Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,351,590 C→T V25I (GTC→ATC)  dcuS ← sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,351,5900CT97.6% 1584.6 / 15.7 464V25I (GTC→ATC) dcuSsensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base T (215/237);  total (219/245)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 464 total aligned reads displayed.

GCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATT  >  NC_000913/4351443‑4351737
                                                                                                                                                   |                                                                                                                                                   
gCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCa                                                                                                                                                   <  2:1452147/150‑1 (MQ=255)
gCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCAt                                                                                                                                                  >  4:834339/1‑151 (MQ=255)
 cAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCAtt                                                                                                                                                 >  6:141160/1‑151 (MQ=255)
  aaGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTa                                                                                                                                                <  1:1047695/151‑1 (MQ=255)
  aaGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTa                                                                                                                                                <  5:603983/151‑1 (MQ=255)
  aaGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTa                                                                                                                                                <  4:1310571/151‑1 (MQ=255)
          aCGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATGACt                                                                                                                                        >  6:1112750/1‑151 (MQ=255)
           cGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtg                                                                                                                                       >  5:1421984/1‑151 (MQ=255)
            ggATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtgt                                                                                                                                      >  7:1643803/1‑151 (MQ=255)
             gATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACtgtg                                                                                                                                     >  8:536017/1‑151 (MQ=255)
              aTTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTgg                                                                                                                                    >  3:191492/1‑151 (MQ=255)
                 tCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTa                                                                                                                                  <  2:1235115/150‑1 (MQ=255)
                 tCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTAc                                                                                                                                 >  2:1284632/1‑151 (MQ=255)
                   cGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTc                                                                                                                               >  4:221061/1‑151 (MQ=255)
                      cGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAAt                                                                                                                            <  3:892005/151‑1 (MQ=255)
                       gAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAAtt                                                                                                                           <  8:1260847/151‑1 (MQ=255)
                        aGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAAttt                                                                                                                          <  7:1071577/151‑1 (MQ=255)
                           cGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCAt                                                                                                                       >  6:434959/1‑151 (MQ=255)
                            ggCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATc                                                                                                                      <  4:1011768/151‑1 (MQ=255)
                            ggCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATc                                                                                                                      <  8:1489466/151‑1 (MQ=255)
                               gAGGGTACGCGCCACTGCCAGTGCCTTGTTGGGTAGCACATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCCCCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCCATTTCATCggc                                                                                                                   >  8:765395/1‑150 (MQ=255)
                                aGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGAc                                                                                                                  <  4:450906/151‑1 (MQ=255)
                                aGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGAc                                                                                                                  <  7:1522599/151‑1 (MQ=255)
                                  ggTACGCGCCACTGCCAGTGCCTTGTTGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGtt                                                                                                               <  6:1166847/151‑1 (MQ=255)
                                     aCGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCCCCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTg                                                                                                             >  3:117873/1‑151 (MQ=255)
                                       gcgcCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCg                                                                                                           <  8:195096/151‑1 (MQ=255)
                                           cACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAAc                                                                                                       >  3:971077/1‑151 (MQ=255)
                                             cTGCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACAt                                                                                                     >  1:1004833/1‑151 (MQ=255)
                                               gCCAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGc                                                                                                   <  8:389251/151‑1 (MQ=255)
                                                ccAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCg                                                                                                  <  6:244315/151‑1 (MQ=255)
                                                 cAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCgg                                                                                                 <  3:1606679/151‑1 (MQ=255)
                                                 cAGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCgg                                                                                                 >  5:443650/1‑151 (MQ=255)
                                                  aGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGt                                                                                                >  8:616809/1‑151 (MQ=255)
                                                  aGTGCCTTGTTGGCTAGCCCATCTCGTGTCATATAACTGTTTTGCGATAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCATTTTCATCTGACGTTTGCGTAACATGCGGt                                                                                                <  1:1399307/151‑1 (MQ=255)
                                                     gCCTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCAGCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTGAATTTCATCGGACGTTTGCGTAACATGCGGTAgg                                                                                             <  3:613734/151‑1 (MQ=255)
                                                     gCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAgg                                                                                             >  3:392478/1‑151 (MQ=255)
                                                       cTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGc                                                                                           >  3:1478480/1‑151 (MQ=255)
                                                       cTTGTTGGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGc                                                                                           >  5:93298/1‑151 (MQ=255)
                                                         tgttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCa                                                                                          <  2:1081581/150‑1 (MQ=255)
                                                          gttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCaa                                                                                         <  5:1246024/150‑1 (MQ=255)
                                                           ttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCaatg                                                                                       >  5:801881/1‑151 (MQ=255)
                                                           ttgGCTAGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCaatg                                                                                       >  5:1538408/1‑151 (MQ=255)
                                                                 atcccATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGt                                                                                  <  3:576445/148‑1 (MQ=255)
                                                                 aGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATGGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGtc                                                                                 <  2:447743/151‑1 (MQ=255)
                                                                 aGCCCATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCaatgaatg                                                                                   <  2:774154/149‑1 (MQ=255)
                                                                  gCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGtct                                                                                <  8:1637797/151‑1 (MQ=255)
                                                                   cccATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGtctc                                                                               >  7:203751/1‑151 (MQ=255)
                                                                     cATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCtcat                                                                             <  1:762182/151‑1 (MQ=255)
                                                                     cATCTCGTGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCtcat                                                                             >  8:1586919/1‑151 (MQ=255)
                                                                        ctcGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCtcatc                                                                            >  8:474675/1‑149 (MQ=255)
                                                                          cgtgTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCANCGGACGTTTGNGTAACATGCGGTAGGGCAATGAATGTCtcatc                                                                            <  5:16288/144‑1 (MQ=255)
                                                                                 tatCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgt                                                                 >  3:1318413/1‑151 (MQ=255)
                                                                                   tCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgtgt                                                               >  3:255656/1‑151 (MQ=255)
                                                                                    cACTGATTTGCTAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgtgt                                                               <  4:1582654/150‑1 (MQ=255)
                                                                                    cACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAAGAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTtgtgtg                                                              <  7:79368/151‑1 (MQ=255)
                                                                                        gATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACa                                                           <  2:84313/150‑1 (MQ=255)
                                                                                         aTTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACaaa                                                         <  3:471169/151‑1 (MQ=255)
                                                                                         aTTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACaa                                                          >  6:1551582/1‑150 (MQ=255)
                                                                                           ttGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAAtt                                                       >  1:1610711/1‑151 (MQ=255)
                                                                                            tGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAAtt                                                       <  2:961858/150‑1 (MQ=255)
                                                                                             gCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTc                                                     <  1:455694/151‑1 (MQ=255)
                                                                                               gagaAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCtt                                                   >  5:887354/1‑151 (MQ=255)
                                                                                               gagaAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCtt                                                   <  5:231713/151‑1 (MQ=255)
                                                                                               cagaAGTAAATAACATGCACCACCAATAGCACAGATAACAGTACCGCACTGATCATTAAGATCACTGAGGTACTCAATTTCATCGGACTTTTGCGTAACATGCGGTAGGGCAATGATTGTCTCATCAGCTTCCTTGTATGAAAAAATTCtt                                                   >  2:658483/2‑151 (MQ=255)
                                                                                                agaAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTa                                                  <  4:388967/151‑1 (MQ=255)
                                                                                                 gaAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTaa                                                 <  7:469843/151‑1 (MQ=255)
                                                                                                   aGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGc                                               >  3:683010/1‑151 (MQ=255)
                                                                                                      aaaTCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCAtt                                            >  6:844487/1‑151 (MQ=255)
                                                                                                      aaaTCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCAt                                             <  5:277350/150‑1 (MQ=255)
                                                                                                       aaTCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTa                                           <  1:546791/151‑1 (MQ=255)
                                                                                                         tCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTAt                                          >  4:1442205/1‑150 (MQ=255)
                                                                                                          cAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTAtc                                         <  8:1059127/150‑1 (MQ=255)
                                                                                                          aaGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTAtct                                        <  1:911171/150‑1 (MQ=255)
                                                                                                            gATGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGCACCTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATctct                                      >  2:1627870/1‑151 (MQ=255)
                                                                                                             aTGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCtg                                     <  1:604359/151‑1 (MQ=255)
                                                                                                             aTGCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCtg                                     >  4:967139/1‑151 (MQ=255)
                                                                                                               gCACCACCAATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCtga                                    <  1:284379/150‑1 (MQ=255)
                                                                                                                    accaATAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGc                              <  8:350529/151‑1 (MQ=255)
                                                                                                                        aTAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGt                          <  1:38884/151‑1 (MQ=255)
                                                                                                                         tAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGt                          >  3:320798/1‑150 (MQ=255)
                                                                                                                         tAGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTAATCTGACGTTTTAGTAACATGCTGTAGGTAAATGAATGTCTAATAAGCTTCCTTGTTTAACAAATTTCTGAAGCATTATCTATCATGAGGAGGGt                          >  6:665362/1‑150 (MQ=255)
                                                                                                                          aGCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTa                         <  1:1540987/150‑1 (MQ=255)
                                                                                                                           gCACCGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAAt                       <  7:360639/151‑1 (MQ=255)
                                                                                                                            cACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAAtt                      <  7:1267669/151‑1 (MQ=255)
                                                                                                                              ccGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCa                    <  6:994960/151‑1 (MQ=255)
                                                                                                                               cGAGAACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCa                    >  8:1160100/1‑150 (MQ=255)
                                                                                                                                 agaACAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAg                 <  5:1043959/151‑1 (MQ=255)
                                                                                                                                   aaCAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAggg               <  1:1477701/151‑1 (MQ=255)
                                                                                                                                    aCAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAggg               >  1:123292/1‑150 (MQ=255)
                                                                                                                                    aCAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGa              <  7:898995/151‑1 (MQ=255)
                                                                                                                                     cAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAggg               >  2:1548821/1‑149 (MQ=255)
                                                                                                                                     cAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAggg               >  7:694558/1‑149 (MQ=255)
                                                                                                                                     cAGTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGAGTAATTCAAAGGGAg             <  7:1390124/151‑1 (MQ=255)
                                                                                                                                       gTACCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTa           <  4:1146134/151‑1 (MQ=255)
                                                                                                                                         aCCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTaa          <  6:536583/150‑1 (MQ=255)
                                                                                                                                         aCCGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTaa          <  2:194019/150‑1 (MQ=255)
                                                                                                                                           cGCACTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGTGTAATTCAAAGGGAGTAAGaa       <  8:1314372/151‑1 (MQ=255)
                                                                                                                                              aCTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATg     >  1:97772/1‑150 (MQ=255)
                                                                                                                                              aCTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGa    <  7:284579/151‑1 (MQ=255)
                                                                                                                                               cTGATCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGAt   >  6:1657708/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCAAGCCCTGACGGATTTCCGGCGAGTCGGCGAGGGTACGCGCCACTGCCAGTGCCTTGTTGGCTAGCCCATCTCGCGTCATATCACTGATTTGCGAGAAGTAAATCAGATGCACCACCAATAGCACCGAGAACAGTACCGCACTGACCATTAAGATCACTGTGGTACTCAATTTCATCGGACGTTTGCGTAACATGCGGTAGGGCAATGAATGTCTCATCAGCTTCCTTGTGTGACAAATTTCTTAAGCATTATCTCTGATGAGGCGGGTAATTCAAAGGGAGTAAGAATGATT  >  NC_000913/4351443‑4351737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: