Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,435,750 C→T G272G (GGG→GGA cpdB ← 2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,435,7500CT97.4% 1320.8 / 12.5 390G272G (GGG→GGAcpdB2':3'‑cyclic‑nucleotide 2'‑phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base T (182/197);  total (190/200)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 392 total aligned reads displayed.

AAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCC  >  NC_000913/4435602‑4435897
                                                                                                                                                    |                                                                                                                                                   
aaaTCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGcccc                                                                                                                                                   <  6:245144/151‑1 (MQ=255)
 aaTCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCt                                                                                                                                                  >  8:729971/1‑151 (MQ=255)
      gTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACGGCTGTGATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGATCACCATTCAGCGTGCCTTTGGCGATATCAGCTCCtt                                                                                                                                                 <  8:1300932/147‑1 (MQ=255)
       tCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGat                                                                                                                                             >  7:1364433/1‑150 (MQ=255)
         gTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATc                                                                                                                                          <  2:1118700/151‑1 (MQ=255)
           gCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCa                                                                                                                                         <  6:693895/150‑1 (MQ=255)
             ttCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGc                                                                                                                                       >  3:1336474/1‑150 (MQ=255)
              tCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACCTGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCa                                                                                                                                      >  8:1171652/1‑150 (MQ=255)
              tCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCa                                                                                                                                      <  4:568242/150‑1 (MQ=255)
               ccGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaa                                                                                                                                     >  1:293479/1‑150 (MQ=255)
               ccGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTGGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaaa                                                                                                                                    <  4:190048/151‑1 (MQ=255)
                cGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCaaaa                                                                                                                                   <  7:177893/151‑1 (MQ=255)
                 gCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAAt                                                                                                                                  >  2:736775/1‑151 (MQ=255)
                    ttCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATc                                                                                                                                 <  6:116274/149‑1 (MQ=255)
                    ttCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCtt                                                                                                                               >  7:579983/1‑151 (MQ=255)
                     tCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCttt                                                                                                                              >  7:1004332/1‑151 (MQ=255)
                      cGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGCTATCAGCAAAATCttt                                                                                                                              <  4:304385/150‑1 (MQ=255)
                      cGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCttt                                                                                                                              >  5:692865/1‑150 (MQ=255)
                      cGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCttt                                                                                                                              <  7:762672/150‑1 (MQ=255)
                       gCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTAc                                                                                                                            <  2:176656/151‑1 (MQ=255)
                         cTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCt                                                                                                                          >  6:32089/1‑151 (MQ=255)
                           gCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTgg                                                                                                                        <  8:291214/151‑1 (MQ=255)
                               cACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGaa                                                                                                                     <  2:1262111/150‑1 (MQ=255)
                               cACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGaa                                                                                                                     >  7:1288562/1‑150 (MQ=255)
                                aCCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACCCCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGaaaa                                                                                                                   <  8:1105700/151‑1 (MQ=255)
                                 ccTGCCAATTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAAc                                                                                                                  <  5:1051061/151‑1 (MQ=255)
                                    gCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACgg                                                                                                                <  4:425282/150‑1 (MQ=255)
                                    gCCATTTACCGCTGTCATTACTGAGTTGTAAGGCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGc                                                                                                               <  4:962179/151‑1 (MQ=255)
                                       aTTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTg                                                                                                            <  5:1369763/151‑1 (MQ=255)
                                       aTTTACCGCTGTCATTACTGAGTTGTAAGGCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTg                                                                                                            <  8:1075868/151‑1 (MQ=255)
                                           aCCGCTGTCATTACTGAGTTGTAAGTCGACCCCACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCa                                                                                                        <  4:855104/151‑1 (MQ=255)
                                           aCCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGc                                                                                                         <  5:322908/150‑1 (MQ=255)
                                           aCCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGc                                                                                                         <  7:324896/150‑1 (MQ=255)
                                                 gTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGc                                                                                                    <  5:1301173/149‑1 (MQ=255)
                                                  tCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCAACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCa                                                                                                  >  1:1674383/1‑150 (MQ=255)
                                                   cATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCaaa                                                                                                <  3:503998/151‑1 (MQ=255)
                                                   cATTACTGACTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCaa                                                                                                 <  2:903831/150‑1 (MQ=255)
                                                     ttACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAAc                                                                                               >  2:522240/1‑150 (MQ=255)
                                                      tACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACAt                                                                                             >  5:1604401/1‑151 (MQ=255)
                                                        cTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATa                                                                                            >  2:1032117/1‑150 (MQ=255)
                                                         tGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAAt                                                                                          >  2:646277/1‑151 (MQ=255)
                                                         tGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAAt                                                                                          >  4:1247575/1‑151 (MQ=255)
                                                              tGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCg                                                                                      <  1:1375359/150‑1 (MQ=255)
                                                                tAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGtt                                                                                    >  1:1301187/1‑150 (MQ=255)
                                                                tAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGtt                                                                                    >  6:621785/1‑150 (MQ=255)
                                                                tAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGtt                                                                                    >  2:375253/1‑150 (MQ=255)
                                                                 aaGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCTATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTa                                                                                   <  8:527507/150‑1 (MQ=255)
                                                                 aaGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCCTTa                                                                                   >  2:581599/1‑150 (MQ=255)
                                                                 aaGTCGACCACACCAAGATGAGCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTaa                                                                                  <  4:15876/151‑1 (MQ=255)
                                                                  aGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAAc                                                                                 <  8:586567/151‑1 (MQ=255)
                                                                      gACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGccc                                                                             <  2:1173436/151‑1 (MQ=255)
                                                                      gACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGccc                                                                             <  3:1178970/151‑1 (MQ=255)
                                                                         cacaCCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCgg                                                                           >  6:432311/1‑150 (MQ=255)
                                                                            acCAAGATGATCGCCCCACATGCCTCGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAAtt                                                                       <  6:1050875/151‑1 (MQ=255)
                                                                             ccAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAAttt                                                                      <  4:1020258/151‑1 (MQ=255)
                                                                             ccAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAAttt                                                                      <  3:1544660/151‑1 (MQ=255)
                                                                             ccAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGGGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAAttt                                                                      <  2:997391/151‑1 (MQ=255)
                                                                              cAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTc                                                                     <  2:307251/151‑1 (MQ=255)
                                                                                 gatgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACt                                                                  <  7:527773/151‑1 (MQ=255)
                                                                                  atgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTg                                                                 >  8:1611293/1‑151 (MQ=255)
                                                                                  atgatCGCCCCACAGGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTg                                                                 <  6:902461/151‑1 (MQ=255)
                                                                                   tgatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTg                                                                 >  1:742952/1‑150 (MQ=255)
                                                                                    gatCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGa                                                                <  4:115680/150‑1 (MQ=255)
                                                                                     atCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAgg                                                              <  1:694411/151‑1 (MQ=255)
                                                                                      tCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGt                                                             >  3:309607/1‑151 (MQ=255)
                                                                                      tCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGt                                                             >  8:1142053/1‑151 (MQ=255)
                                                                                          cccACATGCCTGGCATTACCGCCGGATCACCATTCAGCGTGCGTTTGGCGATATGAGCTCCTTCGATATCATCAAAATCTTTACCTGGGAATACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGtaatt                                                         <  8:392463/151‑2 (MQ=255)
                                                                                          cccACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGtaata                                                         <  7:1110928/151‑1 (MQ=255)
                                                                                              caTGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACt                                                     >  1:1546035/1‑151 (MQ=255)
                                                                                              caTGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACt                                                     >  7:897868/1‑151 (MQ=255)
                                                                                                 gCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAg                                                  <  3:1078031/151‑1 (MQ=255)
                                                                                                    tGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGttt                                               >  6:761021/1‑151 (MQ=255)
                                                                                                     ggCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGttt                                               <  1:429046/150‑1 (MQ=255)
                                                                                                      gCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTc                                             <  5:1475252/151‑1 (MQ=255)
                                                                                                       cATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTcc                                            >  5:614120/1‑151 (MQ=255)
                                                                                                       cATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTcc                                            <  1:501069/151‑1 (MQ=255)
                                                                                                          tACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGcc                                         >  3:1663341/1‑151 (MQ=255)
                                                                                                          tACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGc                                          <  4:1111437/150‑1 (MQ=255)
                                                                                                           aCCGCCCGAACACCATTCAGCGGGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGcc                                         <  4:719926/150‑1 (MQ=255)
                                                                                                             cgccgGAACACCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCAt                                       >  8:1501335/1‑150 (MQ=255)
                                                                                                                  gAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACttt                                 >  7:468732/1‑151 (MQ=255)
                                                                                                                   aaCACCATTCAGCGTGCCTTAGGCGATATCAGCTCCATCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTta                                <  1:1201137/151‑1 (MQ=255)
                                                                                                                    acacCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTtat                               <  8:225289/151‑1 (MQ=255)
                                                                                                                     cacCATTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTtata                              <  6:1011112/151‑1 (MQ=255)
                                                                                                                         aTTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACgg                           >  5:798702/1‑150 (MQ=255)
                                                                                                                         aTTCAGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGa                          <  3:1413003/151‑1 (MQ=255)
                                                                                                                             aGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCgg                      <  2:138438/151‑1 (MQ=255)
                                                                                                                             aGCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCgg                      <  6:132924/151‑1 (MQ=255)
                                                                                                                              gCGTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGc                     >  5:1170099/1‑151 (MQ=255)
                                                                                                                                gTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAg                   <  6:492799/151‑1 (MQ=255)
                                                                                                                                gTGCCTTTGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGACATCACTTTATACGGATCGGCa                    >  4:1477604/1‑150 (MQ=255)
                                                                                                                                     tttGGCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGc              >  3:836525/1‑151 (MQ=255)
                                                                                                                                        ggCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGccc            <  7:966561/150‑1 (MQ=255)
                                                                                                                                         gCGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCgg          <  6:1437279/151‑1 (MQ=255)
                                                                                                                                          cGATATCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCgg          >  1:1016816/1‑150 (MQ=255)
                                                                                                                                          cGATAGCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCgg          >  3:1167237/1‑150 (MQ=255)
                                                                                                                                             tatCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAAtg      >  5:529551/1‑151 (MQ=255)
                                                                                                                                              atCAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAAtgt     >  3:992895/1‑151 (MQ=255)
                                                                                                                                                cAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGc   <  5:178288/151‑1 (MQ=255)
                                                                                                                                                cAGCTCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGc   >  4:1323461/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAATCGGTCGTGCTTCCGCTTTCGCCTGCGTCACCTGCCATTTACCGCTGTCATTACTGAGTTGTAAGTCGACCACACCAAGATGATCGCCCCACATGCCTGGCATTACCGCCGGAACACCATTCAGCGTGCCTTTGGCGATATCAGCCCCTTCGATATCAGCAAAATCTTTACCTGGGAAAACGGCGTGAGCATGGCCAAACATAATGGCGTTAACGCCCGGAATTTCACTGAGGTAATAAACTGAGTTTTCCGCCATCACTTTATACGGATCGGCAGATAGCCCGGAATGTGCC  >  NC_000913/4435602‑4435897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: