BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	362,730	G→A	G150G (GGC→GGT)	lacY ←	lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	362,730	0	G	A	97.6%	1379.1 / 12.1	427	G150G (GGC→GGT)	lacY	lactose permease
Reads supporting (aligned to +/- strand): ref base G (2/8); new base A (211/199); total (220/207)
Fisher's exact test for biased strand distribution p-value = 5.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 434 total aligned reads displayed.

CATTGGCAACCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTG  >  NC_000913/362582‑362877

cATTGGCAACCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAAcc                                                                                                                                                   >  1:588528/1‑151 (MQ=255)

      cAACCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGATCATGATGCCGACAATCGAGGCACACAGCGCCCAACCAacac                                                                                                                                              >  2:890668/1‑150 (MQ=255)

       aaCCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAg                                                                                                                                            <  2:1628951/151‑1 (MQ=255)

       aaCCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAg                                                                                                                                            <  4:467755/151‑1 (MQ=255)

        aCCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGc                                                                                                                                           >  6:711186/1‑151 (MQ=255)

        aCCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGc                                                                                                                                           <  5:667388/151‑1 (MQ=255)

         ccGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACCGcc                                                                                                                                          >  1:33452/1‑151 (MQ=255)

          cGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCa                                                                                                                                         <  4:1361803/151‑1 (MQ=255)

          cGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCa                                                                                                                                         <  4:772952/151‑1 (MQ=255)

            tGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGAGTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACAGAGCCaa                                                                                                                                        >  3:730985/1‑150 (MQ=255)

              gctgaagaGGGGGCATCCGTTTTGGCGAAAAAGATTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGGACACAGCGCCCAACCAACACAGCCAAACa                                                                                                                                     <  7:1411433/148‑1 (MQ=255)

              gCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCCGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAAc                                                                                                                                      >  2:1153834/1‑150 (MQ=255)

              gCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACa                                                                                                                                     <  5:937838/151‑1 (MQ=255)

               cAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACAt                                                                                                                                    <  6:519050/151‑1 (MQ=255)

                agaagaGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATc                                                                                                                                   >  7:1351248/1‑151 (MQ=255)

                 gaagaGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATcc                                                                                                                                  <  7:15655/151‑1 (MQ=255)

                   agagGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCcgc                                                                                                                                >  2:421551/1‑151 (MQ=255)

                            aTCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGAcc                                                                                                                        >  6:369335/1‑150 (MQ=255)

                              ccGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCaa                                                                                                                      >  5:358292/1‑150 (MQ=255)

                               cGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                                                                                    <  3:1398132/151‑1 (MQ=255)

                                 ttttGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                                                                                  >  4:897093/1‑151 (MQ=255)

                                  tttGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCGCACAGCGCCCACCCAACACAGCCAAACATCCCCGCGCGACCAAATTCg                                                                                                                 >  4:1098016/1‑151 (MQ=255)

                                  tttGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                                                                                  <  1:1116817/150‑1 (MQ=255)

                                   ttGGCGAAAAAGAGTNAAACGGCGANGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGNTTANTGATGGTGAACATGATNCCGACAANCGAGNCNCACAGCGNCCNNcnnnnn                                                                                                                                               >  3:1185104/1‑115 (MQ=255)

                                   ttGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGGACAGCCATAGCCCAGCCAGAATACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAAGACAGCCAAAGATCCGCGCGCGACCAAATTCg                                                                                                                 <  6:1288023/150‑1 (MQ=255)

                                     ggCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaaa                                                                                                              <  1:1162876/151‑1 (MQ=255)

                                        gAAAAAGAGTAAAACGGCGAGGATGAATGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAtt                                                                                                            >  3:875436/1‑150 (MQ=255)

                                               agTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                                                                                    >  8:689584/1‑151 (MQ=255)

                                                 tAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACgg                                                                                                  >  7:225883/1‑151 (MQ=255)

                                                 tAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAAACAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACgg                                                                                                  >  2:977645/1‑151 (MQ=255)

                                                     aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGa                                                                                              <  7:726533/151‑1 (MQ=255)

                                                     aCGGCGAGCATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAATCTGATTATTGATGGTGAACATGATGCCGACTATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGa                                                                                              <  1:1009188/151‑1 (MQ=255)

                                                      cGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGAc                                                                                             >  4:1171084/1‑151 (MQ=255)

                                                       ggCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACt                                                                                            >  3:1524995/1‑151 (MQ=255)

                                                           aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                                                                                        >  5:1375948/1‑151 (MQ=255)

                                                           aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                                                                                        <  7:1658271/151‑1 (MQ=255)

                                                             gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                                                                                      <  4:1492739/151‑1 (MQ=255)

                                                                gAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATa                                                                                   <  8:836573/151‑1 (MQ=255)

                                                                 aGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATa                                                                                   >  8:1090983/1‑150 (MQ=255)

                                                                    gCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt                                                                                <  2:212941/150‑1 (MQ=255)

                                                                    gCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATg                                                                               <  8:407954/151‑1 (MQ=255)

                                                                     cacaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGc                                                                              <  3:475677/151‑1 (MQ=255)

                                                                     aacaGCCAGAGACAATCCAGAAAACAAAATGATTATTCAAGGTCAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCCACCAAATAAGAAATTACTCCTACGGCAGACTATCTCAATAAATGc                                                                              >  1:1287410/2‑151 (MQ=255)

                                                                      acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc                                                                             >  7:388300/1‑151 (MQ=255)

                                                                      acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc                                                                             <  2:844675/151‑1 (MQ=255)

                                                                       caGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCct                                                                            <  7:443597/151‑1 (MQ=255)

                                                                         gCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct                                                                          >  6:761473/1‑151 (MQ=255)

                                                                          ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctct                                                                          >  6:920673/1‑150 (MQ=255)

                                                                           cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc                                                                        >  5:1615082/1‑151 (MQ=255)

                                                                            agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct                                                                       <  2:769379/151‑1 (MQ=255)

                                                                              agCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActcc                                                                     >  4:1617212/1‑149 (MQ=255)

                                                                               gCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct                                                                    >  3:169062/1‑151 (MQ=255)

                                                                                cccAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACACCCAAACATGCGCGCGCGACCAAATTCGAAATTACTGCGCCGGCTGACTTTCTCAATAAATGCCTCTActgctg                                                                   <  4:1474241/151‑1 (MQ=255)

                                                                                 ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTgg                                                                  >  3:1270112/1‑151 (MQ=255)

                                                                                  cagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGgc                                                                 >  5:283106/1‑151 (MQ=255)

                                                                                   agccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGgcg                                                                >  7:643517/1‑151 (MQ=255)

                                                                                   agccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGgcg                                                                <  6:1055438/151‑1 (MQ=255)

                                                                                     ccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCa                                                              <  5:1201567/151‑1 (MQ=255)

                                                                                      cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCACCCAACACCGCCAAACATCCGCGCGCGCCCAAATTCGAAATTACTGCGACGCCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCAc                                                             >  4:198108/1‑151 (MQ=255)

                                                                                      cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCACCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCAc                                                             >  6:834402/1‑151 (MQ=255)

                                                                                       agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCTAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGTCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCAcc                                                            <  2:1491566/151‑1 (MQ=255)

                                                                                        caaaaCAAACTGATTATTGATGCTGAACATGAAGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCg                                                           <  8:1002475/150‑1 (MQ=255)

                                                                                         aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGTACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCgg                                                          >  1:1399971/1‑151 (MQ=255)

                                                                                          aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGc                                                         <  6:638241/151‑1 (MQ=255)

                                                                                            acaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGt                                                       <  2:1308331/151‑1 (MQ=255)

                                                                                              aaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTa                                                     <  4:1298743/151‑1 (MQ=255)

                                                                                               aacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTaa                                                    <  8:360529/151‑1 (MQ=255)

                                                                                                  tGATTATTGATGGTGAACATGATGCCGAC‑ATCGAGGCACACAGCGCCCAACCACCACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAAc                                                 >  5:1455855/1‑150 (MQ=255)

                                                                                                   gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTaaaa                                                  <  7:1133886/149‑1 (MQ=255)

                                                                                                   gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAAc                                                 >  7:509907/1‑150 (MQ=255)

                                                                                                    attattGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACa                                                >  8:1192984/1‑150 (MQ=255)

                                                                                                      tattGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAAccacc                                             >  6:271395/1‑147 (MQ=255)

                                                                                                       attGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAg                                            <  8:1568013/151‑1 (MQ=255)

                                                                                                         tGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGcc                                          >  4:706618/1‑151 (MQ=255)

                                                                                                          gATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGcc                                          <  4:247972/150‑1 (MQ=255)

                                                                                                           aTGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCt                                         >  1:185095/1‑150 (MQ=255)

                                                                                                           aTGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTa                                        <  8:951875/151‑1 (MQ=255)

                                                                                                              gTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCt                                         >  6:302737/1‑147 (MQ=255)

                                                                                                               tGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGAt                                     >  1:776397/1‑150 (MQ=255)

                                                                                                               tGAACATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGAt                                     >  3:1100587/1‑150 (MQ=255)

                                                                                                                 aaCATGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATaa                                   >  6:1641138/1‑150 (MQ=255)

                                                                                                                 aaCAAGATGCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATaaa                                  <  8:529703/151‑1 (MQ=255)

                                                                                                                   cATGATGCCGCCAATGTACGCACACAGCGCCCAACCAACACAGCCAAACATCCTGGCGCGACGAAATTCGAAATTAATTCGACGGGTGACTTTCTCAATTAATGCCTGTACTTCTTGGGCTCCGGCGTTTAAACAAAAGCCTAGATAAATa                                <  3:1038311/151‑1 (MQ=255)

                                                                                                                    atgatgCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATac                               >  6:1568971/1‑151 (MQ=255)

                                                                                                                     tgatgCCGACAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATacc                              >  8:926431/1‑151 (MQ=255)

                                                                                                                       atgCCGACAATCGAGGCACACCGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATaccac                            >  4:549000/1‑151 (MQ=255)

                                                                                                                             aCAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACcaacaa                      <  2:571760/151‑1 (MQ=255)

                                                                                                                              cAATCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAAt                     <  8:1665136/151‑1 (MQ=255)

                                                                                                                               aaTCGAGGCACACAGCGCCCAACCAACAGAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATc                    <  7:873625/151‑1 (MQ=255)

                                                                                                                               aaTCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATc                    >  5:498252/1‑151 (MQ=255)

                                                                                                                                aTCGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCg                   <  3:268423/151‑1 (MQ=255)

                                                                                                                                  cGAGGCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGAt                 >  5:816878/1‑151 (MQ=255)

                                                                                                                                     ggCACACAGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCct              <  7:1480716/151‑1 (MQ=255)

                                                                                                                                      ccacacaGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCcta             <  6:143886/150‑1 (MQ=255)

                                                                                                                                       cacacaGCGCCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCctac            >  8:241303/1‑151 (MQ=255)

                                                                                                                                              cgcCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtg     <  1:152162/151‑1 (MQ=255)

                                                                                                                                               gcCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtgt    >  1:220763/1‑151 (MQ=255)

                                                                                                                                               gcCCAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtgt    <  6:977350/151‑1 (MQ=255)

                                                                                                                                                cccAACCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtgtt   >  7:1237073/1‑151 (MQ=255)

                                                                                                                                                 ccaaccaaCACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAAtgttg  >  8:14087/1‑151 (MQ=255)

CATTGGCAACCGTGGCAGAAGAGGGCGCATCCGTTTTGGCGAAAAAGAGTAAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCTGGCGCACCGGCGTTAAAACAAAAGCCTAGATAAATACCACCAACAATCGATCCTACTAAAATGTTG  >  NC_000913/362582‑362877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`