BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	784,677	G→A	A49A (GCC→GCT)	zitB ←	zinc efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	784,677	0	G	A	97.7%	1343.6 / 11.6	399	A49A (GCC→GCT)	zitB	zinc efflux system
Reads supporting (aligned to +/- strand): ref base G (3/6); new base A (186/203); total (190/209)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

Only 100 of 401 total aligned reads displayed.

CACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATG  >  NC_000913/784527‑784824

cACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCg                                                                                                                                                     <  1:440569/151‑1 (MQ=255)

   gAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGcc                                                                                                                                                  >  3:1248592/1‑151 (MQ=255)

   gAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGcc                                                                                                                                                  >  2:732676/1‑151 (MQ=255)

      ggCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATcagccagc                                                                                                                                               <  7:1118762/151‑1 (MQ=255)

        cTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCa                                                                                                                                              >  7:467639/1‑150 (MQ=255)

          aTGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTTAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCaa                                                                                                                                             <  5:1617844/149‑1 (MQ=255)

             gCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGcaa                                                                                                                                        <  7:1489731/151‑3 (MQ=255)

             gCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCa                                                                                                                                        <  2:1602053/151‑1 (MQ=255)

              cATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCAGGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGcc                                                                                                                                         >  5:554810/1‑149 (MQ=255)

              cATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGcc                                                                                                                                         >  1:1219165/1‑149 (MQ=255)

               aTTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCaga                                                                                                                                      <  5:274965/151‑1 (MQ=255)

               aTTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCaga                                                                                                                                      <  3:1113755/151‑1 (MQ=255)

                 tCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCagag                                                                                                                                     <  8:802495/150‑1 (MQ=255)

                  cacaAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGaa                                                                                                                                   <  3:1537793/151‑1 (MQ=255)

                    caAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAAcc                                                                                                                                 >  8:1341480/1‑151 (MQ=255)

                    caAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAAcc                                                                                                                                 <  2:1204622/151‑1 (MQ=255)

                     aaaCGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCa                                                                                                                                <  2:1029522/151‑1 (MQ=255)

                     aaaCGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCa                                                                                                                                <  7:1355224/151‑1 (MQ=255)

                         gccgcGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATGAGCCAGCCATGCCAGAGCACCagca                                                                                                                             >  4:420797/1‑148 (MQ=255)

                          ccgcGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaaag                                                                                                                           <  3:985972/151‑1 (MQ=255)

                          ccgcGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaa                                                                                                                             >  8:1452500/1‑149 (MQ=255)

                           cgcgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCagaaaga                                                                                                                          <  3:279633/151‑1 (MQ=255)

                            gcgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaaagaa                                                                                                                         <  6:49636/151‑1 (MQ=255)

                            gcgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCagaaaga                                                                                                                          >  2:451984/1‑150 (MQ=255)

                               aGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAac                                                                                                                      >  8:369132/1‑151 (MQ=255)

                               aGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAac                                                                                                                      <  7:821355/151‑1 (MQ=255)

                                 cGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAacc                                                                                                                     >  8:791322/1‑150 (MQ=255)

                                    ggTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAaccacc                                                                                                                  <  6:367358/150‑1 (MQ=255)

                                    ggTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAAccacca                                                                                                                 <  4:956353/151‑1 (MQ=255)

                                     gTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCaa                                                                                                                <  5:1142420/151‑1 (MQ=255)

                                       cAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAAc                                                                                                               <  2:1350042/150‑1 (MQ=255)

                                         gTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGAc                                                                                                            <  2:967778/151‑1 (MQ=255)

                                              aGCCTACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTa                                                                                                       <  1:755439/151‑1 (MQ=255)

                                                 cAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTAcc                                                                                                     >  5:507435/1‑150 (MQ=255)

                                                  aaCCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGCACCAGAAAGAAAACCACCAACGACTTCTAcc                                                                                                     >  1:559735/1‑149 (MQ=255)

                                                   aCCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGc                                                                                                  >  1:253256/1‑151 (MQ=255)

                                                     cGAAAGTGTGGCGAATGGTGGGAGGCCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAACCCACCACCGACTTCTACCAGc                                                                                                  >  3:898562/1‑149 (MQ=255)

                                                      gAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCAt                                                                                                >  8:324436/1‑150 (MQ=255)

                                                      gAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCAt                                                                                                >  6:951285/1‑150 (MQ=255)

                                                      gAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCAt                                                                                                >  5:1485944/1‑150 (MQ=255)

                                                        aaGTGTGGCGAATCGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATAAGACCCGCATCAGCCAGCAATGACACAGAACCAGAAAGAAAACCACCAACGACTTCAACCAGCATaa                                                                                              >  3:1648956/1‑150 (MQ=255)

                                                         aGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCNGGGCGGNAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAc                                                                                            <  2:422620/151‑1 (MQ=255)

                                                         aGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAc                                                                                            <  6:1231976/151‑1 (MQ=255)

                                                         aGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAc                                                                                            >  1:1042535/1‑151 (MQ=255)

                                                          gtgtGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAcc                                                                                           <  6:1537138/151‑1 (MQ=255)

                                                                 gAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTGCCAGCATAAACCCGGCAgg                                                                                    >  2:203662/1‑150 (MQ=255)

                                                                 gAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAgg                                                                                    >  2:1179854/1‑150 (MQ=255)

                                                                    tggtggGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCAc                                                                                 >  7:1596052/1‑151 (MQ=255)

                                                                     ggtggGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACg                                                                                <  3:776038/151‑1 (MQ=255)

                                                                        gggAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCg                                                                             <  4:829599/151‑1 (MQ=255)

                                                                         ggaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGCACCAGAAAGAAACCCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCg                                                                             >  8:772808/1‑150 (MQ=255)

                                                                         ggaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGa                                                                            <  5:1441451/151‑1 (MQ=255)

                                                                          gaggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGaa                                                                           <  3:432090/151‑1 (MQ=255)

                                                                           aggaCGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGaa                                                                           >  7:119869/1‑150 (MQ=255)

                                                                             gacgacGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGc                                                                        <  8:679901/151‑1 (MQ=255)

                                                                              acgacgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGc                                                                        >  4:1585854/1‑150 (MQ=255)

                                                                              acgacgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCa                                                                       <  7:265481/151‑1 (MQ=255)

                                                                               cgacgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCAt                                                                      <  3:1367572/151‑1 (MQ=255)

                                                                                gacgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATa                                                                     <  3:694666/151‑1 (MQ=255)

                                                                                  cgTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATaca                                                                   <  8:595110/151‑1 (MQ=255)

                                                                                   gTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATaca                                                                   >  1:301063/1‑150 (MQ=255)

                                                                                     gAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATacaac                                                                 <  8:1585394/150‑1 (MQ=255)

                                                                                     gAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATacaac                                                                 >  1:1691112/1‑150 (MQ=255)

                                                                                      aaaaTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCACTATCGGTCAACATAAGACCCGCATCAGCCAGCAATGCCAGAGAACCAGACAGACACCCAGCATCGACTTCTACCAGCATCAACCCTGCAGTCACGCCGAAAGCAGacaagcc                                                               >  6:207430/1‑148 (MQ=255)

                                                                                         aTTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGcg                                                            <  4:267674/151‑1 (MQ=255)

                                                                                          ttGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAACCCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGcg                                                            >  6:1133559/1‑150 (MQ=255)

                                                                                             cACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGCTCTACATATGGCCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCGACCAGCATCAACCCGCCAGTCCCGCCGCACGCCTACACCAAGcgacg                                                         >  2:54566/1‑150 (MQ=255)

                                                                                              ccGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAg                                                       <  4:563598/150‑1 (MQ=255)

                                                                                                 gCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCat                                                    <  8:1243427/151‑1 (MQ=255)

                                                                                                   gAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCatta                                                  <  1:1154417/151‑1 (MQ=255)

                                                                                                   gAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCatta                                                  <  6:277655/151‑1 (MQ=255)

                                                                                                    aGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCattat                                                 <  6:1367224/151‑1 (MQ=255)

                                                                                                      tAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCattatt                                                >  5:680367/1‑150 (MQ=255)

                                                                                                       aGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATtattat                                              <  5:1667121/151‑1 (MQ=255)

                                                                                                            gAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTAtcttct                                         >  4:313565/1‑151 (MQ=255)

                                                                                                            gAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTAtcttct                                         <  4:713638/151‑1 (MQ=255)

                                                                                                             aaaaGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTg                                        >  1:1481704/1‑151 (MQ=255)

                                                                                                             aaaaGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTg                                        <  6:1280744/151‑1 (MQ=255)

                                                                                                             aaaaGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTAGTATCTTCTg                                        >  2:306879/1‑151 (MQ=255)

                                                                                                              aaaGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTgg                                       <  2:1433441/151‑1 (MQ=255)

                                                                                                                   aGGGCGGCAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGgt                                  >  4:526073/1‑151 (MQ=255)

                                                                                                                      gcggcAGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGtgtg                               <  3:594486/151‑1 (MQ=255)

                                                                                                                      gcggcAGTATCGGTCAAAATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGtgtg                               <  7:67945/151‑1 (MQ=255)

                                                                                                                        gggaGTATCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGtgtg                               >  7:303155/4‑149 (MQ=255)

                                                                                                                              aTCGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACgt                        <  6:1647808/150‑1 (MQ=255)

                                                                                                                                cGGTCAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACgtgt                      <  3:615282/150‑1 (MQ=255)

                                                                                                                                    cAACATATGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTgtgtga                  <  4:1278519/150‑1 (MQ=255)

                                                                                                                                         tatGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTgtgtgagtgtg             >  1:156524/1‑150 (MQ=255)

                                                                                                                                          atGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTgtgtgagtgtg             >  8:50672/1‑149 (MQ=255)

                                                                                                                                           tGACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTgtgagtgtgagt          >  5:1329832/1‑151 (MQ=255)

                                                                                                                                            gACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTgtgagtgtgagt          <  4:1508003/150‑1 (MQ=255)

                                                                                                                                            gACCCGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTgtgagtgtgagt          <  8:53950/150‑1 (MQ=255)

                                                                                                                                              cccGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTgcg       <  8:1022325/151‑1 (MQ=255)

                                                                                                                                              cccGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTgcg       >  3:359828/1‑151 (MQ=255)

                                                                                                                                                cGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGcc     <  7:535785/151‑1 (MQ=255)

                                                                                                                                                cGCATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGcc     >  3:105888/1‑151 (MQ=255)

                                                                                                                                                  cATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCAt   >  1:926745/1‑151 (MQ=255)

                                                                                                                                                  cATCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCAt   >  5:802821/1‑151 (MQ=255)

                                                                                                                                                   aTCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATg  <  4:1171961/151‑1 (MQ=255)

                                                                                                                                                   aTCAGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATg  <  6:700510/151‑1 (MQ=255)

CACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCCGGCAGTCACGCCGAAAGCATACAACAAGCGACGAGCATTATTATCTTCTGGCAGGTGTGAGGACGTGTGTGAGTGTGAGTGCGCCATG  >  NC_000913/784527‑784824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`