Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913423,5520TG26.5% 584.6 / 84.2 322G345G (GGT→GGG) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (172/64);  new base G (0/85);  total (173/149)
Fisher's exact test for biased strand distribution p-value = 5.56e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTGG  >  NC_000913/423403‑423701
                                                                                                                                                     |                                                                                                                                                      
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                                       <  4:1373283/151‑1 (MQ=255)
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGAGGGTg                                                                                                                                                       <  4:808833/151‑1 (MQ=255)
gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGGATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                                       >  6:691056/1‑151 (MQ=255)
  cAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                                                     >  5:546972/1‑151 (MQ=255)
   aGCTTGCCGATGCTGTATTATCAGTCGTAATGTCTGGTGAATTAAATTTATCGCGGGGAACACAGTTTTGTCCGCCACTGGCTGAAAGCGCGGTGTAATATGGACGGCTGGGGGCTGTATGTGGTGCATATGCTGGGGGCGGCGGGTgggg                                                                                                                                                    <  6:1427745/151‑1 (MQ=255)
     cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcg                                                                                                                                                  >  4:517352/1‑151 (MQ=255)
      ttGCCGAAGCTGGATTATCAGTCGGAAAGTCAGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgc                                                                                                                                                 >  5:944238/1‑151 (MQ=255)
         acGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGAGGGGAAGACAGTATTGTCAGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGTGGGAGGCGGGTGGGgcgcgc                                                                                                                                               >  1:102646/2‑150 (MQ=255)
          cGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCa                                                                                                                                              >  3:1426994/1‑150 (MQ=255)
            aaGCTGTATTATCTGGCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaata                                                                                                                                           <  1:1227501/151‑1 (MQ=255)
            aaGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                                                                            >  8:541272/1‑150 (MQ=255)
            aaGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                                                                            >  3:15817/1‑150 (MQ=255)
            aaGCTGGATTATCAGGCGGAAAGGCTGGTCAATGAAATTTATCGCGGGGAAGACAGTATGGGCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGAGGGGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaata                                                                                                                                           <  4:152128/151‑1 (MQ=255)
                tGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAatat                                                                                                                                       >  5:1400289/1‑151 (MQ=255)
                 gtaTTATCATTCGGAAAGTCTGTTGAATTAAATTTATCGCGGGGAATACATTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATg                                                                                                                                      <  8:1169735/149‑1 (MQ=255)
                  gATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgc                                                                                                                                     >  8:163316/1‑151 (MQ=255)
                   aTTATCATTCGTAAAGTGTGGTTATTCAAATTTATCGCTGGCAACACATTATTGTCCGCCACTGGCTGAAAGCGCGGTGTAATTTGGCGGGCGGGCGGCTGGATGTGGTGCATATGCTGGGGGCGGGGGGTGGGGCGCGCAATAATATgc                                                                                                                                     <  6:259853/150‑1 (MQ=255)
                      aTCAGTCGGAAAGTGTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGGCCGCCACTGGCTGAAAGCGCCGGGGAATATGGACGGCTGGCGGCTGGATGTGGGGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATgcagc                                                                                                                                  <  5:420101/150‑1 (MQ=255)
                      aTCAGTCGGAAAGTCTGGTGATTTAAATTTATCGCGGGGAAGAAAGTTTTGGCCGCCAGTGGGTTAACGGGCGGTGTAATATGGACGGCGGGCGGCTGTATGGGGGGCATATGCGGGGGGCGCGGGGGGGGGCGCGCAATAATATgcagca                                                                                                                                 <  5:28820/151‑1 (MQ=255)
                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagcc                                                                                                                                 >  3:326770/1‑150 (MQ=255)
                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                                                                                  >  3:452569/1‑150 (MQ=255)
                        cAGTCGCAAAGTCTGGTGAATTAAATTTATCGGGGGCAAGACAGTATTGGCCGCCACGGGCTGAACGCGCCGGGTAATAGGGCCGGCTGGCGGGGGGATGGGGGGCATATGCGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACg                                                                                                                               <  2:924395/151‑1 (MQ=255)
                          gTCGCAAAGTCTGGTTAATGAAATTTAGCGGGGGGAAGACCTTATTGCCCGCCACTGGCTCAACGGGCCGTGGAATTGGGCCGGGTGGCGGGTGTATGGGGTGAATAGGGGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGtt                                                                                                                             <  2:597905/151‑1 (MQ=255)
                              aaaagggggtgtaatgaaATTTACCGGGGGCACCACAGTTTGGGCCGCCACGGGGTCAACGCGCGGGGTATTAGGCCGGGCGGGGGGGGGTAGGGGGTCCATAGGGGGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑g                                                                                                                        <  1:1319165/139‑1 (MQ=255)
                                aaGTCTGGTGAATGAATTTTATCGCGGGCACGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGGGGAATATGGCCGGCTGGCGGGTGTATGTGGTGCATATGCTGGGGGCGGCGGGGGCGGCGCGCAATAATATGCAGCACGTTGCC‑ggg                                                                                                                      <  2:418454/151‑1 (MQ=255)
                                aaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑ggg                                                                                                                      >  8:241845/1‑151 (MQ=255)
                                 aGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑ggg                                                                                                                      >  2:292159/1‑150 (MQ=255)
                                 aGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑ggg                                                                                                                      >  1:143804/1‑150 (MQ=255)
                                     tGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGGT‑GGGGCCGCGGGTGGGGCGCGCAAATAAATTCAGCCCGGTTCC‑GGGATcccc                                                                                                                >  3:605083/1‑151 (MQ=255)
                                      ggTGAATGAAATTACTCGCGGGGACGACCGTAGTGTCCGCCACTGGCTTAAAGCGCCGTGGAATAGGGACGGCGGGCGGCTGGATGTGGTGAATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCAcc                                                                                                                <  8:867346/151‑1 (MQ=255)
                                       gTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCg                                                                                                               >  5:1129645/1‑151 (MQ=255)
                                        tgaatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCg                                                                                                               >  1:15208/1‑150 (MQ=255)
                                          aatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGa                                                                                                              >  4:723337/1‑149 (MQ=255)
                                              aaaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGCTGGCGGCTGGATGTGGGGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAgcggc                                                                                                        <  6:1018809/151‑1 (MQ=255)
                                               aaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAgcggcg                                                                                                       >  3:488901/1‑151 (MQ=255)
                                               aaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAgcggcg                                                                                                       >  7:415744/1‑151 (MQ=255)
                                                      cgcgGGGAAGACATTTTTGTCGGCAAGTGGTTGAACGGGCGTTGTATTTTGTCGGGGTGGGGGGTGTTTGTGGTGCTTTTGGTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCgaaagaa                                                                                                 <  4:911904/150‑1 (MQ=255)
                                                      cgcgGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCgaaagaa                                                                                                 >  5:850227/1‑150 (MQ=255)
                                                           ggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGCTCACCGCAGCGGCGAAAGCACCCCac                                                                                           >  8:1437739/1‑150 (MQ=255)
                                                             aaGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGcc                                                                                         >  7:1652304/1‑151 (MQ=255)
                                                              aGACTGTATTGGCCGCCACGGGCTGAACGCGCCGGGTAATATGGAGGGGTGGCGGGTGGATGTGGTGCAGAGGCTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                                                        <  3:866351/151‑1 (MQ=255)
                                                               gACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGGTGGCGGCTGTTTGTGGTGCATATGGTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCgg                                                                                       <  6:167791/151‑1 (MQ=255)
                                                                acattatgttCCGCCACTGGGTGAAAGCGCGGTGGAATTTGGAGGGCTGCCGGGTGCATGTGGTCCATATGCTGGGGCCGGCGGGTGGGGCGCGCAATAATATGGAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCACCCGGa                                                                                      <  7:1170678/142‑1 (MQ=255)
                                                                aCAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCgg                                                                                       >  2:1520631/1‑150 (MQ=255)
                                                                 cAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                      >  8:669712/1‑150 (MQ=255)
                                                                  aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                                      >  6:1306284/1‑149 (MQ=255)
                                                                         cccGCCACTGGCGGAACGCGCCGGGCAATAGGGACGGCGGGCGGCGGGATGTGGTCCATATGCGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCG‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                                                                             <  6:889789/150‑1 (MQ=255)
                                                                            ccccatGGGTGAAAGCGCCGTTTAAAAGGGCGGGGTGGCGGGGGTGTGGGGTCCATAGGCGGGGGGCTGAGGGGGTGGCGCGCAATAATCTGCAGCACGTTGCC‑GGTATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                                                                          <  3:1112314/146‑1 (MQ=255)
                                                                            ccaccgggCTCAACGCGCCGTGCAATATGGACGGCGGGGGGCGGGTGGGGGGGCATATGCGGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                                                                          <  6:1638388/145‑1 (MQ=255)
                                                                             ccACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                                                                         <  2:1352168/151‑1 (MQ=255)
                                                                             ccACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAAGCCAGCCGGAAGCGTATATTGTc                                                                         >  8:1345756/1‑151 (MQ=255)
                                                                              cACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCg                                                                        >  4:16885/1‑151 (MQ=255)
                                                                                 tGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCg                                                                     >  5:160934/1‑151 (MQ=255)
                                                                                  ggCTTAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGATTATGCTGGGGGCGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGa                                                                    <  5:1305958/151‑1 (MQ=255)
                                                                                   gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGCATATTGTCGGCGa                                                                    >  3:1269566/1‑150 (MQ=255)
                                                                                     tGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGCAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                                  >  4:577634/1‑150 (MQ=255)
                                                                                      gAACGCGCGGTTGATTAGGGACGGCGGGGGGGTGGATGTGGTGCATATGCGGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAt                                                                <  4:1059965/151‑1 (MQ=255)
                                                                                       aacgcgcGGTGGAATATGCCGGGCTGGCGGCTGGAGGTGGTGCATATGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAt                                                                <  1:1196220/147‑1 (MQ=255)
                                                                                       aaaGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtt                                                               >  3:162772/1‑151 (MQ=255)
                                                                                        aaGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                              >  7:1229880/1‑151 (MQ=255)
                                                                                            gcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCCGCCGGAAGCGTATATTGTCGGCGAACATTTTggg                                                          >  8:473201/1‑150 (MQ=255)
                                                                                            gcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                                          >  1:1394740/1‑151 (MQ=255)
                                                                                             ccGTGTAATATGGAGGGATGCCGGCTGTAGGTGGTGCATATCCTGGGGGTGGCTGGTGGGGCTCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCg                                                         <  8:1019387/151‑1 (MQ=255)
                                                                                             ccGTGTAATATGGACGGCTGGCGGCTGGATGTGGGGCATATGCGGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCg                                                         <  1:274179/151‑1 (MQ=255)
                                                                                              gttgtaaTTTGCACGGCTGGCGGGTGTATGTGGTGCTTTTGCGGGGGTAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGa                                                        <  4:1136536/146‑1 (MQ=255)
                                                                                               gTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGATGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAACCCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGAt                                                       >  8:1411764/1‑151 (MQ=255)
                                                                                                 ggAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGc                                                     >  7:263407/1‑151 (MQ=255)
                                                                                                 ggAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACCTTGCC‑GGGATCACCGAAGCGGCGAATCAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGc                                                     >  5:1544127/1‑151 (MQ=255)
                                                                                                  gAATTTGGCCGGCTGGCGGCGGGAGGGGGGGCATAGGCGGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                                    <  2:17801/151‑1 (MQ=255)
                                                                                                    atatGGAGGGGGGGCGGCTGGTGGTGGTGCATATGCTGGGGGCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                                                  <  5:209942/151‑1 (MQ=255)
                                                                                                       tGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCCCCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCCCGGc                                                >  1:934411/1‑150 (MQ=255)
                                                                                                         gACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAAt                                             >  8:804011/1‑151 (MQ=255)
                                                                                                          aCGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAACCCCAGCCGGACGCGGATATTGGCGGCGAACAGTTTGGCGATGCACGGCAATg                                            >  6:1361994/1‑151 (MQ=255)
                                                                                                             gctggcggcgggAGGGGGTGCATTGGGTGGGGCCGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGtt                                         <  7:8386/151‑1 (MQ=255)
                                                                                                              ctggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGtt                                         >  1:490377/1‑150 (MQ=255)
                                                                                                               ggtcggctgcAGGGGGTGCTTATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTAc                                       <  7:67960/148‑1 (MQ=255)
                                                                                                                 ggggctggATGTGGTGCATCTGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGGCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAg                                     <  8:1483185/149‑1 (MQ=255)
                                                                                                                 gcggctggATGTGGGGCATAGGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCAACGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAg                                     <  2:738852/151‑1 (MQ=255)
                                                                                                                 gcggcgggAGGTGGGGCATAGGCTGGGGGAGGCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAg                                     <  1:532235/151‑1 (MQ=255)
                                                                                                                  cggctggATGTGGTGCATATGCTGGGGGAGGC‑GGTGGGGGCCGGAATAATATTCCCCCCGTTGCCGGGGAGCCCGGACCCGGGCAACGAACCCCCCCCGGAGGCGTATA‑TGTGCGGGGACATTTTGGCGCTGCACGGCAATGGGTACCggg                                   >  2:1221270/1‑150 (MQ=255)
                                                                                                                   ggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc                                   >  1:767314/1‑151 (MQ=255)
                                                                                                                        gttgtgGGGCAAAGGCGGGGGGCGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGGATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtg                              <  1:1644938/149‑1 (MQ=255)
                                                                                                                           gtggggatttgggtGGGGGGGGGGGGGGGGGCGCGAAATAATATGCAGCTCGTTGGC‑GGTATCACCGAAGCGGCGAAATTAAGCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGGGATGCACGGCAATGGTTACAGGCCGAtgtg                            <  8:1142196/137‑1 (MQ=255)
                                                                                                                            tggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCAGGAAGCGTATATTGTCGGCGAACATTTTGGCGAAGCACGGCAATGGTTAGAGGCCGATGTgg                           >  1:33385/1‑150 (MQ=255)
                                                                                                                             ggtgCTTTTGGTGGGGGGGGGGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGaa                         <  4:647887/151‑1 (MQ=255)
                                                                                                                             ggggCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGaa                         <  3:49743/148‑1 (MQ=255)
                                                                                                                              gtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa                          >  6:1218414/1‑149 (MQ=255)
                                                                                                                                  atatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACACAGCCGGAAGCCTATATTGTCGGCGAACATTTTGTCGATGAAAGGCAATCGTTACAGGCCGATGTGCAAGATgc                    >  7:1436502/1‑151 (MQ=255)
                                                                                                                                   tttGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgc                    <  8:1578451/148‑1 (MQ=255)
                                                                                                                                     ggggggggttggCGGGGGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccg                  <  5:75401/140‑1 (MQ=255)
                                                                                                                                         gggggCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATg             <  4:281351/151‑1 (MQ=255)
                                                                                                                                              ggggggggggggCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTa        <  4:1416880/147‑1 (MQ=255)
                                                                                                                                              cggggggTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCAGCGAAGCGGCGAAATAAACCCAGCCTGAATCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTa        <  7:983929/147‑1 (MQ=255)
                                                                                                                                              cggCGGGGGGCGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCTGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGGTACAGGCCGATGTGGAAGATGCCGCCATGAACTa        <  6:28836/150‑1 (MQ=255)
                                                                                                                                               ggggggTGGGGCGCGCAATAATATGCAGCAGGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGGGATGCACGGCAATGGTTACAGGCCGATGTGGAAGCTGCCGCCATGAACTAt       <  7:1563663/148‑1 (MQ=255)
                                                                                                                                               ggCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTAt       >  4:45704/1‑151 (MQ=255)
                                                                                                                                                gCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATc      >  1:198933/1‑151 (MQ=255)
                                                                                                                                                  ggggggggCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGt    <  2:1647172/151‑1 (MQ=255)
                                                                                                                                                   ggTGGCGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTg   <  6:993796/151‑1 (MQ=255)
                                                                                                                                                    gTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTgg  >  7:532432/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCC‑GGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCATGAACTATCGTGG  >  NC_000913/423403‑423701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑