Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,994,637 A→G Y24H (TAC→CAC)  uvrC ← excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,994,6370AG98.8% 1596.2 / 1.4 426Y24H (TAC→CAC) uvrCexcinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base G (219/200);  total (223/203)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01

Only 100 of 427 total aligned reads displayed.

TGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTT  >  NC_000913/1994490‑1994784
                                                                                                                                                   |                                                                                                                                                   
tgtgAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCAt                                                                                                                                                  >  1:379515/1‑151 (MQ=255)
 gtgAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCAt                                                                                                                                                  <  2:393744/150‑1 (MQ=255)
   gAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGc                                                                                                                                                <  4:902065/150‑1 (MQ=255)
    aGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCg                                                                                                                                               >  8:1208436/1‑150 (MQ=255)
       aaCCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATa                                                                                                                                            >  5:1640342/1‑150 (MQ=255)
        aCCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATaaa                                                                                                                                          <  7:1280169/151‑1 (MQ=255)
             tACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGcc                                                                                                                                      <  3:307749/150‑1 (MQ=255)
             tACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCct                                                                                                                                     >  5:1068887/1‑151 (MQ=255)
              aCATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCct                                                                                                                                     <  4:1588216/150‑1 (MQ=255)
              aCATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCct                                                                                                                                     <  2:554762/150‑1 (MQ=255)
                  gaaTTTGCTGGAGCTGTGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggct                                                                                                                                 <  6:296533/149‑1 (MQ=255)
                  cAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggctg                                                                                                                                >  3:152386/1‑151 (MQ=255)
                  cAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggctg                                                                                                                                <  5:1551575/151‑1 (MQ=255)
                  cAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggct                                                                                                                                 <  8:1610525/150‑1 (MQ=255)
                        gCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCctggctggctgg                                                                                                                           <  3:753875/150‑1 (MQ=255)
                          tGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGtt                                                                                                                         >  6:97812/1‑150 (MQ=255)
                           ggATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTa                                                                                                                        <  1:139373/150‑1 (MQ=255)
                           ggATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTAc                                                                                                                       <  6:550826/151‑1 (MQ=255)
                           ggATCTGGGCGACCAGCGCTGCGGTGTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGGTTTTTCAGGGCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTAc                                                                                                                       <  1:603718/151‑1 (MQ=255)
                           ggAGCTGGGCGACCAGCGCTGCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTAc                                                                                                                       <  6:84566/151‑1 (MQ=255)
                            gATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACg                                                                                                                      >  3:620975/1‑151 (MQ=255)
                             aTCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACgg                                                                                                                     >  5:623087/1‑151 (MQ=255)
                             aTCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACgg                                                                                                                     >  2:618423/1‑151 (MQ=255)
                                 gggCGACCTGCGCTTCGGTTTTGCGGTAAGCGAGGTTGCTACGTAAATAGCTGGAAAGCCGTTTTTTCTGGTCTTTCTCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGttt                                                                                                                   <  1:454826/149‑1 (MQ=255)
                                 gggCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGtttt                                                                                                                 <  8:70750/151‑1 (MQ=255)
                                     gACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTaa                                                                                                              >  5:1498198/1‑150 (MQ=255)
                                     gACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTaa                                                                                                              >  7:1435166/1‑150 (MQ=255)
                                      aCCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTaaaa                                                                                                            <  1:1645488/151‑1 (MQ=255)
                                         cgcgcTTCTGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAACGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAAGCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACg                                                                                                         <  1:243998/150‑1 (MQ=255)
                                         aGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTCGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACg                                                                                                         <  7:61317/151‑1 (MQ=255)
                                           cgcTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCt                                                                                                       <  7:1067002/151‑1 (MQ=255)
                                               tCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCtttt                                                                                                    <  1:983265/150‑1 (MQ=255)
                                               tCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCtttt                                                                                                    <  5:839432/150‑1 (MQ=255)
                                                gggTTTTGCGCGATGCGAGGGGGCTACGGAAATTGCTGGAAAGCCGTTTTTTCAGGGCTTTCGCGTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGc                                                                                                  <  4:1291094/150‑1 (MQ=255)
                                                cGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGTTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGc                                                                                                  <  7:743774/151‑1 (MQ=255)
                                                 ggTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCg                                                                                                 <  6:77264/151‑1 (MQ=255)
                                                 ggTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCg                                                                                                 >  8:868680/1‑151 (MQ=255)
                                                 ggTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCg                                                                                                 <  3:1093324/151‑1 (MQ=255)
                                                   ttttGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTc                                                                                               >  5:1340376/1‑151 (MQ=255)
                                                    tttGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTc                                                                                               <  1:1523391/150‑1 (MQ=255)
                                                    tttGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTc                                                                                               <  2:201544/150‑1 (MQ=255)
                                                     ttGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCa                                                                                              <  6:1611644/150‑1 (MQ=255)
                                                     ttGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCa                                                                                              <  8:1091579/150‑1 (MQ=255)
                                                         gcgAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTg                                                                                         <  1:1200266/151‑1 (MQ=255)
                                                           gAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAt                                                                                       >  3:1151658/1‑151 (MQ=255)
                                                                gAGGTTGCTACGGAAATAGCTGGAAACCCGTTTTTTCAGCTCTTTCGCTTTGCCGACATACATAACCGTACCACCAGCATCCTGCATGCGATAAACGCATGGCAGGCTGCTTACGCTTTTTAAAAACTCTTATCCATAAAACAGAtcact                                                                                   >  2:7719/1‑150 (MQ=255)
                                                                 aGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAtcactca                                                                                 <  4:1477474/151‑1 (MQ=255)
                                                                 aGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAtcactc                                                                                  >  2:843137/1‑150 (MQ=255)
                                                                   gTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATcactcact                                                                               <  6:1066007/151‑1 (MQ=255)
                                                                       cTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACt                                                                           >  5:550731/1‑151 (MQ=255)
                                                                         aCGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACtt                                                                          <  6:1500625/150‑1 (MQ=255)
                                                                         aCGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACt                                                                           <  2:1083980/149‑1 (MQ=255)
                                                                         aCGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTg                                                                         >  6:1005266/1‑151 (MQ=255)
                                                                              aaTAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAAt                                                                    <  3:432868/151‑1 (MQ=255)
                                                                               aTAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATATTg                                                                   >  4:349962/1‑151 (MQ=255)
                                                                               aTAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGACTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAAATGATCACTCACTGACTTGATAATg                                                                   >  6:1337893/1‑151 (MQ=255)
                                                                                 aGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGtc                                                                 >  6:1694870/1‑151 (MQ=255)
                                                                                 aGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGt                                                                  <  7:299384/150‑1 (MQ=255)
                                                                                  gCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGtct                                                                >  8:1312859/1‑151 (MQ=255)
                                                                                    tGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTcc                                                              >  4:703818/1‑151 (MQ=255)
                                                                                     ggAAAGCCGTTTTTTCAGGTCATTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAGCACTCACTGACTTGATAATGTCTCCg                                                             <  6:425294/151‑1 (MQ=255)
                                                                                      gAAACCCGTTTTTTCAGGGCTTTGCGTTTGCCGCAAAAGATTACCGTACCCCCACCATCGTGCATGCGATAAACGCCCGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGc                                                            <  4:232985/151‑1 (MQ=255)
                                                                                       aaaGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCa                                                           <  7:1099106/151‑1 (MQ=255)
                                                                                         aGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCAtt                                                         >  3:141678/1‑151 (MQ=255)
                                                                                             gTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTacac                                                     >  6:1166455/1‑151 (MQ=255)
                                                                                              ttttttCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTacac                                                     <  1:732569/150‑1 (MQ=255)
                                                                                               tttttCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTAcaca                                                    <  5:1151764/150‑1 (MQ=255)
                                                                                                ttttCAGGTCTTTCGCTTTGCCGACATANANANCCNTNCNANNNNCANNNNNCANGCGNTAANCGCCNGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTacac                                                     >  5:399175/1‑148 (MQ=255)
                                                                                                ttttCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTacac                                                     >  7:427214/1‑148 (MQ=255)
                                                                                                ttttCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAg                                                   <  1:1090292/150‑1 (MQ=255)
                                                                                                   tCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGAcc                                                >  4:1194658/1‑150 (MQ=255)
                                                                                                       gTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATgga                                           <  8:296458/151‑2 (MQ=255)
                                                                                                           ttCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGa                                         <  3:96457/149‑1 (MQ=255)
                                                                                                             cGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTg                                     <  8:235650/151‑1 (MQ=255)
                                                                                                              gCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGc                                    >  2:1237177/1‑151 (MQ=255)
                                                                                                              gCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGc                                    >  3:1642051/1‑151 (MQ=255)
                                                                                                               cTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGcc                                   >  8:1249778/1‑151 (MQ=255)
                                                                                                               cTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGcc                                   >  7:857831/1‑151 (MQ=255)
                                                                                                                tttGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGcc                                   >  2:323370/1‑150 (MQ=255)
                                                                                                                 ttGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGcc                                   >  1:1138319/1‑149 (MQ=255)
                                                                                                                 tggCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAACCGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCa                                  <  6:227667/148‑1 (MQ=255)
                                                                                                                    ccGACATAGATAACCGTACCACCATCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTCACTTGATAATCTATCAGCATTACAAAGACCATGGCGAATTGCAAGGTg                              >  1:1273893/1‑151 (MQ=255)
                                                                                                                     cGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGa                             <  1:867333/151‑1 (MQ=255)
                                                                                                                       aCATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGt                           <  1:506460/151‑1 (MQ=255)
                                                                                                                       aCATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGt                           >  8:803786/1‑151 (MQ=255)
                                                                                                                         aTAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCAGCATTACACAGACCATGGCGAATTGCCAGGTGAGTc                          >  2:71352/1‑150 (MQ=255)
                                                                                                                           aGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGc                       >  4:1342939/1‑151 (MQ=255)
                                                                                                                             aTAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTc                     <  2:153187/151‑1 (MQ=255)
                                                                                                                              tAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGTTCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTc                     >  5:286509/1‑150 (MQ=255)
                                                                                                                               aaCCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACTGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCa                    <  4:415630/150‑1 (MQ=255)
                                                                                                                               aaCCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCaa                   <  7:162144/151‑1 (MQ=255)
                                                                                                                                 ccGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACa                 <  2:1413531/151‑1 (MQ=255)
                                                                                                                                 ccGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACa                 <  8:1499877/151‑1 (MQ=255)
                                                                                                                                  cGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACAt                <  8:1381896/151‑1 (MQ=255)
                                                                                                                                   gTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATc               <  8:375909/151‑1 (MQ=255)
                                                                                                                                     accaccAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACACACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGc             <  8:507423/151‑1 (MQ=255)
                                                                                                                                         ccaGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATg         <  5:1456134/151‑1 (MQ=255)
                                                                                                                                          caGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGNATTGCCAGGTGAGTCAGCTCAACATCGCCATg         >  3:1247332/1‑150 (MQ=255)
                                                                                                                                              aTCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATgt    <  7:1561662/151‑1 (MQ=255)
                                                                                                                                                cGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATgttt  <  4:816866/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTT  >  NC_000913/1994490‑1994784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: