Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 113,695 G→A W84* (TGG→TGA)  guaC → GMP reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913113,6950GA96.9% 1426.1 / 21.1 426W84* (TGG→TGA) guaCGMP reductase
Reads supporting (aligned to +/- strand):  ref base G (2/11);  new base A (203/209);  total (205/221)
Fisher's exact test for biased strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 426 total aligned reads displayed.

CACCTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGT  >  NC_000913/113548‑113842
                                                                                                                                                   |                                                                                                                                                   
cACCTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACa                                                                                                                                                   <  5:453524/150‑1 (MQ=255)
cACCTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACa                                                                                                                                                   >  7:582975/1‑150 (MQ=255)
cACCTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACa                                                                                                                                                   >  6:715550/1‑150 (MQ=255)
  ccTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGc                                                                                                                                                <  5:1094194/151‑1 (MQ=255)
  ccTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGc                                                                                                                                                <  7:162123/151‑1 (MQ=255)
      cAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGtt                                                                                                                                             <  6:1188368/150‑1 (MQ=255)
       aaaCATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGttt                                                                                                                                            >  6:1610012/1‑150 (MQ=255)
       aaaCATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTa                                                                                                                                           <  8:1171366/151‑1 (MQ=255)
          cATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATca                                                                                                                                        <  3:1047467/151‑1 (MQ=255)
          cATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATca                                                                                                                                        <  8:750226/151‑1 (MQ=255)
           aTTCAGGTCAGAGCTGGTCCGGCGTTCAGATTATCGCCGCATAGATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTTCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATcaa                                                                                                                                       <  1:999266/151‑1 (MQ=255)
              cAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATcaacaa                                                                                                                                    <  8:138555/151‑1 (MQ=255)
                   cAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAAttctt                                                                                                                               <  2:1414488/151‑1 (MQ=255)
                     gagCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTcc                                                                                                                             >  4:238247/1‑151 (MQ=255)
                     gagCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTcc                                                                                                                             <  6:1049487/151‑1 (MQ=255)
                      agCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCg                                                                                                                            <  5:1681226/151‑1 (MQ=255)
                      agCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCGTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCg                                                                                                                            >  1:694064/1‑151 (MQ=255)
                          gggccGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTg                                                                                                                         <  1:16565/147‑1 (MQ=255)
                          ggaccGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGa                                                                                                                        <  1:1238306/148‑1 (MQ=255)
                           gTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGAt                                                                                                                       >  1:1007767/1‑151 (MQ=255)
                           gTCCGGCGTGACTATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTTCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGAt                                                                                                                       <  1:177522/151‑1 (MQ=255)
                            tCCGTCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGAtg                                                                                                                      >  3:682181/1‑151 (MQ=255)
                            tCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGAtg                                                                                                                      >  6:691728/1‑151 (MQ=255)
                                 cGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTg                                                                                                                 >  6:431067/1‑151 (MQ=255)
                                   tGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGa                                                                                                                >  4:1094543/1‑150 (MQ=255)
                                      cGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACa                                                                                                            <  4:468986/151‑1 (MQ=255)
                                            tCGCCGCAAATATGGACACCGTATGCACAGTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGAt                                                                                                      >  1:846873/1‑151 (MQ=255)
                                              gccgcAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATgg                                                                                                    >  3:564429/1‑151 (MQ=255)
                                              gccgcAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATgg                                                                                                    >  8:533683/1‑151 (MQ=255)
                                               ccgcAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGt                                                                                                   <  1:477245/151‑1 (MQ=255)
                                               ccgcAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGt                                                                                                   <  7:1040350/151‑1 (MQ=255)
                                                     atatGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTAc                                                                                             >  5:74571/1‑151 (MQ=255)
                                                     atatGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTAc                                                                                             >  8:642368/1‑151 (MQ=255)
                                                       atGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCg                                                                                           >  4:779553/1‑151 (MQ=255)
                                                       atGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCg                                                                                           <  8:1521557/151‑1 (MQ=255)
                                                       atGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCg                                                                                           <  6:535275/151‑1 (MQ=255)
                                                            cacCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACg                                                                                      <  5:1418845/151‑1 (MQ=255)
                                                              ccGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTc                                                                                    >  1:1127531/1‑151 (MQ=255)
                                                              ccGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTc                                                                                    >  5:1059431/1‑151 (MQ=255)
                                                                  aGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGAt                                                                                >  6:357433/1‑151 (MQ=255)
                                                                  aGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGAt                                                                                <  1:1578433/151‑1 (MQ=255)
                                                                    gCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTGTGATgg                                                                              >  7:668009/1‑150 (MQ=255)
                                                                     cacaTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCg                                                                             <  1:1458521/151‑1 (MQ=255)
                                                                        aTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCTGTACGTCTGATGCGGAt                                                                          >  7:34950/1‑151 (MQ=255)
                                                                        aTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGAt                                                                          <  7:841052/151‑1 (MQ=255)
                                                                        aTTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGAt                                                                          <  3:1528231/151‑1 (MQ=255)
                                                                         ttttCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGAt                                                                          <  1:58068/150‑1 (MQ=255)
                                                                          tttCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGAtt                                                                         <  4:873933/150‑1 (MQ=255)
                                                                           ttCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTc                                                                       >  2:1090464/1‑151 (MQ=255)
                                                                               aTGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCg                                                                      <  3:201525/148‑1 (MQ=255)
                                                                                  gCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGaaaaa                                                                 >  4:410989/1‑150 (MQ=255)
                                                                                   ccTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAAc                                                               <  2:1533749/151‑1 (MQ=255)
                                                                                   ccTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAAc                                                               <  8:44794/151‑1 (MQ=255)
                                                                                    ctctGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGCCTGATGCGGATTTCGAAAAAACt                                                              >  6:1342476/1‑151 (MQ=255)
                                                                                       tGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTaa                                                            >  1:1176550/1‑150 (MQ=255)
                                                                                        gcgcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTaa                                                            <  7:1264242/149‑1 (MQ=255)
                                                                                        gcgcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTTATGGTTTCTACAGGTACGTCTGATGACGATTAAGAAAAAACTaaa                                                           >  2:439675/1‑150 (MQ=255)
                                                                                        gcgcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTaaa                                                           >  3:859123/1‑150 (MQ=255)
                                                                                         cgcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAAc                                                          >  3:997486/1‑150 (MQ=255)
                                                                                          gcTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAg                                                        <  5:82574/151‑1 (MQ=255)
                                                                                           cTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAAc                                                          >  3:59047/1‑148 (MQ=255)
                                                                                           cTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTCAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAACCTAAAc                                                          >  3:431508/1‑148 (MQ=255)
                                                                                            tGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAAc                                                          >  3:967895/1‑147 (MQ=255)
                                                                                             ggCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGAtt                                                     >  8:860663/1‑151 (MQ=255)
                                                                                             ggCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGAtt                                                     <  7:759624/151‑1 (MQ=255)
                                                                                              gCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATtc                                                    <  4:955773/151‑1 (MQ=255)
                                                                                              gCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATtc                                                    <  2:982223/151‑1 (MQ=255)
                                                                                                 tcttTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATtct                                                   <  5:237275/149‑1 (MQ=255)
                                                                                                 tcttTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCg                                                 >  7:1454892/1‑151 (MQ=255)
                                                                                                    tttGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGAcc                                              >  6:154597/1‑151 (MQ=255)
                                                                                                    tttGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGAc                                               <  8:1078784/150‑1 (MQ=255)
                                                                                                      tGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCt                                             <  2:753140/150‑1 (MQ=255)
                                                                                                          atTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAAcc                                        >  5:92837/1‑151 (MQ=255)
                                                                                                          atTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAAcc                                        <  7:1320826/151‑1 (MQ=255)
                                                                                                           ttttGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAAccc                                       >  2:519509/1‑151 (MQ=255)
                                                                                                             ttGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCg                                      <  3:743700/150‑1 (MQ=255)
                                                                                                             ttGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCg                                      <  4:1356951/150‑1 (MQ=255)
                                                                                                              tGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGc                                    >  1:349957/1‑151 (MQ=255)
                                                                                                                 ctgctgTACATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCAtt                                 >  2:266553/1‑151 (MQ=255)
                                                                                                                       tgCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACtt                           >  1:279663/1‑151 (MQ=255)
                                                                                                                       tgCATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACt                            <  3:1194269/150‑1 (MQ=255)
                                                                                                                         cATAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTGTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCg                         <  4:1281238/151‑1 (MQ=255)
                                                                                                                          aTAAACACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGt                        >  3:27216/1‑151 (MQ=255)
                                                                                                                            aaaCACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGttt                      >  2:89689/1‑151 (MQ=255)
                                                                                                                            aaaCACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGttt                      <  2:1220282/151‑1 (MQ=255)
                                                                                                                            aaaCACTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGttt                      >  8:1704373/1‑151 (MQ=255)
                                                                                                                              acacTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGt                    <  6:893764/151‑1 (MQ=255)
                                                                                                                              acacTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGt                    <  7:212590/151‑1 (MQ=255)
                                                                                                                              acacTATTCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCAGGCATTAAACTTCGTTTGt                    >  3:1274584/1‑151 (MQ=255)
                                                                                                                                    ttCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGAc              >  4:1644076/1‑151 (MQ=255)
                                                                                                                                     tCTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACg             >  2:172672/1‑151 (MQ=255)
                                                                                                                                      cTGTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGt            >  3:33053/1‑151 (MQ=255)
                                                                                                                                        gTCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTg           <  5:1546417/150‑1 (MQ=255)
                                                                                                                                         tCGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGc         >  6:1700256/1‑151 (MQ=255)
                                                                                                                                          cGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCg        >  5:3670/1‑151 (MQ=255)
                                                                                                                                          cGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCg        <  6:335125/151‑1 (MQ=255)
                                                                                                                                          cGAAGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCg        >  4:659608/1‑151 (MQ=255)
                                                                                                                                          cGAAGAGTGACAAGAGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGc         <  8:284683/150‑1 (MQ=255)
                                                                                                                                            aaGAGTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGa       >  2:725944/1‑150 (MQ=255)
                                                                                                                                              gagTGACAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATg    >  2:650357/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CACCTTCAAACATTCAGGTCAGAGCTGGTCCGGCGTGCCGATTATCGCCGCAAATATGGACACCGTAGGCACATTTTCTATGGCCTCTGCGCTGGCTTCTTTTGATATTTTGACTGCTGTGCATAAACACTATTCTGTCGAAGAGTGGCAAGCGTTTATCAACAATTCTTCCGCTGATGTGCTGAAACATGTGATGGTTTCTACCGGTACGTCTGATGCGGATTTCGAAAAAACTAAACAGATTCTCGACCTGAACCCGGCATTAAACTTCGTTTGTATTGACGTGGCGAATGGT  >  NC_000913/113548‑113842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑