BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	96.1%	1175.5 / 18.2	333	Y110C (TAT→TGT)	puuP	putrescine importer
Reads supporting (aligned to +/- strand): ref base T (8/5); new base C (181/136); total (191/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 333 total aligned reads displayed.

AAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCA  >  NC_000913/1358712‑1359006

   ttAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATcc                                                                                                                                                >  8:1409048/1‑150 (MQ=255)

    tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATcc                                                                                                                                                >  5:1102803/1‑149 (MQ=255)

    tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCca                                                                                                                                               <  7:1459131/150‑1 (MQ=255)

     aGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagc                                                                                                                                             >  6:1316781/1‑151 (MQ=255)

      gCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagca                                                                                                                                            <  3:468158/151‑1 (MQ=255)

       cGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagc                                                                                                                                             >  8:917062/1‑149 (MQ=255)

       cGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagc                                                                                                                                             >  6:682233/1‑149 (MQ=255)

        gACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCagcagc                                                                                                                                          <  8:1354602/151‑1 (MQ=255)

        gACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCagcagc                                                                                                                                          >  3:1568087/1‑151 (MQ=255)

          ccAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCg                                                                                                                                         >  7:280590/1‑150 (MQ=255)

                   cGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGcc                                                                                                                               >  4:1069512/1‑151 (MQ=255)

                          cAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATa                                                                                                                        <  1:1614463/151‑1 (MQ=255)

                               tCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATc                                                                                                                    <  6:135920/150‑1 (MQ=255)

                                cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCg                                                                                                                  >  7:1599576/1‑151 (MQ=255)

                                  cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGGGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGAc                                                                                                                <  1:1393836/151‑1 (MQ=255)

                                  cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGAc                                                                                                                >  2:487946/1‑151 (MQ=255)

                                  cggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGAc                                                                                                                >  6:536662/1‑151 (MQ=255)

                                   ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACg                                                                                                               <  6:371309/151‑1 (MQ=255)

                                    gcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGt                                                                                                              >  1:1320758/1‑151 (MQ=255)

                                    gcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGt                                                                                                              >  1:842314/1‑151 (MQ=255)

                                     gggTTAAAATGGCGACGAATGTTACCACCCACACCCACGGCGGCAGTTCCGGGAAGAGGGCGGAGAGATATATTTTCGCCAACAAGACGTTAATCATCTGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg                                                                                                             <  6:1220060/150‑1 (MQ=255)

                                       gTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCg                                                                                                           <  1:1149213/151‑1 (MQ=255)

                                          aaaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCggg                                                                                                         <  5:684107/150‑1 (MQ=255)

                                          aaaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGt                                                                                                        <  1:743034/151‑1 (MQ=255)

                                          aaaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGt                                                                                                        <  7:1146633/151‑1 (MQ=255)

                                           aaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGtt                                                                                                       >  5:83764/1‑151 (MQ=255)

                                            aaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGtt                                                                                                       >  4:1530399/1‑150 (MQ=255)

                                             aTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCAGACGTGCGGGTTa                                                                                                      >  7:939150/1‑150 (MQ=255)

                                              aggCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAAt                                                                                                    <  8:756289/150‑1 (MQ=255)

                                               ggCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATc                                                                                                   <  3:585151/151‑1 (MQ=255)

                                               ggCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATc                                                                                                   <  5:927161/151‑1 (MQ=255)

                                                gCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCg                                                                                                  <  5:193808/151‑1 (MQ=255)

                                                  gacgaAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTTCGGGTTAATAGAc                                                                                                >  6:421273/1‑151 (MQ=255)

                                                  gacgaAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGAc                                                                                                >  3:846587/1‑151 (MQ=255)

                                                    cgaAGGTTACCACCCACACCCATGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtt                                                                                              <  4:363847/151‑1 (MQ=255)

                                                    cgaAGGTTACCACCCACACCCACGGCGGCACTTCCTTTAAGAGGTCGGAGAGATAGATTTTCGACAACAAGACGTTAATAATCGTCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACAATAAATCAGACGTGCGGGTTAATCGACtt                                                                                              >  1:1048256/1‑151 (MQ=255)

                                                       aGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTg                                                                                           >  2:404171/1‑151 (MQ=255)

                                                        ggTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGGGGAGAGATAGATTATCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATCACCAGCCGAGCATAAATCCGACGTGCGGGTTAATGGACTTTTgc                                                                                          >  1:1120139/1‑151 (MQ=255)

                                                         gTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgcg                                                                                         >  4:438866/1‑151 (MQ=255)

                                                           tACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTgcgc                                                                                        <  5:605432/150‑1 (MQ=255)

                                                            accaccCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGt                                                                                      <  8:263279/151‑1 (MQ=255)

                                                             ccacccacACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTa                                                                                     <  7:1357892/151‑1 (MQ=255)

                                                                 ccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGta                                                                                 <  1:333584/151‑1 (MQ=255)

                                                                 ccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGt                                                                                  >  2:791772/1‑150 (MQ=255)

                                                                  cacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                >  2:1681065/1‑151 (MQ=255)

                                                                   acacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                >  3:968876/1‑150 (MQ=255)

                                                                    cacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                >  7:1307566/1‑149 (MQ=255)

                                                                    cacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                >  4:808642/1‑149 (MQ=255)

                                                                    cacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGtat                                                                                >  7:745492/1‑149 (MQ=255)

                                                                    cacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAg                                                                              <  7:350039/151‑1 (MQ=255)

                                                                    cacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGAACGtat                                                                                >  4:1385693/1‑149 (MQ=255)

                                                                       ccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGcc                                                                           <  5:1432687/151‑1 (MQ=255)

                                                                        cacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCg                                                                          >  1:580581/1‑151 (MQ=255)

                                                                        cacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCg                                                                          >  5:1507531/1‑151 (MQ=255)

                                                                          cggcggCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGa                                                                         >  7:481253/1‑150 (MQ=255)

                                                                           ggcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAc                                                                       <  8:1575223/151‑1 (MQ=255)

                                                                            gcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc                                                                      <  2:70628/151‑1 (MQ=255)

                                                                            gcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACCTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc                                                                      <  8:326905/151‑1 (MQ=255)

                                                                             cggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCg                                                                     >  6:48952/1‑151 (MQ=255)

                                                                                cACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGcc                                                                  >  8:557301/1‑151 (MQ=255)

                                                                                 aCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCt                                                                 >  8:457507/1‑151 (MQ=255)

                                                                                   ttCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTTCGCGTAGGTATAGGCCGAACCGGCCTcc                                                               >  6:963198/1‑151 (MQ=255)

                                                                                   ttCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTcc                                                               >  8:1490194/1‑151 (MQ=255)

                                                                                    tCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTc                                                                <  5:32691/149‑1 (MQ=255)

                                                                                    tCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGCGTTAATCGACTTTTCCGCGTAGGTATAGGCCGAACCGGCCTCCg                                                              >  5:747336/1‑151 (MQ=255)

                                                                                         gAAGAGGGCGGATAGATAGAATTTCGCCAACAAGACGTTAATCATCCGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACCTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAAc                                                         <  6:1275888/151‑1 (MQ=255)

                                                                                             agGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGc                                                     >  4:989073/1‑151 (MQ=255)

                                                                                             agGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTACGTATAGGCCGAACCGGCCTCCGGAAACTGGc                                                     >  6:309008/1‑151 (MQ=255)

                                                                                                 cGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAAc                                                 <  3:448948/151‑1 (MQ=255)

                                                                                                  ggAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAAcc                                                >  7:512170/1‑151 (MQ=255)

                                                                                                          agatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGcc                                        <  1:513784/151‑1 (MQ=255)

                                                                                                           gatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGCACCAGTTTGCCg                                       >  6:1555402/1‑151 (MQ=255)

                                                                                                            atTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGATCCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGt                                      >  2:20157/1‑151 (MQ=255)

                                                                                                             ttttCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGt                                      >  4:260664/1‑150 (MQ=255)

                                                                                                               ttCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGc                                   >  3:778809/1‑151 (MQ=255)

                                                                                                                 cGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGCACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTg                                 >  4:567032/1‑151 (MQ=255)

                                                                                                                  gCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTg                                 >  3:1385087/1‑150 (MQ=255)

                                                                                                                   ccAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCTGGCGAACCAGTTTGCCGTAGCTGAt                               >  1:394993/1‑151 (MQ=255)

                                                                                                                    caacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGAt                               >  1:667738/1‑150 (MQ=255)

                                                                                                                    caacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGAt                               >  4:1163536/1‑150 (MQ=255)

                                                                                                                    caacaaGACGTTAATCATCGACAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGCGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGAt                               >  7:962109/1‑150 (MQ=255)

                                                                                                                        aaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCgg                          >  6:1059787/1‑151 (MQ=255)

                                                                                                                        aaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCgg                          >  8:1057385/1‑151 (MQ=255)

                                                                                                                        aaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTACGTATAGGCCGAAACGGCCTCCGGAAACTGGCGAACCAGTTTGGCGTAGCTGATAGCgg                          >  6:589244/1‑151 (MQ=255)

                                                                                                                           aCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGtc                        >  3:1212837/1‑149 (MQ=255)

                                                                                                                            cGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTaaa                      <  2:1577441/151‑1 (MQ=255)

                                                                                                                               tAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAAc                     >  5:1255544/1‑149 (MQ=255)

                                                                                                                               tAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACa                    <  7:843199/150‑1 (MQ=255)

                                                                                                                                aaTCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGc                  >  4:75251/1‑151 (MQ=255)

                                                                                                                                 atcatcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGc                  >  8:1127639/1‑150 (MQ=255)

                                                                                                                                    atcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGCACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCccac              >  2:1268552/1‑151 (MQ=255)

                                                                                                                                    atcGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGCACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac              >  1:1540274/1‑151 (MQ=255)

                                                                                                                                          aaaaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc         >  5:1357752/1‑150 (MQ=255)

                                                                                                                                           aaaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccag       <  5:112517/151‑1 (MQ=255)

                                                                                                                                           aaaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc         >  3:180498/1‑149 (MQ=255)

                                                                                                                                           aaaaCAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccag       <  1:1101547/151‑1 (MQ=255)

                                                                                                                                            aaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagc      <  1:1263068/151‑1 (MQ=255)

                                                                                                                                             aaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcg     <  5:498953/151‑1 (MQ=255)

                                                                                                                                              agagACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgc    >  5:1606829/1‑151 (MQ=255)

                                                                                                                                               gagaCAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCcgccagcgcc   >  3:1034821/1‑151 (MQ=255)

AAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCA  >  NC_000913/1358712‑1359006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`