BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,421	C→T	A272T (GCG→ACG)	yjeJ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,421	0	C	T	96.9%	1497.2 / 23.0	448	A272T (GCG→ACG)	yjeJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (8/6); new base T (217/216); total (226/222)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 458 total aligned reads displayed.

AAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGG  >  NC_000913/4373273‑4373568

aaGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgtgc                                                                                                                                                   >  4:1660530/1‑151 (MQ=255)

aaGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgtgc                                                                                                                                                   <  7:480560/151‑1 (MQ=255)

  ggCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAgcgtgcgt                                                                                                                                                 <  6:780784/151‑1 (MQ=255)

    cGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTa                                                                                                                                                >  7:176299/1‑150 (MQ=255)

     gTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTa                                                                                                                                                >  8:611576/1‑149 (MQ=255)

      tttACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGc                                                                                                                                             >  7:394201/1‑151 (MQ=255)

         actccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTGAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAt                                                                                                                                           <  4:527696/147‑1 (MQ=255)

         aCGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCAtt                                                                                                                                          >  2:1309328/1‑151 (MQ=255)

           gccgcATCCGGCATGACCAACGATCACGTTATCAGTCATTTAAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTg                                                                                                                                         >  5:855816/1‑150 (MQ=255)

              gcATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATcc                                                                                                                                     >  8:293714/1‑151 (MQ=255)

               cATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCt                                                                                                                                    >  3:97110/1‑151 (MQ=255)

               cATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCt                                                                                                                                    <  6:633489/151‑1 (MQ=255)

                  ccGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTggg                                                                                                                                 >  5:1141334/1‑151 (MQ=255)

                   cGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGt                                                                                                                                >  6:557024/1‑151 (MQ=255)

                     gCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTa                                                                                                                              <  6:828934/151‑1 (MQ=255)

                      cATGACCAGCGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAg                                                                                                                             <  6:163730/151‑1 (MQ=255)

                         gACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCg                                                                                                                           <  8:1704503/150‑1 (MQ=255)

                         gACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCg                                                                                                                           <  5:1611453/150‑1 (MQ=255)

                          aCCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGt                                                                                                                         <  3:612798/151‑1 (MQ=255)

                           ccAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGtt                                                                                                                        >  3:806440/1‑151 (MQ=255)

                            cAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTg                                                                                                                       <  2:1236695/151‑1 (MQ=255)

                             aaCGATCTCGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTgg                                                                                                                      <  8:1540546/151‑1 (MQ=255)

                             aaCGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTgg                                                                                                                      >  1:1231310/1‑151 (MQ=255)

                              aCGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTggg                                                                                                                     >  8:881661/1‑151 (MQ=255)

                                gATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCg                                                                                                                   >  5:516857/1‑151 (MQ=255)

                                  tCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGtt                                                                                                                 >  3:256873/1‑151 (MQ=255)

                                   cACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTa                                                                                                                <  6:146979/151‑1 (MQ=255)

                                   cACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTa                                                                                                                <  3:422124/151‑1 (MQ=255)

                                        tATCAGTCATTTCAGGCTGGATGGCTCCGGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcg                                                                                                            <  5:1510367/150‑1 (MQ=255)

                                        tATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgc                                                                                                           >  1:888683/1‑151 (MQ=255)

                                        tATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcg                                                                                                            <  1:347116/150‑1 (MQ=255)

                                         aTCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgc                                                                                                           <  8:527845/150‑1 (MQ=255)

                                          tCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgccg                                                                                                         >  6:893161/1‑151 (MQ=255)

                                           cAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTcgccgc                                                                                                        >  5:674013/1‑151 (MQ=255)

                                            aGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCt                                                                                                       <  2:195998/151‑1 (MQ=255)

                                            aGTAATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCt                                                                                                       <  7:977944/151‑1 (MQ=255)

                                              tCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTTGCGGTTGGGCGTTATTCGCCGCTAc                                                                                                     >  6:34412/1‑151 (MQ=255)

                                              tCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTAc                                                                                                     >  1:197003/1‑151 (MQ=255)

                                               cATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTAc                                                                                                     <  1:100143/150‑1 (MQ=255)

                                                aTTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGATCACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACgg                                                                                                   >  4:1555188/1‑151 (MQ=255)

                                                aTTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACgg                                                                                                   >  1:679621/1‑151 (MQ=255)

                                                    cAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGc                                                                                                <  6:139905/150‑1 (MQ=255)

                                                    cAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCg                                                                                               >  7:1095512/1‑151 (MQ=255)

                                                     agccTGGATGGCTCAAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGAGCTGCAGTTTTGCTGCTGGATTGAACACCTAAGTGATGCAGCGAGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGCTg                                                                                                 >  4:808850/4‑148 (MQ=255)

                                                     aGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCg                                                                                               >  7:1433617/1‑150 (MQ=255)

                                                     aGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGa                                                                                              <  7:1270622/151‑1 (MQ=255)

                                                         tGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACAt                                                                                          >  7:1533767/1‑151 (MQ=255)

                                                          ggATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACa                                                                                           <  1:159636/149‑1 (MQ=255)

                                                           gATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAg                                                                                        <  5:423988/151‑1 (MQ=255)

                                                                  ccAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGTGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGAc                                                                                 >  6:1308213/1‑151 (MQ=255)

                                                                   cAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACa                                                                                <  2:67231/151‑1 (MQ=255)

                                                                      ccTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATgg                                                                             >  1:972888/1‑151 (MQ=255)

                                                                       cTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGc                                                                            >  5:1040245/1‑151 (MQ=255)

                                                                          gCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGcac                                                                          >  2:376964/1‑150 (MQ=255)

                                                                           cATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCacac                                                                        >  6:1062636/1‑151 (MQ=255)

                                                                            aTTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACAcc                                                                       >  8:645656/1‑151 (MQ=255)

                                                                              tAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCcg                                                                     >  2:560312/1‑151 (MQ=255)

                                                                                 cAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCcgcg                                                                   >  5:769083/1‑150 (MQ=255)

                                                                                      aTGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGtttt                                                             <  5:241527/151‑1 (MQ=255)

                                                                                         agaTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTc                                                          <  1:44818/151‑1 (MQ=255)

                                                                                          gaTTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCa                                                         <  7:627853/151‑1 (MQ=255)

                                                                                                 tCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTg                                                   <  8:1203592/150‑1 (MQ=255)

                                                                                                 tCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTg                                                   <  8:1104258/150‑1 (MQ=255)

                                                                                                  cGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTggg                                                 >  3:523519/1‑151 (MQ=255)

                                                                                                    ccTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCt                                               <  1:739409/151‑1 (MQ=255)

                                                                                                      tgtgGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTg                                              >  2:814253/1‑150 (MQ=255)

                                                                                                        tggtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGaa                                            >  1:1032791/1‑150 (MQ=255)

                                                                                                         ggtgCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGaaa                                           <  5:124621/150‑1 (MQ=255)

                                                                                                            gCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCa                                        >  2:1039839/1‑150 (MQ=255)

                                                                                                              aGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGc                                     <  8:1668579/151‑1 (MQ=255)

                                                                                                               gTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCa                                    <  6:1183653/151‑1 (MQ=255)

                                                                                                                 tttGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCAtt                                  >  3:896509/1‑151 (MQ=255)

                                                                                                                 tttGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCAtt                                  >  6:265924/1‑151 (MQ=255)

                                                                                                                 tttGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTGGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCAt                                   <  4:702042/150‑1 (MQ=255)

                                                                                                                  ttGCTGGTGGATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACAGCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTc                                 >  4:1086010/1‑151 (MQ=255)

                                                                                                                      tggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCg                             >  4:959364/1‑151 (MQ=255)

                                                                                                                       ggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGc                              <  1:1530184/149‑1 (MQ=255)

                                                                                                                       ggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCg                             <  7:205657/150‑1 (MQ=255)

                                                                                                                       ggtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGa                            <  8:1264307/151‑1 (MQ=255)

                                                                                                                        gtggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAg                           <  2:926884/151‑1 (MQ=255)

                                                                                                                         tggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGt                          >  3:1244331/1‑151 (MQ=255)

                                                                                                                          ggATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTg                         <  1:1177075/151‑1 (MQ=255)

                                                                                                                           gATTGAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGa                        <  3:1531980/151‑1 (MQ=255)

                                                                                                                               gAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCg                     <  8:811264/150‑1 (MQ=255)

                                                                                                                               gAACACGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGc                    >  5:726643/1‑151 (MQ=255)

                                                                                                                                 acacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCtt                  <  3:1301299/151‑1 (MQ=255)

                                                                                                                                 acacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCtt                  >  3:1556232/1‑151 (MQ=255)

                                                                                                                                  cacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTc                 >  7:786013/1‑151 (MQ=255)

                                                                                                                                  cacGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTc                 >  5:925802/1‑151 (MQ=255)

                                                                                                                                   acGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCt                >  8:1437741/1‑151 (MQ=255)

                                                                                                                                    cGTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTGCGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGCGTGATCGCTTCt                >  2:1509954/1‑150 (MQ=255)

                                                                                                                                     gTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAt              <  6:992647/151‑1 (MQ=255)

                                                                                                                                     gTAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAt              >  5:1375932/1‑151 (MQ=255)

                                                                                                                                      tAAGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTAtt             >  4:1306247/1‑151 (MQ=255)

                                                                                                                                        aGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTc           <  3:1661937/151‑1 (MQ=255)

                                                                                                                                        aGTGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTc           <  5:310303/151‑1 (MQ=255)

                                                                                                                                          tGATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCtt         >  3:1444322/1‑151 (MQ=255)

                                                                                                                                           gATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCttt        >  4:1444532/1‑151 (MQ=255)

                                                                                                                                           gATGCAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTAGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCtt         <  4:246118/150‑1 (MQ=255)

                                                                                                                                               cAGCGTGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTAccc    <  7:11828/151‑1 (MQ=255)

AAGGCGTTTACGCCGCATCCGGCATGACCAACGATCACGTTATCAGTCATTTCAGGCTGGATGGCTCCAGCCTCGCATTAGCAAAGATGAGATTATTTCGCCTGTGGTGCAGTTTTGCTGGTGGATTGAACACGTAAGTGATGCAGCGCGCGTAAGCATTGATCCTGGGTTAGCGGTTGGGCGTTATTCGCCGCTACGGTGCGAACATAGACCTGACATGGCACACCCGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGG  >  NC_000913/4373273‑4373568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`