Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,265,668 C→T T452I (ACC→ATC)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,265,6680CT95.2% 1631.8 / 43.7 509T452I (ACC→ATC) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (16/8);  new base T (248/232);  total (269/240)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 510 total aligned reads displayed.

CGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCG  >  NC_000913/4265520‑4265815
                                                                                                                                                    |                                                                                                                                                   
cGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGAGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCt                                                                                                                                                   >  4:877810/1‑151 (MQ=255)
 gTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCtt                                                                                                                                                  <  7:1344635/151‑1 (MQ=255)
    aaTCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTaa                                                                                                                                               <  7:1601709/151‑1 (MQ=255)
    aaTCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTa                                                                                                                                                >  4:1363634/1‑150 (MQ=255)
          gCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTc                                                                                                                                          >  7:956084/1‑150 (MQ=255)
              tATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATGGGGACGGTACGCCTGATCTTTAACGGTCAATgg                                                                                                                                     >  8:1212867/1‑151 (MQ=255)
               aTCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGt                                                                                                                                    >  1:926419/1‑151 (MQ=255)
               aTCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATg                                                                                                                                      <  8:393952/149‑1 (MQ=255)
                 cGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTcg                                                                                                                                  <  1:704422/151‑1 (MQ=255)
                  gAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTCCGCCTGATCTTTAACGGTCAATGGTcgc                                                                                                                                 >  3:1374384/1‑151 (MQ=255)
                  gAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTcgc                                                                                                                                 <  4:796242/151‑1 (MQ=255)
                     cAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCt                                                                                                                              >  5:962376/1‑151 (MQ=255)
                       ggATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTc                                                                                                                            >  6:180607/1‑151 (MQ=255)
                       ggATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTc                                                                                                                            <  4:38031/151‑1 (MQ=255)
                       ggATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTc                                                                                                                            <  5:352762/151‑1 (MQ=255)
                            cGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACa                                                                                                                        >  4:713662/1‑150 (MQ=255)
                            cGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACa                                                                                                                        >  5:1099842/1‑150 (MQ=255)
                               aCTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACt                                                                                                                     >  3:57916/1‑150 (MQ=255)
                                 ttGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCGTCGACAACTATg                                                                                                                  >  1:963943/1‑151 (MQ=255)
                                  tGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTAt                                                                                                                   <  5:680289/149‑1 (MQ=255)
                                  tGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTAt                                                                                                                   <  2:808554/149‑1 (MQ=255)
                                  tGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGc                                                                                                                 >  7:312776/1‑151 (MQ=255)
                                  tGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATg                                                                                                                  <  6:669045/150‑1 (MQ=255)
                                  tGATCATGTTTATCTATCGCGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTAt                                                                                                                   <  7:390083/149‑1 (MQ=255)
                                   gctCATGTTTATCTATCCTGATGAGGTGTTTCAAGAAAACCGTTATTTAAATTGGATCGCGCAAATTATTATCGGTAAACCACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGc                                                                                                                 <  1:153181/148‑1 (MQ=255)
                                    aTCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCgg                                                                                                               >  7:727279/1‑151 (MQ=255)
                                     tCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCggg                                                                                                              >  5:780792/1‑151 (MQ=255)
                                     tCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCggg                                                                                                              >  8:858840/1‑151 (MQ=255)
                                       aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGc                                                                                                            >  2:987074/1‑151 (MQ=255)
                                       aTGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGc                                                                                                            >  3:476671/1‑151 (MQ=255)
                                        tGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGcc                                                                                                           >  6:809779/1‑151 (MQ=255)
                                         gTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCg                                                                                                          >  2:114033/1‑151 (MQ=255)
                                               ctatcGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTac                                                                                                    >  4:172117/1‑151 (MQ=255)
                                                tatcGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacg                                                                                                   >  5:590736/1‑151 (MQ=255)
                                                 atcGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTac                                                                                                    <  1:807787/149‑1 (MQ=255)
                                                 atcGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTac                                                                                                    <  3:1272164/149‑1 (MQ=255)
                                                 atcGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTa                                                                                                     <  3:1491363/148‑1 (MQ=255)
                                                   cGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacg                                                                                                <  1:543246/151‑1 (MQ=255)
                                                   cGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacg                                                                                                >  5:1049607/1‑151 (MQ=255)
                                                   cGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacg                                                                                                >  1:1211436/1‑151 (MQ=255)
                                                      gatgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgac                                                                                              <  7:661883/150‑1 (MQ=255)
                                                      gatgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTAAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTccgacgacg                                                                                             >  7:103672/1‑151 (MQ=255)
                                                        tgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACTGTACGCCTGAACTTTAACGTTCAATGATCGCGCTTCGACAACTATGCGGGGCCGCAGTAcgacgacga                                                                                            >  6:999598/1‑150 (MQ=255)
                                                        tgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTAcgacgacga                                                                                            >  6:1179414/1‑150 (MQ=255)
                                                          aGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaata                                                                                         <  5:147062/151‑1 (MQ=255)
                                                           ggTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaata                                                                                         >  4:1134201/1‑150 (MQ=255)
                                                            gtgtATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataat                                                                                       <  8:1075163/151‑1 (MQ=255)
                                                                aTCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataataat                                                                                    <  2:706214/150‑1 (MQ=255)
                                                                 tCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAatt                                                                                   >  8:1696204/1‑150 (MQ=255)
                                                                  cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAatta                                                                                  <  4:248902/150‑1 (MQ=255)
                                                                   aCGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAattatt                                                                                <  8:268441/151‑1 (MQ=255)
                                                                     gAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTAtttt                                                                              <  4:1475600/151‑1 (MQ=255)
                                                                      aaaaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTa                                                                             <  8:475331/151‑1 (MQ=255)
                                                                        aaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATg                                                                           <  4:1572740/151‑1 (MQ=255)
                                                                         aCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGa                                                                          <  5:900082/151‑1 (MQ=255)
                                                                           aGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAAt                                                                        <  5:530348/151‑1 (MQ=255)
                                                                                   aaaaGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTaa                                                                 <  3:1151473/150‑1 (MQ=255)
                                                                                     aaGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAAtt                                                               >  3:969737/1‑150 (MQ=255)
                                                                                      aGGCATCGCGGAAATTATTATCGGTAAACCTCGTAACGGCCCAATCGGGACGGTACGCCTGACCATTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTaa                                                             <  3:268058/151‑1 (MQ=255)
                                                                                      aGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATTCGGGGCCGCAGGACGACGACGAATAATAATTATTTTATGAATTAGGTAATTa                                                              >  7:594303/1‑150 (MQ=255)
                                                                                        gCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAg                                                           <  2:1580610/151‑1 (MQ=255)
                                                                                         cATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc                                                          <  6:437558/151‑1 (MQ=255)
                                                                                         cATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc                                                          >  7:1267324/1‑151 (MQ=255)
                                                                                          aTCGCGGAAATTATTATCGGTAAACAACGAAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCa                                                         <  2:1267395/151‑1 (MQ=255)
                                                                                            ggcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCTCGCTTCGACAACTATGCTGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaaa                                                       <  8:154452/150‑1 (MQ=255)
                                                                                            cgcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaa                                                        >  6:1473436/1‑150 (MQ=255)
                                                                                            cgcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaa                                                        >  6:507496/1‑150 (MQ=255)
                                                                                            cgcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaa                                                        <  8:778878/150‑1 (MQ=255)
                                                                                            cgcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCGGATCTTTAACGGTCAATGGTCGCGCTTCGCCAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTTATTAACGCaa                                                        >  2:197436/1‑150 (MQ=255)
                                                                                             gcgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAac                                                      >  7:513785/1‑151 (MQ=255)
                                                                                               ggAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAacac                                                    >  6:138997/1‑151 (MQ=255)
                                                                                               ggAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAacac                                                    >  5:266111/1‑151 (MQ=255)
                                                                                                 aaaTTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACtt                                                  >  6:1235817/1‑151 (MQ=255)
                                                                                                 aaaTTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACtt                                                  <  5:1370823/151‑1 (MQ=255)
                                                                                                  aaTTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTa                                                 <  5:1581658/151‑1 (MQ=255)
                                                                                                   attattATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTATAACGGTCAATGGTCGCGCATCGACAACTATGCGGGGCCGCACTACGACGACGAATAATAATTATTTTATGAATTAGATAATTAAAGCAAACACTTAt                                                >  1:319490/1‑151 (MQ=255)
                                                                                                     tattatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATc                                               <  6:301666/150‑1 (MQ=255)
                                                                                                      attatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCa                                              <  7:216652/150‑1 (MQ=255)
                                                                                                        tatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAg                                            <  8:69579/150‑1 (MQ=255)
                                                                                                           cGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAac                                        >  7:1472756/1‑151 (MQ=255)
                                                                                                            ggTAAACAACGTAACGGCCCAGTCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAaca                                       <  1:1620617/151‑1 (MQ=255)
                                                                                                             gTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAacac                                      <  4:533986/151‑1 (MQ=255)
                                                                                                              tAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAACGAacac                                      >  8:1438379/1‑150 (MQ=255)
                                                                                                               aaaCAACGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAAcaca                                     >  7:1168127/1‑150 (MQ=255)
                                                                                                                   aacGTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGc                                >  4:994599/1‑151 (MQ=255)
                                                                                                                    acGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGc                                >  2:1496197/1‑150 (MQ=255)
                                                                                                                      gTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCa                               >  7:860919/1‑149 (MQ=255)
                                                                                                                      gTAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAg                             <  6:356794/151‑1 (MQ=255)
                                                                                                                       tAACGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGc                            >  3:1621502/1‑151 (MQ=255)
                                                                                                                        aaCGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGc                            <  5:171544/150‑1 (MQ=255)
                                                                                                                        aaCGGCCCAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCg                           <  2:506973/151‑1 (MQ=255)
                                                                                                                              ccAATGGGGACGGTACGCGTGATCTTTAAGGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACAGTTATCATGGAACACAAATGCAAGGGGCAACt                     <  4:1043804/151‑1 (MQ=255)
                                                                                                                               cAATCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACtg                    <  1:1042801/151‑1 (MQ=255)
                                                                                                                                aaTCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTAGGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACtgt                   >  6:1142642/1‑151 (MQ=255)
                                                                                                                                 aTCGGGACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACtgtt                  >  1:1345234/1‑151 (MQ=255)
                                                                                                                                    gggACGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGAGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTtgtg               <  8:629784/151‑1 (MQ=255)
                                                                                                                                        cGGTACGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGAtt            >  2:359542/1‑150 (MQ=255)
                                                                                                                                            aCGCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAccg       <  1:418980/151‑1 (MQ=255)
                                                                                                                                              gCCTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAccgcc     >  4:1234079/1‑151 (MQ=255)
                                                                                                                                               ccTGATCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAAccgccg    <  5:1222714/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACCGCCGCG  >  NC_000913/4265520‑4265815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑