BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,426,929	C→T	C165Y (TGC→TAC)	yjfZ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,426,929	0	C	T	96.7%	1515.1 / 24.3	450	C165Y (TGC→TAC)	yjfZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (6/9); new base T (234/200); total (240/210)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 450 total aligned reads displayed.

AGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTC  >  NC_000913/4426780‑4427078

aGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCa                                                                                                                                                      <  1:920466/151‑1 (MQ=255)

   aaCGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAAtt                                                                                                                                                   <  5:670002/151‑1 (MQ=255)

    aCGCAGACCAATTTTCAAATCATGGGTTAAAACAGAATACGCGTATAGCAATGCAGCAATGATGAATCAGAGGGAATTAGAAACGACTGAAACAGTCCGAACATGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAAtt                                                                                                                                                   >  1:773449/1‑150 (MQ=255)

     cgcagagataTTTTCAAATCATTGTGTAAATCAGAATACGCGTTTAGCAATGTAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCTAAGTTTGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAg                                                                                                                                                 <  2:595180/142‑1 (MQ=255)

      gCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAgg                                                                                                                                                <  3:350309/151‑1 (MQ=255)

         gACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCAt                                                                                                                                             <  7:1126753/151‑1 (MQ=255)

          aCCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCAtt                                                                                                                                            >  1:439575/1‑151 (MQ=255)

            cAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTg                                                                                                                                          >  8:1456059/1‑151 (MQ=255)

            cAATTTTAAAATAATGGGGTAAATCAGAATACGAGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAAAGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTTTTGGTAGTTCAGATGACCAGTAATTAgcaatatt                                                                                                                                          >  4:326793/1‑144 (MQ=255)

               ttttCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGc                                                                                                                                       >  6:635373/1‑151 (MQ=255)

                 ttCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGc                                                                                                                                       >  5:1073709/1‑149 (MQ=255)

                   cAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAgg                                                                                                                                    <  3:1058008/150‑1 (MQ=255)

                    aaaTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGaa                                                                                                                                  <  7:1398803/151‑1 (MQ=255)

                    aaaTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGa                                                                                                                                   >  5:1601845/1‑150 (MQ=255)

                     aaTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAg                                                                                                                                 <  3:1125978/151‑1 (MQ=255)

                     aaTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAg                                                                                                                                 <  6:1611346/151‑1 (MQ=255)

                      aTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAgg                                                                                                                                <  6:308879/151‑1 (MQ=255)

                      aTCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAgg                                                                                                                                <  4:629709/151‑1 (MQ=255)

                        cATGGGGTAAATCAGAATACGCGTTTAGGAATGAAGCAATGATGAATCGGAGGGCTTTAGATACGCCTGTAACAGTGCGAAGTTTGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGt                                                                                                                              <  8:1270691/151‑1 (MQ=255)

                        cATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGt                                                                                                                              >  6:1182116/1‑151 (MQ=255)

                         aTGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGtt                                                                                                                             <  7:677953/151‑1 (MQ=255)

                            gggTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTAc                                                                                                                           >  3:248653/1‑150 (MQ=255)

                               tAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGAAGGAAGGGTTACAACg                                                                                                                       >  6:1444059/1‑151 (MQ=255)

                                aaaTCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACg                                                                                                                       <  5:1357937/150‑1 (MQ=255)

                                  aTCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTAt                                                                                                                    >  3:745795/1‑151 (MQ=255)

                                   tCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATc                                                                                                                   >  7:1467680/1‑151 (MQ=255)

                                        aTACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATc                                                                                                              >  7:388955/1‑151 (MQ=255)

                                           cgcgTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGtt                                                                                                           <  4:1021999/151‑1 (MQ=255)

                                              gTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTgg                                                                                                        >  6:206331/1‑151 (MQ=255)

                                                   gCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGt                                                                                                   <  8:814048/151‑1 (MQ=255)

                                                    cAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGt                                                                                                   >  8:40591/1‑150 (MQ=255)

                                                     aaTGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGtat                                                                                                 >  7:93476/1‑151 (MQ=255)

                                                     aaTGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGtat                                                                                                 <  8:728894/151‑1 (MQ=255)

                                                      aTGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGTCCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGtata                                                                                                <  2:1393824/151‑1 (MQ=255)

                                                        gcagcaATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGt                                                                                              <  8:180096/151‑1 (MQ=255)

                                                           gcaATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTg                                                                                           >  1:384711/1‑151 (MQ=255)

                                                            caATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCAgg                                                                                          >  4:1454626/1‑151 (MQ=255)

                                                             aaTGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTgg                                                                                          >  2:648844/1‑150 (MQ=255)

                                                              atgatgAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAg                                                                                        <  2:1499928/151‑1 (MQ=255)

                                                                gatgaATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGt                                                                                       <  3:1475852/150‑1 (MQ=255)

                                                                 atgaATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTAc                                                                                     >  3:86949/1‑151 (MQ=255)

                                                                    aaTCCTAGGGGATTAGAGACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGGTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCAt                                                                                  <  2:914099/151‑1 (MQ=255)

                                                                    aaTCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCAt                                                                                  <  6:1088939/151‑1 (MQ=255)

                                                                    aaTCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCAt                                                                                  >  6:409097/1‑151 (MQ=255)

                                                                    aaTCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCAt                                                                                  <  4:483331/151‑1 (MQ=255)

                                                                    aaTCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCAt                                                                                  >  2:698956/1‑151 (MQ=255)

                                                                       ccGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAc                                                                               <  3:1251752/151‑1 (MQ=255)

                                                                        cGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAcc                                                                              >  4:1609851/1‑151 (MQ=255)

                                                                         gAGGGCATTAGAAACGCCTGTAACAGTGCGGAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAcc                                                                              >  1:1277005/1‑150 (MQ=255)

                                                                         gAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAcc                                                                              >  4:1364025/1‑150 (MQ=255)

                                                                         gAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCACGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAcc                                                                              >  1:1481251/1‑150 (MQ=255)

                                                                           gggCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAgg                                                                           <  7:1556520/151‑1 (MQ=255)

                                                                               aTTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTccc                                                                       >  5:384074/1‑151 (MQ=255)

                                                                                   gAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCaaa                                                                    >  1:704415/1‑150 (MQ=255)

                                                                                    aaaCGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAAc                                                                  >  6:1516928/1‑151 (MQ=255)

                                                                                     aaCGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACATCGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAAcc                                                                 <  2:169654/151‑1 (MQ=255)

                                                                                          cTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTc                                                            >  7:573852/1‑151 (MQ=255)

                                                                                           tGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCg                                                           >  8:1106940/1‑151 (MQ=255)

                                                                                            gTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCgt                                                          >  3:797031/1‑151 (MQ=255)

                                                                                              aaCAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTAACATAACCAGGTCCCAAAACCACTTCgtgt                                                        >  2:425916/1‑151 (MQ=255)

                                                                                               aCAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCgtgta                                                       <  6:534745/151‑1 (MQ=255)

                                                                                                     cGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCgtgtattgtgt                                                 <  1:164572/151‑1 (MQ=255)

                                                                                                      gAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCCCTTCgtgtattgtgtt                                                >  4:1097614/1‑150 (MQ=255)

                                                                                                      gAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCgtgtattgtgt                                                 >  5:269098/1‑150 (MQ=255)

                                                                                                      gAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATCACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCAATCGTTTGGCCGGTATAGTCTGGACTACCATAACCAGGTCCCAAAACCACTTCctgtaatgaga                                                 >  1:1028514/1‑147 (MQ=255)

                                                                                                         gTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTAttt                                             <  3:909641/151‑1 (MQ=255)

                                                                                                             gTCTATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAAGCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTg                                         <  5:972635/151‑1 (MQ=255)

                                                                                                               cGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGtt                                       >  2:119148/1‑151 (MQ=255)

                                                                                                               cGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGtt                                       >  3:5193/1‑151 (MQ=255)

                                                                                                               cGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGtt                                       >  2:1284459/1‑151 (MQ=255)

                                                                                                                gATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTg                                      <  4:78766/151‑1 (MQ=255)

                                                                                                                gATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTg                                      >  5:758593/1‑151 (MQ=255)

                                                                                                                gATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTg                                      >  1:239238/1‑151 (MQ=255)

                                                                                                                 atatAAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTgg                                     <  5:1414727/151‑1 (MQ=255)

                                                                                                                  tataAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTggg                                    >  7:124203/1‑151 (MQ=255)

                                                                                                                   ataAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGt                                   <  5:783726/151‑1 (MQ=255)

                                                                                                                   ataAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGt                                   <  8:335074/151‑1 (MQ=255)

                                                                                                                    taAAGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGtt                                  >  6:741793/1‑151 (MQ=255)

                                                                                                                     aaaGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTc                                 <  6:93166/151‑1 (MQ=255)

                                                                                                                      aaGATCGATTGTTGGTAGTTCTGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                >  3:1352066/1‑151 (MQ=255)

                                                                                                                      aaGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                >  4:701216/1‑151 (MQ=255)

                                                                                                                      aaGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                <  2:989742/151‑1 (MQ=255)

                                                                                                                       aGATCGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCg                                >  2:283869/1‑150 (MQ=255)

                                                                                                                           cGATTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGa                           <  4:1550374/151‑1 (MQ=255)

                                                                                                                             aTTGTTGGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTACCAAAACCACTTCGTGTATTGTGTATTTACTGTTTGGTTCGAAGGAtt                         >  7:827263/1‑151 (MQ=255)

                                                                                                                               tgttgGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGTAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTc                        <  2:1077240/150‑1 (MQ=255)

                                                                                                                               tgttgGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCg                       >  5:436124/1‑151 (MQ=255)

                                                                                                                                gttgGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCgc                      >  4:431400/1‑150 (MQ=255)

                                                                                                                                gttgGTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCg                       <  3:1561740/150‑1 (MQ=255)

                                                                                                                                  tgGAAGTTCAGATGACCAGAAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTGCGAAGGATTCGAc                     <  6:1321377/150‑1 (MQ=255)

                                                                                                                                    gTAGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAACGATTCGACAt                   >  1:631892/1‑150 (MQ=255)

                                                                                                                                      aGTTCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTGCAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCt                 <  1:1367656/150‑1 (MQ=255)

                                                                                                                                        ttCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACTACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTg               <  2:1006466/150‑1 (MQ=255)

                                                                                                                                         tCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGc              <  4:886410/150‑1 (MQ=255)

                                                                                                                                         tCAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGc              >  1:1164326/1‑150 (MQ=255)

                                                                                                                                          cAGATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCaa            <  5:636334/151‑1 (MQ=255)

                                                                                                                                            gATGACCAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCaata          <  8:1604585/151‑1 (MQ=255)

                                                                                                                                                 ccAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAatat     <  8:474519/151‑1 (MQ=255)

                                                                                                                                                  cAGTAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATAtt    <  2:1550511/151‑1 (MQ=255)

                                                                                                                                                    gCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTc  >  3:615431/1‑151 (MQ=255)

AGGAACGCAGACCAATTTTCAAATCATGGGGTAAATCAGAATACGCGTTTAGCAATGCAGCAATGATGAATCCGAGGGCATTAGAAACGCCTGTAACAGTGCGAAGTTGGTCGATATAAAGATCGATTGTTGGTAGTTCAGATGACCAGCAATTAGGCATTTGGGCAGGAAGGGTTACAACGTATCCTATCGTTTGGCCGGTATAGTCTGGAGTACCATAACCAGGTCCCAAAACCACTTCGTGTATTGTGTATTTACTGTTGGGTTCGAAGGATTCGACATCTTGCAATAAATATTTC  >  NC_000913/4426780‑4427078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`