Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 88,571 G→A V182M (GTG→ATG)  cra → transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,5710GA96.8% 1434.2 / 22.5 433V182M (GTG→ATG) cratranscriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base G (8/6);  new base A (200/217);  total (210/223)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 433 total aligned reads displayed.

TTTTTATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAA  >  NC_000913/88423‑88718
                                                                                                                                                    |                                                                                                                                                   
tttttATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATg                                                                                                                                                   >  2:1278197/1‑151 (MQ=255)
 ttttATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATg                                                                                                                                                   <  8:1217036/150‑1 (MQ=255)
  tttATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCt                                                                                                                                                 >  3:1253436/1‑151 (MQ=255)
   ttATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCtt                                                                                                                                                >  4:1620169/1‑151 (MQ=255)
     aTCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCttt                                                                                                                                               <  8:50815/150‑1 (MQ=255)
     aTCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCttt                                                                                                                                               >  8:1697308/1‑150 (MQ=255)
     aTCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTa                                                                                                                                              <  8:1002867/151‑1 (MQ=255)
          cGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTg                                                                                                                                         <  7:352125/151‑1 (MQ=255)
            cTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGt                                                                                                                                       >  1:733750/1‑151 (MQ=255)
              gggCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTgc                                                                                                                                     <  1:333691/151‑1 (MQ=255)
                  tAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCt                                                                                                                                  >  5:419358/1‑150 (MQ=255)
                    aCGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTAcc                                                                                                                               <  8:809236/151‑1 (MQ=255)
                       aCCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGa                                                                                                                            <  3:1035141/151‑1 (MQ=255)
                         ccGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGc                                                                                                                          <  8:657981/151‑1 (MQ=255)
                          cGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGCCGATGCTTTATCTTGGTGCGCTACCGGAGCt                                                                                                                         >  8:1442950/1‑151 (MQ=255)
                          cGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCt                                                                                                                         >  7:472530/1‑151 (MQ=255)
                          cGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCt                                                                                                                         <  7:150582/151‑1 (MQ=255)
                           gTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCtt                                                                                                                        >  3:726739/1‑151 (MQ=255)
                             tCCCGATTGTCGCTCTGGACAGCGCCCTCGATCGTTAACACTTCACCATAGTTGTTGGTTCAGATCAGTATTATGCCTAAATGCTGGCGGAATAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCTCTACCGTAGCttt                                                                                                                       <  8:105772/150‑1 (MQ=255)
                              cccGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTc                                                                                                                      <  4:279457/150‑1 (MQ=255)
                               ccGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTg                                                                                                                    >  1:635899/1‑151 (MQ=255)
                                   ttGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGCTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTc                                                                                                                  >  3:458518/1‑149 (MQ=255)
                                    tGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATGTTGGTGCGCTACCGGAGCTTTCTGTCAg                                                                                                                <  4:799323/150‑1 (MQ=255)
                                     gTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCt                                                                                                              <  8:1628956/151‑1 (MQ=255)
                                     gTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCt                                                                                                              >  7:1148546/1‑151 (MQ=255)
                                     gTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCt                                                                                                              <  7:779061/151‑1 (MQ=255)
                                          gcTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTg                                                                                                         >  5:1593238/1‑151 (MQ=255)
                                             ggACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGc                                                                                                        <  4:957422/149‑1 (MQ=255)
                                                ccGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGa                                                                                                    >  1:1105442/1‑150 (MQ=255)
                                                 cgcgCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAAc                                                                                                  <  5:1216562/151‑1 (MQ=255)
                                                    gcCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAGGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAg                                                                                               <  7:2774/151‑1 (MQ=255)
                                                    gcCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACa                                                                                                 >  2:556030/1‑149 (MQ=255)
                                                         cGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGtt                                                                                            <  1:1046787/149‑1 (MQ=255)
                                                         cGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTc                                                                                          >  6:60415/1‑151 (MQ=255)
                                                          gATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTcc                                                                                         >  4:1070337/1‑151 (MQ=255)
                                                            tCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGATTTACGTAAGTTTCCAGCAGAGACGATGATATATCTTGGTGCGATAACGGAGATTTATGTCAGCTTCCTGCTTGAACAAGGTTTCCgg                                                                                       >  1:533249/1‑150 (MQ=255)
                                                             cGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGGTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                       >  5:979608/1‑150 (MQ=255)
                                                             cGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                       <  7:1304277/150‑1 (MQ=255)
                                                             cGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                       >  8:691532/1‑150 (MQ=255)
                                                             cGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTa                                                                                      <  4:492358/151‑1 (MQ=255)
                                                               tGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACt                                                                                    >  4:1344447/1‑151 (MQ=255)
                                                                gAACACTTCACCAGCGGGGTTGGTGCCGATCAGGATGATGCCGAAATCCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCGTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACt                                                                                    <  6:1249231/150‑1 (MQ=255)
                                                                   cacTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCt                                                                                <  1:1489164/151‑1 (MQ=255)
                                                                   cacTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCt                                                                                <  4:194288/151‑1 (MQ=255)
                                                                    acTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTg                                                                               <  2:1646018/151‑1 (MQ=255)
                                                                    acTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTg                                                                               >  1:1252514/1‑151 (MQ=255)
                                                                        cACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGaa                                                                            <  5:379850/150‑1 (MQ=255)
                                                                          ccAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGaa                                                                            >  4:1423087/1‑148 (MQ=255)
                                                                           cAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgat                                                                        <  1:420684/151‑1 (MQ=255)
                                                                              cGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAgatgat                                                                     <  1:1458166/151‑1 (MQ=255)
                                                                                tggttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGACGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAATATTATcc                                                                   >  8:766040/1‑151 (MQ=255)
                                                                                 ggttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATcc                                                                   <  3:249152/150‑1 (MQ=255)
                                                                                  gttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGCCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGCTGCTCggc                                                                 >  2:312003/1‑151 (MQ=255)
                                                                                        gCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGaa                                                            <  5:1434675/150‑1 (MQ=255)
                                                                                        gCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAg                                                           <  1:1537956/151‑1 (MQ=255)
                                                                                          cGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTg                                                         >  2:780991/1‑151 (MQ=255)
                                                                                          cGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTg                                                         >  2:1552152/1‑151 (MQ=255)
                                                                                          cGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTg                                                         >  5:1368328/1‑151 (MQ=255)
                                                                                          cGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTg                                                         >  7:1469565/1‑151 (MQ=255)
                                                                                          cGAGCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTg                                                         <  5:210330/151‑1 (MQ=255)
                                                                                           gATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGc                                                        >  3:546362/1‑151 (MQ=255)
                                                                                            aTCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCa                                                       <  3:1387297/151‑1 (MQ=255)
                                                                                              cAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCAt                                                      <  5:6738/150‑1 (MQ=255)
                                                                                               aGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCAttt                                                    <  5:775127/151‑1 (MQ=255)
                                                                                                ggATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTc                                                   <  7:1598012/151‑1 (MQ=255)
                                                                                                ggATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTc                                                   >  5:1675609/1‑151 (MQ=255)
                                                                                                  atgatgCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCt                                                 >  6:763645/1‑151 (MQ=255)
                                                                                                     atgCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGt                                               >  5:1095624/1‑150 (MQ=255)
                                                                                                       gCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATg                                            >  6:392490/1‑151 (MQ=255)
                                                                                                           aaaTGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCa                                         >  6:1519890/1‑150 (MQ=255)
                                                                                                             aTGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAAc                                       >  2:236817/1‑150 (MQ=255)
                                                                                                               gCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAg                                     <  8:23386/150‑1 (MQ=255)
                                                                                                                cTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCt                                   >  8:386030/1‑151 (MQ=255)
                                                                                                                 tGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCt                                   >  1:138605/1‑150 (MQ=255)
                                                                                                                  ggCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTa                                  <  7:888861/150‑1 (MQ=255)
                                                                                                                  ggCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTAt                                 <  5:473021/151‑1 (MQ=255)
                                                                                                                   gCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATg                                >  4:417763/1‑151 (MQ=255)
                                                                                                                    cGGAAGAGTTACGTAAGCTTCCCGCCGCGACGATGCTTTATCTTGGGGCGCTACCGGCGCGTTCTGTCAGCTTCCTGCGTGAACTAGGTTGGCGTACTGCCTGGAAAGATGATCCGCGCGAAGGGCATTTCCTGTATGCCAACAGCTATGa                               <  2:65854/151‑1 (MQ=255)
                                                                                                                     ggAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAg                              <  2:938390/151‑1 (MQ=255)
                                                                                                                       aaGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCg                            <  1:1234983/151‑1 (MQ=255)
                                                                                                                        agagTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCGTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCgg                           <  8:133838/151‑1 (MQ=255)
                                                                                                                          agTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCgggc                         >  3:1190196/1‑150 (MQ=255)
                                                                                                                           gTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAg                        <  5:731708/151‑1 (MQ=255)
                                                                                                                              aCGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGGCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAggc                      <  6:1117639/150‑1 (MQ=255)
                                                                                                                               cGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAggcgg                    >  3:847501/1‑151 (MQ=255)
                                                                                                                                  aaGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCt                  <  8:322414/150‑1 (MQ=255)
                                                                                                                                      ttCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGccc              <  4:1299677/150‑1 (MQ=255)
                                                                                                                                       tCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGccc              >  2:1315644/1‑149 (MQ=255)
                                                                                                                                       tCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTGCTGTCAGCTTCCTGCGTGAACAAGGTGTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCa             <  6:529090/150‑1 (MQ=255)
                                                                                                                                        cccGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGt           >  6:1594639/1‑151 (MQ=255)
                                                                                                                                         ccgccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGtt          <  2:431027/151‑1 (MQ=255)
                                                                                                                                          cgccgAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGtt          >  3:961687/1‑150 (MQ=255)
                                                                                                                                          cgccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGtt          >  2:112503/1‑150 (MQ=255)
                                                                                                                                           gccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAt        >  1:946639/1‑151 (MQ=255)
                                                                                                                                            ccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAtt       <  8:590042/151‑1 (MQ=255)
                                                                                                                                              gagaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCg     <  4:1017030/151‑1 (MQ=255)
                                                                                                                                               agaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGa    <  6:917528/151‑1 (MQ=255)
                                                                                                                                               agaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTACCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAGTCg     >  2:1419654/1‑150 (MQ=255)
                                                                                                                                                gaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGaa   <  3:1543282/151‑1 (MQ=255)
                                                                                                                                                 aCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGaa   >  4:638988/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTTTATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAA  >  NC_000913/88423‑88718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: