Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 365,307 G→A F333F (TTC→TTT)  lacZ ← beta‑D‑galactosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913365,3070GA96.2% 1413.8 / 28.7 427F333F (TTC→TTT) lacZbeta‑D‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (10/6);  new base A (221/190);  total (231/196)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 432 total aligned reads displayed.

GTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACC  >  NC_000913/365157‑365453
                                                                                                                                                      |                                                                                                                                                  
gttCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACAATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCgg                                                                                                                                                    >  6:636674/1‑151 (MQ=255)
     gCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACTCCTCTAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGaaaa                                                                                                                                                 <  2:762282/149‑1 (MQ=255)
        tcatcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGAc                                                                                                                                            >  3:1527173/1‑151 (MQ=255)
        tcatcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATACGCACCTCGCGAAAACCGAc                                                                                                                                            >  4:1233324/1‑151 (MQ=255)
         catcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGAc                                                                                                                                            >  5:1527023/1‑150 (MQ=255)
         catcaCCAGGATATAATGCACAATAGTCTGCTCATCCATTAAATGACCATCCACAGGATGAAGATCGTGACCGTTAACCCCTAGAATCACCAACGGCTTGCCTTTCAGCAAAAGAACACCATTTTCAATCCGCACCTCGCGaaaaaccac                                                                                                                                            >  2:383620/1‑145 (MQ=255)
          atcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACAt                                                                                                                                          >  3:598121/1‑151 (MQ=255)
          atcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACAt                                                                                                                                          >  3:985523/1‑151 (MQ=255)
            cagcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATc                                                                                                                                         <  5:13380/150‑1 (MQ=255)
             agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGc                                                                                                                                       <  8:1243730/151‑1 (MQ=255)
             agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGc                                                                                                                                       >  7:1241891/1‑151 (MQ=255)
              gcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGc                                                                                                                                       >  3:1024181/1‑150 (MQ=255)
              gcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCa                                                                                                                                      <  3:1164208/151‑1 (MQ=255)
                agGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGACTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAgg                                                                                                                                    >  2:855462/1‑151 (MQ=255)
                  gATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCt                                                                                                                                  <  3:909949/151‑1 (MQ=255)
                        cTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATAAGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCt                                                                                                                            >  1:1006238/1‑151 (MQ=255)
                        cTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCATCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCt                                                                                                                            >  8:1586134/1‑151 (MQ=255)
                          gCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGGAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGCCATCGCAGGCTTCTGCTTc                                                                                                                          >  6:664596/1‑151 (MQ=255)
                          gCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTc                                                                                                                          >  1:1113088/1‑151 (MQ=255)
                               aTCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATc                                                                                                                      >  4:1079599/1‑150 (MQ=255)
                                   tCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGt                                                                                                                 >  2:325342/1‑151 (MQ=255)
                                      gCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTg                                                                                                                <  3:730871/149‑1 (MQ=255)
                                      gCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGGTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGt                                                                                                                 <  5:215320/148‑1 (MQ=255)
                                       cTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCg                                                                                                             >  2:1356881/1‑151 (MQ=255)
                                        tCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGt                                                                                                            >  4:632953/1‑151 (MQ=255)
                                         cATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGt                                                                                                            <  4:1631142/150‑1 (MQ=255)
                                         cATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTc                                                                                                           <  4:15132/151‑1 (MQ=255)
                                         cATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTc                                                                                                           <  1:1400440/151‑1 (MQ=255)
                                          aTCCATGACCTGACCATGCAGAGGATGATGCTCGTGACTGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGGAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcg                                                                                                          <  6:898528/151‑1 (MQ=255)
                                          aTCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAAAGCCTCGAATCAGAAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcg                                                                                                          >  1:1525884/1‑151 (MQ=255)
                                           tCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                                         >  2:1679915/1‑151 (MQ=255)
                                              aTGACCTGGCCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGAAGAGCATTTTCAATCCGCACCTCGCGAAAACAGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcgg                                                                                                      >  6:813513/1‑151 (MQ=255)
                                               tgacctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgt                                                                                                     >  3:136408/1‑151 (MQ=255)
                                                gacctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCA‑TTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtgt                                                                                                   <  5:838029/151‑1 (MQ=255)
                                                  cctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGtgtg                                                                                                  >  7:798451/1‑151 (MQ=255)
                                                  cctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGtgtg                                                                                                  >  8:833724/1‑151 (MQ=255)
                                                     gaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAg                                                                                               <  3:281521/151‑1 (MQ=255)
                                                     gaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCCAAAACCCACATCGCAGGCTTCTGCTTCAATCAGCCTGCCGTCGGCGCTGTGc                                                                                                 >  5:1588357/1‑149 (MQ=255)
                                                       ccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGtt                                                                                             >  6:1177344/1‑151 (MQ=255)
                                                        cATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTc                                                                                            <  4:997920/151‑1 (MQ=255)
                                                           gCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAac                                                                                         >  4:334426/1‑151 (MQ=255)
                                                            cAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAacc                                                                                        >  7:95813/1‑151 (MQ=255)
                                                            cAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAacc                                                                                        >  2:521580/1‑151 (MQ=255)
                                                             agagGATGATGCTCGTGACGGTTAACGCCTCGAATGAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAacca                                                                                       <  2:1084875/151‑1 (MQ=255)
                                                                 gatgatGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGc                                                                                   >  5:313682/1‑151 (MQ=255)
                                                                  atgatgCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGc                                                                                   >  5:736021/1‑150 (MQ=255)
                                                                     atgCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGa                                                                               <  3:1244516/151‑1 (MQ=255)
                                                                       gCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGAt                                                                              <  1:854098/150‑1 (MQ=255)
                                                                       gCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGAt                                                                              <  3:229272/150‑1 (MQ=255)
                                                                          cGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATagag                                                                          <  5:1203648/151‑1 (MQ=255)
                                                                          cGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATaga                                                                           <  6:458350/150‑1 (MQ=255)
                                                                             gACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGAt                                                                        <  8:1461320/150‑1 (MQ=255)
                                                                                 gTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCggg                                                                   >  8:1127823/1‑151 (MQ=255)
                                                                                  ttAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCggg                                                                   >  4:817648/1‑150 (MQ=255)
                                                                                    aaCGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGAtt                                                                <  6:1341931/151‑1 (MQ=255)
                                                                                     aCGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGAttt                                                               <  4:1056774/151‑1 (MQ=255)
                                                                                      cGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTc                                                              <  8:669649/151‑1 (MQ=255)
                                                                                        ccTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCgg                                                            <  1:671808/151‑1 (MQ=255)
                                                                                        ccTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCgg                                                            >  3:1633186/1‑151 (MQ=255)
                                                                                           cGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgc                                                           <  5:56789/149‑1 (MQ=255)
                                                                                           cGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGgc                                                           <  1:355186/149‑1 (MQ=255)
                                                                                            gAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCt                                                        >  2:1017957/1‑151 (MQ=255)
                                                                                             aaTCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTc                                                       <  6:495250/151‑1 (MQ=255)
                                                                                                cAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCcac                                                    >  6:1632891/1‑151 (MQ=255)
                                                                                                 aGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCcaca                                                   <  2:28126/151‑1 (MQ=255)
                                                                                                  gCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGGGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAg                                                  <  1:1652789/151‑1 (MQ=255)
                                                                                                   cAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGt                                                 >  8:17631/1‑151 (MQ=255)
                                                                                                    aaCGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGtt                                                <  1:314768/151‑1 (MQ=255)
                                                                                                    aaCGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGtt                                                >  1:511523/1‑151 (MQ=255)
                                                                                                    aaCGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGtt                                                >  3:846985/1‑151 (MQ=255)
                                                                                                       ggCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCg                                             >  5:939559/1‑151 (MQ=255)
                                                                                                       ggCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCg                                             >  4:1360704/1‑151 (MQ=255)
                                                                                                           tGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGt                                          <  1:144178/150‑1 (MQ=255)
                                                                                                            gCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGttt                                        <  5:1243904/151‑1 (MQ=255)
                                                                                                             ccGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGtttt                                       <  7:469527/151‑1 (MQ=255)
                                                                                                              cGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTc                                      >  7:1589909/1‑151 (MQ=255)
                                                                                                               gTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCg                                     >  6:329626/1‑151 (MQ=255)
                                                                                                                 tCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGAc                                   >  3:314002/1‑151 (MQ=255)
                                                                                                                    gcagcagcagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGtt                                >  7:297993/1‑151 (MQ=255)
                                                                                                                       gcagcagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAg                             <  6:150865/151‑1 (MQ=255)
                                                                                                                        cagcagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTc                               >  7:1433231/1‑148 (MQ=255)
                                                                                                                           cagACCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGt                         >  5:471179/1‑151 (MQ=255)
                                                                                                                              aCCATTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAgt                      >  4:927601/1‑151 (MQ=255)
                                                                                                                                 aTTTTCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGtgtg                   <  3:483455/151‑1 (MQ=255)
                                                                                                                                   tttCAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGa                  <  4:1442236/150‑1 (MQ=255)
                                                                                                                                      cAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg              >  2:1525864/1‑151 (MQ=255)
                                                                                                                                      cAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgc               <  2:903010/150‑1 (MQ=255)
                                                                                                                                      cAATCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgc               <  7:507040/150‑1 (MQ=255)
                                                                                                                                       aaTCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGCTGTGCAGTTCAACCACCCCACGATAGAGATTCGCGATTTCGGCGCTCCACACTATCGGGTTTTCGACGTTCATACGAAGTGTGAcgcg              >  1:1172711/1‑150 (MQ=255)
                                                                                                                                         tCCGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGGTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATc           >  8:50948/1‑151 (MQ=255)
                                                                                                                                          ccGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCg          <  1:1265007/151‑1 (MQ=255)
                                                                                                                                          ccGCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGGGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATc           <  6:1083643/150‑1 (MQ=255)
                                                                                                                                            gCACCTCGCGACAACCGACAGCGCAGGCTTCTGCGTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTGCGGGTTTTCGACGTGCAGACGTAGTGTGACGCGATCGGc        <  6:228698/151‑1 (MQ=255)
                                                                                                                                            gCACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAAGCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGc        <  8:1192971/151‑1 (MQ=255)
                                                                                                                                             cACCTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCa       <  7:1166755/151‑1 (MQ=255)
                                                                                                                                               ccTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAt      >  7:1052377/1‑150 (MQ=255)
                                                                                                                                               ccTCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATa     <  7:557402/151‑1 (MQ=255)
                                                                                                                                                 tCGCGAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATGGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAac   >  3:1610881/1‑151 (MQ=255)
                                                                                                                                                  cgcgAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATaa    <  2:1462943/149‑1 (MQ=255)
                                                                                                                                                   gcgAAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAacc  >  8:298477/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
GTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACC  >  NC_000913/365157‑365453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑