| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,224,483 | C→T | S219F (TCC→TTC) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,224,483 | 0 | C | T | 97.7% | 1344.8 / 12.2 | 395 | S219F (TCC→TTC) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (6/3); new base T (219/165); total (227/168) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
Only 100 of 396 total aligned reads displayed.
GCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTA > NC_000913/4224334‑4224630 | gcTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTcc > 8:190699/1‑151 (MQ=255) ggtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCggg < 7:734488/151‑1 (MQ=255) ggtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCggg < 3:867103/151‑1 (MQ=255) ggtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCgg < 7:1130302/150‑1 (MQ=255) gtggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGgc > 5:1079757/1‑151 (MQ=255) ggAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGgcgc > 2:1236638/1‑151 (MQ=255) aaGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGCGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCAc > 1:1131742/1‑151 (MQ=255) aaGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCAc > 3:1653651/1‑151 (MQ=255) ggCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGctct > 8:1597147/1‑151 (MQ=255) gcgcGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCtctc > 8:841173/1‑151 (MQ=255) cgcgGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTcc < 8:743108/151‑1 (MQ=255) ggATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCggg < 5:1205329/151‑1 (MQ=255) aTCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGc > 5:413969/1‑150 (MQ=255) cTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGc > 4:1659347/1‑148 (MQ=255) gATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGacc < 8:47648/151‑1 (MQ=255) gATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGa < 8:1157223/149‑1 (MQ=255) aTCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGacc > 4:1295284/1‑150 (MQ=255) aTCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGacc > 1:764355/1‑150 (MQ=255) ccTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGAGCGCACGCTCTCCGGGCAGaccacc < 5:1464041/151‑1 (MQ=255) cTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCg > 1:217469/1‑151 (MQ=255) aTTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGa > 8:296741/1‑149 (MQ=255) ttGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGc > 1:255739/1‑151 (MQ=255) tGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGc > 1:544990/1‑150 (MQ=255) gAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAt > 4:823034/1‑151 (MQ=255) aCCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAtttt < 5:1528741/151‑1 (MQ=255) ttttCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACa > 6:128051/1‑150 (MQ=255) tttCGACAGCCTTAAAGCCAAAGAGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCTTTGAGCTGGCGATTATGATCTCGGGCACCATCACCGACTCATTCGGGCGCAGGCTCTCCGGGCAGACAACCGAAGCATTTTACatc > 6:1383003/1‑149 (MQ=255) tttCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACaa < 4:277032/150‑1 (MQ=255) tttCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAAc > 4:904722/1‑151 (MQ=255) tCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTc > 2:1343210/1‑151 (MQ=255) acacCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCAtt > 2:1639547/1‑151 (MQ=255) acCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTgc > 6:1082607/1‑151 (MQ=255) ccTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTgcgc > 1:400800/1‑151 (MQ=255) tAACGCGAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGAGGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCa < 1:1645550/150‑1 (MQ=255) aaCGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGCCGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACg > 2:988397/1‑151 (MQ=255) aCGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGc > 8:543732/1‑151 (MQ=255) cGCCAAAGCTGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGcc < 2:527313/151‑1 (MQ=255) cGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGcc > 5:1368621/1‑151 (MQ=255) gCCACAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCg < 6:1479872/151‑1 (MQ=255) ccAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCg > 1:1344347/1‑150 (MQ=255) ccAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCg > 3:340760/1‑150 (MQ=255) cAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCCCCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGACACGCCgc > 1:472503/1‑149 (MQ=255) cAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGaa < 5:617476/151‑1 (MQ=255) cAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGaa < 4:1516857/151‑1 (MQ=255) aaaGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAg < 7:882818/151‑1 (MQ=255) aaGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGc > 8:1002343/1‑151 (MQ=255) cggcggTAGTTGCGCTGAAAACGGAGTTTGAAGCGCTGGGCGTGGAGCTGCCGAGTATGATCTCCCGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAATTTACAACTCATTGCGCCACGCCGAAGctct < 2:326766/151‑1 (MQ=255) cggTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCAATTTACAACTCATTGCGCCACTCCGAAGCTCTGAc > 6:785442/1‑151 (MQ=255) gTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCt > 7:1029989/1‑151 (MQ=255) cGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCt > 4:55770/1‑151 (MQ=255) ggTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTg < 1:1019280/151‑1 (MQ=255) gTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGAGCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGa < 2:1512083/151‑1 (MQ=255) tGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGa > 3:567196/1‑150 (MQ=255) tGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGa > 5:948262/1‑150 (MQ=255) gAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTCAAc > 6:203061/1‑151 (MQ=255) aaaaCGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACt < 5:742651/151‑1 (MQ=255) aaCGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCGCTCCGGGCAGACCACCGAAGCATTTAACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACtgt < 2:771519/151‑1 (MQ=255) ggAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTgcg > 3:1090969/1‑151 (MQ=255) gAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTgcgc > 2:1088733/1‑151 (MQ=255) aGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCt > 7:554427/1‑151 (MQ=255) gTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTg < 1:969029/151‑1 (MQ=255) gTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTg < 1:105204/151‑1 (MQ=255) ttGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTggg > 5:1647062/1‑151 (MQ=255) tGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTgggg > 7:1465834/1‑151 (MQ=255) tGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTgggg > 1:860444/1‑151 (MQ=255) tGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTggg < 2:817376/150‑1 (MQ=255) tGAAGCACTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGCCCTTTGGCCTGAACTGTGCGCTgggg > 5:225702/1‑151 (MQ=255) aaGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGcc < 1:641607/151‑1 (MQ=255) aaGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGcc > 2:649366/1‑151 (MQ=255) gcgcTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCg < 8:1547816/151‑1 (MQ=255) tGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCCCCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGCCCTTGGGCCTGAACTGTGCGCTGGGGCCCGATg > 6:548437/1‑150 (MQ=255) gggCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGac > 8:678625/1‑150 (MQ=255) gggCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGaa < 5:836564/151‑1 (MQ=255) cGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTg > 8:1272900/1‑151 (MQ=255) gAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTgcgc < 6:1223603/150‑1 (MQ=255) gCCGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTAc > 4:420629/1‑151 (MQ=255) cGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGCCCACCGAAGCATTTTACAACTCATTGCGCCACGCCGCAGCTCTGCCCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGt > 2:263142/1‑151 (MQ=255) cGATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCGCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGt < 2:1180530/151‑1 (MQ=255) gATTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTg < 8:422112/151‑1 (MQ=255) aTTATGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGc > 7:3258/1‑151 (MQ=255) aTGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAg < 5:543588/150‑1 (MQ=255) aTGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAg < 1:159754/150‑1 (MQ=255) tGATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAgg > 3:1445943/1‑150 (MQ=255) gATCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGa < 7:450270/150‑1 (MQ=255) aTCTCCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGc > 6:945069/1‑151 (MQ=255) gctcCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCt < 7:1291353/150‑1 (MQ=255) tcCGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGt > 7:184831/1‑151 (MQ=255) ccGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGTCCTGAACTGAGAGCAGGGGCCCGATGAACTGCGCCAATACGTACAGCAGCtct > 6:676386/1‑148 (MQ=255) ccGGCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGt < 5:277729/150‑1 (MQ=255) cGGCACCATAACCGACGCCTTCGGGCGCACGCTCTCCGGCCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCCGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTcc > 6:1060229/1‑150 (MQ=255) gCACCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACg > 7:242215/1‑151 (MQ=255) aCCATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGa < 4:1187545/151‑1 (MQ=255) cATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAGCTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGAtt < 8:1416339/151‑1 (MQ=255) cATCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGAtt < 3:1241234/151‑1 (MQ=255) tCACCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGc > 6:290488/1‑151 (MQ=255) cACCGTCGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCGCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGc < 3:472183/150‑1 (MQ=255) aCCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGACCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCgg > 6:1595227/1‑151 (MQ=255) aCCGACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCgg < 4:1561690/151‑1 (MQ=255) gACGCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAAt < 4:1033678/151‑1 (MQ=255) gCCTTCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGACCTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGGATGCt > 4:1427400/1‑151 (MQ=255) | GCTGGTGGAAGGTGGCGCGGATCTGATCCTGATTGAAACCGTTTTCGACACCCTTAACGCCAAAGCGGCGGTATTTGCGGTGAAAACGGAGTTTGAAGCGCTGGGCGTTGAGCTGCCGATTATGATCTCCGGCACCATCACCGACGCCTCCGGGCGCACGCTCTCCGGGCAGACCACCGAAGCATTTTACAACTCATTGCGCCACGCCGAAGCTCTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTA > NC_000913/4224334‑4224630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |