Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 358,484 G→A S69S (AGC→AGT)  cynR ← transcriptional activator of cyn operon; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913358,4840GA96.2% 1372.0 / 25.9 423S69S (AGC→AGT) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (10/6);  new base A (248/156);  total (260/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

Only 100 of 423 total aligned reads displayed.

TGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCA  >  NC_000913/358336‑358631
                                                                                                                                                    |                                                                                                                                                   
  ggATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTgg                                                                                                                                                 <  5:1039077/151‑1 (MQ=255)
   gATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGc                                                                                                                                                >  4:1400747/1‑151 (MQ=255)
   gATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGc                                                                                                                                                >  3:872233/1‑151 (MQ=255)
     tAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGt                                                                                                                                              >  1:770388/1‑151 (MQ=255)
      aGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGt                                                                                                                                              <  7:389976/150‑1 (MQ=255)
        cgcgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACt                                                                                                                                           <  2:737803/151‑1 (MQ=255)
        cgcgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACt                                                                                                                                           >  8:1552060/1‑151 (MQ=255)
          cgcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCCTAAAGGTGGGGGTGACGGCGATAAGCAGCGATCCTCGCGTCAGATCCGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTg                                                                                                                                          >  7:962916/1‑150 (MQ=255)
           gcATAGACATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGGGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTc                                                                                                                                        <  7:1561625/151‑1 (MQ=255)
           gcATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTc                                                                                                                                        <  5:525360/151‑1 (MQ=255)
               aGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCa                                                                                                                                    <  6:218781/151‑1 (MQ=255)
                   aTCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAAACGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACt                                                                                                                                >  8:32844/1‑151 (MQ=255)
                       gCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTtctc                                                                                                                            >  3:1520088/1‑151 (MQ=255)
                        ccATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTcc                                                                                                                           >  8:243402/1‑151 (MQ=255)
                         cATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCt                                                                                                                          >  8:1685414/1‑151 (MQ=255)
                         cATTAAGGGGCCGATAAAGTAGCTCGTAAAGGGGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCt                                                                                                                          <  5:890881/151‑1 (MQ=255)
                          aTTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTg                                                                                                                         <  2:290516/151‑1 (MQ=255)
                           ttAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGc                                                                                                                        >  7:258220/1‑151 (MQ=255)
                             aaGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTCGCGTACTGTCGCCAGACTTCTGCTGCAt                                                                                                                      >  1:567266/1‑151 (MQ=255)
                                gggCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATc                                                                                                                     >  6:534636/1‑149 (MQ=255)
                                 ggCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGt                                                                                                                  <  8:719888/151‑1 (MQ=255)
                                  gCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTg                                                                                                                 <  7:522539/151‑1 (MQ=255)
                                      aTAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGAc                                                                                                             >  6:1301333/1‑151 (MQ=255)
                                        aaaGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACg                                                                                                            <  2:814134/150‑1 (MQ=255)
                                           gTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATc                                                                                                        <  1:686697/151‑1 (MQ=255)
                                            tAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCg                                                                                                       >  5:186327/1‑151 (MQ=255)
                                              gCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGtt                                                                                                     <  8:1369630/151‑1 (MQ=255)
                                               cTCGTAAACGAGGGGGTGACCCCGAAACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGATTACACGCCCCCAGTTCCTGTAACCCCCGACTGGCGTACTCTCCCCAGACTTCTCCTGCATCAGTGAGACGAATCCTTc                                                                                                    >  3:949579/1‑151 (MQ=255)
                                                  gTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGcc                                                                                                 >  6:891780/1‑151 (MQ=255)
                                                  gTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGcc                                                                                                 >  5:1296262/1‑151 (MQ=255)
                                                    aaaGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGccc                                                                                                <  2:1568959/150‑1 (MQ=255)
                                                    aaaGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCg                                                                                               <  2:38657/151‑1 (MQ=255)
                                                     aaGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGc                                                                                              >  1:1055121/1‑151 (MQ=255)
                                                      aGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCt                                                                                             >  2:957757/1‑151 (MQ=255)
                                                          gggggTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACg                                                                                          <  1:820986/150‑1 (MQ=255)
                                                            gggTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTc                                                                                       <  8:77240/151‑1 (MQ=255)
                                                              gTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGCCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCa                                                                                      >  6:9807/1‑150 (MQ=255)
                                                              gTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCa                                                                                      >  8:549365/1‑150 (MQ=255)
                                                               tGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCaa                                                                                     >  3:1629436/1‑150 (MQ=255)
                                                                gACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCaaa                                                                                    <  2:181001/150‑1 (MQ=255)
                                                                gACGGCGATACGCAGCGATCCTCGCGGCAGATCGGCAACAGCAGGAATCGCCCGGTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGGCAAAc                                                                                   <  4:69146/151‑1 (MQ=255)
                                                                 aCGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACa                                                                                  <  7:1479980/151‑1 (MQ=255)
                                                                   ggCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGc                                                                                <  5:417691/151‑1 (MQ=255)
                                                                   ggCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGc                                                                                <  1:276163/151‑1 (MQ=255)
                                                                   ggCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGc                                                                                >  8:587319/1‑151 (MQ=255)
                                                                     cGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCgg                                                                              <  1:1299805/151‑1 (MQ=255)
                                                                      gATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGc                                                                             >  2:47849/1‑151 (MQ=255)
                                                                       aTACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGcc                                                                            >  2:1229868/1‑150 (MQ=255)
                                                                       aTACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGCTCAAACAGCGGc                                                                             >  2:31661/1‑150 (MQ=255)
                                                                        tACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGccc                                                                           >  7:661432/1‑149 (MQ=255)
                                                                           gCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGcc                                                                        <  4:171530/151‑1 (MQ=255)
                                                                           gCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGcc                                                                        >  7:1067299/1‑151 (MQ=255)
                                                                            cAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCt                                                                       <  6:1052465/151‑1 (MQ=255)
                                                                                gATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAAc                                                                   >  4:1111926/1‑151 (MQ=255)
                                                                                   ccTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAActc                                                                 <  1:152652/150‑1 (MQ=255)
                                                                                    cTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGCCTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCCCGCCTAAACtctc                                                               >  1:1155759/1‑151 (MQ=255)
                                                                                    cTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACtctc                                                               >  1:429021/1‑151 (MQ=255)
                                                                                     tCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTctcc                                                              >  5:304717/1‑151 (MQ=255)
                                                                                     gcgcgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTctcc                                                              <  5:113311/150‑1 (MQ=255)
                                                                                      cgcgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGTTACGGTCAAACAGCGGCACGCCTAAACTctcct                                                             >  3:1089239/1‑151 (MQ=255)
                                                                                      cgcgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTctcct                                                             >  3:513644/1‑151 (MQ=255)
                                                                                       gcgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTctcctc                                                            >  3:2160/1‑151 (MQ=255)
                                                                                        cgTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCctct                                                           <  4:31528/151‑1 (MQ=255)
                                                                                          gcAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTaa                                                         <  3:1269521/150‑1 (MQ=255)
                                                                                              aTCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTgg                                                     >  6:6937/1‑151 (MQ=255)
                                                                                              aTCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTgg                                                     >  6:1185042/1‑151 (MQ=255)
                                                                                              aTCGGCAACATCATGAATAGCCCGTTTACACGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGAGGCACGCCTAAACTCTCCTCTAACTgg                                                     >  4:1177884/1‑151 (MQ=255)
                                                                                                cGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCg                                                   >  5:1204784/1‑151 (MQ=255)
                                                                                                  gCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGa                                                  >  4:846916/1‑150 (MQ=255)
                                                                                                   cAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAAt                                                <  5:153111/151‑1 (MQ=255)
                                                                                                       atcatGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgc                                            >  2:1056648/1‑151 (MQ=255)
                                                                                                         catGAATCTCCCGTTTACCCGCCGCCAGTTCCTGTAACGCCCGACTGTCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCTCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgct                                           <  7:1215110/150‑1 (MQ=255)
                                                                                                         catGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgctg                                          >  3:729577/1‑151 (MQ=255)
                                                                                                         catGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATctgctg                                          <  4:1657715/151‑1 (MQ=255)
                                                                                                          atGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTgg                                         >  8:1203995/1‑151 (MQ=255)
                                                                                                          atGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCATCAAACTGGCGATTCTGCTgg                                         >  7:777899/1‑151 (MQ=255)
                                                                                                              aTCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGaaa                                     <  3:1391302/151‑1 (MQ=255)
                                                                                                                cGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAgc                                   >  7:871028/1‑151 (MQ=255)
                                                                                                                cGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTCGCGAATCTGCTGCGAAAgc                                   >  4:243568/1‑151 (MQ=255)
                                                                                                                   ccGTTTCCCCGCCCCCAGTTCCTGTAACCCCCGACTCGCCTACTCTCCCCAGACTTCTCCTCCATCAGTCACACGAATCGTACCCCCCCTACGGTCAAACAGCGGCACCCCTCAACTCTCCTCTAACAGCCGAATCTCCTCGCaaaccac                                 >  2:63485/1‑146 (MQ=255)
                                                                                                                   ccGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAgcgc                                 >  1:20166/1‑150 (MQ=255)
                                                                                                                    cGTTTACCCGCCCGCAGTTCCTGTAACGCCTGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAg                               <  1:926625/151‑1 (MQ=255)
                                                                                                                    cGTTTACCCGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAg                               <  8:1298322/151‑1 (MQ=255)
                                                                                                                          cccGCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTggg                         <  8:1497478/151‑1 (MQ=255)
                                                                                                                          ccagCCCCCAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTggg                         <  2:134826/148‑1 (MQ=255)
                                                                                                                              cccccAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGAc                     >  6:1534072/1‑151 (MQ=255)
                                                                                                                              cccccAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGAc                     <  6:1420694/151‑1 (MQ=255)
                                                                                                                                 ccAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTg                  >  2:1410247/1‑151 (MQ=255)
                                                                                                                                 ccAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTg                  >  3:59865/1‑151 (MQ=255)
                                                                                                                                  cAGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGc                 >  4:1539358/1‑151 (MQ=255)
                                                                                                                                   aGTTCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCa                <  5:619116/151‑1 (MQ=255)
                                                                                                                                     ttCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAAc              >  1:161982/1‑151 (MQ=255)
                                                                                                                                      tCCTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACg             >  2:1117550/1‑151 (MQ=255)
                                                                                                                                      tCCTGAAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAAc              <  7:1614548/150‑1 (MQ=255)
                                                                                                                                       ccTGTAACGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACg             <  4:534090/150‑1 (MQ=255)
                                                                                                                                            aaCGCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTg        <  8:1117280/150‑1 (MQ=255)
                                                                                                                                               gCCCGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcgg    <  5:467455/151‑1 (MQ=255)
                                                                                                                                                cccGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcggc   >  4:1315886/1‑151 (MQ=255)
                                                                                                                                                 ccGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTggcggc   >  3:145311/1‑150 (MQ=255)
                                                                                                                                                 ccGACTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCa  <  4:760390/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGGATAGCGCGCATAGAAATCCGCCATTAAGGGGCCGATAAAGTAGCTCGTAAAGGTGGGGGTGACGGCGATACGCAGCGATCCTCGCGTCAGATCGGCAACATCATGAATCGCCCGTTTACCCGCCCCCAGTTCCTGTAACGCCCGGCTGGCGTACTGTCGCCAGACTTCTCCTGCATCAGTGAGACGAATCGTTCGCCCGCTACGGTCAAACAGCGGCACGCCTAAACTCTCCTCTAACTGGCGAATCTGCTGGGAAAGCGCAGGTTGGGAGACGTGCAACGCACTGGCGGCA  >  NC_000913/358336‑358631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑