Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,303,755 C→T V119I (GTT→ATT)  mdtN ← membrane fusion protein of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,303,7550CT96.4% 1285.4 / 22.2 387V119I (GTT→ATT) mdtNmembrane fusion protein of efflux pump
Reads supporting (aligned to +/- strand):  ref base C (7/7);  new base T (190/181);  total (199/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

AAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAC  >  NC_000913/4303606‑4303902
                                                                                                                                                     |                                                                                                                                                   
  gATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGa                                                                                                                                                  <  2:1163728/151‑1 (MQ=255)
   aTCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCg                                                                                                                                                   >  5:340535/1‑149 (MQ=255)
   aTCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAg                                                                                                                                                 <  4:666916/151‑1 (MQ=255)
    tCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGt                                                                                                                                                >  6:1186681/1‑151 (MQ=255)
     ccGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGtg                                                                                                                                               >  2:1587633/1‑150 (MQ=255)
      cGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCTCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCg                                                                                                                                              <  4:1158141/151‑1 (MQ=255)
      cGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCg                                                                                                                                              >  7:728981/1‑151 (MQ=255)
      cGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCg                                                                                                                                              >  5:62832/1‑151 (MQ=255)
          tCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCa                                                                                                                                           <  7:97664/150‑1 (MQ=255)
          tCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCAc                                                                                                                                          >  3:114603/1‑151 (MQ=255)
                gcgcgcTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACt                                                                                                                                    <  7:1646905/151‑1 (MQ=255)
                  gcgcTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGt                                                                                                                                  >  3:878358/1‑151 (MQ=255)
                    gcTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTg                                                                                                                                >  3:223938/1‑151 (MQ=255)
                       gcgcCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTgcgc                                                                                                                             >  4:357047/1‑151 (MQ=255)
                       gcgcCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTgcgc                                                                                                                             >  8:1278608/1‑151 (MQ=255)
                         gcCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGt                                                                                                                           <  3:1479314/151‑1 (MQ=255)
                            gTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCa                                                                                                                         >  3:1384603/1‑150 (MQ=255)
                             ttCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAac                                                                                                                       >  4:1325405/1‑151 (MQ=255)
                              tCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAac                                                                                                                       <  8:1422562/150‑1 (MQ=255)
                                  gCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAacgcta                                                                                                                   <  1:1393812/150‑1 (MQ=255)
                                   gatggTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAAGGTAGCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAacgctacg                                                                                                                 <  2:574790/148‑1 (MQ=255)
                                    aCGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAacgctacgc                                                                                                                >  8:228857/1‑151 (MQ=255)
                                     cGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAacgctacgct                                                                                                               <  5:670823/151‑1 (MQ=255)
                                     cGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAacgccacgct                                                                                                               <  8:34615/151‑1 (MQ=255)
                                      ggTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTg                                                                                                              <  5:1566428/151‑1 (MQ=255)
                                      ggTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTg                                                                                                              <  1:275587/151‑1 (MQ=255)
                                         cAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGt                                                                                                           <  8:1119731/151‑1 (MQ=255)
                                         cAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGATGCGCGTCAacgctacgctcgct                                                                                                           >  6:211472/1‑147 (MQ=255)
                                                 tCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATa                                                                                                    >  1:385947/1‑150 (MQ=255)
                                                 tCCGCTGAGACAAAACCTTCTTTCAGTAATGGCGCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATa                                                                                                    <  7:1408185/150‑1 (MQ=255)
                                                  ccGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATaa                                                                                                   <  7:1067619/150‑1 (MQ=255)
                                                  ccGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAt                                                                                                  <  8:666213/151‑1 (MQ=255)
                                                    gCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGTGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAttt                                                                                                <  1:611546/151‑1 (MQ=255)
                                                    gCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAAttt                                                                                                >  8:165858/1‑151 (MQ=255)
                                                    gCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGAGCGTCAACGCTACGCTGGGTGAGCATAAttt                                                                                                >  8:157742/1‑151 (MQ=255)
                                                       gagaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTg                                                                                               <  4:936229/149‑1 (MQ=255)
                                                       gagaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                             <  5:745365/151‑1 (MQ=255)
                                                       gagaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCt                                                                                             >  1:913965/1‑151 (MQ=255)
                                                        agaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCtt                                                                                            <  8:1379203/151‑1 (MQ=255)
                                                         gaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTa                                                                                           <  4:1534266/151‑1 (MQ=255)
                                                         gaCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTa                                                                                           <  5:400300/151‑1 (MQ=255)
                                                         caCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTa                                                                                           <  4:1049570/150‑1 (MQ=255)
                                                          ccAAAACCTTCTTTCCGTAATGGCTCGGTGCGGCGTAATGTAGCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTAt                                                                                          <  1:1624196/150‑1 (MQ=255)
                                                          aCAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGGAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTAt                                                                                          >  6:449121/1‑151 (MQ=255)
                                                            aaaaCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTACTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATcc                                                                                        >  7:365125/1‑151 (MQ=255)
                                                               aCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgc                                                                                     <  1:987950/151‑1 (MQ=255)
                                                               aCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgc                                                                                     >  6:91351/1‑151 (MQ=255)
                                                               aCCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGGCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAgc                                                                                     <  6:987384/151‑1 (MQ=255)
                                                                   tcttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccg                                                                                 >  6:675666/1‑151 (MQ=255)
                                                                    cttTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccgc                                                                                >  6:1072981/1‑151 (MQ=255)
                                                                      ttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGcgccg                                                                                 <  3:44250/148‑1 (MQ=255)
                                                                      ttCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCcgcg                                                                               <  3:1660241/150‑1 (MQ=255)
                                                                        cAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgagg                                                                            >  3:35779/1‑151 (MQ=255)
                                                                        cAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgag                                                                             <  1:126880/150‑1 (MQ=255)
                                                                           tAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCgaggg                                                                           >  1:177099/1‑149 (MQ=255)
                                                                            aaTGGCTCGGTGGGGGGTAATGTATCTGTGTCCTGTTTCGCGGCGGCACGGTCTTTTTCTACCGTTGCATTAATCGAGTCGGCACCAAACTGTTGCTCGTCAACGCTAAGCTGGTGGAGCATAATTTGCTTATCCAGCGCCGCtagtgag                                                                         <  4:101627/150‑1 (MQ=255)
                                                                             aTGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGc                                                                       <  5:880317/151‑1 (MQ=255)
                                                                               ggCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCtt                                                                     >  3:1260424/1‑151 (MQ=255)
                                                                               ggCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCtt                                                                     <  6:294452/151‑1 (MQ=255)
                                                                                 cTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTc                                                                    >  3:2761/1‑150 (MQ=255)
                                                                                   cGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGc                                                                 >  1:1025840/1‑151 (MQ=255)
                                                                                      ggcggcgTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCttt                                                              <  7:1502410/150‑1 (MQ=255)
                                                                                      cgcggggTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCttt                                                              >  3:1167026/2‑151 (MQ=255)
                                                                                       gcggcgTACTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTc                                                             <  2:906815/151‑1 (MQ=255)
                                                                                       gcggcgTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTc                                                             <  3:678710/151‑1 (MQ=255)
                                                                                             tAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGcc                                                          >  2:521426/1‑148 (MQ=255)
                                                                                             tAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGcc                                                          >  7:563674/1‑148 (MQ=255)
                                                                                               aTGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGAtt                                                     >  1:54005/1‑151 (MQ=255)
                                                                                                 gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTgg                                                   >  3:1006770/1‑151 (MQ=255)
                                                                                                 gTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTgg                                                   <  3:1113612/151‑1 (MQ=255)
                                                                                                   aTCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCt                                                 >  5:920512/1‑151 (MQ=255)
                                                                                                       gtgGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCtt                                                <  2:1309408/148‑1 (MQ=255)
                                                                                                             tGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGgc                                       >  2:1361589/1‑151 (MQ=255)
                                                                                                             ggtgtCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGgc                                       <  2:1117634/147‑1 (MQ=255)
                                                                                                               gttCGCGGCGGCACGGGCTTTTTCTACCGAGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGgcgc                                     <  6:1679088/150‑1 (MQ=255)
                                                                                                                  ggcggcgGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCggg                                  <  2:400355/150‑1 (MQ=255)
                                                                                                                  cgcgGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCggg                                  <  2:669060/151‑1 (MQ=255)
                                                                                                                   gcggcgGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCgggg                                 >  4:81898/1‑150 (MQ=255)
                                                                                                                   gcggcgGCACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGt                                 >  4:979297/1‑151 (MQ=255)
                                                                                                                    cgccggcACGGGGTTTTTATACCGTGCCATAAATCGAGTCGGCACCAAACTGTTGCCCGTCAACGATACGGTAGGAGAGCATAATTTGCATATCCAGAGCAGGGAGGGAGGCGTCAGCTTTCGACAGATTGGCTTCGTACGGGCGCgggac                                >  6:1303227/4‑149 (MQ=255)
                                                                                                                     ggcggcACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGTGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACAGGCGCGGGTcc                               >  8:515827/1‑150 (MQ=255)
                                                                                                                     ggcggcACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGGGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCg                               <  6:1448995/151‑1 (MQ=255)
                                                                                                                         gcACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGc                           >  7:675668/1‑151 (MQ=255)
                                                                                                                          cACGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCg                          >  4:1456912/1‑151 (MQ=255)
                                                                                                                            cGGGCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCgg                         >  4:583518/1‑150 (MQ=255)
                                                                                                                             gggCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGaa                       <  2:1684910/151‑1 (MQ=255)
                                                                                                                              ggCTTTTTCTAGCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGGGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAAc                      <  4:220587/151‑1 (MQ=255)
                                                                                                                              ggCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAAc                      <  7:1025776/151‑1 (MQ=255)
                                                                                                                               gCTTTTTCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAAc                      >  5:1463172/1‑150 (MQ=255)
                                                                                                                                  ttttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGc                   >  1:849233/1‑150 (MQ=255)
                                                                                                                                  ttttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGATGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGc                   >  1:56739/1‑150 (MQ=255)
                                                                                                                                   tttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGGGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCa                  <  5:126130/150‑1 (MQ=255)
                                                                                                                                    ttCTACCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCaa                 <  7:1306420/150‑1 (MQ=255)
                                                                                                                                        aCCGTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCg             <  5:198827/150‑1 (MQ=255)
                                                                                                                                           gTGGCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGGGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGcccg         >  6:740804/1‑150 (MQ=255)
                                                                                                                                             ggCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTg        <  1:705068/150‑1 (MQ=255)
                                                                                                                                             ggCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGt       <  5:1071169/151‑1 (MQ=255)
                                                                                                                                              gCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt      <  2:301816/151‑1 (MQ=255)
                                                                                                                                              gCATTAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGtt      <  8:843294/151‑1 (MQ=255)
                                                                                                                                                 ttAATCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTg    >  7:847019/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAGATCCGCTTCTGCGGCGCGCTGCGCCGTTCTTGCACGGTCAACATCTTCCGCTGAGACAAAACCTTCTTTCAGTAATGGCTCGGTGCGGCGTAATGTATCTGTGGCCTGTTTCGCGGCGGCACGGGCTTTTTCTACCGTGGCATTAACCGAGTCGGCACCAAACTGTTGCGCGTCAACGCTACGCTGGGTGAGCATAATTTGCTTATCCAGCGCCGCGAGGGAGGCTTCAGCTTTCGCCAGATTGGCTTCGTACGGGCGCGGGTCGATGCGGAACAGCAAATCGCCCTGTTTGAC  >  NC_000913/4303606‑4303902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: