Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 306,023 G→A T265T (ACC→ACT)  ecpD ← polymerized tip adhesin of ECP fibers

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913306,0230GA95.4% 1414.2 / 37.0 432T265T (ACC→ACT) ecpDpolymerized tip adhesin of ECP fibers
Reads supporting (aligned to +/- strand):  ref base G (9/11);  new base A (196/216);  total (205/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 432 total aligned reads displayed.

AGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGATTTG  >  NC_000913/305875‑306171
                                                                                                                                                    |                                                                                                                                                    
agtaATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGt                                                                                                                                                    <  2:1276333/151‑1 (MQ=255)
   aaTAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGtata                                                                                                                                                 <  6:458210/151‑1 (MQ=255)
   aaTAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGtat                                                                                                                                                  >  1:239455/1‑150 (MQ=255)
      aGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                              <  6:393826/151‑1 (MQ=255)
      aGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                              <  5:1265297/151‑1 (MQ=255)
       ggCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGtt                                                                                                                                              >  7:1049936/1‑150 (MQ=255)
        ggggTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGATTGCAGAGTATAGTTaa                                                                                                                                            <  2:601425/149‑1 (MQ=255)
         cGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAc                                                                                                                                           >  6:1216372/1‑151 (MQ=255)
         cGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGGCCGTTTGTTTGCAGAGTATAGTTAAc                                                                                                                                           <  1:1527113/151‑1 (MQ=255)
           gTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAcc                                                                                                                                          >  6:287318/1‑150 (MQ=255)
           gTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGAAGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAcc                                                                                                                                          >  7:674358/1‑150 (MQ=255)
               tGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCtt                                                                                                                                     >  5:1646696/1‑151 (MQ=255)
                gCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCtt                                                                                                                                     >  8:1341697/1‑150 (MQ=255)
                  tCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTAc                                                                                                                                   >  2:241841/1‑150 (MQ=255)
                  tCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTAc                                                                                                                                   >  8:229460/1‑150 (MQ=255)
                    aCCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGCCGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAACATCTTACAAc                                                                                                                                >  4:700234/1‑151 (MQ=255)
                    aCCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAAc                                                                                                                                >  7:1613348/1‑151 (MQ=255)
                     ccGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACt                                                                                                                               >  2:1141922/1‑151 (MQ=255)
                      cGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACtt                                                                                                                              >  7:469364/1‑151 (MQ=255)
                        gTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTa                                                                                                                             >  2:390923/1‑150 (MQ=255)
                         tttCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTCAAt                                                                                                                           >  3:406650/1‑151 (MQ=255)
                          ttCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAAt                                                                                                                           <  8:1655979/150‑1 (MQ=255)
                            ccATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCt                                                                                                                        <  2:697805/151‑1 (MQ=255)
                              aTGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGa                                                                                                                      <  6:809423/151‑1 (MQ=255)
                               tGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGa                                                                                                                      >  3:888333/1‑150 (MQ=255)
                                gAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGaa                                                                                                                     <  1:1148785/150‑1 (MQ=255)
                                      ccATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGc                                                                                                              >  5:758918/1‑151 (MQ=255)
                                       cATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGcc                                                                                                             <  4:46769/151‑1 (MQ=255)
                                           cAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATg                                                                                                         >  4:835265/1‑151 (MQ=255)
                                           cAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATg                                                                                                         <  5:394197/151‑1 (MQ=255)
                                                  aaCTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCg                                                                                                  <  2:1626951/151‑1 (MQ=255)
                                                   aCTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGt                                                                                                 >  1:525295/1‑151 (MQ=255)
                                                    cTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTc                                                                                                >  2:1355870/1‑151 (MQ=255)
                                                    cTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTc                                                                                                >  3:1637416/1‑151 (MQ=255)
                                                     tGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTcc                                                                                               >  3:58616/1‑151 (MQ=255)
                                                       cATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCgg                                                                                             <  1:797152/151‑1 (MQ=255)
                                                         tatCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGc                                                                                            >  5:316168/1‑150 (MQ=255)
                                                          atCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGc                                                                                            >  1:340302/1‑149 (MQ=255)
                                                          atCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGc                                                                                            >  7:1029513/1‑149 (MQ=255)
                                                             gTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCg                                                                                       <  4:1312316/151‑1 (MQ=255)
                                                             gTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTCTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCg                                                                                       >  3:1452041/1‑151 (MQ=255)
                                                              tACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCgg                                                                                      >  3:1280160/1‑151 (MQ=255)
                                                                 gCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGt                                                                                   >  3:721730/1‑151 (MQ=255)
                                                                  ccccGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGtt                                                                                  <  8:375208/151‑1 (MQ=255)
                                                                     cGACGGCCGTGGCTAACCAGGCGGTGGCGATCGCCGGGAATATATGGATTGTGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTCCCGATGGTTTGCGTCCGGCAGTCGGCGTTccc                                                                               <  4:29502/151‑1 (MQ=255)
                                                                     cGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTcc                                                                                >  6:896157/1‑150 (MQ=255)
                                                                        cGGCCGAGGCTAACGTCGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTc                                                                            <  2:1440926/151‑1 (MQ=255)
                                                                             gAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCa                                                                       <  7:970710/151‑1 (MQ=255)
                                                                             gAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCa                                                                       <  5:112981/151‑1 (MQ=255)
                                                                                   aaCGACTAGTTGGCGAGCGCCGGGAATATATGGATTGAGGGGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTc                                                                  <  2:52358/150‑1 (MQ=255)
                                                                                          aGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTcc                                                          <  5:1408881/151‑1 (MQ=255)
                                                                                           cttGGCGATCGACGGGAATATATGGATTGAGGTGATGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAAGCATCTTACAACTTAATCCTGAACGGATGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCACCCAGAGTCGGTACTCCg                                                         >  2:78317/2‑151 (MQ=255)
                                                                                            ttGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGt                                                        >  5:1557471/1‑151 (MQ=255)
                                                                                              ggCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTc                                                       <  7:603631/150‑1 (MQ=255)
                                                                                                cGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCt                                                    <  5:489928/151‑1 (MQ=255)
                                                                                                 gATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTg                                                   >  1:496384/1‑151 (MQ=255)
                                                                                                 gATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTg                                                   >  3:260470/1‑151 (MQ=255)
                                                                                                    cggcGGGAATATATGGTTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGACGGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGtt                                                 <  3:1543995/147‑1 (MQ=255)
                                                                                                    cGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                >  7:816449/1‑151 (MQ=255)
                                                                                                    cGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTg                                                <  4:1031231/151‑1 (MQ=255)
                                                                                                       cGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATa                                             <  8:694832/151‑1 (MQ=255)
                                                                                                         ggAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaaa                                           <  5:122957/151‑1 (MQ=255)
                                                                                                         ggAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaaa                                           <  1:1084616/151‑1 (MQ=255)
                                                                                                         ggAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATaa                                            >  1:723456/1‑150 (MQ=255)
                                                                                                          gAATATATGGATTGAGGTGTTGCTGAGTCCGTTTCTTTGCACAGTATAGTTAACCATCTTACAACTTAATCCTGAACCGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGACACGGTACTCCGTCGCTGTTGATAAAt                                          >  7:253086/1‑151 (MQ=255)
                                                                                                            atatatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATAc                                        >  5:171569/1‑151 (MQ=255)
                                                                                                            atatatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATAc                                        >  8:553181/1‑151 (MQ=255)
                                                                                                            atatatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATAc                                        <  7:379599/151‑1 (MQ=255)
                                                                                                             tatatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACt                                       >  7:1268264/1‑151 (MQ=255)
                                                                                                             tatatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACt                                       >  2:168309/1‑151 (MQ=255)
                                                                                                              atatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACt                                       <  4:1664949/150‑1 (MQ=255)
                                                                                                              atatGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACt                                       <  2:933772/150‑1 (MQ=255)
                                                                                                                atGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGTTAAATACTTc                                     <  1:1313502/150‑1 (MQ=255)
                                                                                                                atGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTcc                                    >  4:391477/1‑151 (MQ=255)
                                                                                                                  ggATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCg                                   <  4:1567679/150‑1 (MQ=255)
                                                                                                                    aTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGcc                                 >  8:122131/1‑150 (MQ=255)
                                                                                                                    aTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGcc                                 >  2:2980/1‑150 (MQ=255)
                                                                                                                    aTTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGGCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCa                                <  6:1344049/151‑1 (MQ=255)
                                                                                                                      tGAGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAg                               <  4:878196/150‑1 (MQ=255)
                                                                                                                       gAGGTGTTGCTGAGTCCGTTTGGTTGCAGAGTATAGTTACCCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCg                             <  3:825199/151‑1 (MQ=255)
                                                                                                                        aGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGa                            <  1:1553352/151‑1 (MQ=255)
                                                                                                                        aGGTGTTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGa                            <  7:1221814/151‑1 (MQ=255)
                                                                                                                            gtTGCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTgg                        <  4:98858/151‑1 (MQ=255)
                                                                                                                               gCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGt                      <  2:429206/150‑1 (MQ=255)
                                                                                                                               gCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGt                      <  1:953463/150‑1 (MQ=255)
                                                                                                                               gCTGAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTa                     <  6:601404/151‑1 (MQ=255)
                                                                                                                                  gAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTAttt                  <  8:1524034/151‑1 (MQ=255)
                                                                                                                                  gAGTCCGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTAtt                   <  8:328723/150‑1 (MQ=255)
                                                                                                                                      ccGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATc               >  5:66961/1‑150 (MQ=255)
                                                                                                                                       cGTTTGTTTGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCaa             <  6:229129/151‑1 (MQ=255)
                                                                                                                                        gtttgtttGCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAAc            >  3:1022977/1‑151 (MQ=255)
                                                                                                                                         tttgtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAAcc           >  1:438028/1‑151 (MQ=255)
                                                                                                                                           tgtttgCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt         >  6:1039240/1‑151 (MQ=255)
                                                                                                                                           tgtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt         >  7:1383223/1‑151 (MQ=255)
                                                                                                                                            gtttgCAGAGTATAGTTAACCATCTTACAAGTTAATCGTGATCGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCGCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATAGTTCCGCCAGCGAGTGGGTATTTATCAAGCGTa        <  4:1075607/151‑1 (MQ=255)
                                                                                                                                            gtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTa        <  7:1140839/151‑1 (MQ=255)
                                                                                                                                            gtttgCAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGACGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGt         >  1:1649264/1‑150 (MQ=255)
                                                                                                                                                 cAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCTGCGGTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt   <  6:688539/151‑1 (MQ=255)
                                                                                                                                                 cAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt   <  1:1452366/151‑1 (MQ=255)
                                                                                                                                                 cAGAGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTACGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGAttt   >  1:1041850/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTAATAGGCGGTATTGCTCACCGGTTTCCATGAACTGCCATTCAGACTGAACTGCATATCGTACGCCCCGACGGCCGAGGCTAACGACGAGTTGGCGATCGCCGGGAATATATGGATTGAGGTGTTGCTGAGTCCGTTTGTTTGCAGGGTATAGTTAACCATCTTACAACTTAATCCTGAACGGCTGCCGATGGTTTGCGTCCGGCAGTCGGCGTTCCCTTCGCCCAGAGTCGGTACTCCGTCGCTGTTGATAAATACTTCCGCCAGCGAGTGGGTATTTATCAACCGTAGATTTG  >  NC_000913/305875‑306171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑