Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,342,825 C→T N305N (AAC→AAT melA → alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,342,8250CT95.5% 1389.6 / 35.2 428N305N (AAC→AATmelAalpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (11/8);  new base T (209/199);  total (221/207)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 428 total aligned reads displayed.

AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGAT  >  NC_000913/4342677‑4342972
                                                                                                                                                    |                                                                                                                                                   
aaaaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAAAGCTGCGTCGAGCAGCTGGCGAATTg                                                                                                                                                   >  1:1444419/1‑151 (MQ=255)
  aaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTgg                                                                                                                                                  >  6:989742/1‑150 (MQ=255)
  aaGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGc                                                                                                                                                 >  5:1527445/1‑151 (MQ=255)
   aGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGc                                                                                                                                                 >  4:825478/1‑150 (MQ=255)
    gCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGATGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCAt                                                                                                                                               <  7:972495/151‑1 (MQ=255)
      tGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATa                                                                                                                                              >  5:31437/1‑150 (MQ=255)
      tGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATa                                                                                                                                              >  1:1513850/1‑150 (MQ=255)
       aggCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATa                                                                                                                                              <  6:1411092/148‑1 (MQ=255)
         gCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATTAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAagc                                                                                                                                          >  8:391703/1‑150 (MQ=255)
            aTTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGc                                                                                                                                        <  6:581840/150‑1 (MQ=255)
            aTTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGGCGAGCAGCTGGCGAATTGGCATaaactgct                                                                                                                                       >  4:1279167/1‑146 (MQ=255)
              ttCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTgg                                                                                                                                     >  2:666910/1‑151 (MQ=255)
                cGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTggag                                                                                                                                   <  2:343076/151‑1 (MQ=255)
                   cACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTggagg                                                                                                                                  >  6:262717/1‑149 (MQ=255)
                   cACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTggagg                                                                                                                                  >  4:1079695/1‑149 (MQ=255)
                   cACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGgaggag                                                                                                                                <  8:231206/151‑1 (MQ=255)
                      ggAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGtat                                                                                                                             <  2:751194/151‑1 (MQ=255)
                      ggAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGtat                                                                                                                             >  8:888796/1‑151 (MQ=255)
                      ggAATCGTCAGAACATTTTCCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGtat                                                                                                                             <  4:901841/151‑1 (MQ=255)
                        aaTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                           <  8:671760/151‑1 (MQ=255)
                         aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaa                                                                                                                          <  2:1260184/151‑1 (MQ=255)
                         aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                           >  1:1589420/1‑150 (MQ=255)
                         aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                           >  7:271027/1‑150 (MQ=255)
                         aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                           >  3:1130486/1‑150 (MQ=255)
                         aTCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGGTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaa                                                                                                                           >  2:1659780/1‑150 (MQ=255)
                          tCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaa                                                                                                                          >  2:543762/1‑150 (MQ=255)
                           cGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaaa                                                                                                                         >  1:633536/1‑150 (MQ=255)
                            gTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGTCATAAAGAGCTGGAGGAGTATaaaaa                                                                                                                        >  7:1116835/1‑150 (MQ=255)
                            gTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATaaaaaa                                                                                                                       <  6:1201503/151‑1 (MQ=255)
                              cAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGc                                                                                                                     <  5:937923/151‑1 (MQ=255)
                                 aaCATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTc                                                                                                                  <  3:395383/151‑1 (MQ=255)
                                   cATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTccc                                                                                                                <  7:1356770/151‑1 (MQ=255)
                                      tttGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCgg                                                                                                              >  8:593174/1‑150 (MQ=255)
                                         gCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGAtt                                                                                                           <  4:1394447/150‑1 (MQ=255)
                                         gCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTg                                                                                                          <  7:1651321/151‑1 (MQ=255)
                                         gCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTg                                                                                                          >  8:1440859/1‑151 (MQ=255)
                                             aGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGatat                                                                                                      <  6:1579590/151‑1 (MQ=255)
                                             aGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGatat                                                                                                      >  3:1018468/1‑151 (MQ=255)
                                              gTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATAtt                                                                                                     <  8:479506/151‑1 (MQ=255)
                                               tACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGGATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATAtt                                                                                                     >  7:758595/1‑150 (MQ=255)
                                                acacacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTa                                                                                                    <  3:1451376/150‑1 (MQ=255)
                                                acacacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGCATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCACCCGCATTGAAATTa                                                                                                    >  4:666556/1‑150 (MQ=255)
                                                 cacacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTaaa                                                                                                  <  6:896579/151‑1 (MQ=255)
                                                  acacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAc                                                                                                 <  1:521263/151‑1 (MQ=255)
                                                  acacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGAACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGACTCACGGATTGATATTaaa                                                                                                  >  7:419028/1‑150 (MQ=255)
                                                   cacCGTTGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAcc                                                                                                <  7:1551595/151‑1 (MQ=255)
                                                   cacCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAAcc                                                                                                >  1:1317772/1‑151 (MQ=255)
                                                             tattaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGa                                                                                       <  1:1643659/150‑1 (MQ=255)
                                                             tattaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGa                                                                                       <  6:431234/150‑1 (MQ=255)
                                                              attaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAat                                                                                     >  8:1188938/1‑151 (MQ=255)
                                                               ttaAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAat                                                                                     >  4:304882/1‑150 (MQ=255)
                                                                taAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAata                                                                                    <  8:1537622/150‑1 (MQ=255)
                                                                taAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAata                                                                                    <  4:183965/150‑1 (MQ=255)
                                                                   gCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGcc                                                                                <  5:1302860/151‑1 (MQ=255)
                                                                   gCCAGGGCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGcc                                                                                <  8:333788/151‑1 (MQ=255)
                                                                    ccAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCCTCGAGCAGCTGGCGAATTGCCATAAAGAGCTGGACGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGccc                                                                               >  2:1362447/1‑150 (MQ=255)
                                                                      aGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGc                                                                             >  5:1011554/1‑151 (MQ=255)
                                                                         tCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGcac                                                                           >  2:1218909/1‑150 (MQ=255)
                                                                          cGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACaa                                                                         <  4:1030994/151‑1 (MQ=255)
                                                                           gTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAAt                                                                        >  8:1679859/1‑151 (MQ=255)
                                                                             gAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATc                                                                       <  5:23802/150‑1 (MQ=255)
                                                                             gAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATc                                                                       >  6:1238199/1‑150 (MQ=255)
                                                                               ggATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATg                                                                    <  3:683185/151‑1 (MQ=255)
                                                                                 aTTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGCGCTGGAGGAGTATAAAACAGCCTCCCGGCTTGATATTAACCCGTCACGGGCATATGCCAGCACACTCATGa                                                                   >  6:103137/1‑150 (MQ=255)
                                                                                 aTTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGa                                                                   >  7:1173479/1‑150 (MQ=255)
                                                                                     gattgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCt                                                              >  1:786698/1‑151 (MQ=255)
                                                                                      attgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCt                                                              >  6:655083/1‑150 (MQ=255)
                                                                                        tgaGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATc                                                           >  5:816187/1‑151 (MQ=255)
                                                                                          aGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAACGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGACCGCTATCTg                                                         >  4:1184383/1‑151 (MQ=255)
                                                                                                ataAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTg                                                    <  6:830889/150‑1 (MQ=255)
                                                                                                  aaaGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCg                                                 >  3:266023/1‑151 (MQ=255)
                                                                                                   aaGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTAGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGa                                                <  6:214782/151‑1 (MQ=255)
                                                                                                       aCCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGcc                                             <  2:117689/150‑1 (MQ=255)
                                                                                                       aCCGCTGGATGAGTACCCGAAACGCTGCGGCGAGCAGCTGGCGAATTGGCATACAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCg                                            <  2:1032240/151‑1 (MQ=255)
                                                                                                         cGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCg                                            >  1:262969/1‑149 (MQ=255)
                                                                                                          gCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAgt                                         <  1:1054700/151‑1 (MQ=255)
                                                                                                           cTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAgtg                                        >  3:1621859/1‑151 (MQ=255)
                                                                                                             ggATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGtgtg                                      <  3:916478/151‑1 (MQ=255)
                                                                                                              gATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGtgtg                                      >  4:458475/1‑150 (MQ=255)
                                                                                                                 gAGTACCCGAAACTCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGAttt                                  <  5:1111474/151‑1 (MQ=255)
                                                                                                                   gTACCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGCGTGATTTAc                                <  5:146613/151‑1 (MQ=255)
                                                                                                                     aCCCGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACg                               <  3:13482/150‑1 (MQ=255)
                                                                                                                      cccGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGc                             <  4:1522890/151‑1 (MQ=255)
                                                                                                                      cccGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGc                             <  4:407788/151‑1 (MQ=255)
                                                                                                                       ccGAATCTCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACggga                            <  6:1103872/151‑3 (MQ=255)
                                                                                                                       ccGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCa                            <  5:1654848/151‑1 (MQ=255)
                                                                                                                       ccGAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGAGTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGc                             >  2:1516003/1‑150 (MQ=255)
                                                                                                                         gAAACGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAAc                          <  5:1215324/151‑1 (MQ=255)
                                                                                                                          aaaCGCTGCGTCGAGCAGCTGGCGAATTGGCATAAAGAGCTGGTCGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACg                         <  2:917630/151‑1 (MQ=255)
                                                                                                                                     cGAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAATCCGTCACGGGAATATGCCAGCACAATCATGACCGCTATCTGGCCTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGAt              >  4:589647/1‑151 (MQ=255)
                                                                                                                                      gAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATg             <  7:823020/151‑1 (MQ=255)
                                                                                                                                      gAGCAGCTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATg             <  8:1277361/151‑1 (MQ=255)
                                                                                                                                       agcagcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATgg            >  6:1334042/1‑151 (MQ=255)
                                                                                                                                         cagcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGtt          <  4:1150501/151‑1 (MQ=255)
                                                                                                                                          agcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGttt         >  5:50905/1‑151 (MQ=255)
                                                                                                                                          agcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGttt         >  1:1143143/1‑151 (MQ=255)
                                                                                                                                           gcTGGCGAATTGGCATAAAGCGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttg        <  7:52745/151‑1 (MQ=255)
                                                                                                                                           gcTGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGGCCGTAACGATGGTttg        <  5:595329/151‑1 (MQ=255)
                                                                                                                                            gtGGCGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttga       <  3:1284642/150‑1 (MQ=255)
                                                                                                                                                cGAATTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTttgattga   <  3:494437/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAAAGCTGGGCTATTTCGTCACGGAATCGTCAGAACATTTTGCTGAGTACACACCGTGGTTTATTAAGCCAGGTCGTGAGGATTTGATTGAGCGTTATAAAGTACCGCTGGATGAGTACCCGAAACGCTGCGTCGAGCAGCTGGCGAACTGGCATAAAGAGCTGGAGGAGTATAAAAAAGCCTCCCGGATTGATATTAAACCGTCACGGGAATATGCCAGCACAATCATGAACGCTATCTGGACTGGCGAGCCGAGTGTGATTTACGGCAACGTCCGTAACGATGGTTTGATTGAT  >  NC_000913/4342677‑4342972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: