Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,234,479 C→T A241V (GCG→GTG)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,234,4790CT97.1% 1407.7 / 18.7 414A241V (GCG→GTG) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base C (9/3);  new base T (209/192);  total (218/196)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 419 total aligned reads displayed.

GTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTT  >  NC_000913/4234331‑4234626
                                                                                                                                                    |                                                                                                                                                   
     gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGgcgc                                                                                                                                                <  3:87221/149‑1 (MQ=255)
     gAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCtt                                                                                                                                              >  5:1260664/1‑151 (MQ=255)
      aaaaaaGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCtt                                                                                                                                              <  6:1166381/150‑1 (MQ=255)
           aGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccacc                                                                                                                                        >  6:549084/1‑151 (MQ=255)
            gTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccacca                                                                                                                                       <  6:86301/151‑1 (MQ=255)
             tAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGGTTTccacca                                                                                                                                       >  8:734544/1‑150 (MQ=255)
              aaaCCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTccacca                                                                                                                                       <  2:1427510/149‑1 (MQ=255)
                 cccGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGcc                                                                                                                                  >  4:979688/1‑151 (MQ=255)
                   cGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCa                                                                                                                                 >  2:504295/1‑150 (MQ=255)
                    ggAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCaaa                                                                                                                               <  2:1339365/151‑1 (MQ=255)
                     gAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCaa                                                                                                                                >  2:183770/1‑149 (MQ=255)
                     gAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAg                                                                                                                              <  6:973491/151‑1 (MQ=255)
                       aaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGcc                                                                                                                            <  3:556791/151‑1 (MQ=255)
                       aaCCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGcc                                                                                                                            >  1:1158656/1‑151 (MQ=255)
                       aaCCACGCTGTGCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGcc                                                                                                                            <  7:928284/151‑1 (MQ=255)
                          cACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGtt                                                                                                                         >  5:600332/1‑151 (MQ=255)
                          cACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGtt                                                                                                                         <  2:67919/151‑1 (MQ=255)
                           aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                                        >  8:908333/1‑151 (MQ=255)
                           aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                                        <  5:1526809/151‑1 (MQ=255)
                           aCGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTg                                                                                                                        >  8:1339435/1‑151 (MQ=255)
                            cGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                                       >  2:773891/1‑151 (MQ=255)
                            cGCTGTTCTTGGTAGCATCTAAAACCTTCACCAATCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTgg                                                                                                                       <  8:188407/151‑1 (MQ=255)
                             gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                      >  5:190401/1‑151 (MQ=255)
                             gCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGc                                                                                                                      >  5:269207/1‑151 (MQ=255)
                               tGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCg                                                                                                                     >  3:1661352/1‑150 (MQ=255)
                                  tCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGt                                                                                                                  <  2:285049/150‑1 (MQ=255)
                                  tCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCGTTCCACCAATGCCAAAGCCGTTGGCGAGtt                                                                                                                 >  4:313421/1‑151 (MQ=255)
                                   cTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                                                                                                >  7:214868/1‑151 (MQ=255)
                                   cTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGttt                                                                                                                >  5:355555/1‑151 (MQ=255)
                                     tGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTg                                                                                                               <  7:638124/150‑1 (MQ=255)
                                      ggTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                                             >  2:1543539/1‑151 (MQ=255)
                                       gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGt                                                                                                             <  1:1405925/150‑1 (MQ=255)
                                       gTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTa                                                                                                            <  7:1145394/151‑1 (MQ=255)
                                         aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                                           <  3:40678/150‑1 (MQ=255)
                                         aGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTAt                                                                                                           <  1:1667873/150‑1 (MQ=255)
                                            aTCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                                       >  8:359845/1‑151 (MQ=255)
                                             tCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                                       >  6:329869/1‑150 (MQ=255)
                                              cTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATAc                                                                                                     >  5:872936/1‑151 (MQ=255)
                                               tAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACt                                                                                                    >  2:1051699/1‑151 (MQ=255)
                                                aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGAt                                                                                                       <  7:790692/147‑1 (MQ=255)
                                                aaaaCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTg                                                                                                   <  8:1375345/151‑1 (MQ=255)
                                                   aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                                                <  7:284568/151‑1 (MQ=255)
                                                   aCCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGAGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTAGCAAAACACTATGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCa                                                                                                <  2:1138292/151‑1 (MQ=255)
                                                      ttCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAAc                                                                                              >  1:927827/1‑150 (MQ=255)
                                                        caccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATg                                                                                           <  4:1268939/151‑1 (MQ=255)
                                                        caccacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATg                                                                                           <  1:1244670/151‑1 (MQ=255)
                                                           cacTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTc                                                                                        >  5:1016165/1‑151 (MQ=255)
                                                              acAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                     <  3:1634247/150‑1 (MQ=255)
                                                               cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                     <  3:1413623/150‑1 (MQ=255)
                                                               cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                     <  3:1584542/150‑1 (MQ=255)
                                                               cAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAg                                                                                     <  5:1444816/150‑1 (MQ=255)
                                                                aGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAGAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGtt                                                                                   >  3:347249/1‑151 (MQ=255)
                                                                 ggAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTc                                                                                  <  4:752483/151‑1 (MQ=255)
                                                                              aaCGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTg                                                                     <  5:459865/151‑1 (MQ=255)
                                                                                 gCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcg                                                                  <  4:1217174/151‑1 (MQ=255)
                                                                                  cccATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTggcgg                                                                 >  8:1620844/1‑151 (MQ=255)
                                                                                          gcgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAct                                                         <  8:443557/151‑1 (MQ=255)
                                                                                           cgcgTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTActc                                                        >  3:128970/1‑151 (MQ=255)
                                                                                              gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCttt                                                     <  8:502336/151‑1 (MQ=255)
                                                                                              gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCttt                                                     >  8:1486910/1‑151 (MQ=255)
                                                                                              gTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCttt                                                     <  8:591291/151‑1 (MQ=255)
                                                                                                 aCTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                                  <  1:512946/151‑1 (MQ=255)
                                                                                                  cTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTgg                                                 >  1:261047/1‑151 (MQ=255)
                                                                                                   tGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGt                                                >  7:1323094/1‑151 (MQ=255)
                                                                                                    ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                               <  1:356536/151‑1 (MQ=255)
                                                                                                    ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                               <  7:979791/151‑1 (MQ=255)
                                                                                                    ggTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTc                                               <  1:1352445/151‑1 (MQ=255)
                                                                                                     gTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAAGACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCa                                              <  1:1054532/151‑1 (MQ=255)
                                                                                                         cTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCg                                           >  6:441356/1‑150 (MQ=255)
                                                                                                          tGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                                         >  3:945281/1‑151 (MQ=255)
                                                                                                           gAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGTGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                                          <  2:11703/149‑1 (MQ=255)
                                                                                                           gAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                                          <  5:663297/149‑1 (MQ=255)
                                                                                                           gAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCCTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                                         <  8:295178/150‑1 (MQ=255)
                                                                                                           gAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGATTTCCACCAATGCCATAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAt                                         <  6:901377/150‑1 (MQ=255)
                                                                                                            aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGGTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTg                                       <  8:1217615/151‑1 (MQ=255)
                                                                                                            aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTtgtg                                                  <  6:1391459/140‑1 (MQ=255)
                                                                                                             aaGCGGCAGGTGATGAAAAACACGTTGCAAAAGAGTTTGTGGGGCTTTCCACCAATGCGAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTgg                                      <  4:894240/151‑1 (MQ=255)
                                                                                                             aaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTgg                                      >  6:1605600/1‑151 (MQ=255)
                                                                                                               gCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCGACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGcc                                    >  2:885132/1‑151 (MQ=255)
                                                                                                                 ggCAGGTGATGAAAAGCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                  >  3:1208125/1‑151 (MQ=255)
                                                                                                                  gCAGGTGATGAAAAACACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGt                                 >  4:487791/1‑151 (MQ=255)
                                                                                                                             aaaaCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctc                      >  7:1419571/1‑151 (MQ=255)
                                                                                                                              aaaCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctct                     >  2:1253605/1‑151 (MQ=255)
                                                                                                                              aaaCACGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctct                     >  4:1106322/1‑151 (MQ=255)
                                                                                                                                acacGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTAGTCTTTGTGGTCAGCGATTGGCCTGTCGATGTTTCTCTcc                   >  7:1559194/1‑151 (MQ=255)
                                                                                                                                   cGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                <  5:1139450/151‑1 (MQ=255)
                                                                                                                                   cGTTGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                >  4:152838/1‑151 (MQ=255)
                                                                                                                                     ttGCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCgg              >  1:290488/1‑151 (MQ=255)
                                                                                                                                       gCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGc             <  3:785323/150‑1 (MQ=255)
                                                                                                                                       gCAAAACACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGc             <  8:822962/150‑1 (MQ=255)
                                                                                                                                        cataaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCTACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCtt           <  6:780978/148‑1 (MQ=255)
                                                                                                                                           aaCACTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGa        <  7:1078386/151‑1 (MQ=255)
                                                                                                                                            acacTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt       <  1:1526493/151‑1 (MQ=255)
                                                                                                                                              acTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATaa     <  4:1323296/151‑1 (MQ=255)
                                                                                                                                              acTTTGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATaa     <  3:112538/151‑1 (MQ=255)
                                                                                                                                                tttGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt   >  4:200903/1‑151 (MQ=255)
                                                                                                                                                tttGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt   >  8:142969/1‑151 (MQ=255)
                                                                                                                                                tttGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACt   >  1:674349/1‑151 (MQ=255)
                                                                                                                                                tttGTGCCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGCTTGGAGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTATCTCCATCGGCTTTGATaagt   >  6:1523831/1‑149 (MQ=255)
                                                                                                                                                 ttGTGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAAc    <  8:663104/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTT  >  NC_000913/4234331‑4234626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: