Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 76,249 G→A Q351* (CAA→TAA)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091376,2490GA95.6% 1345.8 / 31.5 411Q351* (CAA→TAA) sgrRtranscriptional DNA‑binding transcriptional activator of sgrS sRNA
Reads supporting (aligned to +/- strand):  ref base G (8/10);  new base A (179/213);  total (187/224)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 411 total aligned reads displayed.

CCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCC  >  NC_000913/76101‑76395
                                                                                                                                                    |                                                                                                                                                  
ccAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACt                                                                                                                                                  <  1:1435951/151‑1 (MQ=255)
   gCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGAt                                                                                                                                               <  8:1500241/151‑1 (MQ=255)
   gCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGAt                                                                                                                                               >  2:1227102/1‑151 (MQ=255)
    ccGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGAtt                                                                                                                                              <  5:661720/151‑1 (MQ=255)
     cGGTTTTTCGCTCTTTATGGTGCGGGCATGGGGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTc                                                                                                                                             <  5:1591734/151‑1 (MQ=255)
      ggTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCg                                                                                                                                            <  7:1589254/151‑1 (MQ=255)
       gTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGc                                                                                                                                           >  6:25609/1‑151 (MQ=255)
       gTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGCGGGACCAGTCCATAAGCCCGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTACTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTCATTCGc                                                                                                                                           >  1:1203280/1‑151 (MQ=255)
         ttttCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGccc                                                                                                                                         >  5:368670/1‑151 (MQ=255)
             cGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCcgcg                                                                                                                                     <  3:549054/151‑1 (MQ=255)
             cGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCcgc                                                                                                                                      <  7:729901/150‑1 (MQ=255)
                 ctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATgg                                                                                                                                 >  6:1332971/1‑151 (MQ=255)
                  tttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATggg                                                                                                                                >  5:1236432/1‑151 (MQ=255)
                  tttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTAATGATTAGCCCCGCGATggg                                                                                                                                >  8:1591227/1‑151 (MQ=255)
                    tATGGTGCGGGCAGGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGGTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGt                                                                                                                               <  2:1526563/150‑1 (MQ=255)
                     aTGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGACCCGCGATGGGTGc                                                                                                                             >  8:1053810/1‑151 (MQ=255)
                      tGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCg                                                                                                                            >  1:394806/1‑151 (MQ=255)
                       ggTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCgg                                                                                                                           <  7:613998/151‑1 (MQ=255)
                       ggTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCgg                                                                                                                           >  4:616669/1‑151 (MQ=255)
                         tGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCt                                                                                                                         >  7:1160381/1‑151 (MQ=255)
                          gCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCt                                                                                                                         <  6:1019979/150‑1 (MQ=255)
                            gggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTc                                                                                                                      <  5:1339730/151‑1 (MQ=255)
                            gggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTc                                                                                                                      >  1:1020202/1‑151 (MQ=255)
                             ggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTCCGGCTGTCg                                                                                                                     <  8:1149244/151‑1 (MQ=255)
                              gCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCg                                                                                                                     >  6:9630/1‑150 (MQ=255)
                                aTGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAAc                                                                                                                  <  3:1369162/151‑1 (MQ=255)
                                   gTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGt                                                                                                               <  5:1407625/151‑1 (MQ=255)
                                      ccAACGGGGGAGCAGTCCATAAGCCGGGAACCACCGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTCCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTaaa                                                                                                            <  7:172631/151‑1 (MQ=255)
                                      ccAACGGGGGAGCAGTCCATAAGCCGCGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTCGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTaaa                                                                                                            <  2:41198/151‑1 (MQ=255)
                                       cAACGGGGGAGCATTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCACTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAt                                                                                                           <  8:274195/151‑1 (MQ=255)
                                       cAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAt                                                                                                           <  3:1221171/151‑1 (MQ=255)
                                        aaCGGGGGAGCAGTCCATAAGCCGTGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAta                                                                                                          <  3:375416/151‑1 (MQ=255)
                                         aCGGGGGAGCAGTCGATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCATCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACGGAGTGCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAtag                                                                                                         <  6:716208/151‑1 (MQ=255)
                                         aCGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAtag                                                                                                         <  4:40776/151‑1 (MQ=255)
                                           gggggAGCAGTGCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAtagta                                                                                                       <  8:1108529/151‑1 (MQ=255)
                                           gggggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAAtagta                                                                                                       <  5:258953/151‑1 (MQ=255)
                                             gggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGc                                                                                                     <  8:423999/151‑1 (MQ=255)
                                             gggAGCAGGCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGc                                                                                                     <  3:1500954/151‑1 (MQ=255)
                                               gAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCa                                                                                                    >  7:100069/1‑150 (MQ=255)
                                               gAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCa                                                                                                    >  8:853913/1‑150 (MQ=255)
                                                   aGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCtt                                                                                               <  1:269802/151‑1 (MQ=255)
                                                   aGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCtt                                                                                               <  6:1663067/151‑1 (MQ=255)
                                                        aTAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAAGAGTAAATAGTAGCACCCTtcctcc                                                                                          >  2:312100/1‑151 (MQ=255)
                                                        aTAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTtcctcc                                                                                          >  3:1622573/1‑151 (MQ=255)
                                                          aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCa                                                                                         <  5:848729/150‑1 (MQ=255)
                                                          aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCa                                                                                         <  2:770567/150‑1 (MQ=255)
                                                          aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAg                                                                                        <  1:704793/151‑1 (MQ=255)
                                                          aaGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAg                                                                                        <  2:823783/151‑1 (MQ=255)
                                                             ccGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAggcg                                                                                     <  3:792130/151‑1 (MQ=255)
                                                              cGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAggcgg                                                                                    <  4:887548/151‑1 (MQ=255)
                                                                   aCCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTc                                                                               >  8:1666808/1‑151 (MQ=255)
                                                                     cacaGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCaa                                                                             <  2:26074/151‑1 (MQ=255)
                                                                      acaGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAt                                                                            <  5:414669/151‑1 (MQ=255)
                                                                          ttGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATctct                                                                        >  8:137951/1‑151 (MQ=255)
                                                                             cTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt                                                                     >  2:708178/1‑151 (MQ=255)
                                                                             cTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt                                                                     >  7:498920/1‑151 (MQ=255)
                                                                             cTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCgttt                                                                     >  4:1452831/1‑151 (MQ=255)
                                                                               ggTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCttttt                                                                    <  4:1136515/150‑1 (MQ=255)
                                                                               gctACTGCTCCTCAGCGAAAAAGACCAGATTAGTTGGAGAAAGCACCTACCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCtttt                                                                     <  4:1219684/147‑1 (MQ=255)
                                                                                  aCTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTc                                                                >  4:710393/1‑151 (MQ=255)
                                                                                  aCTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCt                                                                 <  7:34912/150‑1 (MQ=255)
                                                                                  aCTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCt                                                                 <  5:1306268/150‑1 (MQ=255)
                                                                                      ttCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGccc                                                            >  7:1556004/1‑151 (MQ=255)
                                                                                      ttCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGccc                                                            >  1:175929/1‑151 (MQ=255)
                                                                                           cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCtgt                                                         <  7:1382955/149‑1 (MQ=255)
                                                                                           cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTgg                                                       >  5:1080591/1‑151 (MQ=255)
                                                                                           cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTgg                                                       <  1:1550416/151‑1 (MQ=255)
                                                                                           cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTgg                                                       <  6:420142/151‑1 (MQ=255)
                                                                                               gAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCt                                                   >  3:248795/1‑151 (MQ=255)
                                                                                                aaaTAGACCAGATTAGTTGGCGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCtt                                                  <  6:301337/151‑1 (MQ=255)
                                                                                                 aaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCttt                                                 <  1:1238883/151‑1 (MQ=255)
                                                                                                        cAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATc                                          >  4:1581568/1‑151 (MQ=255)
                                                                                                         aGATTAGTTGGAGAAAGCACATAGCTTACCCAGGCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTGCTCGCCCTGTGGACCTTTTAGCATCa                                         <  4:975628/151‑1 (MQ=255)
                                                                                                           aTTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGc                                       <  1:931738/151‑1 (MQ=255)
                                                                                                             tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGccc                                     >  5:559572/1‑151 (MQ=255)
                                                                                                              aGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCt                                    <  3:106742/151‑1 (MQ=255)
                                                                                                                ttGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTcc                                  >  4:491709/1‑151 (MQ=255)
                                                                                                                ttGGAGAAAGCACATAGCTTACCCAGTCCCCGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCATCGCCATGTGGACCTTTTAGCATCAGCACTcc                                  >  3:203177/1‑151 (MQ=255)
                                                                                                                  ggAGAAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCgg                                <  3:734582/151‑1 (MQ=255)
                                                                                                                  ggAGAAAGCACATAGCTTACACAGTCCCTGACTTGCTGATTAGACCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTATCAACCTTCCTCAAGGAGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCgg                                >  1:485486/1‑151 (MQ=255)
                                                                                                                   gagaAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggc                               >  6:526770/1‑151 (MQ=255)
                                                                                                                    agaAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGCACAGTAAATAGTAGCAAGCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggct                              >  6:833919/1‑151 (MQ=255)
                                                                                                                    agaAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggct                              <  8:1219839/151‑1 (MQ=255)
                                                                                                                    agaAACCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCGCGCGATGGGTGCTTCTTTCGAACAGTAAATATTAGAAACCTTCCTCCAGTCGTCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggct                              <  2:1428665/151‑1 (MQ=255)
                                                                                                                     gaAAGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggctg                             <  8:657388/151‑1 (MQ=255)
                                                                                                                      aaaGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggctgg                            >  7:897535/1‑151 (MQ=255)
                                                                                                                        aGCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCggctggct                          >  3:675268/1‑151 (MQ=255)
                                                                                                                         gCACATAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCtc                         <  2:498531/151‑1 (MQ=255)
                                                                                                                          cacaTAGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTGCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCtcg                        >  4:1354958/1‑151 (MQ=255)
                                                                                                                               aGCTTACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCgg                    <  6:1594247/150‑1 (MQ=255)
                                                                                                                                   tACCCAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAAtt               >  1:1136440/1‑151 (MQ=255)
                                                                                                                                      ccAGTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTcc            <  6:1226558/151‑1 (MQ=255)
                                                                                                                                         gTCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGc         <  1:329608/151‑1 (MQ=255)
                                                                                                                                          tCCCTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGc         >  3:997528/1‑150 (MQ=255)
                                                                                                                                           cccTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGc         >  7:1033947/1‑149 (MQ=255)
                                                                                                                                           cccTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGc         >  6:637527/1‑149 (MQ=255)
                                                                                                                                           cccTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCa        <  8:534102/150‑1 (MQ=255)
                                                                                                                                            ccTGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGa      <  7:475424/151‑1 (MQ=255)
                                                                                                                                              tGACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAAc    >  1:643464/1‑151 (MQ=255)
                                                                                                                                               gACTTACTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAAcc   >  3:465043/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCC  >  NC_000913/76101‑76395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: