Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,524,401 C→T G204S (GGC→AGC)  yjhH ← putative lyase/synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,524,4010CT96.0% 1303.2 / 26.1 405G204S (GGC→AGC) yjhHputative lyase/synthase
Reads supporting (aligned to +/- strand):  ref base C (7/9);  new base T (193/191);  total (202/203)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 416 total aligned reads displayed.

GTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAG  >  NC_000913/4524251‑4524548
                                                                                                                                                      |                                                                                                                                                   
gtgtTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCgccgcc                                                                                                                                                     >  1:716532/1‑151 (MQ=255)
       gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCagcagc                                                                                                                                              >  2:246133/1‑151 (MQ=255)
       gAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCagcagc                                                                                                                                              <  5:690442/151‑1 (MQ=255)
        aGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCCGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAgcagca                                                                                                                                             <  2:915564/151‑1 (MQ=255)
        aGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCagcagc                                                                                                                                              >  6:1246118/1‑150 (MQ=255)
          ggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATc                                                                                                                                           <  1:368612/151‑1 (MQ=255)
          ggCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATc                                                                                                                                           >  3:1600756/1‑151 (MQ=255)
           gCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCg                                                                                                                                          <  8:1536659/151‑1 (MQ=255)
               aaaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTAtt                                                                                                                                      >  7:1488362/1‑151 (MQ=255)
               aaaTAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTAt                                                                                                                                       <  7:709384/150‑1 (MQ=255)
                  tAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTc                                                                                                                                     >  5:228048/1‑149 (MQ=255)
                   aGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGc                                                                                                                                  >  4:930053/1‑151 (MQ=255)
                     cGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCaa                                                                                                                                <  6:1595154/151‑1 (MQ=255)
                       ggCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAAt                                                                                                                              <  5:997938/151‑1 (MQ=255)
                        gCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATg                                                                                                                             <  7:1239480/151‑1 (MQ=255)
                         cAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATg                                                                                                                             >  7:985286/1‑150 (MQ=255)
                          aGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGat                                                                                                                           >  8:420538/1‑151 (MQ=255)
                          aGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGCTAGATGCCGACGGAGAGTACCGGAGCAAAGTTAGCGCTGGCCGATATCGCACCGTCGCCGCTCAGCAACATCGTAATCAGAAAATGa                                                                                                                            >  6:226417/1‑150 (MQ=255)
                              gtagtaGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGatcatc                                                                                                                       >  6:1659832/1‑151 (MQ=255)
                               tagtagTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCAACGTATTCAGCAAATGATCAACg                                                                                                                      >  2:598937/1‑151 (MQ=255)
                                agtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGt                                                                                                                     <  7:1558901/151‑1 (MQ=255)
                                 gtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGt                                                                                                                     >  4:1022239/1‑150 (MQ=255)
                                 gtagtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTa                                                                                                                    <  1:440033/151‑1 (MQ=255)
                                   agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                                                                   <  4:1527165/150‑1 (MQ=255)
                                   agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                                                                   <  2:1393511/150‑1 (MQ=255)
                                   agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTaa                                                                                                                   <  1:1162879/150‑1 (MQ=255)
                                   agtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAc                                                                                                                  >  2:1097611/1‑151 (MQ=255)
                                    gtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAcc                                                                                                                 >  7:654890/1‑151 (MQ=255)
                                    gtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAcc                                                                                                                 <  7:1306413/151‑1 (MQ=255)
                                    gtTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAAcc                                                                                                                 >  3:877867/1‑151 (MQ=255)
                                      tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                                                                <  2:488371/150‑1 (MQ=255)
                                      tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                                                                <  8:1294949/150‑1 (MQ=255)
                                      tttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                                                                <  1:1103713/150‑1 (MQ=255)
                                      gttttATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCg                                                                                                                <  1:1520237/149‑1 (MQ=255)
                                           aTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGaa                                                                                                           <  1:274416/150‑1 (MQ=255)
                                           aTTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAAt                                                                                                          <  7:596784/151‑1 (MQ=255)
                                                  gTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGaaa                                                                                                   <  2:284408/151‑1 (MQ=255)
                                                    cGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAac                                                                                                 >  2:1034072/1‑151 (MQ=255)
                                                     gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                                                                >  6:1553607/1‑151 (MQ=255)
                                                     gCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacg                                                                                                <  4:128089/151‑1 (MQ=255)
                                                      cAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgc                                                                                               >  8:522345/1‑150 (MQ=255)
                                                       aGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgac                                                                                              >  5:1576521/1‑151 (MQ=255)
                                                       aGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgac                                                                                              >  5:1052825/1‑151 (MQ=255)
                                                       aGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAacgac                                                                                              >  7:1663471/1‑151 (MQ=255)
                                                           gTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGc                                                                                          >  3:1330307/1‑151 (MQ=255)
                                                             cGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGt                                                                                        >  3:1213207/1‑151 (MQ=255)
                                                             cGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCAACGTAACCGCAGAATACCGAAAACGACGGGCGt                                                                                        <  6:1063196/151‑1 (MQ=255)
                                                                 aGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCg                                                                                    >  4:629850/1‑151 (MQ=255)
                                                                 aGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCg                                                                                    >  6:690695/1‑151 (MQ=255)
                                                                    tCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACt                                                                                 >  7:848473/1‑151 (MQ=255)
                                                                          tCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtg                                                                           >  8:352785/1‑151 (MQ=255)
                                                                            aCGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACtgtg                                                                         <  1:1210750/151‑1 (MQ=255)
                                                                               ccAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGCCTTAACTGTGTTg                                                                      >  1:1308634/1‑151 (MQ=255)
                                                                               ccAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                                                      >  6:736329/1‑151 (MQ=255)
                                                                                cAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                                                      >  6:441956/1‑150 (MQ=255)
                                                                                cAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTg                                                                      >  5:393437/1‑150 (MQ=255)
                                                                                cAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGa                                                                     <  2:1233196/151‑1 (MQ=255)
                                                                                   gcgcGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGatc                                                                   >  3:1550005/1‑150 (MQ=255)
                                                                                      cgGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCg                                                               <  2:657249/151‑1 (MQ=255)
                                                                                       ggTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTCGCGGTGGCGGTTATCGCACCGGCGCCGCGCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGt                                                              <  1:1034001/151‑1 (MQ=255)
                                                                                       ggTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGt                                                              <  4:1613259/151‑1 (MQ=255)
                                                                                            aTGCCTACGGAGAGTTCCGGATCAAATTTAGCGCTGGCGGTTATAGCACCGTCGCCGCTCAGCAGCATCGTATTAATCAAATGATCATAGTAACCGCAGAATACCGAAAACGACGGGATTACCGACTTAACTGTTTTGATCATCGTACGCa                                                         <  1:1371228/151‑1 (MQ=255)
                                                                                             tGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCa                                                         >  3:769224/1‑150 (MQ=255)
                                                                                              gCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCaa                                                        <  1:748672/150‑1 (MQ=255)
                                                                                                cGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTg                                                     >  8:70253/1‑151 (MQ=255)
                                                                                                cGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTg                                                     <  2:960470/151‑1 (MQ=255)
                                                                                                cGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTg                                                     >  7:1382353/1‑151 (MQ=255)
                                                                                                cGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGGCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTg                                                     <  6:1428613/151‑1 (MQ=255)
                                                                                                 gACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTAGCGACTTAACTGTGTTGATCCTCGTACGCAAGTg                                                     >  2:1442516/1‑150 (MQ=255)
                                                                                                   cGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATGGCACCGTCGCGGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAATCGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGAcc                                                  <  1:533800/151‑1 (MQ=255)
                                                                                                      agagTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAAc                                               <  6:267548/151‑1 (MQ=255)
                                                                                                       gagTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACg                                              <  7:1098780/151‑1 (MQ=255)
                                                                                                        agTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGc                                             >  8:800638/1‑151 (MQ=255)
                                                                                                           tCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGt                                          >  1:181904/1‑151 (MQ=255)
                                                                                                             cGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGtgg                                        >  1:801801/1‑149 (MQ=255)
                                                                                                              ggAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCg                                        >  3:209460/1‑150 (MQ=255)
                                                                                                              ggAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGa                                       <  5:847629/151‑1 (MQ=255)
                                                                                                              ggAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGa                                       <  2:1328070/151‑1 (MQ=255)
                                                                                                               gAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGAt                                      <  5:13042/151‑1 (MQ=255)
                                                                                                                aGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATg                                     >  5:1231350/1‑151 (MQ=255)
                                                                                                                aGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATg                                     <  3:531119/151‑1 (MQ=255)
                                                                                                                  cAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAACCGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGgt                                   <  2:164763/151‑1 (MQ=255)
                                                                                                                  cAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGgt                                   <  4:474825/151‑1 (MQ=255)
                                                                                                                         aGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCttt                             <  1:452156/150‑1 (MQ=255)
                                                                                                                         aGCGCTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCttt                             <  4:988914/150‑1 (MQ=255)
                                                                                                                          gcgcTGGCGGTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTg                            >  7:416196/1‑150 (MQ=255)
                                                                                                                                  ggTTATCGCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACg                   >  8:1071814/1‑151 (MQ=255)
                                                                                                                                     tATCGAACCTTCGCCGCTCATCTTCATCGTATTCAGCAAATGATCATCGTAACCTGAGAATACCGAAAACGACTGGGGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGTTGGTGTCTTTGATGCCAACGat                 <  6:1380925/150‑1 (MQ=255)
                                                                                                                                      aTCGCACCGGCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGatat               <  3:685424/151‑1 (MQ=255)
                                                                                                                                         gCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATtct            <  4:657641/151‑1 (MQ=255)
                                                                                                                                         gCACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATtct            <  8:282/151‑1 (MQ=255)
                                                                                                                                          cACCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATtctc           >  4:241059/1‑151 (MQ=255)
                                                                                                                                           aCCGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCg          >  3:365415/1‑151 (MQ=255)
                                                                                                                                             cGTCGCCGCTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGtt        >  4:338420/1‑151 (MQ=255)
                                                                                                                                              ggcgccgcTCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGttt       <  7:33887/149‑1 (MQ=255)
                                                                                                                                                cgccgcCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTg     <  6:393270/151‑1 (MQ=255)
                                                                                                                                                ccccgctCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTg     <  3:1662034/149‑1 (MQ=255)
                                                                                                                                                 gccgctCAGCAGCATCGTATTCAGCAAATGATCATCGTACCCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGc    <  5:528231/151‑1 (MQ=255)
                                                                                                                                                  ccgctCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGc    >  7:1080266/1‑150 (MQ=255)
                                                                                                                                                   cgctCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGc    >  8:119445/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GTGTTTCGAGGGCGTAAATAGCGGGCAGTTGTAGTAGTTTTTTATTCAGCGTCGCAGCGGTCGCCAGATCGCCTTCACGCCAGGCGCGGTAGATGCCGACGGAGAGTTCCGGAGCAAAGTTAGCGCTGGCGGTTATCGCACCGTCGCCGCCCAGCAGCATCGTATTCAGCAAATGATCATCGTAACCGCAGAATACCGAAAACGACGGGCGTACCGACTTAACTGTGTTGATCATCGTACGCAAGTGACCAACGCTGTCGATGGTGTCTTTGATGCCAACGATATTCTCGTTTTGCAG  >  NC_000913/4524251‑4524548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑