Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,099 C→T intergenic (‑130/‑223) yifB ← / → ilvL magnesium chelatase family protein and putative transcriptional regulator/ilvG operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,0990CT97.1% 1490.9 / 16.7 448intergenic (‑130/‑223)yifB/ilvLmagnesium chelatase family protein and putative transcriptional regulator/ilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (4/9);  new base T (194/240);  total (198/250)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 448 total aligned reads displayed.

GAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAA  >  NC_000913/3949951‑3950246
                                                                                                                                                    |                                                                                                                                                   
  aCAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGc                                                                                                                                                 >  5:1265358/1‑151 (MQ=255)
   cAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGc                                                                                                                                                 >  4:1225866/1‑150 (MQ=255)
   cAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCa                                                                                                                                                <  3:1429635/151‑1 (MQ=255)
           aGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCa                                                                                                                                        <  1:542306/151‑1 (MQ=255)
            gTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGc                                                                                                                                         >  6:437063/1‑149 (MQ=255)
              gACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGc                                                                                                                                      <  5:896528/150‑1 (MQ=255)
               aCATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCgg                                                                                                                                    <  7:86048/151‑1 (MQ=255)
                    aGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGc                                                                                                                                         >  7:971233/1‑141 (MQ=255)
                    aGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACaa                                                                                                                                >  5:1375455/1‑150 (MQ=255)
                     gCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACaa                                                                                                                                >  7:197465/1‑149 (MQ=255)
                      cccTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAgg                                                                                                                             >  1:1320731/1‑151 (MQ=255)
                      cccTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATAATGTTCGCAAAAATGCAGCGGACAAAgg                                                                                                                             >  7:551598/1‑151 (MQ=255)
                       cctcctTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGa                                                                                                                            <  7:1257086/151‑1 (MQ=255)
                          cctTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGAt                                                                                                                           <  8:1414513/149‑1 (MQ=255)
                          cctTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTACTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATg                                                                                                                          >  8:1178012/1‑150 (MQ=255)
                            ttGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAAc                                                                                                                       >  5:1064102/1‑151 (MQ=255)
                             tGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAAc                                                                                                                       <  7:771479/150‑1 (MQ=255)
                               aGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTAc                                                                                                                    >  3:413801/1‑151 (MQ=255)
                               aGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTAc                                                                                                                    <  2:292547/151‑1 (MQ=255)
                                   aCCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAgg                                                                                                                <  3:587928/151‑1 (MQ=255)
                                   aCCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAgg                                                                                                                <  8:827406/151‑1 (MQ=255)
                                     cATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGCACTACGAGGa                                                                                                               >  8:206021/1‑150 (MQ=255)
                                     cATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGaa                                                                                                              <  2:1316058/151‑1 (MQ=255)
                                      aTTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGa                                                                                                               >  2:501632/1‑149 (MQ=255)
                                       ttATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATg                                                                                                                          >  6:1118614/1‑137 (MQ=255)
                                         aTGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAggg                                                                                                           >  7:1546120/1‑150 (MQ=255)
                                         aTGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAggg                                                                                                           <  3:290678/150‑1 (MQ=255)
                                         aTGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGa                                                                                                          <  4:851518/151‑1 (MQ=255)
                                          tgtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTTGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGa                                                                                                          <  2:941424/150‑1 (MQ=255)
                                          tgtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGa                                                                                                          >  6:1543411/1‑150 (MQ=255)
                                          tgtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGa                                                                                                          >  5:537907/1‑150 (MQ=255)
                                           gtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaac                                                                                                        >  4:149907/1‑151 (MQ=255)
                                           gtgCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGaac                                                                                                        <  6:1204657/151‑1 (MQ=255)
                                                tAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACAt                                                                                                   >  3:540008/1‑151 (MQ=255)
                                                      atCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATAc                                                                                             >  1:322477/1‑151 (MQ=255)
                                                        cGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTg                                                                                           <  3:1099214/151‑1 (MQ=255)
                                                         gctgctGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTg                                                                                           >  8:1546743/1‑150 (MQ=255)
                                                               gTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGa                                                                                    <  3:1583749/151‑1 (MQ=255)
                                                                 aGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAt                                                                                  >  1:701024/1‑151 (MQ=255)
                                                                   cccGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAttt                                                                                <  5:753097/151‑1 (MQ=255)
                                                                    ccGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAtttt                                                                               >  6:753663/1‑151 (MQ=255)
                                                                      gCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAtttttt                                                                             <  8:717966/151‑1 (MQ=255)
                                                                      gCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAtttttt                                                                             <  1:1593761/151‑1 (MQ=255)
                                                                       cTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAttttttt                                                                            >  1:918701/1‑151 (MQ=255)
                                                                         aaTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAAttttttt                                                                            <  8:110411/149‑1 (MQ=255)
                                                                          aTTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtcac                                                                         >  6:823577/1‑151 (MQ=255)
                                                                           ttCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAATTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTtcact                                                                        >  4:480158/1‑151 (MQ=255)
                                                                               tGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTcactcact                                                                    >  4:692786/1‑151 (MQ=255)
                                                                                gAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTcactcact                                                                    >  8:591519/1‑150 (MQ=255)
                                                                                 atttttAGTGGCTGATTGCTGTTTATTTGTGCAAGGGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtat                                                                  <  4:991932/149‑1 (MQ=255)
                                                                                 aaTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtat                                                                  <  5:339771/151‑1 (MQ=255)
                                                                                  aTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatt                                                                 <  7:1069852/151‑1 (MQ=255)
                                                                                  aTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatt                                                                 <  4:1543426/151‑1 (MQ=255)
                                                                                  aTTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatt                                                                 <  2:827237/151‑1 (MQ=255)
                                                                                    tttAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatttt                                                               >  2:1086833/1‑151 (MQ=255)
                                                                                    tttAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtatttt                                                               >  2:414387/1‑151 (MQ=255)
                                                                                    tttAGTGCCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattt                                                                <  4:111462/150‑1 (MQ=255)
                                                                                       aGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattttatt                                                            <  4:392882/151‑1 (MQ=255)
                                                                                       aGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattttatt                                                            >  2:122592/1‑151 (MQ=255)
                                                                                        gTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACtattttattt                                                           <  2:607146/151‑1 (MQ=255)
                                                                                          ggCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt                                                         <  5:787545/151‑1 (MQ=255)
                                                                                           gCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt                                                         >  3:916047/1‑150 (MQ=255)
                                                                                           gCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTa                                                        <  7:1351905/151‑1 (MQ=255)
                                                                                             tGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTat                                                       >  6:1353091/1‑149 (MQ=255)
                                                                                             tGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaa                                                       >  1:1000203/1‑150 (MQ=255)
                                                                                             tGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaa                                                       >  4:1264786/1‑150 (MQ=255)
                                                                                              gATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaa                                                      <  8:445141/150‑1 (MQ=255)
                                                                                              gATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaa                                                      >  7:1149297/1‑150 (MQ=255)
                                                                                              gATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaa                                                       <  2:731297/149‑1 (MQ=255)
                                                                                               aTTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaa                                                     >  1:851678/1‑150 (MQ=255)
                                                                                               aTTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGAAGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaa                                                    <  5:231928/151‑1 (MQ=255)
                                                                                                 tCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTaaaaaa                                                   <  1:1482846/150‑1 (MQ=255)
                                                                                                    tGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                               >  8:367220/1‑151 (MQ=255)
                                                                                                    tGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAaacaac                                               >  7:1499804/1‑151 (MQ=255)
                                                                                                       ttATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAcaacaa                                             <  3:683808/150‑1 (MQ=255)
                                                                                                       ttATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt                                            >  4:738051/1‑151 (MQ=255)
                                                                                                        tATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAAt                                            <  1:1672751/150‑1 (MQ=255)
                                                                                                           ttGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTta                                         <  6:273155/150‑1 (MQ=255)
                                                                                                             gtgCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTatat                                      >  3:793866/1‑151 (MQ=255)
                                                                                                               gCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTg                                    <  3:1277732/151‑1 (MQ=255)
                                                                                                                cAAGTGAAGTTGAGTTGTTCTGGCGGTTGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                                   <  4:81625/151‑1 (MQ=255)
                                                                                                                 aaGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAGCATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                                   >  1:1153973/1‑150 (MQ=255)
                                                                                                                 aaGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                                  <  4:198792/151‑1 (MQ=255)
                                                                                                                 aaGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGa                                   >  4:1614209/1‑150 (MQ=255)
                                                                                                                 aaGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaa                                  <  5:1556597/151‑1 (MQ=255)
                                                                                                                  aGTGAAGTTGAGTTGTTCTGGCGGTTGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGaaa                                 <  7:922480/151‑1 (MQ=255)
                                                                                                                    tGAAGTTGAGTTGTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAatt                               >  5:457700/1‑151 (MQ=255)
                                                                                                                       agttgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAattatt                            <  8:1350758/151‑1 (MQ=255)
                                                                                                                         ttgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta                           >  1:1232530/1‑150 (MQ=255)
                                                                                                                         ttgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAAttatta                           >  3:1231226/1‑150 (MQ=255)
                                                                                                                          tgagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaa                          >  5:1474912/1‑150 (MQ=255)
                                                                                                                           gagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTaaa                         <  6:924720/150‑1 (MQ=255)
                                                                                                                           gagttgTTCTGGCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAAc                        <  7:1220790/151‑1 (MQ=255)
                                                                                                                                     ggCGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa               <  7:65009/150‑1 (MQ=255)
                                                                                                                                     ggCGGTGGAAAGATGGTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaa               <  5:997931/150‑1 (MQ=255)
                                                                                                                                       cGGTGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaaa            <  8:1533390/151‑1 (MQ=255)
                                                                                                                                          tGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg         >  3:1549415/1‑151 (MQ=255)
                                                                                                                                          tGGAATGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCg         >  7:399002/1‑151 (MQ=255)
                                                                                                                                             aaTGATGTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGcc      <  5:1183031/151‑1 (MQ=255)
                                                                                                                                                 atgTTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa  <  6:592834/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACAATTGACAGTGACATAAGCCCTCCTTGAGTCACCATTATGTGCATAAGATATCGCTGCTGTAGCCCGCTAATTCGTGAATTTTAGTGGCTGATTCCTGTTTATTTGTGCAAGTGAAGTTGAGTTGTTCTGGCGGTGGAATGATGCTCGCAAAAATGCAGCGGACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAA  >  NC_000913/3949951‑3950246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: