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGa                                                                                   >  2:1433175/1‑149 (MQ=255)

                                                                    tctcGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTg                                                                               <  2:715237/150‑1 (MQ=255)

                                                                       cGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGt                                                                             <  6:1465471/149‑1 (MQ=255)

                                                                          cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcatt                                                                        <  8:648179/151‑1 (MQ=255)

                                                                          cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcatt                                                                        >  3:1593696/1‑151 (MQ=255)

                                                                          cAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcat                                                                         <  5:443220/150‑1 (MQ=255)

                                                                            aCTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGttcattc                                                                       >  7:1138673/1‑150 (MQ=255)

                                                                               ggAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTg                                                                   >  3:1125408/1‑151 (MQ=255)

                                                                               ggAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTg                                                                   >  4:620028/1‑151 (MQ=255)

                                                                               ggAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTg                                                                   <  4:279995/151‑1 (MQ=255)

                                                                                gaagaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTgg                                                                  <  1:1551374/151‑1 (MQ=255)

                                                                                 aagaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACACCTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGc                                                                 >  8:1032925/1‑151 (MQ=255)

                                                                                 aagaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTggg                                                                 <  6:1003267/151‑2 (MQ=255)

                                                                                   gaaCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGa                                                               <  2:1231492/151‑1 (MQ=255)

                                                                                     aCGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAg                                                             <  6:906441/151‑1 (MQ=255)

                                                                                         cTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTgg                                                          >  1:110392/1‑150 (MQ=255)

                                                                                           ggtgCTGATCGAAGTCCAGGCGCCTATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCGGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGaa                                                        <  1:970051/147‑1 (MQ=255)

                                                                                           ggggCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGa                                                         <  4:1572616/149‑1 (MQ=255)

                                                                                           ggggCTGAGCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTgg                                                          <  4:542460/148‑1 (MQ=255)

                                                                                                 gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAg                                                 <  5:1451051/151‑1 (MQ=255)

                                                                                                 gATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAg                                                 <  4:1326785/151‑1 (MQ=255)

                                                                                                  aTCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTc                                                   >  1:430077/1‑148 (MQ=255)

                                                                                                    cGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGCAACGTCAGAcc                                              >  8:1240622/1‑151 (MQ=255)

                                                                                                    cGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGAc                                               <  6:653183/150‑1 (MQ=255)

                                                                                                         tacAGGCGCCCATTCTTAGCCGAGTGGGGTATGGCACTCATTACAAATTGTCCGGCTGTTAAAAAGCGCTGCAGGTAAAAGTGAAAGCTCTGCCTGATGACCAGTTAGAAGTGGATCATTCAGATGCGAACTGGATAAGTCAGACCTTAgg                                         >  1:915038/3‑151 (MQ=255)

                                                                                                            aGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCa                                      <  6:1316740/151‑1 (MQ=255)

                                                                                                               cgcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAac                                    >  2:922045/1‑150 (MQ=255)

                                                                                                                gcCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAacac                                  >  2:235883/1‑151 (MQ=255)

                                                                                                                 ccGATTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACg                                 >  7:1572629/1‑151 (MQ=255)

                                                                                                                    aTTCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACt                              <  4:1026427/151‑1 (MQ=255)

                                                                                                                      tCTTAGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTc                            >  2:1381270/1‑151 (MQ=255)

                                                                                                                         tAGCGGTGGGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTc                            <  7:445460/148‑1 (MQ=255)

                                                                                                                          aGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAgcg                        <  8:1598127/151‑1 (MQ=255)

                                                                                                                          aGCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAgc                         <  4:665559/150‑1 (MQ=255)

                                                                                                                          aGCCGTGTGGGGGATGGCACGCAGGACAACTTGGCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAgc                         <  1:812693/150‑1 (MQ=255)

                                                                                                                           gCCGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAgcgc                       <  2:577223/151‑1 (MQ=255)

                                                                                                                            ccGTGTGGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGcgcg                      <  4:474764/151‑1 (MQ=255)

                                                                                                                             cGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCgg                     >  4:122550/1‑151 (MQ=255)

                                                                                                                                gtgGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGc                   <  6:402664/150‑1 (MQ=255)

                                                                                                                                gtgGGGGATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCg                  <  2:334326/151‑1 (MQ=255)

                                                                                                                                  gggggATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGa                 <  4:874428/150‑1 (MQ=255)

                                                                                                                                   ggggATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAg               <  5:644722/151‑1 (MQ=255)

                                                                                                                                   ggggATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAg               <  3:618947/151‑1 (MQ=255)

                                                                                                                                     ggATGGCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAggg             <  7:741154/151‑1 (MQ=255)

                                                                                                                                          gCACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTa        <  8:743140/151‑1 (MQ=255)

                                                                                                                                           cACGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTac       >  7:627011/1‑151 (MQ=255)

                                                                                                                                            aCGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTac       >  5:17159/1‑150 (MQ=255)

                                                                                                                                             cGCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTTAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTtcac     >  2:1137454/1‑151 (MQ=255)

                                                                                                                                              gCAGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACg    <  6:1609940/151‑1 (MQ=255)

                                                                                                                                                aGGACAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGca  <  6:58959/151‑1 (MQ=255)

GAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGGGGGATGGCACGCAGGATAACTTGTCGGGCTGTGAAAAAGCGGTGCAGGTAAAAGTGAAAGCTCTGCCTGATGCCCAGTTCGAAGTGGTTCATTCACTGGCGAAGTGGAAACGTCAGACCTTAGGGCAACACGACTTCAGCGCGGGCGAAGGGCTGTACACGCA  >  NC_000913/3927144‑3927438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`