Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 469,271 G→A G134E (GGG→GAG)  mdlA → putative multidrug ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913469,2710GA95.1% 1309.0 / 38.0 410G134E (GGG→GAG) mdlAputative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (9/11);  new base A (202/187);  total (212/198)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

Only 100 of 418 total aligned reads displayed.

TCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCA  >  NC_000913/469123‑469418
                                                                                                                                                    |                                                                                                                                                   
tCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGATGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAgagg                                                                                                                                                   >  7:1638132/1‑151 (MQ=255)
  ttATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTg                                                                                                                                                 >  8:1667838/1‑151 (MQ=255)
   tATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGt                                                                                                                                                  <  7:515105/149‑1 (MQ=255)
      cAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTg                                                                                                                                              <  2:314322/150‑1 (MQ=255)
           ggCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTNATCGNACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCtg                                                                                                                                        >  2:1608505/1‑151 (MQ=255)
           ggCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGc                                                                                                                                          <  3:1417284/149‑1 (MQ=255)
             gtGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCtggt                                                                                                                                      <  5:206543/150‑1 (MQ=255)
              tGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATACTGAGTTTTACCTGCGTCATCGCAACGATGACATCATGGCTAGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCtggtg                                                                                                                                     >  3:5891/1‑151 (MQ=255)
               gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCtggtg                                                                                                                                     <  6:1209417/150‑1 (MQ=255)
               gTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCtggt                                                                                                                                      <  3:3476/149‑1 (MQ=255)
                 tGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGCCGGTGGTGGAt                                                                                                                                  >  4:576466/1‑151 (MQ=255)
                  gAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGAtt                                                                                                                                 >  6:1006782/1‑151 (MQ=255)
                  gAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGAt                                                                                                                                  <  4:1617576/150‑1 (MQ=255)
                   aaCTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTc                                                                                                                                >  3:892710/1‑151 (MQ=255)
                      tGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGCCGCTGGTGGATTCAcg                                                                                                                             >  5:1048190/1‑150 (MQ=255)
                       gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGATGCTGGTGGATTCACTg                                                                                                                            >  3:1595036/1‑151 (MQ=255)
                       gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTg                                                                                                                            <  4:633111/151‑1 (MQ=255)
                       gCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACATCATGGCTAGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGACGTGCTGACGCTGGTGGATTCACTg                                                                                                                            >  8:544382/1‑151 (MQ=255)
                         gTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGt                                                                                                                          >  3:73392/1‑151 (MQ=255)
                         gTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGt                                                                                                                          <  7:1611656/151‑1 (MQ=255)
                           gAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGACGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTg                                                                                                                         >  8:286636/1‑150 (MQ=255)
                            aaGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGAt                                                                                                                       <  3:153150/151‑1 (MQ=255)
                             aGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATg                                                                                                                      >  1:996841/1‑151 (MQ=255)
                              gATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATgg                                                                                                                     <  5:1207008/151‑1 (MQ=255)
                               attattACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATggg                                                                                                                    <  3:1066471/151‑1 (MQ=255)
                                ttattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGc                                                                                                                   >  1:1542009/1‑151 (MQ=255)
                                 tattaCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCt                                                                                                                  >  8:1086928/1‑151 (MQ=255)
                                     aCCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTgcgc                                                                                                              >  2:408877/1‑151 (MQ=255)
                                              tAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACAGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGTGCTGCGCTGTGTTGAt                                                                                                     >  4:1257674/1‑151 (MQ=255)
                                               aaGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGAtt                                                                                                    <  4:232576/151‑1 (MQ=255)
                                                  ccGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTatg                                                                                                 >  6:1386060/1‑151 (MQ=255)
                                                     gcagcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGCCGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTatgatg                                                                                              >  7:392592/1‑151 (MQ=255)
                                                      cagcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGt                                                                                             >  4:933548/1‑151 (MQ=255)
                                                       agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTc                                                                                            >  5:600886/1‑151 (MQ=255)
                                                       agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTc                                                                                            <  1:1088394/151‑1 (MQ=255)
                                                       agcaTCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTc                                                                                            >  7:877033/1‑151 (MQ=255)
                                                         caTGCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTa                                                                                          <  2:715196/151‑1 (MQ=255)
                                                            ccTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACg                                                                                        <  8:1476777/150‑1 (MQ=255)
                                                            ccTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGc                                                                                       <  2:1456080/151‑1 (MQ=255)
                                                            ccTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGc                                                                                       <  7:239450/151‑1 (MQ=255)
                                                             cTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGc                                                                                       >  3:1277170/1‑150 (MQ=255)
                                                              tGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCa                                                                                      >  5:1676666/1‑150 (MQ=255)
                                                                aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCGGTGTTGATTATGATGTCTACGCAAAt                                                                                   >  6:727829/1‑151 (MQ=255)
                                                                aGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTATGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGAAGTCTACGCAAAt                                                                                   >  4:492428/1‑151 (MQ=255)
                                                                    ttACCTGCGGCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAg                                                                                <  6:1250786/150‑1 (MQ=255)
                                                                     tttcTGTGTCATCGCAGCGTTGATCTCATGGCTCGTGTGATCTATGTCGTTGATGGCGTCGTGTTTGCCGCCGGAGTTGAGGTGGTGTCGCTGGTGGATTCACTGGTGATGGGCTGCTCTTTGTTGTTTATGATGTCTACGCattttatc                                                                               <  6:1654262/147‑8 (MQ=255)
                                                                      aCCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCt                                                                              <  7:1330564/150‑1 (MQ=255)
                                                                       ccTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTgg                                                                            >  4:1480210/1‑151 (MQ=255)
                                                                        cTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGc                                                                           >  6:320186/1‑151 (MQ=255)
                                                                          gCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGc                                                                           >  2:353131/1‑149 (MQ=255)
                                                                          gCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCa                                                                          <  1:1494293/150‑1 (MQ=255)
                                                                           cGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGt                                                                        >  7:1088412/1‑151 (MQ=255)
                                                                            gTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGtt                                                                       >  8:1051497/1‑151 (MQ=255)
                                                                            gTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGtt                                                                       <  8:835498/151‑1 (MQ=255)
                                                                              cATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTg                                                                      <  2:205045/150‑1 (MQ=255)
                                                                              cATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTg                                                                      >  4:35669/1‑150 (MQ=255)
                                                                              cATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTg                                                                      <  4:1535592/150‑1 (MQ=255)
                                                                               aTCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGAc                                                                    >  7:1177249/1‑151 (MQ=255)
                                                                                  gCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCtt                                                                 <  5:279571/151‑1 (MQ=255)
                                                                                   cACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTa                                                                <  4:1369665/151‑1 (MQ=255)
                                                                                    aCCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTAt                                                               >  5:701176/1‑151 (MQ=255)
                                                                                     ccGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTAtt                                                              <  1:309907/151‑1 (MQ=255)
                                                                                       ggTGACCTCATGGCTCGTGCGACCAATGACGTCGAGCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTAtttt                                                            <  5:904385/151‑1 (MQ=255)
                                                                                        gTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTc                                                           <  5:442627/151‑1 (MQ=255)
                                                                                          gACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGATGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTcc                                                          <  8:1436159/150‑1 (MQ=255)
                                                                                           aCCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCt                                                        <  3:1221315/151‑1 (MQ=255)
                                                                                             cTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGt                                                      >  7:713069/1‑151 (MQ=255)
                                                                                                 tGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAAGTAGCTGGCAGTTGACCTTATTTTCCCTGTTGcc                                                  >  1:579321/1‑151 (MQ=255)
                                                                                                  ggCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCg                                                 <  3:99488/151‑1 (MQ=255)
                                                                                                      cGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGAt                                               <  4:866005/149‑1 (MQ=255)
                                                                                                       gTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGcc                                            >  6:1546744/1‑151 (MQ=255)
                                                                                                         gCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAg                                          <  2:1614634/151‑1 (MQ=255)
                                                                                                         gCGACCAATGACGTCGATCGCGGCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCa                                           <  6:929449/150‑1 (MQ=255)
                                                                                                          cGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGt                                         <  5:1351320/151‑1 (MQ=255)
                                                                                                          cGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGt                                         <  7:1510239/151‑1 (MQ=255)
                                                                                                          cGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGt                                         <  5:778643/151‑1 (MQ=255)
                                                                                                           gACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTg                                        <  1:1342919/151‑1 (MQ=255)
                                                                                                               aaTGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATgg                                    <  1:779511/151‑1 (MQ=255)
                                                                                                                 tGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCg                                  >  8:433791/1‑151 (MQ=255)
                                                                                                                  gACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCg                                  <  2:1134699/150‑1 (MQ=255)
                                                                                                                  gACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCg                                  >  6:1148489/1‑150 (MQ=255)
                                                                                                                  gACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCGGTGTTGATAATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGa                                 <  6:1083687/151‑1 (MQ=255)
                                                                                                                    cGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATc                               <  3:1138761/151‑1 (MQ=255)
                                                                                                                       cGATCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCAt                             <  2:1228248/150‑1 (MQ=255)
                                                                                                                         aTCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGCCGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGAt                          >  4:397865/1‑151 (MQ=255)
                                                                                                                         aTCGCGTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGAt                          >  4:774966/1‑151 (MQ=255)
                                                                                                                            gcgTCGTGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTaa                       <  1:869645/151‑1 (MQ=255)
                                                                                                                             cgtcgtGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTa                        >  2:941737/1‑149 (MQ=255)
                                                                                                                             cgtcgtGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAg                      <  3:206486/151‑1 (MQ=255)
                                                                                                                              gtcgtGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAgc                     <  1:1378893/151‑1 (MQ=255)
                                                                                                                                cgtGTTTGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAgcgc                   >  1:183744/1‑151 (MQ=255)
                                                                                                                                      tGCCGCCGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACgg              <  8:1238312/150‑1 (MQ=255)
                                                                                                                                       gccgccGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCg            <  3:436750/151‑1 (MQ=255)
                                                                                                                                       gccgccGGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCg            <  6:391385/151‑1 (MQ=255)
                                                                                                                                         cgccgGAGAAGAGGTGCTGCCGCTGGTTGATTCACTGGGGATGCGCTGCGCTGTGTTGATTCTGTTGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTCTTGCGGATGCCAGTGATGGCGATCATGATTACGCGCAACGGCGAt          <  6:822281/151‑1 (MQ=255)
                                                                                                                                         cgccgGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGAt          >  2:538982/1‑151 (MQ=255)
                                                                                                                                          gccgGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATg         >  8:75019/1‑151 (MQ=255)
                                                                                                                                           ccgGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGc        >  2:447970/1‑151 (MQ=255)
                                                                                                                                            cgGAGAAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCt       >  4:1182070/1‑151 (MQ=255)
                                                                                                                                              gagaAGAGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCGGCGCGGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCttt     <  5:1460844/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCTTATCAACTGGCTGTTGAACTGCGTGAAGATTATTACCGTCAGCTAAGCCGGCAGCATCCTGAGTTTTACCTGCGTCATCGCACCGGTGACCTCATGGCTCGTGCGACCAATGACGTCGATCGCGTCGTGTTTGCCGCCGGAGAAGGGGTGCTGACGCTGGTGGATTCACTGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCA  >  NC_000913/469123‑469418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑