Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 340,202 G→A S13N (AGC→AAC)  yahI → carbamate kinase‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913340,2020GA97.9% 1616.1 / 13.5 466S13N (AGC→AAC) yahIcarbamate kinase‑like protein
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base A (221/234);  total (227/239)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 481 total aligned reads displayed.

GCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACG  >  NC_000913/340054‑340349
                                                                                                                                                    |                                                                                                                                                   
 ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACAt                                                                                                                                                  >  5:508854/1‑151 (MQ=255)
  cTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATTAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGGAACAACAtt                                                                                                                                                 >  6:1060655/1‑151 (MQ=255)
     aTCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTAt                                                                                                                                               <  7:1658948/150‑1 (MQ=255)
     aTCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTAt                                                                                                                                               <  2:1101590/150‑1 (MQ=255)
       ccGGCCTAGGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCa                                                                                                                                             <  4:1073184/150‑1 (MQ=255)
        cGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCaaa                                                                                                                                           <  8:1432206/151‑1 (MQ=255)
         ggCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAg                                                                                                                                          <  6:972577/151‑1 (MQ=255)
          gCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCaa                                                                                                                                            >  4:193500/1‑148 (MQ=255)
           ccTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGAt                                                                                                                                        <  8:818940/151‑1 (MQ=255)
              aCGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATaa                                                                                                                                      <  5:1214581/150‑1 (MQ=255)
               cGAGTGGCGCGAGGATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACg                                                                                                                                    >  7:1121874/1‑151 (MQ=255)
               cGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACg                                                                                                                                    >  7:1275168/1‑151 (MQ=255)
                gAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgc                                                                                                                                   >  4:11618/1‑151 (MQ=255)
                gAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgc                                                                                                                                   <  2:980485/151‑1 (MQ=255)
                  gTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgcca                                                                                                                                 <  3:19588/151‑1 (MQ=255)
                    ggCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgccagc                                                                                                                               >  3:1449509/1‑151 (MQ=255)
                    ggCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgccag                                                                                                                                <  2:244752/150‑1 (MQ=255)
                      cgcgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACgccagcc                                                                                                                              >  3:715523/1‑150 (MQ=255)
                       gcgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGccagccag                                                                                                                            <  8:938720/151‑1 (MQ=255)
                        cgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGt                                                                                                                           >  1:1080542/1‑151 (MQ=255)
                        cgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGt                                                                                                                           >  2:84738/1‑151 (MQ=255)
                        cgAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGt                                                                                                                           >  7:565677/1‑151 (MQ=255)
                          agaATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCg                                                                                                                         >  8:598226/1‑151 (MQ=255)
                          agaATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCg                                                                                                                         >  3:261306/1‑151 (MQ=255)
                           gaATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGa                                                                                                                        <  7:713297/151‑1 (MQ=255)
                               tGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTg                                                                                                                     <  5:128474/150‑1 (MQ=255)
                                  aGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGc                                                                                                                  >  3:1126705/1‑150 (MQ=255)
                                  aGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGCTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGc                                                                                                                  >  8:1187471/1‑150 (MQ=255)
                                   ggCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCAt                                                                                                                <  5:880763/151‑1 (MQ=255)
                                    gCCTGATAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCa                                                                                                              <  6:141302/151‑1 (MQ=255)
                                       tGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCagt                                                                                                            >  4:371530/1‑150 (MQ=255)
                                        gATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggc                                                                                                           <  5:1108873/151‑1 (MQ=255)
                                        gATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCagg                                                                                                            <  4:1578259/150‑1 (MQ=255)
                                         aTAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcg                                                                                                          >  6:1519745/1‑151 (MQ=255)
                                           aaGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcgga                                                                                                        <  6:179526/151‑1 (MQ=255)
                                           aaGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcgg                                                                                                         >  1:1309617/1‑150 (MQ=255)
                                            aGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCACATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcgga                                                                                                        >  4:674759/1‑150 (MQ=255)
                                             gCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcggaag                                                                                                      <  2:571169/151‑3 (MQ=255)
                                                 aGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcggaagcgg                                                                                                   <  8:1593955/150‑1 (MQ=255)
                                                  gcgcATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTg                                                                                                 <  1:210159/151‑1 (MQ=255)
                                                      aTCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGaa                                                                                               >  3:1286155/1‑149 (MQ=255)
                                                      aTCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAg                                                                                             <  5:344714/151‑1 (MQ=255)
                                                        cAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGcc                                                                                           >  8:34688/1‑151 (MQ=255)
                                                        aaGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGcc                                                                                           <  4:761530/150‑1 (MQ=255)
                                                           gCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTc                                                                                        <  3:1338898/151‑1 (MQ=255)
                                                            cAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCg                                                                                       >  4:995677/1‑151 (MQ=255)
                                                              gTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGcc                                                                                     <  3:553080/151‑1 (MQ=255)
                                                                cTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGGCg                                                                                    >  5:2923/1‑150 (MQ=255)
                                                                    cGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACg                                                                               <  5:490695/151‑1 (MQ=255)
                                                                    cGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACg                                                                               <  6:367058/151‑1 (MQ=255)
                                                                      ttGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGt                                                                             >  8:1099052/1‑151 (MQ=255)
                                                                        tgTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTTGTGGCAACAACATTATCAAAGATAACGCCAGCCAGACGATTGAGCATCAGCCGGAACCGGTGAAAGCCGTCGCCGATAGCGTGc                                                                           >  1:723900/2‑151 (MQ=255)
                                                                        ggTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTg                                                                            <  1:2910/150‑1 (MQ=255)
                                                                           cATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCCTCGCCGATACGGTGCTgg                                                                        >  1:1241876/1‑151 (MQ=255)
                                                                            aTAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTgg                                                                        >  4:929158/1‑150 (MQ=255)
                                                                            aTAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGACTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTgg                                                                        >  5:978756/1‑150 (MQ=255)
                                                                              aaCCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGaaa                                                                     <  6:471290/151‑1 (MQ=255)
                                                                               aCCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAAt                                                                    >  1:1382776/1‑151 (MQ=255)
                                                                               aCCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAAt                                                                    <  7:822210/151‑1 (MQ=255)
                                                                                ccccATCACCCTCTGTAGCGGACATAACAACATGAAAGAACTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATg                                                                   <  7:233330/151‑1 (MQ=255)
                                                                                 cccATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAACGCCGGCGCCGATACGGTGCTGGAAATGc                                                                  <  7:897829/151‑1 (MQ=255)
                                                                                  ccATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCt                                                                 >  5:158597/1‑151 (MQ=255)
                                                                                   cATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTg                                                                >  4:524561/1‑151 (MQ=255)
                                                                                      cccccTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCt                                                             <  7:7686/149‑1 (MQ=255)
                                                                                         ccTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTcc                                                          <  7:98129/151‑1 (MQ=255)
                                                                                            ctGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCCCCGATACGGTGCTGGAAATGCTGGCTTCCGAt                                                       >  6:1129021/1‑151 (MQ=255)
                                                                                              gTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGAtt                                                      <  2:406882/150‑1 (MQ=255)
                                                                                              gTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGAt                                                       <  8:1271230/149‑1 (MQ=255)
                                                                                              gTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGAt                                                       <  3:1205948/149‑1 (MQ=255)
                                                                                                    gACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACAt                                               <  3:987316/151‑1 (MQ=255)
                                                                                                     aCATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACAtt                                              <  7:1195537/151‑1 (MQ=255)
                                                                                                       aTAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATAAGGAGGAAGCGGTGAAAGCCGTAGACGATACCGTGCTTCAAATGCTGGCTTCCGATTACGACATtgt                                            >  6:68864/1‑151 (MQ=255)
                                                                                                        tAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGCTACGGTGCTGGAAATGCTGGCTTCCGATTACGACATtgtg                                           >  2:1374505/1‑151 (MQ=255)
                                                                                                         aacaacATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATtgtg                                           <  1:1534359/150‑1 (MQ=255)
                                                                                                          acaacaTGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCt                                         >  2:1622892/1‑151 (MQ=255)
                                                                                                          acaacaTGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGACGATTGACCAACAGGCGGAAGCGGTGAAAGCAGTCGGCGATAAGGTGCTGGAAATGCGGGCTTGCGATTACGACATTGTGCt                                         >  6:134126/1‑151 (MQ=255)
                                                                                                           caacaTGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTg                                        <  6:57432/151‑1 (MQ=255)
                                                                                                                tGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGTTGAAATCCGTCGCCGATACGGTTGTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGAccc                                    >  8:403378/1‑150 (MQ=255)
                                                                                                                 gAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCAc                                  <  6:9112/151‑1 (MQ=255)
                                                                                                                   acagagctggggTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACgg                                <  2:1308482/141‑1 (MQ=255)
                                                                                                                          tgtgGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACgg                          <  1:465703/150‑1 (MQ=255)
                                                                                                                          tgtgGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACgg                          <  2:670470/150‑1 (MQ=255)
                                                                                                                          tgtgGTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACg                           <  2:1457835/149‑1 (MQ=255)
                                                                                                                              gTCGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGc                     <  1:933139/151‑1 (MQ=255)
                                                                                                                               gcGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCa                    <  7:1432638/150‑1 (MQ=255)
                                                                                                                                cGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAg                   <  7:1555755/151‑1 (MQ=255)
                                                                                                                                cGTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAg                   <  8:850728/151‑1 (MQ=255)
                                                                                                                                 gTTGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAgg                  <  3:876689/151‑1 (MQ=255)
                                                                                                                                   tGCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGt                 <  2:787719/150‑1 (MQ=255)
                                                                                                                                    gCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTc                <  8:229414/150‑1 (MQ=255)
                                                                                                                                    gCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCg               >  4:1267272/1‑151 (MQ=255)
                                                                                                                                    gCCATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCg               >  7:1100198/1‑151 (MQ=255)
                                                                                                                                     ccATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCgg              >  7:435138/1‑151 (MQ=255)
                                                                                                                                      cATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCggg             >  3:1557800/1‑151 (MQ=255)
                                                                                                                                      cATTGGTGGCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCggg             >  1:1593115/1‑151 (MQ=255)
                                                                                                                                         tggtggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCt           <  4:286491/150‑1 (MQ=255)
                                                                                                                                           gtggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGa        <  5:177220/151‑1 (MQ=255)
                                                                                                                                            tggCAACAACATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAt       >  1:1039237/1‑151 (MQ=255)
                                                                                                                                                 acaacaTTATCAAAGATAACGCCAGCCAGTCGATTGATCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACg  <  1:645696/151‑1 (MQ=255)
                                                                                                                                                 acaacaTTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAAGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACg  <  5:144959/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACG  >  NC_000913/340054‑340349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: