Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 349,013 G→A G111E (GGA→GAA)  prpB → 2‑methylisocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,0130GA96.8% 1276.2 / 17.1 382G111E (GGA→GAA) prpB2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (7/5);  new base A (158/210);  total (167/215)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 382 total aligned reads displayed.

TCTACCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGA  >  NC_000913/348865‑349160
                                                                                                                                                    |                                                                                                                                                   
tCTACCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAAt                                                                                                                                                   >  5:1039310/1‑151 (MQ=255)
   aCCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTg                                                                                                                                                 <  6:580176/150‑1 (MQ=255)
   aCCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTg                                                                                                                                                 <  6:803520/150‑1 (MQ=255)
   aCCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTGTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTg                                                                                                                                                 <  4:68966/150‑1 (MQ=255)
        tgatgaTGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCata                                                                                                                                             <  1:68050/149‑1 (MQ=255)
        tgatgaTGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATACGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATAtt                                                                                                                                           >  5:93685/1‑151 (MQ=255)
         gatgatGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTg                                                                                                                                          >  7:1341509/1‑151 (MQ=255)
         gatgatGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTg                                                                                                                                          <  3:203710/151‑1 (MQ=255)
          atgatgTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGa                                                                                                                                         <  2:694552/151‑1 (MQ=255)
            gatgTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGa                                                                                                                                         >  2:1547726/1‑149 (MQ=255)
               gtgCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGAt                                                                                                                                     <  8:1298600/150‑1 (MQ=255)
                   tGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGt                                                                                                                                >  4:290377/1‑151 (MQ=255)
                    gACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGtt                                                                                                                               >  5:1408807/1‑151 (MQ=255)
                      ccGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTgg                                                                                                                             <  6:1374560/151‑1 (MQ=255)
                       cGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGt                                                                                                                            <  8:1448985/151‑1 (MQ=255)
                       cGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGt                                                                                                                            <  4:601055/151‑1 (MQ=255)
                        gACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTg                                                                                                                           >  2:1159148/1‑151 (MQ=255)
                          cATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCg                                                                                                                         >  7:1231929/1‑151 (MQ=255)
                          cATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCg                                                                                                                         >  3:1020797/1‑151 (MQ=255)
                              cGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAAc                                                                                                                     >  2:796649/1‑151 (MQ=255)
                              cGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAAc                                                                                                                     >  7:334393/1‑151 (MQ=255)
                                ccGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGc                                                                                                                   >  1:383730/1‑151 (MQ=255)
                                ccGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGc                                                                                                                   >  1:834883/1‑151 (MQ=255)
                                 cGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGATGATCAGGTTGGTGCGAAACGCt                                                                                                                  >  6:1253948/1‑151 (MQ=255)
                                   tATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGc                                                                                                                >  7:784395/1‑151 (MQ=255)
                                   tATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGc                                                                                                                >  4:1549545/1‑151 (MQ=255)
                                    aTCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGc                                                                                                                <  6:1154336/150‑1 (MQ=255)
                                    aTCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCg                                                                                                               >  6:1660234/1‑151 (MQ=255)
                                      cACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGt                                                                                                             >  1:1084106/1‑151 (MQ=255)
                                             gTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCTGAATTGCATATTGAACATCAGGTTCGTGCGAAACGCTGCGGTCATCGTc                                                                                                      >  4:1113799/1‑151 (MQ=255)
                                                    cgctgccgctgcTGGTGGATGCTGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATaa                                                                                               <  2:1622102/151‑1 (MQ=255)
                                                    cgctgccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATa                                                                                                <  4:1306842/150‑1 (MQ=255)
                                                      ctgccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGAGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATaa                                                                                               >  1:1036118/1‑149 (MQ=255)
                                                        gccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAg                                                                                             <  8:506076/149‑1 (MQ=255)
                                                        gccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGc                                                                                            <  3:512241/150‑1 (MQ=255)
                                                         ccgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGa                                                                                          <  3:672356/151‑1 (MQ=255)
                                                          cgctgcTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGAt                                                                                         >  5:32859/1‑151 (MQ=255)
                                                           gctgctGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATc                                                                                        >  1:1537947/1‑151 (MQ=255)
                                                           gctgctGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCGTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGAGATc                                                                                        <  7:105457/151‑1 (MQ=255)
                                                              gctgGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAACCGCTGCGGTCATCGTCCGAATAAAGCGATCGtc                                                                                     >  6:629405/1‑151 (MQ=255)
                                                              gctgGTGGATGCGGATATCGGTTTTGGTGCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGtc                                                                                     <  4:8054/151‑1 (MQ=255)
                                                               ctgGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTg                                                                                                                           <  5:193063/112‑1 (MQ=255)
                                                               ctgGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTg                                                                                                                           >  1:193910/1‑112 (MQ=255)
                                                                 ggtggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCg                                                                                  <  7:432239/151‑1 (MQ=255)
                                                                 ggtggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCg                                                                                  <  4:1411761/151‑1 (MQ=255)
                                                                    ggATGGGGATATCGGTTGTGGTTCTTCGGCCTTTAACGTGGCGCGCACCTTTAAATCGATGATTAAATGCGTTGCGGCAGAATTGCATATTGAAGATCAGGTTGGGGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGaaa                                                                               <  5:11435/151‑1 (MQ=255)
                                                                    ggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGaaa                                                                               <  2:951297/151‑1 (MQ=255)
                                                                    ggATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGaa                                                                                >  3:790575/1‑150 (MQ=255)
                                                                         cGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAaaga                                                                             >  6:714338/1‑148 (MQ=255)
                                                                          ggATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAagag                                                                         <  7:1125028/151‑1 (MQ=255)
                                                                          ggATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAagag                                                                         <  8:831029/151‑1 (MQ=255)
                                                                                cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAtggtg                                                                   >  8:368214/1‑151 (MQ=255)
                                                                                cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAtggt                                                                    <  5:810324/150‑1 (MQ=255)
                                                                                  gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAtggtgg                                                                  >  7:260553/1‑150 (MQ=255)
                                                                                     ttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatc                                                               <  2:1294966/150‑1 (MQ=255)
                                                                                        gTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatcggat                                                           <  2:1124607/151‑1 (MQ=255)
                                                                                          tcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatcggatc                                                          <  4:138180/150‑1 (MQ=255)
                                                                                           cttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcg                                                        >  2:32076/1‑151 (MQ=255)
                                                                                             tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatcggatca                                                         <  6:1505467/148‑2 (MQ=255)
                                                                                             tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGGCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgc                                                       <  5:698437/150‑1 (MQ=255)
                                                                                             tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGGGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATcc                                                         <  6:862483/148‑1 (MQ=255)
                                                                                              cGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgc                                                     <  4:512852/151‑1 (MQ=255)
                                                                                              cGGCCGTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcg                                                      <  5:1533197/150‑1 (MQ=255)
                                                                                               ggCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgcg                                                    >  1:1315229/1‑151 (MQ=255)
                                                                                               ggCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgcgcg                                                    >  7:1420481/1‑151 (MQ=255)
                                                                                                 ccTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGAgaggc                                                  >  5:1573060/1‑151 (MQ=255)
                                                                                                  cTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCgcggcg                                                 >  6:1303358/1‑151 (MQ=255)
                                                                                                    ttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGAGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGt                                               >  7:651029/1‑151 (MQ=255)
                                                                                                        cGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGAt                                           <  1:1474175/151‑1 (MQ=255)
                                                                                                           ggCGCGCACCGTGAAATCTATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCg                                        <  2:461026/151‑1 (MQ=255)
                                                                                                           ggCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCg                                        >  5:530230/1‑151 (MQ=255)
                                                                                                            gcgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGGGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGa                                       <  7:1462408/151‑1 (MQ=255)
                                                                                                              gcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaaa                                     <  7:587289/151‑1 (MQ=255)
                                                                                                                gcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                   >  7:917632/1‑151 (MQ=255)
                                                                                                                gcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                   >  2:544118/1‑151 (MQ=255)
                                                                                                                    cGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGAt                               >  1:557079/1‑151 (MQ=255)
                                                                                                                    cGTGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGAt                               >  3:927321/1‑151 (MQ=255)
                                                                                                                      tGAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGACAACCGCTcc                             >  4:1036064/1‑151 (MQ=255)
                                                                                                                       gAAATCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCt                            <  3:1544620/151‑1 (MQ=255)
                                                                                                                        aaaTCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCt                            <  1:1217941/150‑1 (MQ=255)
                                                                                                                          aTCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAt                         <  2:1059515/151‑1 (MQ=255)
                                                                                                                          aTCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAt                         <  6:353452/151‑1 (MQ=255)
                                                                                                                           tCGATGATTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                        >  8:45895/1‑151 (MQ=255)
                                                                                                                             gatgatTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                      <  8:115146/151‑1 (MQ=255)
                                                                                                                             gatgatTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                      <  8:284254/151‑1 (MQ=255)
                                                                                                                             gatgatTAAAGCCGGTGCGGCAGAATNGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTTGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                      <  8:107339/151‑1 (MQ=255)
                                                                                                                             gatgatTAAAGCCGGCGCGGCAGAATTGCATATTGAAGATCAGGTGGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCTATCGTCTCGAAAGAAGAGATGGTGCATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                      <  8:1237382/151‑1 (MQ=255)
                                                                                                                              atgatTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                     >  3:1426060/1‑151 (MQ=255)
                                                                                                                                 atTAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGa                  <  8:238065/151‑1 (MQ=255)
                                                                                                                                   tAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGAt                 <  3:1158490/150‑1 (MQ=255)
                                                                                                                                   tAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGAt                 <  6:48029/150‑1 (MQ=255)
                                                                                                                                   tAAAGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATc                >  6:1062322/1‑151 (MQ=255)
                                                                                                                                    aaaGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATc                >  7:1651049/1‑150 (MQ=255)
                                                                                                                                    aaaGCCGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCa               <  8:661426/151‑1 (MQ=255)
                                                                                                                                       gCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATgg            <  3:1311210/151‑1 (MQ=255)
                                                                                                                                        ccGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgc           <  1:231018/151‑1 (MQ=255)
                                                                                                                                         cGGTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcg          <  1:975218/151‑1 (MQ=255)
                                                                                                                                          ggTGCGGCAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcgc         >  6:53131/1‑151 (MQ=255)
                                                                                                                                              cGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAc     >  4:319907/1‑151 (MQ=255)
                                                                                                                                                 cAGAATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCg   >  8:1588497/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCTACCCTTGATGATGTGCTGACCGACATTCGCCGTATCACCGACGTTTGTTCGCTGCCGCTGCTGGTGGATGCGGATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGA  >  NC_000913/348865‑349160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑