Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,297,496 G→A A209V (GCT→GTT)  adhE ← fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,297,4960GA97.0% 1340.9 / 18.6 401A209V (GCT→GTT) adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base A (195/192);  total (200/201)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 401 total aligned reads displayed.

AAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGG  >  NC_000913/1297347‑1297643
                                                                                                                                                     |                                                                                                                                                   
aaaCAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGc                                                                                                                                                    <  8:496036/151‑1 (MQ=255)
    agagTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGt                                                                                                                                                <  3:1412841/151‑1 (MQ=255)
       gTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTa                                                                                                                                             <  3:1294867/151‑1 (MQ=255)
         aaacaacaacaaaAGACTGTTCAGAATCACAGATTACGCCGTTGTCGAAGGTTTTTGACATCATTACAGATGCAACTTCACTTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCTCCTACACCGATAACTGGTTTaa                                                                                                                                            <  3:109923/149‑2 (MQ=255)
          aacaacaacaacAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCg                                                                                                                                          >  5:851760/1‑151 (MQ=255)
              acaacaacaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTCCAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGc                                                                                                                                      <  3:1192379/151‑1 (MQ=255)
               caacaacaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCt                                                                                                                                     >  1:232516/1‑151 (MQ=255)
                 acaacaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGt                                                                                                                                   >  7:1064139/1‑151 (MQ=255)
                 acaacaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGt                                                                                                                                   >  3:1490464/1‑151 (MQ=255)
                    acaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATg                                                                                                                                >  2:482637/1‑151 (MQ=255)
                    acaGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATg                                                                                                                                >  7:1571180/1‑151 (MQ=255)
                     caGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCTGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGc                                                                                                                               >  6:1561766/1‑151 (MQ=255)
                      aGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCg                                                                                                                              >  2:643042/1‑151 (MQ=255)
                      aGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCg                                                                                                                              <  1:724014/151‑1 (MQ=255)
                          tGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCtt                                                                                                                          >  5:416899/1‑151 (MQ=255)
                           gTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCt                                                                                                                           <  5:1275857/149‑1 (MQ=255)
                            ttCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCttt                                                                                                                         >  8:75239/1‑150 (MQ=255)
                             tCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATTCGGCTTTa                                                                                                                        >  1:467858/1‑150 (MQ=255)
                                gAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCa                                                                                                                    <  8:1459355/151‑1 (MQ=255)
                                gAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCa                                                                                                                    <  1:1201412/151‑1 (MQ=255)
                                 aaGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCAt                                                                                                                   <  2:149370/151‑1 (MQ=255)
                                 aaGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCAt                                                                                                                   <  1:1332911/151‑1 (MQ=255)
                                    cacaGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGcc                                                                                                                >  1:1276510/1‑151 (MQ=255)
                                      caGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCg                                                                                                              <  5:30048/151‑1 (MQ=255)
                                       aGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCgg                                                                                                             >  5:724685/1‑151 (MQ=255)
                                        taTTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCggc                                                                                                            >  1:887820/2‑150 (MQ=255)
                                        gATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCggc                                                                                                            >  4:676522/1‑150 (MQ=255)
                                        gATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGa                                                                                                            <  2:1280491/151‑1 (MQ=255)
                                         aTTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGac                                                                                                           >  7:1263862/1‑151 (MQ=255)
                                         aTTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGac                                                                                                           >  3:742245/1‑151 (MQ=255)
                                            aCGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGacca                                                                                                         <  4:1241292/150‑1 (MQ=255)
                                             cGCCGTTGTCGAAGGTTTTGGACATCAGTAAAGATGCAACTGCACGTTTGATATCACCAGTTTCATCGAAAACAACAGGAGTCTTGCCCGCGCCTACACCGATAACTGCTTTACCGGAGCTGAATGTGGCTTTAACCATCCCCGGaccacc                                                                                                       >  7:1496473/1‑151 (MQ=255)
                                              gCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACAATTCCAGGcccacc                                                                                                       >  7:818699/1‑150 (MQ=255)
                                                cGTTGTCGAAGGTTTTGGACATGAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATTACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGt                                                                                                    <  3:1641748/151‑1 (MQ=255)
                                                 gTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTc                                                                                                   >  7:560831/1‑151 (MQ=255)
                                                 gTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTc                                                                                                   >  4:999153/1‑151 (MQ=255)
                                                 gTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTc                                                                                                   <  6:1233946/151‑1 (MQ=255)
                                                   tGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGGACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcg                                                                                                  <  3:509344/150‑1 (MQ=255)
                                                   tGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgc                                                                                                 >  4:78621/1‑151 (MQ=255)
                                                   tGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcg                                                                                                  <  5:1458565/150‑1 (MQ=255)
                                                    gTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgc                                                                                                 <  2:33563/150‑1 (MQ=255)
                                                     tCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgcg                                                                                                >  5:494220/1‑150 (MQ=255)
                                                     tCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTcgcg                                                                                                >  7:702393/1‑150 (MQ=255)
                                                      cGAAGGTTTTCGACATCAGTATAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAg                                                                                              <  6:813332/151‑1 (MQ=255)
                                                       gAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGatg                                                                                             >  5:1157972/1‑149 (MQ=255)
                                                       gAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAgg                                                                                             >  7:1350021/1‑151 (MQ=255)
                                                        aaGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGa                                                                                            <  6:1476954/151‑1 (MQ=255)
                                                         aGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCAGGACCACCAGTCGCGAGGAt                                                                                           <  6:1136145/151‑1 (MQ=255)
                                                           gTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCa                                                                                         <  8:428812/151‑1 (MQ=255)
                                                            ttttGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATc                                                                                          >  5:1681233/1‑149 (MQ=255)
                                                              ttGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGt                                                                                      >  6:133149/1‑151 (MQ=255)
                                                                  aCATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAt                                                                                  <  8:301108/151‑1 (MQ=255)
                                                                  aCATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGAt                                                                                  >  3:362753/1‑151 (MQ=255)
                                                                   cATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTCTATGCCGCTTTAACCATGCCCGGACCACCAGACGCGAGGATCacctacat                                                                                  >  6:210325/1‑143 (MQ=255)
                                                                      cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCt                                                                              >  1:1407913/1‑151 (MQ=255)
                                                                      cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCt                                                                              >  1:412984/1‑151 (MQ=255)
                                                                      cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCt                                                                              <  1:760018/151‑1 (MQ=255)
                                                                      cAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCt                                                                              >  7:1641247/1‑151 (MQ=255)
                                                                       aGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTg                                                                             >  2:1052622/1‑151 (MQ=255)
                                                                        gTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTgg                                                                            >  3:585961/1‑151 (MQ=255)
                                                                          aCAGATGCAACTCCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGTTTTACCGGAGCTGTATGCGGCTTTAACCATTCCCGGACCACCAGTCGCGAGGATCATGTTGATGTCTgttt                                                                          <  1:1608043/151‑4 (MQ=255)
                                                                             gATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGggtggt                                                                       <  6:956092/151‑1 (MQ=255)
                                                                              aTGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGgtggtg                                                                      <  8:191526/151‑1 (MQ=255)
                                                                                  aaCTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATc                                                                  >  3:832772/1‑151 (MQ=255)
                                                                                         cGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCACGTTGCTGTCTGGGTGGTGCATCAGTGCGt                                                           >  4:1663361/1‑151 (MQ=255)
                                                                                         cGTTTGATATCAGCAGTTTCATCGATAACAACAGGACTGTTCCCCGCGCCTACACCGATAACTGGTTAACCGGAGCTGTATGCGGCTTAAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCGTGGTGGTGCATCACTGCgg                                                           >  2:199688/1‑150 (MQ=255)
                                                                                          gTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGtt                                                          <  4:392329/151‑1 (MQ=255)
                                                                                             tGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCAGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGACGATCAGGTTGATGTCTGGGTGGTGCATAAGTGCGtt                                                          >  8:678503/1‑148 (MQ=255)
                                                                                             tGATATCAGCAGTTTCATCGATAACAACAGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGCCCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGtt                                                          >  5:1605017/1‑148 (MQ=255)
                                                                                                   cagcagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTc                                                 >  6:418697/1‑151 (MQ=255)
                                                                                                     gcagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCaa                                               <  8:1618418/151‑1 (MQ=255)
                                                                                                     gcagTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCaa                                               <  4:1130097/151‑1 (MQ=255)
                                                                                                          ttCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGa                                           >  5:1339790/1‑150 (MQ=255)
                                                                                                           tCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGaa                                          <  7:416126/150‑1 (MQ=255)
                                                                                                            aaTCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAgg                                        <  3:282105/150‑1 (MQ=255)
                                                                                                             aTCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGt                                       <  1:983071/151‑1 (MQ=255)
                                                                                                               cGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGtt                                      <  2:853255/150‑1 (MQ=255)
                                                                                                               cGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGCTGCATCAGTGCGGTAGACAGTTCAACAGAAGGTTg                                     >  4:1498699/1‑151 (MQ=255)
                                                                                                                gATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTg                                     >  5:749774/1‑150 (MQ=255)
                                                                                                                 aTAACAACTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgat                                   <  1:830825/151‑1 (MQ=255)
                                                                                                                    acaacTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcga                                <  8:116256/151‑1 (MQ=255)
                                                                                                                      aacTGGAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcgatc                              <  4:509487/151‑1 (MQ=255)
                                                                                                                      aacTGGAGTGTGGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTgatcgatc                              <  1:1014550/151‑1 (MQ=255)
                                                                                                                          ggAGTGTTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCa                            <  2:749553/149‑1 (MQ=255)
                                                                                                                             gtgtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTGTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGCTCCacgcgc                       >  1:657104/1‑146 (MQ=255)
                                                                                                                             gtgtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGa                       <  6:538559/151‑1 (MQ=255)
                                                                                                                               gtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGAt                      <  3:955863/150‑1 (MQ=255)
                                                                                                                               gtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                     <  4:1356229/151‑1 (MQ=255)
                                                                                                                               gtTGCCCGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATc                     <  8:350533/151‑1 (MQ=255)
                                                                                                                                   cccGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAt                 <  6:302922/151‑1 (MQ=255)
                                                                                                                                    ccGCGCCTACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGAt                 >  1:1488383/1‑150 (MQ=255)
                                                                                                                                           tACACCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCCCCAGTCGCGACGATCACGTTGATCTCAGCGTGGTGCATCAGTGCCTTACACACTTCAACACAAGCATGATCGATCCAGCCGATCACATcaatcgc          >  4:578745/1‑144 (MQ=255)
                                                                                                                                            acacCGATAACTGGTTTACCGGAGCTGTATTCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCgg          <  7:886616/149‑1 (MQ=255)
                                                                                                                                            acacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGa         <  7:936513/150‑1 (MQ=255)
                                                                                                                                            acacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAg        <  1:331752/151‑1 (MQ=255)
                                                                                                                                             cacCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGACGATCAGCTTGATGTCAGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAACGGTGATCGAACCACCCGATCAGATCTTTCGGAGc       >  2:535311/1‑151 (MQ=255)
                                                                                                                                              acCGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGc       >  2:952146/1‑150 (MQ=255)
                                                                                                                                                cGATAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCAcc    >  2:1345317/1‑151 (MQ=255)
                                                                                                                                                 gATAGCTTGTTTACCGGATCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGGTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCg   <  7:316619/151‑1 (MQ=255)
                                                                                                                                                  aTAACTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCgg  <  5:63151/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTTTAACCATGCCCGGACCACCAGTCGCGAGGATCAGGTTGATGTCTGGGTGGTGCATCAGTGCGTTAGACAGTTCAACAGAAGGTTGATCGATCCAGCCGATCAGATCTTTCGGAGCACCGG  >  NC_000913/1297347‑1297643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: