Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,137,543 G→A intergenic (‑300/‑359) wza ← / → yegH colanic acid export protein; outer membrane auxillary lipoprotein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,137,5430GA95.8% 1572.5 / 38.5 478intergenic (‑300/‑359)wza/yegHcolanic acid export protein; outer membrane auxillary lipoprotein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (7/13);  new base A (216/242);  total (223/255)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 479 total aligned reads displayed.

TTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTT  >  NC_000913/2137395‑2137690
                                                                                                                                                    |                                                                                                                                                   
  cAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGa                                                                                                                                                 <  7:179262/151‑1 (MQ=255)
    gACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACa                                                                                                                                               <  8:760286/151‑1 (MQ=255)
    gACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACa                                                                                                                                               <  3:1607670/151‑1 (MQ=255)
        ggTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTAt                                                                                                                                           >  6:1140149/1‑151 (MQ=255)
            tttCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGt                                                                                                                                        >  4:1411916/1‑150 (MQ=255)
             ttCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGt                                                                                                                                        <  8:119756/149‑1 (MQ=255)
               cAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAAtt                                                                                                                                    <  3:1220426/151‑1 (MQ=255)
               cAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAAtt                                                                                                                                    >  4:108900/1‑151 (MQ=255)
                 ggCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGa                                                                                                                                  <  3:1678393/151‑1 (MQ=255)
                  gCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGaa                                                                                                                                 <  8:875906/151‑1 (MQ=255)
                        aGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTgg                                                                                                                           >  6:1633773/1‑151 (MQ=255)
                         gACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGc                                                                                                                          <  5:282206/151‑1 (MQ=255)
                           cacGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCtt                                                                                                                        >  2:578301/1‑151 (MQ=255)
                             cGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTc                                                                                                                      >  6:30375/1‑151 (MQ=255)
                                 aCCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCaa                                                                                                                   <  4:1600100/150‑1 (MQ=255)
                                   cGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCaataa                                                                                                                <  7:1207596/151‑1 (MQ=255)
                                   cGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCaata                                                                                                                 >  6:215776/1‑150 (MQ=255)
                                     ccccTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATg                                                                                                              <  1:545371/151‑1 (MQ=255)
                                      cccTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGc                                                                                                             >  2:633571/1‑151 (MQ=255)
                                      cccTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGc                                                                                                             <  2:1635117/151‑1 (MQ=255)
                                          ggCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagga                                                                                                         <  8:1490743/151‑1 (MQ=255)
                                           gCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagg                                                                                                          >  1:1144545/1‑149 (MQ=255)
                                            cTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCagtagg                                                                                                       >  2:493223/1‑151 (MQ=255)
                                                ttAGGTACCTATTGACTTATTTAGTTTAATTTTTCACACCCTCTAAGCATGCAGTCGTTGTTGAGAAAGGGTTATTTCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATTTTGGCTTTCCAATAATTCAGGTGGAAg                                                                                                    <  1:829498/150‑1 (MQ=255)
                                                ttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAg                                                                                                    <  6:232334/150‑1 (MQ=255)
                                                  aGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAgtg                                                                                                  <  2:996515/150‑1 (MQ=255)
                                                    gtACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTa                                                                                               <  4:240751/150‑1 (MQ=255)
                                                    cTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGtt                                                                                                >  4:913594/1‑150 (MQ=255)
                                                    cTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGtt                                                                                                >  5:1677892/1‑150 (MQ=255)
                                                      aCCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTAc                                                                                              <  2:1671396/150‑1 (MQ=255)
                                                      aCCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTAc                                                                                              >  3:93089/1‑150 (MQ=255)
                                                      aCCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACa                                                                                             <  5:345378/151‑1 (MQ=255)
                                                      aCCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACa                                                                                             <  3:392158/151‑1 (MQ=255)
                                                          aTACACTGATTTAGTTTAACTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTa                                                                                         <  2:786269/151‑1 (MQ=255)
                                                            acacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAAc                                                                                       <  6:1390261/151‑1 (MQ=255)
                                                             cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACg                                                                                      <  1:174878/151‑1 (MQ=255)
                                                             cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACg                                                                                      <  6:1005351/151‑1 (MQ=255)
                                                             cacTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACg                                                                                      >  8:452472/1‑151 (MQ=255)
                                                              acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACgg                                                                                     >  5:465630/1‑151 (MQ=255)
                                                              acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACgg                                                                                     <  2:1258046/151‑1 (MQ=255)
                                                              acTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACCGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTATCAAATAATGCAGGAGGAAGTGTTACAGCTAActg                                                                                     >  1:908490/1‑149 (MQ=255)
                                                                    ttAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGc                                                                               >  1:69819/1‑151 (MQ=255)
                                                                       gTTTAATTTTTCACACCCTCTCAGCATGCAGACGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACAATATGCTAATTGAATATTCGCTTTCCAAAAATCCAGGAGGAAGTGTTACAGCTAACCGAATAGCAgg                                                                            >  1:1476946/1‑151 (MQ=255)
                                                                        tttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGc                                                                           >  4:1338618/1‑151 (MQ=255)
                                                                         ttAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGc                                                                           >  3:706064/1‑150 (MQ=255)
                                                                              ttttCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGAt                                                                      >  7:1333043/1‑150 (MQ=255)
                                                                               tttCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAAATAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATa                                                                     >  8:322750/1‑150 (MQ=255)
                                                                                 tCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAAc                                                                   <  8:1310998/150‑1 (MQ=255)
                                                                                  cacaCCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACa                                                                  <  3:1299984/150‑1 (MQ=255)
                                                                                  cacaCCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACa                                                                  <  1:1286434/150‑1 (MQ=255)
                                                                                   acacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAt                                                                >  7:694632/1‑151 (MQ=255)
                                                                                   acacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAt                                                                <  5:1118591/151‑1 (MQ=255)
                                                                                    cacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAtt                                                               <  2:1304262/151‑1 (MQ=255)
                                                                                    cacCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAAt                                                                >  2:37571/1‑150 (MQ=255)
                                                                                     acCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTc                                                              <  7:317600/151‑1 (MQ=255)
                                                                                     acCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCATTCCAATAATGCAGCAGGAACTGTTACAGCTAACGGAATAGAAGGCAAGATAACAATTc                                                              >  6:784707/1‑151 (MQ=255)
                                                                                      cccTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCg                                                             >  1:1012692/1‑151 (MQ=255)
                                                                                      cccTATCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCg                                                             <  6:553211/151‑1 (MQ=255)
                                                                                       ccTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTATTTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCgg                                                            >  1:319519/1‑151 (MQ=255)
                                                                                            cAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAAtt                                                       <  8:559621/151‑1 (MQ=255)
                                                                                            cAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTaa                                                         <  2:1045422/149‑1 (MQ=255)
                                                                                            cAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTa                                                          <  5:869216/148‑1 (MQ=255)
                                                                                              gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTgg                                                     <  6:1081396/151‑1 (MQ=255)
                                                                                              gCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTgg                                                     <  4:1468/151‑1 (MQ=255)
                                                                                              ccATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTgg                                                     <  1:1517016/150‑1 (MQ=255)
                                                                                                   cAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTAtt                                                <  6:1243348/151‑1 (MQ=255)
                                                                                                   cAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTAtt                                                <  2:256970/151‑1 (MQ=255)
                                                                                                    aGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTAttt                                               <  7:1552043/151‑1 (MQ=255)
                                                                                                     gTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTAtttt                                              <  5:780358/151‑1 (MQ=255)
                                                                                                       gggtgttgaGAAAGTGTTATTACGGAAATTAACTTCCGAATTTAAAGTGACATTATGGTAATTGAATATTGGCTTTCCTATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATTACAATTCGGTAATTGGCTATTTTTa                                            <  8:236184/145‑1 (MQ=255)
                                                                                                       cGTTGATGATAAAGGGTTATTACGGAAATTAACTTCCAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTa                                            <  5:1059219/150‑1 (MQ=255)
                                                                                                              gagaAAGGGTTATTACGGAAATTAACTTCCTAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATta                                     <  8:1379860/151‑1 (MQ=255)
                                                                                                              gagaAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAAtt                                      <  6:895857/150‑1 (MQ=255)
                                                                                                               agaAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATtat                                    <  5:1484244/151‑1 (MQ=255)
                                                                                                                gaAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATtat                                    >  8:688937/1‑150 (MQ=255)
                                                                                                                  aaGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCATGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATAtt                                 >  7:510648/1‑151 (MQ=255)
                                                                                                                  aaGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATAtt                                 >  6:690476/1‑151 (MQ=255)
                                                                                                                   aGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATAtt                                 >  3:114195/1‑150 (MQ=255)
                                                                                                                    gggTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTaa                               <  2:87548/151‑1 (MQ=255)
                                                                                                                      gTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAgt                             <  5:715924/151‑1 (MQ=255)
                                                                                                                         attaCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAgtgt                           <  5:571724/150‑1 (MQ=255)
                                                                                                                            aCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCAtt                       >  7:1113756/1‑151 (MQ=255)
                                                                                                                            aCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCAt                        <  3:1393064/150‑1 (MQ=255)
                                                                                                                              ggAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTc                      >  2:1494384/1‑150 (MQ=255)
                                                                                                                              ggAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTc                      >  1:459347/1‑150 (MQ=255)
                                                                                                                               gAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCa                     >  4:540902/1‑150 (MQ=255)
                                                                                                                                aaaTTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAat                   >  3:274099/1‑151 (MQ=255)
                                                                                                                                aaaTTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAat                   >  2:1121188/1‑151 (MQ=255)
                                                                                                                                      aCTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGtt             <  7:807476/151‑1 (MQ=255)
                                                                                                                                      aCTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGt              <  3:1536456/150‑1 (MQ=255)
                                                                                                                                       cTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGttt            >  1:1624380/1‑151 (MQ=255)
                                                                                                                                         tCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGtttt           <  5:620932/150‑1 (MQ=255)
                                                                                                                                          ccGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTa         <  3:570284/151‑1 (MQ=255)
                                                                                                                                             aaTATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAgg       >  4:791340/1‑150 (MQ=255)
                                                                                                                                              atatAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCTATATGGTTTTTAgg       >  4:372749/1‑149 (MQ=255)
                                                                                                                                              atatAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAgg       >  6:1495873/1‑149 (MQ=255)
                                                                                                                                              atatAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAg     <  6:399915/151‑1 (MQ=255)
                                                                                                                                               tataAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGt    >  1:521021/1‑151 (MQ=255)
                                                                                                                                                ataAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGt    <  5:1601249/150‑1 (MQ=255)
                                                                                                                                                 taAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGttt  >  5:1329451/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTT  >  NC_000913/2137395‑2137690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: