Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,435,124 C→T W481* (TGG→TAG)  cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,435,1240CT96.8% 1364.2 / 19.7 405W481* (TGG→TAG) cpdB2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (4/9);  new base T (186/206);  total (190/215)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 406 total aligned reads displayed.

GGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATT  >  NC_000913/4434977‑4435269
                                                                                                                                                   |                                                                                                                                                 
ggCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGt                                                                                                                                                >  5:59766/1‑151 (MQ=255)
ggCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGt                                                                                                                                                >  6:1089001/1‑151 (MQ=255)
     gCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTtgat                                                                                                                                            <  3:1564622/150‑1 (MQ=255)
      ccATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTtgatga                                                                                                                                          <  8:671862/151‑1 (MQ=255)
       cATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAg                                                                                                                                         <  6:443818/151‑1 (MQ=255)
        aTTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAGCCATCCTAGTTGATGAGt                                                                                                                                        >  1:996190/1‑151 (MQ=255)
        aTTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTCATGAGt                                                                                                                                        >  1:1507216/1‑151 (MQ=255)
         ttAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTg                                                                                                                                       >  4:324962/1‑151 (MQ=255)
            aaGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACt                                                                                                                                    <  8:1413584/151‑1 (MQ=255)
            aaGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACt                                                                                                                                    >  8:787958/1‑151 (MQ=255)
            aaGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACt                                                                                                                                    >  5:1357053/1‑151 (MQ=255)
              ggTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACtgt                                                                                                                                  >  4:1204793/1‑151 (MQ=255)
                 gaGGTTCTTAATCCTTTCCGCATTGGCATTAATGATCTGTCACTCGCCTTCATAACGGTCGGGCTGGGTAACATGAAGCTGATAATTAAGTCCATCAATAACTTCAAAGTTATAAGTTCGTAAACCATCCTAGTTGATGAGTGACtgtgtt                                                                                                                               <  8:30948/150‑3 (MQ=255)
                 cAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAATTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGt                                                                                                                               >  8:1547763/1‑151 (MQ=255)
                  aGGTTCTTAATGATTTGCTCATTTTCATTAATCATCTGGCACTCGCCGTCTTAACGGGCGGGGTTGTTAACATCAATCTGATAATTCACACCATAAATAACATCAAATTTATAAGTGCGTAAACCATCCTAGTTGATGAGTGACtgttttt                                                                                                                              <  8:423331/151‑5 (MQ=255)
                    gTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTc                                                                                                                            <  5:73617/151‑1 (MQ=255)
                      tCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGt                                                                                                                          >  3:179597/1‑151 (MQ=255)
                         tAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGt                                                                                                                          <  2:1561338/148‑1 (MQ=255)
                          aaTCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCt                                                                                                                       <  5:41580/150‑1 (MQ=255)
                           aTCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGt                                                                                                                     >  1:768293/1‑151 (MQ=255)
                             ccTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACGATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGtt                                                                                                                    <  7:25164/150‑1 (MQ=255)
                               tttCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTggg                                                                                                                 >  5:661476/1‑151 (MQ=255)
                               tttCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTggg                                                                                                                 >  2:594734/1‑151 (MQ=255)
                               tttCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTggg                                                                                                                 >  2:141085/1‑151 (MQ=255)
                                 tCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGAt                                                                                                               >  2:1021257/1‑151 (MQ=255)
                                    gCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCa                                                                                                             >  2:201161/1‑150 (MQ=255)
                                     cATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAAt                                                                                                           >  3:1151413/1‑151 (MQ=255)
                                      aTTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCCGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATc                                                                                                          >  7:624545/1‑151 (MQ=255)
                                         ggCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTgg                                                                                                       >  6:201455/1‑151 (MQ=255)
                                          gCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGt                                                                                                      <  2:480186/151‑1 (MQ=255)
                                          gCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGt                                                                                                      >  6:1010954/1‑151 (MQ=255)
                                           cATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGtt                                                                                                     <  1:1438534/151‑1 (MQ=255)
                                            aTTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACCCCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGAGCAATCTGGtt                                                                                                     >  1:102007/1‑150 (MQ=255)
                                                atgatcTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACt                                                                                                <  6:345928/148‑1 (MQ=255)
                                                atcatcTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACt                                                                                                <  5:776990/151‑1 (MQ=255)
                                                    tcTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTcc                                                                                            >  7:681622/1‑151 (MQ=255)
                                                       ggCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTcc                                                                                            <  3:1020202/148‑1 (MQ=255)
                                                       ggCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCcgc                                                                                         <  1:630062/151‑1 (MQ=255)
                                                         cACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCgg                                                                                       <  3:958668/151‑1 (MQ=255)
                                                         cACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCgg                                                                                       >  5:194084/1‑151 (MQ=255)
                                                          ccTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGa                                                                                      <  4:65344/150‑1 (MQ=255)
                                                          aCTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCgg                                                                                       >  5:1241761/1‑150 (MQ=255)
                                                          aCTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGa                                                                                      <  8:1240977/151‑1 (MQ=255)
                                                          aCTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGa                                                                                      <  4:1453389/151‑1 (MQ=255)
                                                          aCTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGa                                                                                      <  4:952940/151‑1 (MQ=255)
                                                            tCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGc                                                                                    >  3:797882/1‑151 (MQ=255)
                                                             cGCCGTCATAACGGGCGGGCTGGGTAAGATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGGGGAg                                                                                     <  1:1364963/149‑1 (MQ=255)
                                                               ccGTCATAACGGGCGGGCTGGGTAACATCACTCTGCTAATTCCCCCCATCAATAACATCAAAGTTATAAGTGCGGCAACCCTCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCACTCTGGTTAAACTGGCCCGCGgcgctcg                                                                                 >  6:762639/1‑148 (MQ=255)
                                                                   cATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATATGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGGTGTTGGGATCAATCTGGTTATACTGTCCCGCGGAGCACTCCa                                                                              <  6:934564/150‑1 (MQ=255)
                                                                   cATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCa                                                                              <  5:88901/150‑1 (MQ=255)
                                                                   cATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCa                                                                              <  1:811911/150‑1 (MQ=255)
                                                                    aTAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGATAAACTGTCCCGCGGAGCACTCCAGc                                                                            >  2:1219305/1‑151 (MQ=255)
                                                                        cgggcgggCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCAct                                                                        <  1:1266133/151‑1 (MQ=255)
                                                                            cgggcTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCttt                                                                    >  4:1089653/1‑151 (MQ=255)
                                                                               gcTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCAc                                                                 >  3:1467805/1‑151 (MQ=255)
                                                                                  gggTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGATGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACctc                                                              >  6:609528/1‑151 (MQ=255)
                                                                                   ggTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACctct                                                             >  1:37203/1‑151 (MQ=255)
                                                                                    ggtaaaagaagggtaTAATTCACAACAACAATAACCTGAAAGTTAATAGGGCGGAAACCATCCTAGTTGATGAGTGACTGTGGGTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCtt                                                            <  8:1285846/137‑1 (MQ=255)
                                                                                    gTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCtt                                                            <  1:1605467/151‑1 (MQ=255)
                                                                                     tAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCttt                                                           >  5:978329/1‑151 (MQ=255)
                                                                                      aaCATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCttt                                                           >  3:673856/1‑150 (MQ=255)
                                                                                      aaCATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTa                                                          <  5:1193013/151‑1 (MQ=255)
                                                                                           cAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCt                                                     <  3:549840/151‑1 (MQ=255)
                                                                                                 gATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCttt                                               >  7:1214487/1‑151 (MQ=255)
                                                                                                     aTTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTcacc                                           >  3:996571/1‑151 (MQ=255)
                                                                                                       tCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTcaccac                                         >  2:844160/1‑151 (MQ=255)
                                                                                                       tCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTcaccac                                         >  6:689579/1‑151 (MQ=255)
                                                                                                          cacCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAAt                                      <  8:1004257/151‑1 (MQ=255)
                                                                                                            ccATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCa                                    <  4:518127/151‑1 (MQ=255)
                                                                                                             cATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATc                                     >  5:547085/1‑149 (MQ=255)
                                                                                                               tCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCCCCTCTTTACCGCTGGCTTTCACCACAATCAGCg                                 >  8:86577/1‑151 (MQ=255)
                                                                                                               tCAATAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACACTCAGCg                                 >  8:536641/1‑151 (MQ=255)
                                                                                                                  aTAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTAt                              >  3:449310/1‑151 (MQ=255)
                                                                                                                  aTAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTAt                              >  7:1088018/1‑151 (MQ=255)
                                                                                                                   tAACATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTAtt                             >  3:397144/1‑151 (MQ=255)
                                                                                                                    aaCATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTg                            <  6:1596456/151‑1 (MQ=255)
                                                                                                                    aaCATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTg                            <  8:1636529/151‑1 (MQ=255)
                                                                                                                      cATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTggg                          <  1:531010/151‑1 (MQ=255)
                                                                                                                      cATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTggg                          <  2:1043826/151‑1 (MQ=255)
                                                                                                                      cATCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTggg                          <  7:1608318/151‑1 (MQ=255)
                                                                                                                       aTCAAAGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTggg                          >  5:297725/1‑150 (MQ=255)
                                                                                                                            aGTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATag                      >  5:1585737/1‑149 (MQ=255)
                                                                                                                             gTTATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAgaga                   <  3:1232848/151‑1 (MQ=255)
                                                                                                                              ttATAAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATag                      >  1:28727/1‑147 (MQ=255)
                                                                                                                               tataAGTGCGGAAACCATCCTAGTTGATGAGTGCCTGTGGGTTCTTGATGTTGGGATCAATCTGGTTAAACTGTACCGAGGAGCACTCCATACACTCTTTCACCTCTTTACCGCTGGATTTCACCACCATCAGCGTATTGTGATAGAGAt                  <  4:153659/150‑1 (MQ=255)
                                                                                                                               tataAGTGCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGAt                  <  7:1355112/150‑1 (MQ=255)
                                                                                                                                  aaGTGCGGAAACCATCCTAGTTGATGAGTGAGTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACAGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAg              <  4:239646/151‑1 (MQ=255)
                                                                                                                                     tGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATaa                >  2:347633/1‑146 (MQ=255)
                                                                                                                                      gCGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATc           <  8:1100989/150‑1 (MQ=255)
                                                                                                                                       cGGAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCACTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATaa                >  6:1423761/1‑144 (MQ=255)
                                                                                                                                        ggAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggc        >  7:405007/1‑151 (MQ=255)
                                                                                                                                         gAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggcg       >  5:1477324/1‑151 (MQ=255)
                                                                                                                                         gAAACCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggcg       >  7:938680/1‑151 (MQ=255)
                                                                                                                                         gAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggcg       <  2:654286/151‑1 (MQ=255)
                                                                                                                                          aaaCCATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggcgg      <  2:913372/151‑1 (MQ=255)
                                                                                                                                          aaaCCATCCTAGGTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATcggcnn      <  6:1521346/151‑3 (MQ=255)
                                                                                                                                             ccATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACGACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCAt   <  7:1496693/151‑1 (MQ=255)
                                                                                                                                              cATCCTAGTTGATGAGTGACTGTGGTTTCGTGGTGTTGGGATCAATCTGGTTAAACTGTCCCGCTGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACGACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCAtt  <  2:1264141/151‑1 (MQ=255)
                                                                                                                                              cATCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCAtt  <  3:134196/151‑1 (MQ=255)
                                                                                                                                               aTCCTAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTGACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCAtt  >  4:1315077/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCTTGCCATTAAAGGTCAGGTTCTTAATCCTTTCCGCATTGGCATTAATCATCTGGCACTCGCCGTCATAACGGGCGGGCTGGGTAACATCAATCTGATAATTCACACCATCAATAACATCAAAGTTATAAGTGCGGAAACCATCCCAGTTGATGAGTGACTGTGGTTTCGTGCTGTTGGGATCAATCTGGTTAAACTGTCCCGCGGAGCACTCCAGCCACTCTTTCACCTCTTTACCGCTGGCTTTCACCACAATCAGCGTATTGGGATAGAGATAAAGATCGGCGGCATT  >  NC_000913/4434977‑4435269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: