Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,388,120 C→T A417A (GCG→GCA mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,1200CT96.7% 1512.7 / 23.6 454A417A (GCG→GCAmscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base C (5/10);  new base T (217/222);  total (222/232)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 460 total aligned reads displayed.

CACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACC  >  NC_000913/4387972‑4388269
                                                                                                                                                    |                                                                                                                                                     
cacTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAgtgc                                                                                                                                                     <  4:1638350/151‑1 (MQ=255)
   tGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCAACGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtc                                                                                                                                                  >  4:1673866/1‑151 (MQ=255)
    ggCTTTGGGCTACTGACTGAAGGTGTCCAGGGATATGAGAGGAAGGAGATCCTGGGAGATTTCGAGCGGCCAGGCGATGGTCATCGGGCGCACGTCTGAGGTCGTGAACAGATAGCGGTGGGTTGCTTCGTTCACCTCTTTCAGTGCAt                                                                                                                                                   <  3:1412346/149‑1 (MQ=255)
     gCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCAtcct                                                                                                                                                >  7:48429/1‑151 (MQ=255)
         tGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCa                                                                                                                                             <  1:1426494/150‑1 (MQ=255)
          gCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAg                                                                                                                                            <  7:1256071/150‑1 (MQ=255)
          gCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGt                                                                                                                                           >  7:196576/1‑151 (MQ=255)
            ccAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTg                                                                                                                                         <  7:1004251/151‑1 (MQ=255)
            ccAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCACTTg                                                                                                                                         <  4:469894/151‑1 (MQ=255)
             cAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGc                                                                                                                                        >  8:605245/1‑151 (MQ=255)
              aaCTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGcc                                                                                                                                       >  8:254458/1‑151 (MQ=255)
               actgactgAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGccc                                                                                                                                      <  1:72976/151‑1 (MQ=255)
               actgactgAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGccc                                                                                                                                      <  2:54011/151‑1 (MQ=255)
                   actgaAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTg                                                                                                                                  >  6:683213/1‑151 (MQ=255)
                    ctgaAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTgg                                                                                                                                 >  7:418047/1‑151 (MQ=255)
                     tgaAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTgg                                                                                                                                 >  2:143336/1‑150 (MQ=255)
                        aGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGAc                                                                                                                             <  4:586414/151‑1 (MQ=255)
                              ccAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCCCGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTc                                                                                                                         >  5:29969/1‑149 (MQ=255)
                               cAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGc                                                                                                                      >  2:1485457/1‑151 (MQ=255)
                                 gCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCtt                                                                                                                    <  3:157630/151‑1 (MQ=255)
                                  cGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTg                                                                                                                   <  1:427180/151‑1 (MQ=255)
                                    aaaTGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGt                                                                                                                 <  3:418408/151‑1 (MQ=255)
                                    aaaTGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGt                                                                                                                 >  4:130016/1‑151 (MQ=255)
                                     aaTGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTc                                                                                                                >  1:308109/1‑151 (MQ=255)
                                        gagaCGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAg                                                                                                              <  4:830288/150‑1 (MQ=255)
                                          gactacGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGTCGCACGTCAGAGGTCCAGAACACATAGCGGTGCGTTGCTTCGTACACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGtgc                                                                                                           >  4:949956/1‑149 (MQ=255)
                                            cgacgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTcc                                                                                                          >  3:30565/1‑150 (MQ=255)
                                            cgacgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCa                                                                                                         <  6:616258/151‑1 (MQ=255)
                                            cgacgCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCa                                                                                                         <  5:1272323/151‑1 (MQ=255)
                                                  aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAAtagta                                                                                                   <  6:1489564/151‑1 (MQ=255)
                                                  aGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAAtagta                                                                                                   <  6:873725/151‑1 (MQ=255)
                                                         gggCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCgtgt                                                                                             <  6:1130675/150‑1 (MQ=255)
                                                         gggCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTc                                                                                            >  1:1222843/1‑151 (MQ=255)
                                                          ggCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCg                                                                                           >  5:150224/1‑151 (MQ=255)
                                                             gATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGccc                                                                                        >  8:1556092/1‑150 (MQ=255)
                                                             gATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCa                                                                                        <  6:1647762/151‑1 (MQ=255)
                                                              aTTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTCTCGCCAc                                                                                       >  7:593407/1‑151 (MQ=255)
                                                                 tCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCt                                                                                    >  3:114484/1‑151 (MQ=255)
                                                                  ccAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTg                                                                                   <  5:792546/151‑1 (MQ=255)
                                                                  ccAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTg                                                                                   <  1:777051/151‑1 (MQ=255)
                                                                   cAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGc                                                                                  <  6:259201/151‑1 (MQ=255)
                                                                     gCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGca                                                                                 >  8:1262398/1‑150 (MQ=255)
                                                                      cGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGcaac                                                                               >  4:1161325/1‑151 (MQ=255)
                                                                              cGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGtt                                                                       >  5:509217/1‑151 (MQ=255)
                                                                              cGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGtt                                                                       >  5:961827/1‑151 (MQ=255)
                                                                               gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTc                                                                      <  3:1242054/151‑1 (MQ=255)
                                                                               gATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTACATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTc                                                                      <  1:1015426/151‑1 (MQ=255)
                                                                                aTGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTc                                                                      >  4:42418/1‑150 (MQ=255)
                                                                                      aTCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACt                                                               <  8:535535/151‑1 (MQ=255)
                                                                                        cGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTc                                                              >  3:903541/1‑150 (MQ=255)
                                                                                        cGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTc                                                              >  3:45451/1‑150 (MQ=255)
                                                                                        cGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCa                                                             <  7:958450/151‑1 (MQ=255)
                                                                                          ggCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACg                                                           <  1:1089144/151‑1 (MQ=255)
                                                                                           gcggACGTCTGAGGTCCAGAACAGATAGCGGTGCGTTGCTTAGTTCACCTCTTTCAGTGCATCCTGGAGTTGCCAGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCAGCCTTCAAGAATGAGTTCAGCAACTCACGc                                                          <  1:1601135/151‑1 (MQ=255)
                                                                                            cgcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCt                                                         <  8:738145/151‑1 (MQ=255)
                                                                                            cgcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCt                                                         >  7:1421047/1‑151 (MQ=255)
                                                                                             gcACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTg                                                        >  3:988181/1‑151 (MQ=255)
                                                                                              cACGTCAGAGGTCCAGAACAGATAGCGTTGAGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGTGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGa                                                       <  3:550240/151‑1 (MQ=255)
                                                                                              cACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTg                                                        >  6:1372643/1‑150 (MQ=255)
                                                                                                  tCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgg                                                   >  2:465372/1‑150 (MQ=255)
                                                                                                  tCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgc                                                   >  3:671380/1‑151 (MQ=255)
                                                                                                    agagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCTCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcgc                                                 >  5:1562087/1‑151 (MQ=255)
                                                                                                     gagGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcgc                                                 >  7:879231/1‑150 (MQ=255)
                                                                                                      agGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTgcgc                                                 >  2:581959/1‑149 (MQ=255)
                                                                                                      agGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAg                                               <  6:36795/151‑1 (MQ=255)
                                                                                                       ggTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGt                                              >  7:706595/1‑151 (MQ=255)
                                                                                                       ggTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGt                                              >  2:1251414/1‑151 (MQ=255)
                                                                                                       ggTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGt                                              >  5:1670174/1‑151 (MQ=255)
                                                                                                        gTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGtt                                             <  1:942127/151‑1 (MQ=255)
                                                                                                        gTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGtt                                             <  7:1588248/151‑1 (MQ=255)
                                                                                                         tCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGATGAGTGCGCAGttt                                            >  2:689619/1‑150 (MQ=255)
                                                                                                          ccAGAACAGATAGCGGTGCGTTGCTTGGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgt                                           <  7:1142157/151‑1 (MQ=255)
                                                                                                          ccAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTtgt                                           <  3:1507437/151‑1 (MQ=255)
                                                                                                             gAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCt                                        >  1:477117/1‑151 (MQ=255)
                                                                                                              aaCAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCtt                                       <  3:1057533/151‑1 (MQ=255)
                                                                                                                cAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTcc                                     >  5:1094638/1‑151 (MQ=255)
                                                                                                                 aGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTcc                                     >  5:1400714/1‑150 (MQ=255)
                                                                                                                 aGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCa                                    <  2:783180/151‑1 (MQ=255)
                                                                                                                  gATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAg                                   <  7:313813/151‑1 (MQ=255)
                                                                                                                     aGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAAt                                >  2:918710/1‑151 (MQ=255)
                                                                                                                     aGCGGTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAAt                                <  8:344106/151‑1 (MQ=255)
                                                                                                                        ggTGCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACg                             <  2:1056713/151‑1 (MQ=255)
                                                                                                                           gCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGtt                          >  8:1663521/1‑151 (MQ=255)
                                                                                                                           gCGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGt                           <  2:347096/150‑1 (MQ=255)
                                                                                                                            cGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGttt                         >  2:873540/1‑151 (MQ=255)
                                                                                                                            cGTTGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGttt                         <  4:275290/151‑1 (MQ=255)
                                                                                                                             gTTGCTTCGTGCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGtttt                        <  1:54394/151‑1 (MQ=255)
                                                                                                                              ttGCTTCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTg                       >  3:24965/1‑151 (MQ=255)
                                                                                                                                   tCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTc                    <  5:61920/149‑1 (MQ=255)
                                                                                                                                   tCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCgg                  >  8:1217026/1‑151 (MQ=255)
                                                                                                                                   tCGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCg                   <  6:587291/150‑1 (MQ=255)
                                                                                                                                    cGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGc                 >  8:1343317/1‑151 (MQ=255)
                                                                                                                                    cGTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGc                 >  4:1561827/1‑151 (MQ=255)
                                                                                                                                     gTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGTAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGc                 >  1:121162/1‑150 (MQ=255)
                                                                                                                                     gTTCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGc                 >  4:1257885/1‑150 (MQ=255)
                                                                                                                                       tCACCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGt              >  2:245481/1‑151 (MQ=255)
                                                                                                                                         aCCTCTTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCa            <  2:1529512/151‑1 (MQ=255)
                                                                                                                                             ctTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCgg        >  5:387980/1‑151 (MQ=255)
                                                                                                                                             ctTTCAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCgg        >  4:1510580/1‑151 (MQ=255)
                                                                                                                                                 cAGTGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTg     >  7:1286449/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACTGGCTTTGCCCAACTGACTGAAGGTGTCCAGCGAAATGAGACGACGCAGATCCTGGGCGATTTCCAGCGGCCAGGCGATGGTCATCGGGCGCACGTCAGAGGTCCAGAACAGATAGCGGTGCGTTGCTTCGTTCACCTCTTTCAGCGCATCCTCCAGTTGCCCGTTGGAGACTTTCAGCTTGGTCAGTTCCAATAGTAGCGTGTCGCCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACC  >  NC_000913/4387972‑4388269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: