Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,284,077 C→T A276T (GCG→ACG)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,284,0770CT95.5% 1198.1 / 31.2 384A276T (GCG→ACG) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base C (9/8);  new base T (182/180);  total (192/192)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

Only 100 of 394 total aligned reads displayed.

GCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCAT  >  NC_000913/4283929‑4284224
                                                                                                                                                    |                                                                                                                                                   
  accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                  >  7:982510/1‑150 (MQ=255)
  accaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCcgtcgt                                                                                                                                                  >  5:1304202/1‑150 (MQ=255)
   ccaaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTAc                                                                                                                                                >  4:1246293/1‑151 (MQ=255)
    caaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTAcc                                                                                                                                               >  1:536796/1‑151 (MQ=255)
     aaccaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCa                                                                                                                                              <  4:186428/151‑1 (MQ=255)
      accaGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCaa                                                                                                                                             <  1:1028791/151‑1 (MQ=255)
        caGCATGATCGCTCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAAc                                                                                                                                           <  6:377441/151‑1 (MQ=255)
           cATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATc                                                                                                                                        <  1:110524/151‑1 (MQ=255)
            aTGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATc                                                                                                                                        >  3:336260/1‑150 (MQ=255)
            aTGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCa                                                                                                                                       <  8:303695/151‑1 (MQ=255)
              gATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAg                                                                                                                                      <  1:1254817/150‑1 (MQ=255)
                   cgcCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGCTGGCGTAGAACACGATCTTACGTCCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGACGTACCAAACATCAGTccc                                                                                                                                  >  2:1585151/1‑149 (MQ=255)
                    gcCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTcccagc                                                                                                                               >  4:944168/1‑150 (MQ=255)
                     ccGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTcccagac                                                                                                                              >  3:816083/1‑151 (MQ=255)
                      cGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATAGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCACTcccagacc                                                                                                                             >  5:280857/1‑151 (MQ=255)
                       gAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTcccagaccc                                                                                                                            >  6:1508928/1‑151 (MQ=255)
                       gAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTcccagacac                                                                                                                            >  3:1297662/1‑149 (MQ=255)
                             gATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCagagag                                                                                                                      <  6:305263/151‑1 (MQ=255)
                               taataaAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCagagag                                                                                                                      >  5:1140242/1‑149 (MQ=255)
                                aataaAGGTCGGAATATAGAAGTAGCCCATAAACCCGGTCGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACCTCAGTCCCAGACCCAGAGAGAgc                                                                                                                   >  4:457490/1‑151 (MQ=255)
                                aataaAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTTCCAAACATCAGTCCCAGAGCCAGAGAGAgc                                                                                                                   >  4:250446/1‑151 (MQ=255)
                                aataaAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAgc                                                                                                                   <  8:1179062/151‑1 (MQ=255)
                                 ataaAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAgcg                                                                                                                  >  8:1455088/1‑151 (MQ=255)
                                 ataaAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAgcg                                                                                                                  >  6:1308075/1‑151 (MQ=255)
                                   aaaGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAgcgc                                                                                                                 <  2:1376187/150‑1 (MQ=255)
                                     aGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGga                                                                                                              <  1:149727/151‑1 (MQ=255)
                                      ggTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCgg                                                                                                               >  8:450752/1‑149 (MQ=255)
                                      ggTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGgag                                                                                                             <  6:19735/151‑1 (MQ=255)
                                       gTCAGAATATAGAAGTAGCCCATAAACCCGGTTGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGgaga                                                                                                            <  6:1543860/151‑1 (MQ=255)
                                             atatAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGAt                                                                                                      >  3:91247/1‑151 (MQ=255)
                                               ataGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATc                                                                                                     <  3:600258/150‑1 (MQ=255)
                                               ataGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATc                                                                                                     <  7:413074/150‑1 (MQ=255)
                                               ataGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATAGGATCt                                                                                                    >  7:688627/1‑151 (MQ=255)
                                               ataGAACTAGCCCATACACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGCAGATCGGACCt                                                                                                    <  4:1669709/151‑1 (MQ=255)
                                                 aGAAGTAGCCCATATACCCGGTGGCGTAGAACACGCTGTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCtt                                                                                                   <  4:528064/150‑1 (MQ=255)
                                                   aaGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCa                                                                                                <  2:205959/151‑1 (MQ=255)
                                                    aGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCAc                                                                                               >  2:1060967/1‑151 (MQ=255)
                                                     gTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCAcc                                                                                              >  1:991243/1‑151 (MQ=255)
                                                         cccATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgcc                                                                                          >  7:1411531/1‑151 (MQ=255)
                                                          ccATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCTCCAgccc                                                                                         >  2:49431/1‑151 (MQ=255)
                                                          ccATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgccc                                                                                         >  4:99365/1‑151 (MQ=255)
                                                           cATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAgcccg                                                                                        <  7:1097465/151‑1 (MQ=255)
                                                                aCCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCgg                                                                                   >  6:948221/1‑151 (MQ=255)
                                                                  ccGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCt                                                                                 <  5:877231/151‑1 (MQ=255)
                                                                   cGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCtt                                                                                <  4:1558537/151‑1 (MQ=255)
                                                                   cGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCtt                                                                                >  1:1355703/1‑151 (MQ=255)
                                                                       ggCGTAGAACACGCTCTTACGTGCTTCTCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGAGCGGAGATCGGATCTTTCAACATCCCGCCCGGCTTCatg                                                                            >  6:6350/1‑151 (MQ=255)
                                                                         cGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCatgat                                                                          <  8:1054045/151‑1 (MQ=255)
                                                                                cacGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGCAGCGCATCAGAATGTGCGGCAAGCCCGTCGTCCCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCgccc                                                                   >  2:384979/1‑148 (MQ=255)
                                                                                 acGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGCTCGGATCTTTCACCCGCCCGCCCGGCTTCATGATGTCGacac                                                                  >  2:603045/1‑151 (MQ=255)
                                                                                 acGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGacac                                                                  >  3:1659297/1‑151 (MQ=255)
                                                                                 acGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGacac                                                                  >  5:974460/1‑151 (MQ=255)
                                                                                   gCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCt                                                                >  7:272562/1‑151 (MQ=255)
                                                                                        tACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGCCCCAGAGAGAGCGCGGTGATCGGATCTTTCAGCAGCCGGCCCGTCTTCATGATGTCGACACCTTTCgg                                                           >  6:215969/1‑151 (MQ=255)
                                                                                        tACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCttt                                                              <  3:1085456/148‑1 (MQ=255)
                                                                                         aCGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCggg                                                          >  5:1267100/1‑151 (MQ=255)
                                                                                         aCGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCgg                                                           <  5:1529/150‑1 (MQ=255)
                                                                                          cGTGCTTCGCGGGCAGCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgt                                                         <  2:867246/151‑1 (MQ=255)
                                                                                           gTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgtg                                                        <  7:1314512/151‑1 (MQ=255)
                                                                                            tGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgtgt                                                       >  2:170833/1‑151 (MQ=255)
                                                                                             gCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGgtgt                                                       >  5:471823/1‑150 (MQ=255)
                                                                                                 cgcgGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCg                                                   <  4:1079862/150‑1 (MQ=255)
                                                                                                  gcgGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGcc                                                 <  4:309892/151‑1 (MQ=255)
                                                                                                   cgGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGcc                                                 >  3:1393699/1‑150 (MQ=255)
                                                                                                    gggCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCAt                                               <  6:1217499/151‑1 (MQ=255)
                                                                                                     ggCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATc                                              <  1:45466/151‑1 (MQ=255)
                                                                                                        aTCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGCCCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCt                                           >  8:1617290/1‑151 (MQ=255)
                                                                                                         tCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCtt                                          >  3:1013512/1‑151 (MQ=255)
                                                                                                         gcGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCtt                                          <  2:70528/150‑1 (MQ=255)
                                                                                                          cGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTc                                         >  2:815084/1‑151 (MQ=255)
                                                                                                           gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTc                                         >  1:661253/1‑150 (MQ=255)
                                                                                                           gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTc                                         >  1:311105/1‑150 (MQ=255)
                                                                                                           gCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCa                                        <  8:1581806/151‑1 (MQ=255)
                                                                                                              gACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTg                                     <  5:1496766/151‑1 (MQ=255)
                                                                                                              gACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTg                                     <  5:175285/151‑1 (MQ=255)
                                                                                                              gACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTg                                     <  7:241903/151‑1 (MQ=255)
                                                                                                                cTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGa                                    >  7:1584703/1‑150 (MQ=255)
                                                                                                                  gTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGAGCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAAc                                  <  1:1173700/150‑1 (MQ=255)
                                                                                                                  gTAAACAACCGCATCAGAATGTGCGGCAAGCCCGTAGTACCAAACATCAGTCCCAGACCCAGACAGAGCGCGGAGATCCGATCTTTCACCAGCCCGCCCGGCTTCATGATCTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACa                                 <  8:669858/151‑1 (MQ=255)
                                                                                                                      agaagCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAtt                             >  7:574435/1‑151 (MQ=255)
                                                                                                                        aagCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAttgt                           >  6:111373/1‑151 (MQ=255)
                                                                                                                        aagCGCATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGAttgt                           >  3:1197131/1‑151 (MQ=255)
                                                                                                                           cgcATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGa                        <  5:645639/151‑1 (MQ=255)
                                                                                                                            gcATCAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGaa                       <  3:424150/151‑1 (MQ=255)
                                                                                                                                cAGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCt                    >  8:845458/1‑150 (MQ=255)
                                                                                                                                 aGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTa                   >  6:541208/1‑150 (MQ=255)
                                                                                                                                 aGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTa                   >  5:700776/1‑150 (MQ=255)
                                                                                                                                 aGAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGCTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTa                   >  1:887127/1‑150 (MQ=255)
                                                                                                                                  gAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTaaa                 <  3:503559/151‑1 (MQ=255)
                                                                                                                                  gAATGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTaaa                 <  6:1370085/151‑1 (MQ=255)
                                                                                                                                   aaTGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGATATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAg                <  4:691470/151‑1 (MQ=255)
                                                                                                                                   aaTGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTaa                  >  3:963206/1‑149 (MQ=255)
                                                                                                                                    aTGTGCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGc               <  1:1667706/151‑1 (MQ=255)
                                                                                                                                      gtgCGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGcc              <  3:1570774/150‑1 (MQ=255)
                                                                                                                                         cGGCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACg          >  4:831503/1‑151 (MQ=255)
                                                                                                                                           gCAAGCCCGTCGTACCAAACATCAGTCCCAGACCCTGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACAGCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACgtg        <  6:786850/151‑1 (MQ=255)
                                                                                                                                              aGCCCGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGttt     >  8:476992/1‑151 (MQ=255)
                                                                                                                                              aGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGttt     <  7:843727/151‑1 (MQ=255)
                                                                                                                                                cccGTCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc    >  4:862237/1‑150 (MQ=255)
                                                                                                                                                 ccgtcgTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTtc    >  6:454865/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCACCAACCAGCATGATCGCGCCGAAGCCGATAATAAAGGTCAGAATATAGAAGTAGCCCATAAACCCGGTGGCGTAGAACACGCTCTTACGTGCTTCGCGGGCATCGCTGACTGTAAAGAAGCGCATCAGAATGTGCGGCAAGCCCGCCGTACCAAACATCAGTCCCAGACCCAGAGAGAGCGCGGAGATCGGATCTTTCACCAGCCCGCCCGGCTTCATGATGTCGACACCTTTCGGGTGTACCGCCATCGCTTCACTGAACAGATTGTTGAAGCTAAAGCCGACGTGTTTCAT  >  NC_000913/4283929‑4284224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑