BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,033	C→T	A447T (GCG→ACG)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,033	0	C	T	97.6%	1524.6 / 15.0	456	A447T (GCG→ACG)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (9/2); new base T (235/208); total (244/212)
Fisher's exact test for biased strand distribution p-value = 7.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 457 total aligned reads displayed.

TACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTA  >  NC_000913/4285885‑4286180

tACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCg                                                                                                                                                   >  7:217066/1‑151 (MQ=255)

 aCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCgg                                                                                                                                                  >  7:597129/1‑151 (MQ=255)

   atatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGtt                                                                                                                                                >  1:528837/1‑151 (MQ=255)

      tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGGTGc                                                                                                                                              >  7:896854/1‑150 (MQ=255)

      tttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAAGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGc                                                                                                                                              >  2:158941/1‑150 (MQ=255)

       ttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAACCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCACGCTACCTTCGGTGGCCCCCTCCAGCGGGTTTCCTTCGTTATCGACCAGCGTCGGTTGCAc                                                                                                                                            >  7:77411/1‑151 (MQ=255)

       ttttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCa                                                                                                                                             <  6:62524/150‑1 (MQ=255)

         ttGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGc                                                                                                                                          >  5:537035/1‑151 (MQ=255)

           gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGcc                                                                                                                                         <  8:1345401/150‑1 (MQ=255)

           gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCg                                                                                                                                        <  3:237371/151‑1 (MQ=255)

           gAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTAGTTATCGACCAGCGTCGGTTGCACGCCg                                                                                                                                        >  2:1294929/1‑151 (MQ=255)

           gAAGGTGGAGAAGTAGGTCTGTTCAAAACGATCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCg                                                                                                                                        >  8:870748/1‑151 (MQ=255)

            aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTTGCACGCCg                                                                                                                                        >  4:1488219/1‑150 (MQ=255)

            aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCga                                                                                                                                       <  3:1009735/151‑1 (MQ=255)

            aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCg                                                                                                                                        >  4:249571/1‑150 (MQ=255)

            aaGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCg                                                                                                                                        >  7:503428/1‑150 (MQ=255)

               gTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCgaaga                                                                                                                                    <  2:709604/151‑1 (MQ=255)

                 ggAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCgaagaa                                                                                                                                   <  5:900607/150‑1 (MQ=255)

                     aaGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGAc                                                                                                                              <  1:293863/151‑1 (MQ=255)

                     aaGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGAc                                                                                                                              >  6:299618/1‑151 (MQ=255)

                       gTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACgt                                                                                                                            >  3:1180239/1‑151 (MQ=255)

                         aGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACgtgt                                                                                                                          >  8:203649/1‑151 (MQ=255)

                           gTCTGTTCAAAACGTTCGTGATCGCCAAACTGCGTACGCGCCTGAGCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTGCAGCGGGTTACCTTCGTTATGGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTg                                                                                                                        <  4:1375532/151‑1 (MQ=255)

                              tGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGCCGTGTTGCCg                                                                                                                     >  6:448568/1‑151 (MQ=255)

                              tGTTAAAAACGTTCGTTATCGCCAAACAGAGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCATGCTACCTTATGTGGCCCCCTCCATAGGGTTACCTTCGTTATCGAAAAGCGTCGGTTGCAATCCGAAGCACGGACGTGTTGCCg                                                                                                                     >  4:879391/1‑151 (MQ=255)

                               gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCg                                                                                                                     >  2:847696/1‑150 (MQ=255)

                               gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGa                                                                                                                    <  1:958713/151‑1 (MQ=255)

                                 tCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAc                                                                                                                  >  8:749065/1‑151 (MQ=255)

                                 tCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAc                                                                                                                  >  8:4484/1‑151 (MQ=255)

                                  cAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAcc                                                                                                                 <  5:670250/151‑1 (MQ=255)

                                  cAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAcc                                                                                                                 <  2:203300/151‑1 (MQ=255)

                                     aaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGc                                                                                                              >  3:1667993/1‑151 (MQ=255)

                                     aaCGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGc                                                                                                              >  7:834298/1‑151 (MQ=255)

                                        gTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCttt                                                                                                           >  4:522830/1‑151 (MQ=255)

                                        gTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCttt                                                                                                           <  7:1481722/151‑1 (MQ=255)

                                         ttCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGCCGTGTTGCCGAACCGGCTTTc                                                                                                          >  1:148/1‑151 (MQ=255)

                                          tCGAGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTc                                                                                                          >  6:1561881/1‑150 (MQ=255)

                                           cGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAg                                                                                                        <  4:1560691/151‑1 (MQ=255)

                                             tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGcg                                                                                                      >  5:1058317/1‑150 (MQ=255)

                                             tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCt                                                                                                      >  3:986777/1‑151 (MQ=255)

                                             tGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCt                                                                                                      >  6:566386/1‑151 (MQ=255)

                                              gATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTc                                                                                                     <  5:226696/151‑1 (MQ=255)

                                               aTCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCg                                                                                                    <  8:1134877/151‑1 (MQ=255)

                                                  gCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAAGGGACGTGTTGCCGAACCGGCTTTCAGCTCGGt                                                                                                  >  3:1401020/1‑150 (MQ=255)

                                                  gCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGt                                                                                                  >  8:333194/1‑150 (MQ=255)

                                                  gCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTa                                                                                                 <  1:675211/151‑1 (MQ=255)

                                                    cAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgc                                                                                               >  5:1647411/1‑151 (MQ=255)

                                                     aaaGAGCGTACGCGCCTGACCCGGCCAGGAGGCGGTGATTACCAGGCTACCTTCGGTGGCCCGCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgcg                                                                                              <  5:410537/151‑1 (MQ=255)

                                                      aaCAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAgcgc                                                                                             >  2:288206/1‑151 (MQ=255)

                                                          gCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGCCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAgg                                                                                         <  3:641628/151‑1 (MQ=255)

                                                            gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGCCCCGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGcc                                                                                       >  3:1471102/1‑150 (MQ=255)

                                                            gTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCa                                                                                       <  5:1442351/151‑1 (MQ=255)

                                                              aCGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGc                                                                                     <  7:1363940/151‑1 (MQ=255)

                                                              aCGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGc                                                                                     >  4:1116698/1‑151 (MQ=255)

                                                                gcgcCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCgg                                                                                   >  4:834639/1‑151 (MQ=255)

                                                                gcgcCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCgg                                                                                   <  6:117109/151‑1 (MQ=255)

                                                                      gACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTg                                                                               <  2:518689/149‑1 (MQ=255)

                                                                       aCCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATc                                                                            >  5:801474/1‑151 (MQ=255)

                                                                       aCCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATc                                                                            <  6:381261/151‑1 (MQ=255)

                                                                          cGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATg                                                                         <  4:1290853/151‑1 (MQ=255)

                                                                           ggCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATg                                                                         >  4:277507/1‑150 (MQ=255)

                                                                            gCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGa                                                                        >  1:1566688/1‑150 (MQ=255)

                                                                            gCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGa                                                                        >  6:209248/1‑150 (MQ=255)

                                                                               aGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAcc                                                                    <  2:373372/151‑1 (MQ=255)

                                                                               aGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAcc                                                                    >  6:518395/1‑151 (MQ=255)

                                                                                  aGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAAccgcc                                                                 >  1:857155/1‑151 (MQ=255)

                                                                                     cGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGt                                                              <  6:1216753/151‑1 (MQ=255)

                                                                                        tGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc                                                           >  6:1368121/1‑151 (MQ=255)

                                                                                         gATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc                                                           <  4:475486/150‑1 (MQ=255)

                                                                                         gATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTc                                                           <  3:377855/150‑1 (MQ=255)

                                                                                         gATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCg                                                          <  1:58098/151‑1 (MQ=255)

                                                                                             aCCAGGCTACCTTTGGTGGCCCCCTCGAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCt                                                      <  6:932279/151‑1 (MQ=255)

                                                                                                 ggCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGcca                                                  <  7:55319/151‑1 (MQ=255)

                                                                                                    tACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacc                                                >  2:1082112/1‑150 (MQ=255)

                                                                                                    tACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacc                                                >  1:187562/1‑150 (MQ=255)

                                                                                                     aCCTTCGGTGGCCCCCTCCAGCTGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCCAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacca                                               <  5:305347/150‑1 (MQ=255)

                                                                                                     aCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAg                                              <  6:74147/151‑1 (MQ=255)

                                                                                                     aCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGGCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccacca                                               <  2:1193328/150‑1 (MQ=255)

                                                                                                        ttCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGt                                            >  6:1102395/1‑150 (MQ=255)

                                                                                                          cGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTAt                                          <  7:1614896/150‑1 (MQ=255)

                                                                                                             tGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCCGCTTTCAGCTAGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCCCCCGTTTCGGTCTGCCACCAGGTATCGac                                      >  5:999045/1‑151 (MQ=255)

                                                                                                                cccccTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGaccac                                   <  7:1207072/151‑1 (MQ=255)

                                                                                                                  cccTCCAGCGGGTTACCTTCGTTATCGCCCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAACCCGCCGGTTTCGGTCTGCCACCAGGTATCGACCCCCg                                 >  7:408096/1‑151 (MQ=255)

                                                                                                                  cccTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGACACGAGTTATCGACCACCg                                 <  1:1261506/151‑1 (MQ=255)

                                                                                                                         gCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAttt                          <  4:1677916/151‑1 (MQ=255)

                                                                                                                           gggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGCACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTtct                        >  5:1251078/1‑151 (MQ=255)

                                                                                                                           gggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTtct                        >  8:163411/1‑151 (MQ=255)

                                                                                                                            ggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTtctc                       >  7:1570536/1‑151 (MQ=255)

                                                                                                                               tACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGtt                    >  8:126443/1‑151 (MQ=255)

                                                                                                                               tACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGtt                    >  1:328539/1‑151 (MQ=255)

                                                                                                                                aCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTg                   <  8:1063342/151‑1 (MQ=255)

                                                                                                                                aCCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTg                   <  1:1126976/151‑1 (MQ=255)

                                                                                                                                  cTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGcc                 >  4:787561/1‑151 (MQ=255)

                                                                                                                                    tCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGcc                 <  3:844642/149‑1 (MQ=255)

                                                                                                                                    tCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCg                <  7:294656/150‑1 (MQ=255)

                                                                                                                                      gTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtt             >  5:137008/1‑151 (MQ=255)

                                                                                                                                         aTCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtttt           <  3:1244993/150‑1 (MQ=255)

                                                                                                                                         aTCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAtttt           <  8:142056/150‑1 (MQ=255)

                                                                                                                                           cGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGAttttttt        >  4:589498/1‑151 (MQ=255)

                                                                                                                                               cAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTcc      >  8:670214/1‑149 (MQ=255)

TACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCAGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTA  >  NC_000913/4285885‑4286180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`