BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,901	C→T	V313M (GTG→ATG)	valS ←	valyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,901	0	C	T	97.1%	1273.2 / 15.8	376	V313M (GTG→ATG)	valS	valyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (5/6); new base T (206/158); total (212/164)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 379 total aligned reads displayed.

AGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTC  >  NC_000913/4482753‑4483048

  gTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTgg                                                                                                                                                 <  3:631832/151‑1 (MQ=255)

   tCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTggg                                                                                                                                                >  4:439824/1‑151 (MQ=255)

   tCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTggg                                                                                                                                                >  1:996967/1‑151 (MQ=255)

   gcGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTggg                                                                                                                                                <  5:508767/150‑1 (MQ=255)

       gCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGgcgc                                                                                                                                             <  3:1398214/150‑1 (MQ=255)

          gTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTc                                                                                                                                         <  5:1472211/151‑1 (MQ=255)

           tttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTc                                                                                                                                         >  8:741505/1‑150 (MQ=255)

            ttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCa                                                                                                                                        <  1:1324602/150‑1 (MQ=255)

            ttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCa                                                                                                                                        <  1:662507/150‑1 (MQ=255)

            ttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCAc                                                                                                                                       >  3:938948/1‑151 (MQ=255)

            ttAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCAc                                                                                                                                       >  1:1459345/1‑151 (MQ=255)

              aaTTTCTTCCAGCAGGCCAAGCTCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACgg                                                                                                                                     <  1:231907/151‑1 (MQ=255)

              aaTTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACgg                                                                                                                                     <  6:291558/151‑1 (MQ=255)

                 ttcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGat                                                                                                                                   >  6:205339/1‑150 (MQ=255)

                 ttcttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGat                                                                                                                                   <  6:900173/150‑1 (MQ=255)

                  tcttcCAGGATGCCAATCGCGTCATCTGCGTCAACGACTTCTTTACGTGCAGCTAAAGGCTGCAGTTTCTGGTTCTCCGGAGGGATTTGGCTTGAATAAACGTCAGATTCGTTACCTTTTGTATCGAACATCTTGGCGCTTTCACGGata                                                                                                                                  <  4:1524837/150‑1 (MQ=255)

                     tcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCG‑TACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGcc                                                                                                                             >  6:396175/1‑151 (MQ=255)

                      ccAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGcc                                                                                                                             <  4:841539/151‑1 (MQ=255)

                      ccAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGAGTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGcc                                                                                                                             >  5:190109/1‑151 (MQ=255)

                        agcagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGt                                                                                                                           >  4:1179075/1‑151 (MQ=255)

                         gcagGCCAAGCGCGTCAACTGCGGCAACGAGTGCTTTACGGGCAGCAAAACGCTCCAGTTTCTGCAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTGCACGGATATCGCCGTc                                                                                                                          <  7:710047/151‑1 (MQ=255)

                          cagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTc                                                                                                                          >  4:656806/1‑150 (MQ=255)

                          cagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTc                                                                                                                          >  3:697600/1‑150 (MQ=255)

                          cagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCa                                                                                                                         <  6:1118252/151‑1 (MQ=255)

                             gCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAg                                                                                                                      <  7:965737/151‑1 (MQ=255)

                               cAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGt                                                                                                                    >  2:520352/1‑151 (MQ=255)

                                aaGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTc                                                                                                                   >  8:1604743/1‑151 (MQ=255)

                                 aGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCa                                                                                                                  <  6:788538/151‑1 (MQ=255)

                                      gTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATg                                                                                                             >  5:1291722/1‑151 (MQ=255)

                                       tCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGAGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGt                                                                                                            >  4:1391842/1‑151 (MQ=255)

                                        cAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGtt                                                                                                           >  1:418461/1‑151 (MQ=255)

                                          aCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                          >  8:300720/1‑150 (MQ=255)

                                           cTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGat                                                                                                        >  7:472777/1‑151 (MQ=255)

                                            tGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGatc                                                                                                       >  3:394299/1‑151 (MQ=255)

                                             gCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGTGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGatc                                                                                                       <  1:1041684/150‑1 (MQ=255)

                                             gCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGatca                                                                                                      <  4:321741/151‑1 (MQ=255)

                                                     gACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGTTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAgg                                                                                              <  3:786121/151‑1 (MQ=255)

                                                     gACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAgg                                                                                              <  4:694452/151‑1 (MQ=255)

                                                        tGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCg                                                                                           >  6:740028/1‑151 (MQ=255)

                                                         gCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGc                                                                                            <  2:400597/149‑1 (MQ=255)

                                                         gCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGc                                                                                            <  5:1081594/149‑1 (MQ=255)

                                                          cTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTg                                                                                         >  2:1287607/1‑151 (MQ=255)

                                                            ttACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTg                                                                                         <  5:83168/149‑1 (MQ=255)

                                                            ttACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGa                                                                                        <  1:364600/150‑1 (MQ=255)

                                                                gTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGttt                                                                                   >  4:949061/1‑151 (MQ=255)

                                                                 tGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGttt                                                                                   >  7:1119286/1‑150 (MQ=255)

                                                                 tGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGttt                                                                                   >  8:635068/1‑150 (MQ=255)

                                                                 tGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGttt                                                                                   >  1:1661673/1‑150 (MQ=255)

                                                                  gcagcaAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAc                                                                                 >  5:261669/1‑151 (MQ=255)

                                                                  gcagcaAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAc                                                                                 >  5:1556484/1‑151 (MQ=255)

                                                                   caggaAAACGCTGCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCATATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAcc                                                                                <  7:1045081/151‑1 (MQ=255)

                                                                    agcaAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAccc                                                                               >  2:1643346/1‑151 (MQ=255)

                                                                    agcaAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGACAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTACACGGAGATCCCCGTCAAAGCTCACGATGTAGATCATCGGCAGGGCGTGACCTTTAccc                                                                               >  7:605923/1‑151 (MQ=255)

                                                                     gcaAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAcccc                                                                              >  1:749680/1‑150 (MQ=255)

                                                                      caAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCAc                                                                             >  7:24551/1‑151 (MQ=255)

                                                                      caAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCAc                                                                             >  2:87114/1‑151 (MQ=255)

                                                                       aaaaCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACt                                                                            >  1:1010895/1‑151 (MQ=255)

                                                                         aaCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTc                                                                          <  5:1003103/151‑1 (MQ=255)

                                                                          aCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCa                                                                         <  8:1199023/151‑1 (MQ=255)

                                                                             cTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCAt                                                                        >  8:1011773/1‑149 (MQ=255)

                                                                             cTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCAt                                                                        >  8:511821/1‑149 (MQ=255)

                                                                              tCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGt                                                                     >  6:1563134/1‑151 (MQ=255)

                                                                               gcAGTTTCTGGATCTCTGCAGGGATTTCGCTGGTATAAACTTCAGTTTCGTTTCCTTTGGTATCGAAGATCTTGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATGGGCAGGGCGTGACGTTTACCCACTTGATAGTc                                                                    <  2:609265/150‑1 (MQ=255)

                                                                                 aGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGt                                                                  >  4:772254/1‑151 (MQ=255)

                                                                                     tCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTaa                                                               >  5:1182373/1‑150 (MQ=255)

                                                                                      cTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGAATCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTaa                                                               >  3:198165/1‑149 (MQ=255)

                                                                                        ggAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTaaa                                                              <  6:495512/148‑1 (MQ=255)

                                                                                        ggAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGt                                                            <  3:148844/150‑1 (MQ=255)

                                                                                        ggAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTc                                                           <  8:1263938/151‑1 (MQ=255)

                                                                                         gAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCg                                                          >  8:1700512/1‑151 (MQ=255)

                                                                                           aCTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTg                                                        <  1:1559110/151‑1 (MQ=255)

                                                                                              ctGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgcgc                                                     >  8:1473582/1‑151 (MQ=255)

                                                                                                gCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgcg                                                      <  7:321960/148‑1 (MQ=255)

                                                                                                 cAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCgg                                                  <  3:1055199/151‑1 (MQ=255)

                                                                                                   gggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAg                                                <  2:1418820/151‑1 (MQ=255)

                                                                                                    ggATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGt                                               <  2:749338/151‑1 (MQ=255)

                                                                                                       tttCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGAt                                            >  7:1492960/1‑151 (MQ=255)

                                                                                                          cGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAGCCACTTCATAGTCGTTAATGTCGTGCGCCGGAGTGAtttt                                         >  1:1168058/1‑151 (MQ=255)

                                                                                                           gCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTc                                        >  2:298706/1‑151 (MQ=255)

                                                                                                              ggAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCa                                       <  1:843227/149‑1 (MQ=255)

                                                                                                              ggAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACg                                     <  2:151491/151‑1 (MQ=255)

                                                                                                               gAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGc                                    <  8:1414880/151‑1 (MQ=255)

                                                                                                               gAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGc                                    <  4:1041855/151‑1 (MQ=255)

                                                                                                                 aTAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGc                                    >  3:540608/1‑149 (MQ=255)

                                                                                                                   aaaCGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGcc                                <  7:1376565/151‑1 (MQ=255)

                                                                                                                       gTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTg                            <  7:1279879/151‑1 (MQ=255)

                                                                                                                        tCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGTTGTTGATCAACGGCAGGGCGTGACGTTTACCCACTTGATAGTCGTTAAAGTAGTGCGCCGGAGTGATTTTAACGGAGCCGGTgg                           >  5:75263/1‑150 (MQ=255)

                                                                                                                          aGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCt                         >  2:1121814/1‑151 (MQ=255)

                                                                                                                           gATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCtt                        <  1:459133/151‑1 (MQ=255)

                                                                                                                            aTTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCttt                       >  8:692813/1‑151 (MQ=255)

                                                                                                                              tCGTTACCTTTGGTATCGAACATCTGTTCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCTTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCtttt                      <  2:108531/150‑1 (MQ=255)

                                                                                                                              tCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCttttt                     >  5:662708/1‑151 (MQ=255)

                                                                                                                                 ttACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTAATAGTCGTTAAAGTCGTGCGCCGCAGTGATTTTCACGCAGCCGGTGCCTTTTTcc                   >  2:1559006/1‑150 (MQ=255)

                                                                                                                                       ttGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTc              <  7:857583/149‑1 (MQ=255)

                                                                                                                                         ggTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGCGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGc           <  6:1233483/150‑1 (MQ=255)

                                                                                                                                           tATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCgtg        >  3:1129364/1‑151 (MQ=255)

                                                                                                                                            aTCGAAGATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCgtg        <  6:617712/150‑1 (MQ=255)

                                                                                                                                            aTCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCgtgt       <  4:504561/151‑1 (MQ=255)

                                                                                                                                               gAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtcg    <  8:396669/151‑1 (MQ=255)

                                                                                                                                                 acatCTGGGCGCGTTCACGGATATCGCCGTCAAAGGGCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtcgtc  <  7:1198552/151‑1 (MQ=255)

AGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTC  >  NC_000913/4482753‑4483048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`