BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,543	2 bp→TT	coding (236‑237/1365 nt)	ygdH →	UPF0717 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,543	0	C	T	96.4%	1583.7 / 28.5	478	P79L (CCC→CTC)	ygdH	UPF0717 family protein
Reads supporting (aligned to +/- strand): ref base C (7/10); new base T (209/250); total (217/261)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913	2,926,544	0	C	T	96.4%	1584.3 / 29.5	478	P79P (CCC→CCT)	ygdH	UPF0717 family protein
Reads supporting (aligned to +/- strand): ref base C (6/11); new base T (208/250); total (217/261)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 478 total aligned reads displayed.

TATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCA  >  NC_000913/2926398‑2926687

||

tATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCgg                                                                                                                                             >  6:1612948/1‑151 (MQ=255)

tATCAACTGTTTCGCAACTGTTCACATGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCgg                                                                                                                                             >  8:88245/1‑151 (MQ=255)

   cAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAag                                                                                                                                          <  8:306788/151‑1 (MQ=255)

    aaCTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAaga                                                                                                                                         <  4:724574/151‑1 (MQ=255)

    aaCTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAaga                                                                                                                                         <  6:539636/151‑1 (MQ=255)

     aCTGTTTCGGAACTGTTGACTTGCCTTTCTGATCTGCGGTAGTTTGAGCGATAACTGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGaa                                                                                                                                           <  2:53689/148‑1 (MQ=255)

       tGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGc                                                                                                                                      >  1:881851/1‑151 (MQ=255)

       tGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGc                                                                                                                                      >  5:1166238/1‑151 (MQ=255)

        gTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCt                                                                                                                                     >  7:961133/1‑151 (MQ=255)

          ttCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCttt                                                                                                                                   >  8:505584/1‑151 (MQ=255)

            cGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTg                                                                                                                                 <  8:136862/151‑1 (MQ=255)

              cAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTc                                                                                                                               >  7:1662762/1‑151 (MQ=255)

               aaCTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCg                                                                                                                              >  5:1297531/1‑151 (MQ=255)

                   gTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGAt                                                                                                                            <  5:1476562/149‑1 (MQ=255)

                   gTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGAt                                                                                                                            <  5:851737/149‑1 (MQ=255)

                     tCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATggg                                                                                                                         <  2:141075/150‑1 (MQ=255)

                      cACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCg                                                                                                                       >  1:49742/1‑151 (MQ=255)

                      cACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCg                                                                                                                       >  7:408205/1‑151 (MQ=255)

                          tGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAat                                                                                                                    <  8:1686969/150‑1 (MQ=255)

                               tACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                                                                              >  3:354487/1‑151 (MQ=255)

                               tACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                                                                              >  1:1162822/1‑151 (MQ=255)

                                aCTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcgc                                                                                                             >  6:1191073/1‑151 (MQ=255)

                                aCTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGGTTTGAAAATTGCGATATTAACGTCTTGCGCCGGGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGTAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                                                                              <  7:1377820/150‑1 (MQ=255)

                                  tGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCgcgc                                                                                                           >  6:1402763/1‑151 (MQ=255)

                                   gAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcgcg                                                                                                            <  3:53018/149‑1 (MQ=255)

                                   gAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCgcgc                                                                                                           <  8:1625572/150‑1 (MQ=255)

                                      cTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTg                                                                                                       >  1:68660/1‑151 (MQ=255)

                                                ttGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACt                                                                                             >  4:546580/1‑151 (MQ=255)

                                                 tGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACt                                                                                             <  7:1218497/150‑1 (MQ=255)

                                                  gACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACtt                                                                                            <  8:614180/150‑1 (MQ=255)

                                                    ccGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGtt                                                                                         <  2:700397/151‑1 (MQ=255)

                                                     cGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTc                                                                                        >  3:1160462/1‑151 (MQ=255)

                                                      gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                                                                                       >  5:492838/1‑151 (MQ=255)

                                                      gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                                                                                       <  1:1252158/151‑1 (MQ=255)

                                                       aTAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                                                                                      <  5:1067051/151‑1 (MQ=255)

                                                       aTAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                                                                                      >  4:1000619/1‑151 (MQ=255)

                                                         aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgcg                                                                                     <  6:563719/150‑1 (MQ=255)

                                                         aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgcg                                                                                     <  4:1548248/150‑1 (MQ=255)

                                                          aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGt                                                                                   <  8:1219705/151‑1 (MQ=255)

                                                          aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGt                                                                                   <  2:1058398/151‑1 (MQ=255)

                                                           cAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTc                                                                                  >  2:322822/1‑151 (MQ=255)

                                                             gCAAATAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCAGTGAACGAGGCTTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGACAACTTGTTCGCTGGCCt                                                                                <  1:604164/151‑1 (MQ=255)

                                                               aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCATGc                                                                              >  7:1129239/1‑151 (MQ=255)

                                                                aaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCg                                                                             <  5:744021/151‑1 (MQ=255)

                                                                  gAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTg                                                                           <  4:891543/151‑1 (MQ=255)

                                                                   aaTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTg                                                                           >  6:200895/1‑150 (MQ=255)

                                                                       gCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAt                                                                       <  1:1050237/150‑1 (MQ=255)

                                                                         tGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtct                                                                    >  8:741661/1‑151 (MQ=255)

                                                                          gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtctc                                                                   <  3:1373115/151‑1 (MQ=255)

                                                                            ctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 >  6:317508/1‑151 (MQ=255)

                                                                            ctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 >  7:844534/1‑151 (MQ=255)

                                                                              cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTctct                                                                  <  7:1527084/148‑1 (MQ=255)

                                                                              cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 <  3:1426545/149‑1 (MQ=255)

                                                                              cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 <  2:1136052/149‑1 (MQ=255)

                                                                              cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 <  2:1224062/149‑1 (MQ=255)

                                                                              cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCtt                                                                 <  7:759296/149‑1 (MQ=255)

                                                                               gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGt                                                              <  2:1436797/151‑1 (MQ=255)

                                                                                ttttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGtt                                                             >  5:9694/1‑151 (MQ=255)

                                                                                 tttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTTTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGtt                                                             <  1:1478847/150‑1 (MQ=255)

                                                                                 tttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCTCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGttt                                                            >  2:170614/1‑151 (MQ=255)

                                                                                   tGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTAc                                                          >  6:647585/1‑151 (MQ=255)

                                                                                    gAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTAc                                                          <  1:1392969/150‑1 (MQ=255)

                                                                                     aaaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACgg                                                        <  6:752262/151‑1 (MQ=255)

                                                                                       aaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGc                                                      >  8:818891/1‑151 (MQ=255)

                                                                                       aaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGc                                                      <  6:1293003/151‑1 (MQ=255)

                                                                                         tttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCa                                                     >  6:994292/1‑150 (MQ=255)

                                                                                          ttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCaa                                                    <  1:270256/150‑1 (MQ=255)

                                                                                          ttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCaa                                                    >  5:264404/1‑150 (MQ=255)

                                                                                          ttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAAATATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCaa                                                    <  3:850421/150‑1 (MQ=255)

                                                                                                atTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCAt                                              <  5:1617333/150‑1 (MQ=255)

                                                                                                atTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCAt                                              >  3:1495225/1‑150 (MQ=255)

                                                                                                atTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATa                                             <  7:616739/151‑1 (MQ=255)

                                                                                                 ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATa                                             >  1:1220708/1‑150 (MQ=255)

                                                                                                  tAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAac                                           >  6:82196/1‑151 (MQ=255)

                                                                                                  tAACGTCGTGCGCCGTGACCGCGGAGTAAAGCGGGAACTGCTTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCAt                                              <  4:341718/148‑1 (MQ=255)

                                                                                                   aaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAaca                                          <  8:1502308/151‑1 (MQ=255)

                                                                                                    aCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGGCATTCTCTTCGTTTACGGGCAAATCCATAacac                                         >  3:71672/1‑151 (MQ=255)

                                                                                                     cGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGACGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACAcc                                        <  6:688474/151‑1 (MQ=255)

                                                                                                     cGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACAcc                                        >  2:915708/1‑151 (MQ=255)

                                                                                                     cGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACAcc                                        >  6:1452357/1‑151 (MQ=255)

                                                                                                      gTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcg                                       >  7:462918/1‑151 (MQ=255)

                                                                                                       tCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcgt                                      >  3:903391/1‑151 (MQ=255)

                                                                                                          tGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTACTGCTTGACATTCTCTTCGTTTACGGTCAAATCCATAACACcgttcg                                   >  4:1149434/1‑151 (MQ=255)

                                                                                                          tGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCAAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcgtt                                     <  3:277881/149‑1 (MQ=255)

                                                                                                           gcgcCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcgttcgt                                  <  2:453374/151‑1 (MQ=255)

                                                                                                            cgcCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACcgttcgtt                                 >  4:200853/1‑151 (MQ=255)

                                                                                                              ccGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGtttt                               <  2:1502050/151‑1 (MQ=255)

                                                                                                               cGTGAACGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTc                              >  8:1100773/1‑151 (MQ=255)

                                                                                                                   aaCGCGGCGTAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCa                           >  1:806657/1‑150 (MQ=255)

                                                                                                                            tAAAGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAAtct                 >  1:1587086/1‑151 (MQ=255)

                                                                                                                              aaGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCg               <  8:1324833/151‑1 (MQ=255)

                                                                                                                              aaGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCg               >  2:236841/1‑151 (MQ=255)

                                                                                                                               aGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCg               >  5:596893/1‑150 (MQ=255)

                                                                                                                               aGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGa              <  1:412689/151‑1 (MQ=255)

                                                                                                                               aGCTGGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGa              <  2:785789/151‑1 (MQ=255)

                                                                                                                                     aaCTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTc        <  4:971913/151‑1 (MQ=255)

                                                                                                                                      aCTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTcc       <  3:1299664/151‑1 (MQ=255)

                                                                                                                                      aCTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTcc       <  8:1190671/151‑1 (MQ=255)

                                                                                                                                         gATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCg      <  2:9913/149‑1 (MQ=255)

                                                                                                                                          aTTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTcc   >  4:129427/1‑151 (MQ=255)

||

TATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCA  >  NC_000913/2926398‑2926687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`