Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,219,752 C→T S386F (TCC→TTC)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,219,7520CT97.3% 1519.0 / 18.3 447S386F (TCC→TTC) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base T (213/222);  total (218/229)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 452 total aligned reads displayed.

TCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCG  >  NC_000913/4219605‑4219899
                                                                                                                                                   |                                                                                                                                                   
 catcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTtccc                                                                                                                                                  <  4:1309817/150‑1 (MQ=255)
  atcaAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAGAACTTTGTGCTGGAGAAGCGGCATATTTTCCCgg                                                                                                                                                >  4:235544/1‑151 (MQ=255)
     aaaGAGAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAt                                                                                                                                             <  5:1143173/151‑1 (MQ=255)
     aaaGACAGGGTCGCGCCGCAGAAAGAGATGGCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCAt                                                                                                                                             <  1:822995/151‑1 (MQ=255)
      aaGAGAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCa                                                                                                                                              <  3:669155/149‑1 (MQ=255)
      aaGACAGGTTCGCCCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGCGCACGATCGCGTGGGCCGAATGGCGGACACCCACGAGATTGAAAACTTTGTGCTGGAGAACCGGCAGATTTTCCCGGGAtt                                                                                                                                            >  1:986921/1‑151 (MQ=255)
       aGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCAtt                                                                                                                                            >  7:11310/1‑150 (MQ=255)
       aGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTa                                                                                                                                           <  1:1023586/151‑1 (MQ=255)
        gACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTaa                                                                                                                                          <  1:1561692/151‑1 (MQ=255)
         aCAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAAt                                                                                                                                         >  6:861641/1‑151 (MQ=255)
          cAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATg                                                                                                                                        >  5:34461/1‑151 (MQ=255)
               tCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGCACCCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAAtt                                                                                                                                   >  2:705070/1‑151 (MQ=255)
                cgcgCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAAtt                                                                                                                                   <  5:73720/150‑1 (MQ=255)
                   gccgcAGAAAGAGATGTCTGCGGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTg                                                                                                                               <  8:60538/151‑1 (MQ=255)
                     cgcAGAAAGCGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGATAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCt                                                                                                                             <  3:1459449/151‑1 (MQ=255)
                       cAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTc                                                                                                                           >  1:1132977/1‑151 (MQ=255)
                         gAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTc                                                                                                                           >  6:1471145/1‑149 (MQ=255)
                          aaaGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAgg                                                                                                                        >  1:4092/1‑151 (MQ=255)
                           aaGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGa                                                                                                                       <  8:1543793/151‑1 (MQ=255)
                            agagATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGaa                                                                                                                      <  3:129619/151‑1 (MQ=255)
                            agagATGTCTGCCACTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGaa                                                                                                                      <  3:1027557/151‑1 (MQ=255)
                            agagATGGCTGCCGCGCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGaa                                                                                                                      <  6:328072/151‑1 (MQ=255)
                             gagaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGa                                                                                                                       >  7:1030357/1‑149 (MQ=255)
                              agaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAagc                                                                                                                    <  5:79259/151‑1 (MQ=255)
                               gaTGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAagca                                                                                                                   <  4:1027285/151‑1 (MQ=255)
                                 tGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATCTTTTCCCGGCATTAATGGAATTACTGCTTCAGGAagcagc                                                                                                                 >  7:1478783/1‑151 (MQ=255)
                                      gCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaa                                                                                                             <  2:1135632/150‑1 (MQ=255)
                                      gCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGa                                                                                                              <  1:1431552/149‑1 (MQ=255)
                                      gCCGCTCACGTTCGAGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCgg                                                                                                               <  8:222134/148‑1 (MQ=255)
                                       ccGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaaaa                                                                                                           <  2:757490/151‑1 (MQ=255)
                                       ccGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGCATATTTTCGCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaaaaa                                                                                                          <  2:1277732/151‑1 (MQ=255)
                                        cGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGaaaaa                                                                                                          <  7:829770/151‑1 (MQ=255)
                                              cGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCAcc                                                                                                    <  5:216805/151‑1 (MQ=255)
                                              cGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCAcc                                                                                                    >  5:614818/1‑151 (MQ=255)
                                               gTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCg                                                                                                   >  7:529086/1‑151 (MQ=255)
                                               gTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCg                                                                                                   <  6:714859/151‑1 (MQ=255)
                                               gTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCg                                                                                                   >  4:1134292/1‑151 (MQ=255)
                                               gTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCg                                                                                                   >  8:40135/1‑151 (MQ=255)
                                                  cGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAt                                                                                                 <  3:1225796/150‑1 (MQ=255)
                                                   gTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAtct                                                                                               <  8:1040903/151‑1 (MQ=255)
                                                   gTGCCGGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAtct                                                                                               <  4:410053/151‑1 (MQ=255)
                                                    tGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCGGGGATTAATGGAATTACTGCTTCAGGAAGCAGCGGAATAAATCACCGAtctc                                                                                              >  2:315653/1‑151 (MQ=255)
                                                    tGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAtctc                                                                                              >  3:246060/1‑151 (MQ=255)
                                                     gCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGAtctc                                                                                              <  3:1120961/150‑1 (MQ=255)
                                                     gCCTGCTATCAACTGGTGAAAGAGCACGATCGCGGGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCGGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCg                                                                                             <  3:965893/151‑1 (MQ=255)
                                                         gCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGa                                                                                         <  2:1528122/151‑1 (MQ=255)
                                                          cTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGa                                                                                         >  2:1401901/1‑150 (MQ=255)
                                                            aTCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAAc                                                                                       >  7:272305/1‑150 (MQ=255)
                                                            aTCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAAc                                                                                       <  5:1275246/150‑1 (MQ=255)
                                                             tCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACa                                                                                      >  2:533174/1‑150 (MQ=255)
                                                              cAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACaaa                                                                                    <  4:1492334/151‑1 (MQ=255)
                                                                aCTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAAtt                                                                                  >  5:912283/1‑151 (MQ=255)
                                                                aCTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAAtt                                                                                  <  5:1678532/151‑1 (MQ=255)
                                                                aCTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAAtt                                                                                  >  6:464367/1‑151 (MQ=255)
                                                                          gagCACGATCGCGTGGGTCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGcc                                                                        >  1:637332/1‑151 (MQ=255)
                                                                          gagCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGcc                                                                        <  7:45077/151‑1 (MQ=255)
                                                                            gCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCAt                                                                      >  8:495621/1‑151 (MQ=255)
                                                                                gATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCttt                                                                  >  5:1400990/1‑151 (MQ=255)
                                                                                   cgcgTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTtata                                                               <  5:1525768/151‑1 (MQ=255)
                                                                                    gcgTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTatat                                                              <  6:924677/151‑1 (MQ=255)
                                                                                     cgTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATAtt                                                             <  3:2936/151‑1 (MQ=255)
                                                                                       tGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTg                                                            >  1:1677591/1‑150 (MQ=255)
                                                                                         ggCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAgc                                                         >  4:556701/1‑151 (MQ=255)
                                                                                            cGAAGGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTATATTGAgcggcg                                                     <  2:878551/151‑1 (MQ=255)
                                                                                             gAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAgcggcg                                                     <  4:1408653/151‑1 (MQ=255)
                                                                                               agggCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGcggcgg                                                    >  7:625147/3‑150 (MQ=255)
                                                                                               aTGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGcggcgg                                                    >  8:1226441/1‑150 (MQ=255)
                                                                                               aTGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGa                                                   <  6:544576/151‑1 (MQ=255)
                                                                                                 ggCGGAGACCCAGGAGTTTGAAAAGTTTGTTCTTGAGAAGCGGCATATTTTCCCGGCATTAATGTAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGAt                                                  <  6:1124912/150‑1 (MQ=255)
                                                                                                 ggCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGAt                                                  <  7:1377093/150‑1 (MQ=255)
                                                                                                  gCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATgg                                                <  6:1374869/151‑1 (MQ=255)
                                                                                                   cGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGt                                               <  3:486021/151‑1 (MQ=255)
                                                                                                   cGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGt                                               <  1:678532/151‑1 (MQ=255)
                                                                                                        acCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGc                                          <  8:696226/151‑1 (MQ=255)
                                                                                                              gAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAat                                     >  8:873376/1‑150 (MQ=255)
                                                                                                                gTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATc                                  <  8:1452676/151‑1 (MQ=255)
                                                                                                                  ttGAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATct                                 <  4:736667/150‑1 (MQ=255)
                                                                                                                    gAAAACTTTTTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCTCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctgg                               <  6:1568796/150‑1 (MQ=255)
                                                                                                                    gAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                              <  5:96474/151‑1 (MQ=255)
                                                                                                                    gAAAACTTTGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATctggc                              >  7:75633/1‑151 (MQ=255)
                                                                                                                           ttGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAAc                        >  1:304899/1‑150 (MQ=255)
                                                                                                                           ttGTGCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAAc                        <  3:1622455/150‑1 (MQ=255)
                                                                                                                            tgtgCTGGATAAGCGGCATATTTTCCCGGCATTATTGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACa                       <  2:20669/150‑1 (MQ=255)
                                                                                                                             gtgCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAg                     <  2:457481/151‑1 (MQ=255)
                                                                                                                               gCTGGAGAAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTg                   <  4:654275/151‑1 (MQ=255)
                                                                                                                                    agaAGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCCCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAgg              >  4:1614442/1‑151 (MQ=255)
                                                                                                                                      aaGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGTCGAACAAATTGTGATTCGCCATCTTTATATTGCTCTGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTc            <  4:961038/151‑1 (MQ=255)
                                                                                                                                      aaGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTc            >  3:380186/1‑151 (MQ=255)
                                                                                                                                       aGCGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTca           <  4:483821/151‑1 (MQ=255)
                                                                                                                                         cGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGGTGAATATCTGGCTGGAACAAGTGGAAGGTcagc         >  6:1664940/1‑151 (MQ=255)
                                                                                                                                         cGGCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTcagc         >  1:499860/1‑151 (MQ=255)
                                                                                                                                           gCATATTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTcagcag       <  8:1394731/151‑1 (MQ=255)
                                                                                                                                           gCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTcagcag       <  5:1054230/151‑1 (MQ=255)
                                                                                                                                             atatTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGtt     <  7:876776/151‑1 (MQ=255)
                                                                                                                                             atatTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGtt     <  7:1279676/151‑1 (MQ=255)
                                                                                                                                               atTTTCCCGGCATTAATGGAATTACTGCTTCATGATGCATCGGAAAAAATCACAGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTg    <  4:814674/150‑1 (MQ=255)
                                                                                                                                               atTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTgc   <  6:1290834/151‑1 (MQ=255)
                                                                                                                                               atTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCGGGAACAAGTGGAAGGTCAGCAGTTgc   <  4:1153455/151‑1 (MQ=255)
                                                                                                                                               atTTTCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAACGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTg    <  3:634453/150‑1 (MQ=255)
                                                                                                                                               atTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTgc   <  5:4107/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCGGATGGTGCCGCTCAATATCTGGCTGGAACAAGTGGAAGGTCAGCAGTTGCG  >  NC_000913/4219605‑4219899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: