BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,501,500	C→T	L81L (CTG→TTG)	yjgZ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,501,500	0	C	T	95.5%	1488.6 / 40.4	465	L81L (CTG→TTG)	yjgZ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (14/7); new base T (219/224); total (233/232)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 465 total aligned reads displayed.

GCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGA  >  NC_000913/4501352‑4501646

  acacacaACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTgac                                                                                                                                                <  2:1569898/151‑1 (MQ=255)

   cacacaACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTgacg                                                                                                                                               >  7:1070972/1‑151 (MQ=255)

      acaacaATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTgacggac                                                                                                                                            <  2:1149447/151‑1 (MQ=255)

      acaacaATATCTCCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCGCTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTgacggac                                                                                                                                            >  8:383356/1‑151 (MQ=255)

            atatCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTg                                                                                                                                      >  5:1524407/1‑151 (MQ=255)

                 tGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGAcgcg                                                                                                                                 >  7:1395409/1‑151 (MQ=255)

                  gCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGAcgcg                                                                                                                                 <  3:1170390/150‑1 (MQ=255)

                  gCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGAc                                                                                                                                    <  7:922622/147‑1 (MQ=255)

                    gAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCt                                                                                                                              >  2:1233093/1‑151 (MQ=255)

                      gCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGc                                                                                                                            <  4:260641/151‑1 (MQ=255)

                       cGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGcc                                                                                                                           >  5:1247921/1‑151 (MQ=255)

                       cGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGcc                                                                                                                           <  8:704807/151‑1 (MQ=255)

                          gCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCgg                                                                                                                         >  1:886936/1‑150 (MQ=255)

                             aTCCGTCCGGTCGTTATGGGACGAGAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTg                                                                                                                     <  3:1328149/151‑1 (MQ=255)

                             aTCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAg                                                                                                                       <  5:907773/149‑1 (MQ=255)

                               cagtccgGTAGTTATGGGACGAAAGGCCTGGTTGTTCGCTGTTTCAGTTGTGTCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTTGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCTTCTGCCGTAGTgg                                                                                                                    <  1:1371283/148‑1 (MQ=255)

                                 gtccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCg                                                                                                                 <  2:1034946/151‑1 (MQ=255)

                                   ccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAGCCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCggc                                                                                                               >  2:912539/1‑150 (MQ=255)

                                   ccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCgga                                                                                                               <  7:143948/151‑1 (MQ=255)

                                   ccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCgga                                                                                                               <  6:1235500/151‑1 (MQ=255)

                                   ccgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCNGGTNCACTNGTGGCCGNGAACCGCGCNNCACAGATAATGAGNCTTCTNNNAACCGNAGTCTGNAGCCANANNNTTGGTTGACGNNCGTCCTGACGCGTCTGCCGGAGTGGCCgga                                                                                                               <  2:798826/151‑1 (MQ=255)

                                    cgGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCgg                                                                                                                >  2:1386007/1‑149 (MQ=255)

                                          ttATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACAGGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAgg                                                                                                        >  6:1644618/1‑151 (MQ=255)

                                           tATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAgg                                                                                                        <  7:384582/150‑1 (MQ=255)

                                             tGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTg                                                                                                     >  7:768621/1‑151 (MQ=255)

                                                gACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCt                                                                                                  <  1:1533355/151‑1 (MQ=255)

                                                  cGAAAGGCCTGGGTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGa                                                                                                <  7:625159/151‑1 (MQ=255)

                                                   gAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTg                                                                                                 >  7:276026/1‑149 (MQ=255)

                                                   gAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAg                                                                                               <  6:726166/151‑1 (MQ=255)

                                                    aaaGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGt                                                                                              >  1:1091572/1‑151 (MQ=255)

                                                     aaGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGCCGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGtt                                                                                             >  6:823957/1‑151 (MQ=255)

                                                        gCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGTCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACt                                                                                          <  4:1197481/151‑1 (MQ=255)

                                                         ccTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTg                                                                                         <  1:440483/151‑1 (MQ=255)

                                                         ccTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTg                                                                                         >  8:587058/1‑151 (MQ=255)

                                                          cTGGTTGTTCGCTGGTTCACTGGTGGCCGGGACCCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTCCTGc                                                                                        >  2:540242/1‑151 (MQ=255)

                                                           tGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGcc                                                                                       >  4:1615555/1‑151 (MQ=255)

                                                            ggTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCct                                                                                      <  3:1300313/151‑1 (MQ=255)

                                                            ggTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCct                                                                                      <  3:1424170/151‑1 (MQ=255)

                                                             gttgttCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCctc                                                                                     <  5:1448876/151‑1 (MQ=255)

                                                                gttCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTg                                                                                  >  5:49305/1‑151 (MQ=255)

                                                                  tCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGa                                                                                 <  8:1113728/150‑1 (MQ=255)

                                                                  tCGCTGGTGCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGa                                                                                 <  6:301186/150‑1 (MQ=255)

                                                                   cggtcgTTCACTGGTGGCCGGGAACCGCGAGTCACACATAATGATCCTTCTGGGAACCGGAGACTGGAGCCAGATGATTGGTTGCCGGACGTCCTGACGCGTCTGCCGGAGTGGCCTGATGAGACTTTGGCTGAGTTTCAGCCTCTTGAgg                                                                               >  1:963643/6‑151 (MQ=255)

                                                                    gCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAggg                                                                              >  8:281552/1‑151 (MQ=255)

                                                                          tCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTa                                                                         <  7:1628902/150‑1 (MQ=255)

                                                                          tCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTa                                                                         <  3:599924/150‑1 (MQ=255)

                                                                           cACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTAcc                                                                       >  4:69467/1‑151 (MQ=255)

                                                                            aCTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACct                                                                      <  8:1050993/151‑1 (MQ=255)

                                                                               ggtggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACcttct                                                                   <  4:871040/151‑1 (MQ=255)

                                                                               ggtggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACcttct                                                                   >  4:950780/1‑151 (MQ=255)

                                                                                  ggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCttcttc                                                                 <  5:1372821/150‑1 (MQ=255)

                                                                                  ggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCg                                                                <  1:168423/151‑1 (MQ=255)

                                                                                  ggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCg                                                                <  5:1327282/151‑1 (MQ=255)

                                                                                  ggCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCATCg                                                                >  1:684349/1‑151 (MQ=255)

                                                                                   gCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCgg                                                               >  6:1089670/1‑151 (MQ=255)

                                                                                          aCCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTg                                                        <  3:268056/151‑1 (MQ=255)

                                                                                           ccGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTg                                                        >  8:516083/1‑150 (MQ=255)

                                                                                            cgcgcgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGAt                                                      >  2:1458755/1‑151 (MQ=255)

                                                                                            cgcgcgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGAt                                                      <  3:71925/151‑1 (MQ=255)

                                                                                               gcgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATAcc                                                   <  8:185955/151‑1 (MQ=255)

                                                                                               gcgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATAcc                                                   >  2:1550464/1‑151 (MQ=255)

                                                                                                gggCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCt                                                  <  1:302229/150‑1 (MQ=255)

                                                                                                cgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCt                                                  >  4:455687/1‑151 (MQ=255)

                                                                                                cgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCt                                                  <  3:1037842/151‑1 (MQ=255)

                                                                                                cgGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCt                                                  >  6:367247/1‑151 (MQ=255)

                                                                                                 ggCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGTTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAATTGATACCTg                                                 <  8:1387299/151‑1 (MQ=255)

                                                                                                 ggCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGGCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTg                                                 <  7:1313975/151‑1 (MQ=255)

                                                                                                  gCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGc                                                >  4:1309358/1‑151 (MQ=255)

                                                                                                   cacaGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGcc                                               >  1:1001588/1‑151 (MQ=255)

                                                                                                   cacaGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGcc                                               <  1:593460/151‑1 (MQ=255)

                                                                                                   cacaGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGcc                                               >  2:1287006/1‑151 (MQ=255)

                                                                                                       gATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTc                                            >  5:1641710/1‑150 (MQ=255)

                                                                                                        aTAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACTTTCTTCGGGTAAGTGATACCTGCCGTCAg                                          <  8:735721/151‑1 (MQ=255)

                                                                                                        aTAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTc                                            >  5:1557439/1‑149 (MQ=255)

                                                                                                        aTAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGCCGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTc                                            >  8:197993/1‑149 (MQ=255)

                                                                                                          aaTGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAgg                                         <  6:1452614/150‑1 (MQ=255)

                                                                                                           aTGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGgtg                                       <  5:847135/151‑1 (MQ=255)

                                                                                                            tGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGgtgt                                      >  4:177628/1‑151 (MQ=255)

                                                                                                             gAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGtt                                     <  5:801192/151‑1 (MQ=255)

                                                                                                                ccTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTtgt                                  >  6:422101/1‑151 (MQ=255)

                                                                                                                  ttCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGc                                >  4:1174601/1‑151 (MQ=255)

                                                                                                                   tCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGc                                <  8:1580632/150‑1 (MQ=255)

                                                                                                                   tCTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGc                                <  7:558080/150‑1 (MQ=255)

                                                                                                                    cTGGGAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCAc                              >  3:151057/1‑151 (MQ=255)

                                                                                                                      gggAACCGGAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCAc                              <  4:1074570/149‑1 (MQ=255)

                                                                                                                      gggAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCa                               <  5:285989/148‑1 (MQ=255)

                                                                                                                       ggAACCGCAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCgg                           <  2:233636/151‑1 (MQ=255)

                                                                                                                        gAACCGCAGTCTGGAGCCACATGCTTGGTGGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCggg                          <  2:71261/151‑1 (MQ=255)

                                                                                                                         aaCCGCAGTCTGGAGCCACATGCTTGGTAGAAGCACGTCCTGACGCGTCTGCCGGAGTGGCCGGACGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGCTAAGTGATACATGCCGTCAGGTGTTTCTGCACCGGGc                         >  4:1369814/1‑151 (MQ=255)

                                                                                                                              cAGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATaa                    <  1:1265832/151‑1 (MQ=255)

                                                                                                                               aGTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAAc                   >  3:437364/1‑151 (MQ=255)

                                                                                                                                gTCTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGATTGTGCACCGGGCCATAAcc                  >  1:227596/1‑151 (MQ=255)

                                                                                                                                  cTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTg                >  6:971071/1‑151 (MQ=255)

                                                                                                                                  cTGGAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTg                >  8:932506/1‑151 (MQ=255)

                                                                                                                                    ggAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCt                 <  8:1301501/148‑1 (MQ=255)

                                                                                                                                    ggAGCCACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTCAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGc               <  1:477152/150‑1 (MQ=255)

                                                                                                                                        ccACATGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGATGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGCTGTTTGTGCACCGGGCCATAACCTGCAGTTg          >  2:418905/1‑151 (MQ=255)

                                                                                                                                         cacaTGCTTGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTgg         <  3:965430/151‑1 (MQ=255)

                                                                                                                                                 tGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCCTAACCTGCAGTTGGGAATTgc  >  3:850527/1‑149 (MQ=255)

                                                                                                                                                 tGGTTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGa  >  3:1094151/1‑150 (MQ=255)

GCACACACAACAATATCTGCGAGCGTGCTATCCGTCCGGTCGTTATGGGACGAAAGGCCTGGTTGTTCGCTGGTTCACTGGTGGCCGGGAACCGCGCGGCACAGATAATGAGCCTTCTGGGAACCGCAGTCTGGAGCCACATGCTTGGCTGACGGACGTCCTGACGCGTCTGCCGGAGTGGCCGGAGGAGAGGTTGGCTGAGTTACTGCCTCTTGAGGGCTTTACCTTCTTCGGGTAAGTGATACCTGCCGTCAGGTGTTTGTGCACCGGGCCATAACCTGCAGTTGGGAATTGA  >  NC_000913/4501352‑4501646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`