Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 290,175 G→A T44I (ACC→ATC)  argF ← ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913290,1750GA97.8% 1378.2 / 10.5 408T44I (ACC→ATC) argFornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base G (4/5);  new base A (195/202);  total (201/207)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 408 total aligned reads displayed.

GTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCG  >  NC_000913/290028‑290322
                                                                                                                                                   |                                                                                                                                                   
 tgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTACCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATa                                                                                                                                                   >  6:265268/1‑149 (MQ=255)
 tgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATa                                                                                                                                                   >  1:1004869/1‑149 (MQ=255)
   tCCTTAATTGACTCTTTATGCCCAATCTGGCTGTCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCCAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCCAAGATGAGCGCGATGTTTTTACCGATAAGCt                                                                                                                                               >  4:1408148/1‑151 (MQ=255)
    ccTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGTTCAAATGCGGCAACTTCGAAATAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCt                                                                                                                                               <  5:1191033/150‑1 (MQ=255)
     cTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCACCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTc                                                                                                                                             >  4:56527/1‑151 (MQ=255)
     cTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTc                                                                                                                                             >  3:1300056/1‑151 (MQ=255)
       tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTg                                                                                                                                           >  6:1682041/1‑151 (MQ=255)
        aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGt                                                                                                                                          <  1:26136/151‑1 (MQ=255)
        aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGt                                                                                                                                          <  7:1667837/151‑1 (MQ=255)
         aTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTa                                                                                                                                         <  7:131656/151‑1 (MQ=255)
             aCTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTc                                                                                                                                     <  8:1698165/151‑1 (MQ=255)
              ctctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTcc                                                                                                                                    >  2:858025/1‑151 (MQ=255)
                  ttATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCt                                                                                                                                   <  8:504895/148‑1 (MQ=255)
                  ttATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTg                                                                                                                                 <  8:402740/150‑1 (MQ=255)
                    aTGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGcc                                                                                                                               >  4:120203/1‑150 (MQ=255)
                    aTGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCa                                                                                                                              <  1:439231/151‑1 (MQ=255)
                    aTGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCa                                                                                                                              <  8:1223508/151‑1 (MQ=255)
                      gCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAt                                                                                                                             <  6:1372728/150‑1 (MQ=255)
                       cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAttt                                                                                                                           <  5:1297725/151‑1 (MQ=255)
                       cccAATCTAGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAttt                                                                                                                           <  6:767735/151‑1 (MQ=255)
                          aaTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAtttttt                                                                                                                        >  1:840409/1‑151 (MQ=255)
                           aTCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAttttttt                                                                                                                       >  2:198900/1‑151 (MQ=255)
                              tGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTAt                                                                                                                    >  3:1141748/1‑151 (MQ=255)
                                  tGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCggg                                                                                                                >  5:860350/1‑150 (MQ=255)
                                    ccGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCtt                                                                                                              <  6:949127/151‑1 (MQ=255)
                                      gCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTg                                                                                                            <  5:1667024/151‑1 (MQ=255)
                                       cTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTg                                                                                                            >  4:756326/1‑150 (MQ=255)
                                       cTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTg                                                                                                            >  3:1354629/1‑150 (MQ=255)
                                        tCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTg                                                                                                            >  7:555581/1‑149 (MQ=255)
                                        gcGGCCCTAAATAGGTAACGCGCGCGCCCTGGGCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAg                                                                                                          <  6:441535/150‑1 (MQ=255)
                                         cGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAg                                                                                                          <  8:1660963/150‑1 (MQ=255)
                                         cGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAg                                                                                                          <  7:1478159/150‑1 (MQ=255)
                                            cccTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTAGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCtgt                                                                                                      >  1:174453/1‑151 (MQ=255)
                                                 aaTAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGc                                                                                                 >  5:543864/1‑151 (MQ=255)
                                                    aGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCaa                                                                                               <  6:1014422/150‑1 (MQ=255)
                                                    aGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCa                                                                                                >  1:690104/1‑149 (MQ=255)
                                                     ggTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAgg                                                                                             >  4:379503/1‑151 (MQ=255)
                                                        aaCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGtg                                                                                          >  2:523281/1‑150 (MQ=255)
                                                           gcgcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGACATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagc                                                                                       >  4:974136/1‑151 (MQ=255)
                                                            cgcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagca                                                                                      <  7:217634/151‑1 (MQ=255)
                                                            cgcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagc                                                                                       >  5:1515023/1‑150 (MQ=255)
                                                             gcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagc                                                                                       >  1:1418916/1‑149 (MQ=255)
                                                              cgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCaga                                                                                    <  3:296821/151‑1 (MQ=255)
                                                              cgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCAATTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagc                                                                                       >  2:1152617/1‑148 (MQ=255)
                                                               gcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagcag                                                                                     >  8:8557/1‑149 (MQ=255)
                                                                cgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTcagcag                                                                                     >  1:1143907/1‑148 (MQ=255)
                                                                cgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGGACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAgaga                                                                                  <  1:1502345/151‑1 (MQ=255)
                                                                   ccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGt                                                                               <  4:1571853/151‑1 (MQ=255)
                                                                         cAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACtg                                                                         >  4:257671/1‑151 (MQ=255)
                                                                           aaTGCGGCAACCTCCAAAGCCCAACGGGGACGACGCGAGTCTTTTTCGAACATGACCGCGTTGTTTTTACCGATAACCCTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACtgtg                                                                       <  6:724583/151‑1 (MQ=255)
                                                                             tGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGc                                                                      >  7:801928/1‑150 (MQ=255)
                                                                               cGGCTACTTCGAAAGAGCAACGTGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCTATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGAGCAgg                                                                   <  4:472338/151‑1 (MQ=255)
                                                                                ggCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAggg                                                                  <  2:1320887/151‑1 (MQ=255)
                                                                                 gCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAgggg                                                                 >  8:1383240/1‑151 (MQ=255)
                                                                                   aaCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTa                                                               <  1:1644301/151‑1 (MQ=255)
                                                                                    aCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaa                                                              <  5:905116/151‑1 (MQ=255)
                                                                                    aCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaa                                                              <  3:514649/151‑1 (MQ=255)
                                                                                     cTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGTCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAATTGAACTGTGCATGGGTaa                                                              >  3:1601902/1‑150 (MQ=255)
                                                                                      ttCGAAAGAGCAACGGGTACGAGTCCACTCCTTTTATAAGATGAGCGCGAAGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGTCTTTTAGCTGTGCGGCAAGGGTCCGGAGAGAAGTGAACTGTGCAGGGGTaa                                                              >  7:1208275/1‑149 (MQ=255)
                                                                                       tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTTGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaaa                                                             <  7:1133455/149‑1 (MQ=255)
                                                                                       tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGt                                                           >  5:4909/1‑151 (MQ=255)
                                                                                        cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTc                                                          <  4:1480948/151‑1 (MQ=255)
                                                                                          aaaGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCg                                                         >  3:684676/1‑150 (MQ=255)
                                                                                           aaGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCg                                                         >  2:744815/1‑149 (MQ=255)
                                                                                           aaGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAg                                                       <  4:430857/151‑1 (MQ=255)
                                                                                            agagCAACGGGTACTAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGc                                                      <  3:1446907/151‑1 (MQ=255)
                                                                                              atcAACGGTTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATTTTTTTACGGATAAGCTTCTGTACTTCCTTGCGATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCATCAGAGAAGTGAACTGTGGAGGTGTAAAGTCGAGCAg                                                    <  1:940171/149‑1 (MQ=38)
                                                                                               gCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGt                                                   <  8:608322/151‑1 (MQ=255)
                                                                                               gCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGt                                                   >  3:938213/1‑151 (MQ=255)
                                                                                                cAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGtt                                                  <  2:1440991/151‑1 (MQ=255)
                                                                                                 aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                 <  6:1299246/151‑1 (MQ=255)
                                                                                                 aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                 >  1:1274097/1‑151 (MQ=255)
                                                                                                 aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                 <  3:855211/151‑1 (MQ=255)
                                                                                                  ccggcgaCGACTCGCCTCTTTGTCGCAGATGCGCGCGATGTGTTTACCGATAAGCTGCTGTACCTCCATGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGGGCAGGGGTAAAGTCGAGCAGTTTc                                                <  4:1046986/145‑1 (MQ=255)
                                                                                                   cGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCa                                               <  6:1196714/151‑1 (MQ=255)
                                                                                                    gggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAACTCGAGCAGTTTc                                                >  8:103443/1‑149 (MQ=255)
                                                                                                     ggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttta                                                >  6:868160/1‑147 (MQ=255)
                                                                                                        aCGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGaa                                            >  4:1594476/1‑149 (MQ=255)
                                                                                                         cgagtcgagtCTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAgt                                         <  1:1593445/151‑1 (MQ=255)
                                                                                                         cgagtcgagtCGTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATAGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACGGGGCAGGGGTAAAGTCGAGCAGTTTCAGAAAgt                                         >  5:599107/1‑151 (MQ=255)
                                                                                                            gtcgagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtt                                      <  5:1449967/151‑1 (MQ=255)
                                                                                                               gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGttttt                                   >  3:1545406/1‑151 (MQ=255)
                                                                                                               gagtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGttttt                                   >  4:111191/1‑151 (MQ=255)
                                                                                                                     ttttCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGta                               <  3:449576/149‑1 (MQ=255)
                                                                                                                     ttttCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGt                                <  5:133614/148‑1 (MQ=255)
                                                                                                                     ttttCGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGt                                <  5:68109/148‑1 (MQ=255)
                                                                                                                         cGAAGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAAt                          <  2:632322/150‑1 (MQ=255)
                                                                                                                           aaGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCgg                       >  6:315147/1‑151 (MQ=255)
                                                                                                                            aGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCgg                       >  7:354801/1‑150 (MQ=255)
                                                                                                                            aGATGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGa                      <  5:396347/151‑1 (MQ=255)
                                                                                                                              aTGAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACa                    <  4:1300270/151‑1 (MQ=255)
                                                                                                                                gAGCGCGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAt                   <  3:1067596/150‑1 (MQ=255)
                                                                                                                                  gcgcGATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAtttt                <  2:310719/151‑1 (MQ=255)
                                                                                                                                    gcgATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTAt              >  4:820284/1‑151 (MQ=255)
                                                                                                                                      gATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATcc            >  5:105745/1‑151 (MQ=255)
                                                                                                                                      gATGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATcc            <  8:732586/151‑1 (MQ=255)
                                                                                                                                        tGTTTTTACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCt           <  7:1402138/150‑1 (MQ=255)
                                                                                                                                           ttttACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGc        <  8:287918/150‑1 (MQ=255)
                                                                                                                                           ttttACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTGGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGc        >  6:1337167/1‑150 (MQ=255)
                                                                                                                                            tttACCGATAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCAt      >  1:530813/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTGTCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCG  >  NC_000913/290028‑290322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑