Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 31,683 C→T N289N (AAC→AAT)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091331,6830CT97.0% 1353.8 / 17.7 406N289N (AAC→AAT) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (8/4);  new base T (199/195);  total (207/199)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 409 total aligned reads displayed.

GGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAC  >  NC_000913/31536‑31830
                                                                                                                                                   |                                                                                                                                                   
          acCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGgtc                                                                                                                                        <  5:1631946/151‑1 (MQ=255)
                 aCTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAt                                                                                                                                 >  6:540949/1‑151 (MQ=255)
                    ccATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTg                                                                                                                               <  8:1273815/150‑1 (MQ=255)
                    ccATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTg                                                                                                                               <  1:1008949/150‑1 (MQ=255)
                        cACTGTCGCTCCAGCGCAAACGCTGACAGACAAAGAATATCAAATGATGCGTAAGGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATc                                                                                                                          <  5:503047/151‑1 (MQ=255)
                        cACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAt                                                                                                                           <  4:1198929/150‑1 (MQ=255)
                        cACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAt                                                                                                                           <  6:344863/150‑1 (MQ=255)
                        cACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATc                                                                                                                          <  8:1361747/151‑1 (MQ=255)
                        cACTGGCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTAt                                                                                                                           <  7:137667/150‑1 (MQ=255)
                          cTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCg                                                                                                                         >  6:813337/1‑150 (MQ=255)
                            gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGaaa                                                                                                                      <  3:879206/151‑1 (MQ=255)
                            gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGaa                                                                                                                       <  5:1471406/150‑1 (MQ=255)
                            gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGaa                                                                                                                       <  1:1400364/150‑1 (MQ=255)
                            gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGaa                                                                                                                       <  2:815076/150‑1 (MQ=255)
                                cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGaa                                                                                                                  <  6:706711/151‑1 (MQ=255)
                                cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGaa                                                                                                                  <  5:821933/151‑1 (MQ=255)
                                cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGa                                                                                                                   >  7:606329/1‑150 (MQ=255)
                                 gcCAGCGCAAACGCGGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAc                                                                                                                 <  3:354202/151‑1 (MQ=255)
                                  ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAcc                                                                                                                >  4:767242/1‑151 (MQ=255)
                                   cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAccc                                                                                                               <  7:1565593/151‑1 (MQ=255)
                                     gCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAc                                                                                                             <  4:563570/151‑1 (MQ=255)
                                     gCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAc                                                                                                             >  6:101738/1‑151 (MQ=255)
                                      cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAcg                                                                                                            <  2:996114/151‑1 (MQ=255)
                                       ccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAcgc                                                                                                           <  6:1168941/151‑1 (MQ=255)
                                       ccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCAcgc                                                                                                           >  6:907020/1‑151 (MQ=255)
                                          aaCTCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCTGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCgtg                                                                                                        <  1:20413/151‑1 (MQ=255)
                                           aCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCgtgt                                                                                                       >  3:467954/1‑151 (MQ=255)
                                            cGCTGTCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCGAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTc                                                                                                      <  1:380990/151‑1 (MQ=255)
                                             gCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTcc                                                                                                     <  2:1400414/151‑1 (MQ=255)
                                              cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTccc                                                                                                    >  1:895029/1‑151 (MQ=255)
                                              cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTccc                                                                                                    >  1:1173444/1‑151 (MQ=255)
                                                 aCCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGtt                                                                                                 <  1:562589/151‑1 (MQ=255)
                                                  ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttc                                                                                                <  4:1363804/151‑1 (MQ=255)
                                                   cGACAAAGAATATGAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttct                                                                                               <  5:194000/151‑1 (MQ=255)
                                                    gACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttctt                                                                                              <  8:1145352/151‑1 (MQ=255)
                                                      cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                            <  6:305908/151‑1 (MQ=255)
                                                      cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCAGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                            <  8:903386/151‑1 (MQ=255)
                                                       aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCgg                                                                                           >  4:359108/1‑151 (MQ=255)
                                                         aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGgcc                                                                                         >  6:854364/1‑150 (MQ=255)
                                                          gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGgcgc                                                                                        <  1:1083528/151‑1 (MQ=255)
                                                           aaTATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                       <  3:240794/151‑1 (MQ=255)
                                                               tCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCg                                                                                   >  8:838914/1‑151 (MQ=255)
                                                                cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCg                                                                                   <  3:1327043/150‑1 (MQ=255)
                                                                cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGt                                                                                  >  7:1134815/1‑151 (MQ=255)
                                                                 aaaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTc                                                                                 <  2:105492/151‑1 (MQ=255)
                                                                  aaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                <  8:431190/151‑1 (MQ=255)
                                                                  aaTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                <  5:6054/151‑1 (MQ=255)
                                                                   aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                >  8:767086/1‑150 (MQ=255)
                                                                    tCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGa                                                                               >  5:928176/1‑150 (MQ=255)
                                                                        gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGc                                                                           <  3:1468469/150‑1 (MQ=255)
                                                                        gCGTAACGCCTCGATCGCGGTCCTGCCTCAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTCCGGTGACTCCGAAAAACGCTCGTCTCATTGTTATCGAAATCAACCCACGCGTGTCCCGTTCTACGTCCCTGGCGTCGCAAGCg                                                                          >  2:1149972/1‑151 (MQ=255)
                                                                         cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCgc                                                                         >  8:305267/1‑150 (MQ=255)
                                                                          gTAACGACTCGATGGAGGTGCTGAGAGAAATCGGCGTTGAAACCGGTGGTTACAACGTTCAGATTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAAAGAACCCACCCGAGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                        >  6:1564222/1‑151 (MQ=255)
                                                                           tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                       >  1:1273510/1‑151 (MQ=255)
                                                                           tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                       >  4:275352/1‑151 (MQ=255)
                                                                             aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                     <  3:195494/151‑1 (MQ=255)
                                                                              cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATTCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                    >  2:1571377/1‑151 (MQ=255)
                                                                               gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                   <  6:520075/151‑1 (MQ=255)
                                                                                   cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTTTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTcc                                                                <  7:105706/150‑1 (MQ=255)
                                                                                   cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                               >  8:719936/1‑151 (MQ=255)
                                                                                   cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                               >  4:513859/1‑151 (MQ=255)
                                                                                   cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                               >  7:873480/1‑151 (MQ=255)
                                                                                       ggCGGTGGTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTTCGGTGAATCCGAAAAACGGTCGTCTGTTTTTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAtt                                                           <  5:1293631/151‑1 (MQ=255)
                                                                                       ggCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAtt                                                           <  8:1647867/151‑1 (MQ=255)
                                                                                       ggCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAtt                                                           >  2:941547/1‑151 (MQ=255)
                                                                                            tgctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGAt                                                            <  3:1526533/145‑1 (MQ=255)
                                                                                             gctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaa                                                      >  4:1290031/1‑150 (MQ=255)
                                                                                              ctgcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaa                                                      >  6:1461949/1‑149 (MQ=255)
                                                                                                 cGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                 <  7:1215595/151‑1 (MQ=255)
                                                                                                  gTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggc                                                <  1:726003/151‑1 (MQ=255)
                                                                                                    taaaTCGGCGTTGAAACCGGTGGTTCCAACGTGCTGTTGGGGGTGAATCCCAAAAACGGGCGTCTGATTGTTATCTAAATCACCCCACGCGTGGCCCGTTCTTCGGCGCGGGGGGCCAAAGCGACCGGTTTCCCGATTGCTAAAGTggcg                                               <  5:655690/149‑1 (MQ=255)
                                                                                                     aaaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAACTGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcggc                                             <  5:415993/151‑1 (MQ=255)
                                                                                                      aaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGgcggcg                                            >  4:1547822/1‑151 (MQ=255)
                                                                                                          ggCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAc                                        >  6:1680547/1‑151 (MQ=255)
                                                                                                           gCGTTTAAACCGGTGGTTCCAACGTGCAGTTTGCGGTGAATCCTAAAAAGGGGCGTCTGATTGTTATCGAAATGAACCCACGCGGGGCCGGTTCTTCGGCGCTGGGGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                       <  8:1491364/151‑1 (MQ=255)
                                                                                                            cGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                      <  8:1028092/151‑1 (MQ=255)
                                                                                                            cGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                      >  3:532446/1‑151 (MQ=255)
                                                                                                            cGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                      >  7:345183/1‑151 (MQ=255)
                                                                                                               tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCg                                   >  4:933142/1‑151 (MQ=255)
                                                                                                                 aaaCCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                 >  4:213745/1‑151 (MQ=255)
                                                                                                                    ccGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                              >  3:1130985/1‑151 (MQ=255)
                                                                                                                            tCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                      >  4:1581361/1‑151 (MQ=255)
                                                                                                                              cAACGTTCAGGTTGCGGTGAATCCGAAAAACGGTCGTCTGATGGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                    <  6:1096026/151‑1 (MQ=255)
                                                                                                                               aaCGTTCAGTTTGCGGTGCATCCGAAAAACGGTCGGCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                   <  7:1345623/151‑1 (MQ=255)
                                                                                                                               aaCGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                   >  5:1056109/1‑151 (MQ=255)
                                                                                                                                aCGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                   >  1:1466026/1‑150 (MQ=255)
                                                                                                                                 cGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACCCCCTcgac                 >  7:451063/1‑151 (MQ=255)
                                                                                                                                  gTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacg                <  3:687616/151‑1 (MQ=255)
                                                                                                                                   ttCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacg                >  4:866227/1‑150 (MQ=255)
                                                                                                                                    tCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacga               >  2:628110/1‑150 (MQ=255)
                                                                                                                                     cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa              <  1:135857/150‑1 (MQ=255)
                                                                                                                                     cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa              <  7:79151/150‑1 (MQ=255)
                                                                                                                                     cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa              <  4:1086803/150‑1 (MQ=255)
                                                                                                                                     cAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc             >  2:374284/1‑151 (MQ=255)
                                                                                                                                         ttGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtga          <  8:1406891/150‑1 (MQ=255)
                                                                                                                                         ttGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtga          <  2:1238993/150‑1 (MQ=255)
                                                                                                                                          tGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgatg        >  2:714289/1‑151 (MQ=255)
                                                                                                                                            cGGTGAATCCCAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTATCCCGATAGCTAAAGAGGCGGCGAAACAGGCCGTCGGTTACACCCTCGACGAACtgatga       >  2:1543766/1‑150 (MQ=255)
                                                                                                                                               tGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACgc   >  2:414578/1‑150 (MQ=255)
                                                                                                                                                gAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa   <  8:520003/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAC  >  NC_000913/31536‑31830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑