Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,084,932 G→T F297L (TTC→TTA)  fdoG ← formate dehydrogenase‑O, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,084,9320GT97.5% 1542.4 / 22.8 438F297L (TTC→TTA) fdoGformate dehydrogenase‑O, large subunit
Reads supporting (aligned to +/- strand):  ref base G (4/7);  new base T (197/230);  total (201/237)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 438 total aligned reads displayed.

AGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAAT  >  NC_000913/4084784‑4085078
                                                                                                                                                    |                                                                                                                                                  
  ccGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCTTTGGTATAGGCTTCGGTGTATTCGCGGTTTAAtt                                                                                                                                                <  7:419042/151‑1 (MQ=255)
       ttCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTc                                                                                                                                            <  7:220553/150‑1 (MQ=255)
        tCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCa                                                                                                                                           <  1:1503760/150‑1 (MQ=255)
        tCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCa                                                                                                                                           <  1:459909/150‑1 (MQ=255)
          gTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTg                                                                                                                                        <  6:1438797/151‑1 (MQ=255)
            ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGtt                                                                                                                                      <  3:1079340/151‑1 (MQ=255)
            ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGtt                                                                                                                                      <  2:495242/151‑1 (MQ=255)
            ccAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGtt                                                                                                                                      >  8:729151/1‑151 (MQ=255)
             cAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTc                                                                                                                                     <  4:1279770/151‑1 (MQ=255)
                ttCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcagc                                                                                                                                  >  4:115398/1‑151 (MQ=255)
                ttCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGGTTAATTTTTCAGTGTTcagc                                                                                                                                  >  4:1350340/1‑151 (MQ=255)
                 tCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcagc                                                                                                                                  >  4:231251/1‑150 (MQ=255)
                  cATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcagcag                                                                                                                                <  7:146358/151‑1 (MQ=255)
                  cATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTcagcag                                                                                                                                <  5:731552/151‑1 (MQ=255)
                   aTAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAgg                                                                                                                               >  6:737686/1‑151 (MQ=255)
                     aaGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGt                                                                                                                              <  6:635278/150‑1 (MQ=255)
                     aaGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTa                                                                                                                             <  1:1302146/151‑1 (MQ=255)
                      aGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAc                                                                                                                            <  2:1131492/151‑1 (MQ=255)
                      aGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAc                                                                                                                            >  8:679583/1‑151 (MQ=255)
                       gTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACa                                                                                                                           <  5:163942/151‑1 (MQ=255)
                       gTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACa                                                                                                                           <  5:1076276/151‑1 (MQ=255)
                       gTCCAGGAGGATTTATCGTACTTGCGTTATTCCGCGTCGTAGCCGGTGACCAGGCCATCTTCAAAGCCGTAATCCTCCCGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTGTAATTTTTCAGTGTTCAGACGGTAc                                                                                                                            >  2:877640/1‑150 (MQ=255)
                        tCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAc                                                                                                                            >  4:1080536/1‑149 (MQ=255)
                         ccAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGc                                                                                                                         <  3:1421364/151‑1 (MQ=255)
                              aggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATAc                                                                                                                    <  3:439771/151‑1 (MQ=255)
                              aggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATAc                                                                                                                    >  3:984504/1‑151 (MQ=255)
                              aggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATAc                                                                                                                    >  7:1062124/1‑151 (MQ=255)
                              aggaTTTATCGTACTTGAGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGGAATAc                                                                                                                    <  7:1387335/151‑1 (MQ=255)
                               ggaTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACg                                                                                                                   <  2:1333264/151‑1 (MQ=255)
                                 aTTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGcc                                                                                                                 <  2:357304/151‑1 (MQ=255)
                                  tttATCGTACTTGCGTTTTTCCTCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCAAGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGCTAAAGCAATACCCCt                                                                                                                >  6:879869/1‑151 (MQ=255)
                                   ttATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTg                                                                                                               >  6:1600512/1‑151 (MQ=255)
                                   ttATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTg                                                                                                               >  2:1414654/1‑151 (MQ=255)
                                    tATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGTAGGTACAGCAATACGCCTg                                                                                                               <  3:1525614/150‑1 (MQ=255)
                                     aTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGAc                                                                                                             >  4:675081/1‑151 (MQ=255)
                                     aTCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGAc                                                                                                             >  1:234227/1‑151 (MQ=255)
                                      tCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGAc                                                                                                             >  1:100955/1‑150 (MQ=255)
                                        gTACTTGCGTTTTTCCGCGTCGTAGACGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAgg                                                                                                          <  4:505165/151‑1 (MQ=255)
                                          aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGa                                                                                                         >  6:1129016/1‑150 (MQ=255)
                                          aCTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGa                                                                                                         >  5:1574087/1‑150 (MQ=255)
                                             tGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAg                                                                                                      <  2:1064196/150‑1 (MQ=255)
                                              gCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGc                                                                                                     >  6:1312003/1‑150 (MQ=255)
                                                   tttCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGt                                                                                                <  6:37991/150‑1 (MQ=255)
                                                     tCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCa                                                                                              <  7:57963/150‑1 (MQ=255)
                                                       cgcgTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGt                                                                                            >  4:845629/1‑150 (MQ=255)
                                                           tcgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCATGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCgg                                                                                       >  3:1164474/1‑151 (MQ=255)
                                                             gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGaa                                                                                     <  5:472613/151‑1 (MQ=255)
                                                               aGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACg                                                                                   <  3:733985/151‑1 (MQ=255)
                                                               aGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATACGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACg                                                                                   >  1:1383608/1‑151 (MQ=255)
                                                                        aCAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGc                                                                          <  3:237154/151‑1 (MQ=255)
                                                                        aCAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGc                                                                          >  4:351673/1‑151 (MQ=255)
                                                                             ccATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtagt                                                                     <  8:1170386/151‑1 (MQ=255)
                                                                             ccATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtagt                                                                     <  8:879312/151‑1 (MQ=255)
                                                                              cATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtagt                                                                     >  5:1415330/1‑150 (MQ=255)
                                                                                   tCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTTTTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCAtagtag                                                                   <  7:98013/147‑1 (MQ=255)
                                                                                   tCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTc                                                                 <  7:697859/149‑1 (MQ=255)
                                                                                   tCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTc                                                                 <  2:1681856/149‑1 (MQ=255)
                                                                                   tCAAAGCCGAAATCCTCACGCACGATCAGGCTGCCGTTGGTATAGGCTTCCGTGAATTCGCGGTTTAATTTTTCATAGTTCACCAGGTACAGCAATACCCCTGACAGGAAAGCAAAGACAGTACCGGAACGAATAGGGGCATAGTAGTCa                                                                >  1:615472/1‑150 (MQ=255)
                                                                                    cAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAg                                                               <  8:1311846/150‑1 (MQ=255)
                                                                                      aaGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTa                                                            <  1:1557924/151‑1 (MQ=255)
                                                                                        gCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAcc                                                          <  7:859141/151‑1 (MQ=255)
                                                                                         ccGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccg                                                         <  3:574256/151‑1 (MQ=255)
                                                                                             aaTCCTCACGCACGATCAGGCTGGCGTTGGTATAGAATTCGGTGTATTCGCGGTTTAATTTTTAATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAAAGAATAGGGGCATAGTAGTCAGCTAccgccgc                                                     >  6:479346/1‑151 (MQ=255)
                                                                                              aTCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAccgccgcc                                                    >  2:1477578/1‑151 (MQ=255)
                                                                                                  tCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAc                                                >  2:785102/1‑151 (MQ=255)
                                                                                                   cacgcacgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcg                                               >  7:309782/1‑151 (MQ=255)
                                                                                                   cacgcacgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcg                                               <  1:845455/151‑1 (MQ=255)
                                                                                                     cgcacgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAcgcg                                             >  2:907405/1‑151 (MQ=255)
                                                                                                        acgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTa                                           >  8:393453/1‑150 (MQ=255)
                                                                                                        acgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTa                                           >  1:34557/1‑150 (MQ=255)
                                                                                                         cgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaaa                                         <  3:881984/151‑1 (MQ=255)
                                                                                                         cgATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaaa                                         <  6:219093/151‑1 (MQ=255)
                                                                                                          gATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTaa                                          >  8:460963/1‑149 (MQ=255)
                                                                                                             cAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCg                                      >  8:224164/1‑150 (MQ=255)
                                                                                                              aGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAg                                    <  5:847174/151‑1 (MQ=255)
                                                                                                                  tGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatc                                >  3:63303/1‑151 (MQ=255)
                                                                                                                   ggCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAGAGCAATACGCCTGACAGGAAAGCAATGGCAGTACCGGAACGAATAGGGGGATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgat                                 <  2:1183787/149‑1 (MQ=255)
                                                                                                                   ggCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatcg                               <  8:1049588/151‑1 (MQ=255)
                                                                                                                    gCGTTGGTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGgatcga                              <  8:1503040/151‑1 (MQ=255)
                                                                                                                         ggTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATcac                          >  5:571774/1‑150 (MQ=255)
                                                                                                                         ggTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATcac                          <  7:1251215/150‑1 (MQ=255)
                                                                                                                          gTATAGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACaa                        <  3:127303/151‑1 (MQ=255)
                                                                                                                           tataGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACaa                        >  5:233577/1‑150 (MQ=255)
                                                                                                                            ataGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAAt                       <  8:777352/150‑1 (MQ=255)
                                                                                                                            ataGGCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATc                      <  8:627236/151‑1 (MQ=255)
                                                                                                                              agtcTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCa                     <  7:460515/147‑1 (MQ=255)
                                                                                                                               ggCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGc                   >  4:285249/1‑151 (MQ=255)
                                                                                                                               ggCTTCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGc                   >  8:1663019/1‑151 (MQ=255)
                                                                                                                                  ttCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTc                >  2:156358/1‑151 (MQ=255)
                                                                                                                                   tCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCtt                 <  6:295941/149‑1 (MQ=255)
                                                                                                                                   tCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCtt                 <  7:1116340/149‑1 (MQ=255)
                                                                                                                                   tCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTc                <  6:1043513/150‑1 (MQ=255)
                                                                                                                                   tCGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGGCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTc                <  4:710969/150‑1 (MQ=255)
                                                                                                                                    cGGTGTATTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTcgc              >  8:1381640/1‑151 (MQ=255)
                                                                                                                                         tATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGt         >  3:179059/1‑151 (MQ=255)
                                                                                                                                          aTTCGCGGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGtt        <  5:1264441/151‑1 (MQ=255)
                                                                                                                                          aTTCGCGGTTTAATTTTTCATTGTTCAGCAGGTAAAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGtt        >  7:879319/1‑151 (MQ=255)
                                                                                                                                             cgcgGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTtgtg     >  1:557641/1‑151 (MQ=255)
                                                                                                                                               cgGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGa    >  4:911295/1‑150 (MQ=255)
                                                                                                                                               cgGTTTAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCCTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGa    >  4:143889/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAAT  >  NC_000913/4084784‑4085078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑