Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,046,506 G→T V523V (GTG→GTT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,046,5060GT96.5% 1281.0 / 28.3 374V523V (GTG→GTT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (8/5);  new base T (161/198);  total (170/204)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 391 total aligned reads displayed.

GCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCG  >  NC_000913/2046358‑2046653
                                                                                                                                                    |                                                                                                                                                   
gCAAAGTGGTCACAATGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggttgt                                                                                                                                                   <  3:1175633/151‑1 (MQ=255)
gCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggttgt                                                                                                                                                   <  4:18652/151‑1 (MQ=255)
gCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATggttgt                                                                                                                                                   <  8:44613/151‑1 (MQ=255)
   aaGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGt                                                                                                                                                >  5:1486985/1‑151 (MQ=255)
       ggTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAg                                                                                                                                            <  2:762194/151‑1 (MQ=255)
          cacaACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGcc                                                                                                                                         >  1:678675/1‑150 (MQ=255)
             aaCGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCCCCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCACAGGCAAGTTGTCGAATCGTGAACAGAGCAGGGTTGTCGTTCAGGccccg                                                                                                                                      >  6:949574/1‑150 (MQ=255)
             aaCGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCt                                                                                                                                      <  8:880523/151‑1 (MQ=255)
               cGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTAc                                                                                                                                    <  8:92909/151‑1 (MQ=255)
               cGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTAc                                                                                                                                    <  5:190289/151‑1 (MQ=255)
               cGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTAc                                                                                                                                    <  7:1492533/151‑1 (MQ=255)
               cGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTAc                                                                                                                                    <  4:1187437/151‑1 (MQ=255)
                gggTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACg                                                                                                                                   >  6:1393883/1‑151 (MQ=255)
                   tAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCt                                                                                                                                 >  5:1358289/1‑150 (MQ=255)
                     aaGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGCTAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTa                                                                                                                                >  6:423553/1‑149 (MQ=255)
                        atatTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCa                                                                                                                           <  1:1083971/151‑1 (MQ=255)
                        atatTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCAGGCCGATTGAACTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGCACAGACCATGGGTGTCGTTCAGGCACCTACGCTAAccc                                                                                                                            >  2:535403/1‑150 (MQ=255)
                            tCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGaa                                                                                                                        >  3:947637/1‑150 (MQ=255)
                               ggTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGaa                                                                                                                        >  1:353413/1‑147 (MQ=255)
                                  tACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTc                                                                                                                  <  5:955795/150‑1 (MQ=255)
                                    cccTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTc                                                                                                               <  8:1669598/151‑1 (MQ=255)
                                     ccTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCg                                                                                                              <  4:584607/151‑1 (MQ=255)
                                     ccTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCg                                                                                                              >  1:1665708/1‑151 (MQ=255)
                                       tGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGt                                                                                                            >  6:30992/1‑151 (MQ=255)
                                        gacGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGAGTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCGAGAAAGATTCCTCGGTa                                                                                                           <  2:318342/149‑1 (MQ=255)
                                         ccGGCTTACCGGTTCACCAGTACGCCTGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTac                                                                                                          <  3:1608103/151‑2 (MQ=255)
                                         ccGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTAt                                                                                                          >  7:1306476/1‑151 (MQ=255)
                                          cGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATc                                                                                                         <  8:273718/151‑1 (MQ=255)
                                           ggCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAAGAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCGTCGGTATCg                                                                                                        >  6:555206/1‑151 (MQ=255)
                                           ggCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCg                                                                                                        <  7:937747/151‑1 (MQ=255)
                                            gCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGt                                                                                                       <  3:455208/151‑1 (MQ=255)
                                               tACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTa                                                                                                     >  2:1260111/1‑150 (MQ=255)
                                               tACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTa                                                                                                     <  4:408014/150‑1 (MQ=255)
                                                aCCGGTTCACCAGTACGACAGAAACCGATAACACCTGGCCGATTGAAGTCACAGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACATAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTa                                                                                                     >  6:1484566/1‑149 (MQ=255)
                                                 ccGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGt                                                                                                  <  1:1559/151‑1 (MQ=255)
                                                 ccGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCT‑AGCCAGAAAGATTCCTCGGTATCGTTAAGt                                                                                                  >  5:433916/1‑150 (MQ=255)
                                                  cGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGt                                                                                                  >  7:1204892/1‑150 (MQ=255)
                                                  cGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGt                                                                                                  >  4:1443828/1‑150 (MQ=255)
                                                   ggTTCACCAGTACGCCATAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTAc                                                                                                <  6:161849/151‑1 (MQ=255)
                                                      tCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTAccc                                                                                              <  5:1197720/150‑1 (MQ=255)
                                                      tCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTAccc                                                                                              <  2:678142/150‑1 (MQ=255)
                                                        aCCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCaaa                                                                                           <  8:785479/151‑1 (MQ=255)
                                                           aGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCTCCGAAGATGTGAAAGTCAATTTGTCGAATCCTGAACAGAGCATGGTTGTCGTTCAGGCACCAAAGATAAGGCAGAAAGATTCCTCGGTATCGTAAAGTACCCAAACa                                                                                         >  2:846407/1‑150 (MQ=255)
                                                              aCGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTg                                                                                      >  5:706661/1‑150 (MQ=255)
                                                               cGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGaa                                                                                    <  4:1556347/151‑1 (MQ=255)
                                                               cGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGaa                                                                                    <  8:1511967/151‑1 (MQ=255)
                                                                gCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAAc                                                                                   >  3:1484292/1‑151 (MQ=255)
                                                                  cAGAAACCGATAACAGCTGGCAGATTGAAGTCACCGCCGAAGCTGTCAAAGGCAAGTTGTCGAATCCTGAACAGAGCATGGGTGTCGTTCAGGCACCTAAGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAAcgc                                                                                 <  1:131643/151‑1 (MQ=255)
                                                                   aGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAAcgcg                                                                                >  3:1021263/1‑151 (MQ=255)
                                                                       aCCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGAtt                                                                            >  4:919205/1‑151 (MQ=255)
                                                                        ccGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTc                                                                           >  3:86378/1‑151 (MQ=255)
                                                                        ccGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTc                                                                           <  1:551278/151‑1 (MQ=255)
                                                                        ccGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTAGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTc                                                                           >  1:247820/1‑151 (MQ=255)
                                                                            tAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATACCCAt                                                                       >  5:286559/1‑151 (MQ=255)
                                                                             aaCACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCAtt                                                                      >  1:822262/1‑151 (MQ=255)
                                                                             aaCACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCAt                                                                       <  7:1437899/150‑1 (MQ=255)
                                                                               cacCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTc                                                                     >  5:1388256/1‑150 (MQ=255)
                                                                                 ccTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAAc                                                                  <  6:882425/151‑1 (MQ=255)
                                                                                  cTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAAcc                                                                 >  8:111164/1‑151 (MQ=255)
                                                                                   tGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCg                                                                >  3:272249/1‑151 (MQ=255)
                                                                                    ggCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAAcc                                                                 <  6:1162147/149‑1 (MQ=255)
                                                                                    ggCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGc                                                               >  2:139065/1‑151 (MQ=255)
                                                                                     gCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGcc                                                              <  8:1242687/151‑1 (MQ=255)
                                                                                      ccGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCgtc                                                              >  4:847437/1‑148 (MQ=255)
                                                                                       cGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCcac                                                            >  2:418865/1‑151 (MQ=255)
                                                                                          ttGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACt                                                         >  5:1172404/1‑151 (MQ=255)
                                                                                           tGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACt                                                         <  8:1341959/150‑1 (MQ=255)
                                                                                            gAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATGGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGa                                                       <  7:861481/151‑1 (MQ=255)
                                                                                            gAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTg                                                        <  1:715437/150‑1 (MQ=255)
                                                                                            gAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGa                                                       <  1:1053135/151‑1 (MQ=255)
                                                                                                 cACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACttt                                                   <  2:1064460/150‑1 (MQ=255)
                                                                                                    cgccgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTAtt                                               >  3:369000/1‑151 (MQ=255)
                                                                                                      ccgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCTACACTGACTTTTATTgc                                             <  2:1677116/151‑1 (MQ=255)
                                                                                                       cgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTgcg                                            >  3:567292/1‑151 (MQ=255)
                                                                                                       cgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTgcg                                            >  7:622381/1‑151 (MQ=255)
                                                                                                       cgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTgcg                                            >  5:898306/1‑151 (MQ=255)
                                                                                                         aaGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTgcgc                                           >  5:650326/1‑150 (MQ=255)
                                                                                                              gTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCatgatg                                     <  5:352423/151‑1 (MQ=255)
                                                                                                                cAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATgc                                    >  2:1205330/1‑150 (MQ=255)
                                                                                                                   aGGCAATTTGTCGAATCGTGAACAGAGCATGGGTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATgcagca                                <  5:1021178/151‑1 (MQ=255)
                                                                                                                    ggCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCACATGATgcagc                                 >  7:1054056/1‑149 (MQ=255)
                                                                                                                     gCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAgg                              <  1:1489530/151‑1 (MQ=255)
                                                                                                                     gCAATTTGTCGAATCGTGAACAGAGAATGGTTTTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCAATTCAACCGCCACACTGACTTTTATTGCGCATGATgcagcatt                              >  4:965947/1‑149 (MQ=255)
                                                                                                                        aTTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTaa                           <  6:1226047/151‑1 (MQ=255)
                                                                                                                        aTTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCAGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTaa                           <  7:727619/151‑1 (MQ=255)
                                                                                                                                aaTCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCtgttg                   <  4:323982/151‑1 (MQ=255)
                                                                                                                                  tCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTc                 >  5:161042/1‑151 (MQ=255)
                                                                                                                                   cGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTc                 <  3:714298/150‑1 (MQ=255)
                                                                                                                                    gTGAACAGAGCATGGTTGTCGTTCAGGCACCTATGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCgg               >  4:717750/1‑151 (MQ=255)
                                                                                                                                    gTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCgg               <  8:349277/151‑1 (MQ=255)
                                                                                                                                     tGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCggg              >  8:1417804/1‑151 (MQ=255)
                                                                                                                                     tGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCggg              >  2:1632395/1‑151 (MQ=255)
                                                                                                                                      gAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAAGATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGc             <  7:154458/151‑1 (MQ=255)
                                                                                                                                      gAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCggg              <  2:1492515/150‑1 (MQ=255)
                                                                                                                                         cAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTgg          <  4:499361/151‑1 (MQ=255)
                                                                                                                                           gagCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTg        <  7:415352/151‑1 (MQ=255)
                                                                                                                                                 ggttgtCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCtctc   <  4:1364494/150‑1 (MQ=255)
                                                                                                                                                 ggttgtCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCtctc   <  6:771684/150‑1 (MQ=255)
                                                                                                                                                 ggttgtCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCg  >  1:1220155/1‑151 (MQ=255)
                                                                                                                                                 ggttgtCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCg  <  5:1136445/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCG  >  NC_000913/2046358‑2046653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑