Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1450TC29.6% 259.9 / 141.7 198intergenic (‑10/+53)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (54/65);  new base C (8/42);  total (89/109)
Fisher's exact test for biased strand distribution p-value = 2.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-03

Only 100 of 239 total aligned reads displayed.

GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817998‑2818295
                                                                                                                                                    |                                                                                                                                                          
 tGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAaatta                                                                                                                                                         >  6:593809/1‑150 (MQ=38)
  gCCTGCGTTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttatt                                                                                                                                                       <  2:862789/151‑1 (MQ=255)
  gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACAGATGCATCGGG‑ACatagaa                                                                                                                                                        >  8:1017574/1‑146 (MQ=255)
   ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttattt                                                                                                                                                      <  3:312278/151‑1 (MQ=255)
    cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAagttagtgt                                                                                                                                                     >  8:1335533/1‑147 (MQ=255)
    cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACACCCTAAGACCCAGCCCTTAGTAGAGGAGTACTCAAGCCAGCTGAGCTACGGATCCAGCGGG‑GAATGTAgttt                                                                                                                                                     >  5:197504/1‑151 (MQ=9)
     atagttaaaTTACTCTACATCTTCCAACCCATCATTCCGGAATATCTCGGACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTa                                                                                                                                                    <  1:893367/144‑1 (MQ=11)
      gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAtttt                                                                                                                                                     <  4:29536/149‑1 (MQ=255)
      gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTAc                                                                                                                                                   <  3:125698/151‑1 (MQ=255)
      gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCTACCAAAGCCCCAACGCATACTAGCCGAGTACTCTAAACAGCTGAGCTAAGGATCAATCGGG‑AAAGATAATTTa                                                                                                                                                    >  4:625789/1‑150 (MQ=9)
      gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAACCAAAGACCCAACCCATAGTAGCCGAGAACTAAATACAGCTGAGATAAGGATGAAACGGT‑AAAATTATATTa                                                                                                                                                    >  4:617426/1‑150 (MQ=9)
      acgttaaaTTCCTCCAGCTATTCCATACCATAATACCTGAATATCTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTActtt                                                                                                                                                     <  3:1014019/143‑1 (MQ=11)
         gTTAATTCCTCGACCTATTCGATACCTTCATTGCTGAAGATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTAc                                                                                                                                                   <  3:1443580/148‑1 (MQ=14)
          ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCAGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTActgc                                                                                                                                                >  8:162184/1‑150 (MQ=255)
           tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTActgc                                                                                                                                                >  6:301584/1‑149 (MQ=255)
           tAACTCCGCTACCTCTTCGATACCATCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGATACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                               <  4:1431108/150‑1 (MQ=255)
           aatttactcTACATCTTCGAAACCGTAATTGCGCAATATCTCGTACGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgca                                                                                                                                               <  2:569563/142‑2 (MQ=11)
            tctcttccgatatctTCCATACCTTCATTGCTGAAGATGTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTActgcag                                                                                                                                              <  2:118898/138‑3 (MQ=11)
            aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa                                                                                                                                             <  1:1689001/151‑1 (MQ=255)
             aTTCCGCGACCTATTCCATACCTTCATTGCTGAAGATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                          <  1:65173/151‑1 (MQ=14)
              ttactgtagctctTGGATACCTTCATTCATGCAGATTTCGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                          <  5:245382/141‑1 (MQ=11)
               tctgCTATCTCTTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                         <  7:1079678/147‑1 (MQ=255)
                ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                        <  5:1118226/150‑1 (MQ=255)
                ccGCTACCTCTTCCATACCCTCATACAGGAATATCAAGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttt                                                                                                                                       <  7:815020/151‑1 (MQ=11)
                 cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGA‑‑ttttt                                                                                                                                      >  5:1449612/1‑150 (MQ=255)
                  gCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGa‑‑ta                                                                                                                                         >  2:1076388/1‑147 (MQ=9)
                  gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTACTTTActgcaacatatg                                                                                                                                        >  5:421590/1‑142 (MQ=255)
                  gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGa‑‑taata                                                                                                                                      >  6:1317964/1‑147 (MQ=255)
                   cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                        <  8:354228/147‑1 (MQ=9)
                   cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACGGCTGa‑‑tat                                                                                                                                        >  3:353339/1‑147 (MQ=9)
                   cTATCTCTTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAtttt                                                                                                                                                     <  1:1162280/136‑1 (MQ=9)
                   cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGGATCCGAGGACTCTAGCACTCTGCTCTAGGGATGCCGCGGG‑ATATTGATTTTAGGGCGGA‑‑TATT‑GAt                                                                                                                                   >  3:137711/1‑151 (MQ=14)
                     agctattttttACCTTCATAGATGTATATTTAGTAGTATTTGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTATCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGA‑‑ttttttgat                                                                                                                                  <  7:1513730/141‑6 (MQ=11)
                     aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGTGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑tata                                                                                                                                  >  5:680974/1‑150 (MQ=255)
                      ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAGTTTACTGCAGA‑‑tttttttaa                                                                                                                                  >  8:479042/1‑145 (MQ=14)
                      actctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAcc                                                                                                                                <  6:1482249/150‑1 (MQ=255)
                         ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTActgcag                                                                                                                                              >  7:118845/1‑135 (MQ=255)
                         ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAATTTACTGCAGA‑‑ttttttgataccg                                                                                                                              >  5:1559912/1‑142 (MQ=11)
                          ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑TATT‑tatatacct                                                                                                                             >  6:456707/1‑145 (MQ=11)
                            cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATATCGctact                                                                                                                          >  2:553769/1‑151 (MQ=255)
                              aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTaa                                                                                                                        <  3:1254193/151‑1 (MQ=18)
                              aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTaa                                                                                                                        <  2:1407697/151‑1 (MQ=255)
                              aTACCTTAATTTCTCAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGctaac                                                                                                                          <  4:1515555/151‑3 (MQ=14)
                               tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑tttttagatacctataaa                                                                                                                         >  1:1267036/1‑136 (MQ=14)
                                aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAg                                                                                                                      <  3:614452/151‑1 (MQ=255)
                                   ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATATATTTAACAGCTGA‑‑AATT‑GAAAACGCTAATAAAAcc                                                                                                                    >  6:239322/1‑150 (MQ=11)
                                    tCATTTCTGAATATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAg                                                                                                                      <  7:970972/149‑1 (MQ=11)
                                     catagatGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGcc                                                                                                                    <  6:1267015/144‑1 (MQ=11)
                                      aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCatat                                                                                                                <  2:479696/151‑1 (MQ=255)
                                          gtgctcttcagctctaaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGCCGTATCa                                                                                                              <  3:359923/136‑1 (MQ=14)
                                          actaaGATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAGCCGTATCa                                                                                                              <  3:934661/148‑1 (MQ=14)
                                            gAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGGTGGGTTCTTAGCCGAGTACTCTATCAAGCTCAGCTACGGATGCATCGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTa                                                                                                          <  2:437880/151‑1 (MQ=11)
                                            gAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAg                                                                                                            <  5:1389305/149‑1 (MQ=25)
                                              aggttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatttattt                                                                                                                                                      >  3:1626924/7‑108 (MQ=2)
                                                aTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTGCGACCGATCGGTTGGTAGCCGATTACTCTATCCAGCTGAGCTCCGGATGCATCGGG‑AAAGTTATTTTGGTGCTGA‑‑TTTT‑GATAGCGCTAGTAAAGcgcggtcaagtatg                                                                                                        >  5:762690/1‑136 (MQ=14)
                                                    cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAACCCATATCAAGTAAGAGATg                                                                                                   <  1:239758/150‑1 (MQ=255)
                                                       agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATTTTACTGCTGA‑‑TATT‑TTTACAGCTACTAAAGCCCGATCAAATatcacatcctcc                                                                                               >  5:914738/1‑140 (MQ=11)
                                                        gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                              <  6:1052496/151‑1 (MQ=39)
                                                            atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                           <  3:999118/150‑1 (MQ=40)
                                                               gtgtttCCGGTAGTTTTCGAACCTCCTACCGCTCGGTTGGTAGCGTTGTATTATATCCTGCTTAGCTAGGTTTGCATGGGT‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGgga                                                                                       <  4:999718/146‑1 (MQ=2)
                                                                   aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAGCGCTAGTAAAGCCATATCAAGTAAGAGATGGTGCagagcgcaggat                                                                                   >  2:264628/1‑140 (MQ=14)
                                                                      cGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCAGCCGGGAGGCtt                                                                                  >  5:835773/1‑149 (MQ=255)
                                                                          aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAcc                                                                            <  3:829959/151‑1 (MQ=255)
                                                                          aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGaagc                                                                            >  2:938471/1‑149 (MQ=255)
                                                                           ggcagcGACCCTCCGACCGCGCGGCTCGTAGCCGAGTACTCTATCCTGCTGTGATACGGATGCATCGGG‑AAATTTATTTTACTCCTGA‑‑TATT‑GATACCGTTACTATAGCCATATCTCGTACGATATGGTGCATCCGGGAGGATTCGAACCt                                                                           <  4:469749/146‑1 (MQ=255)
                                                                           ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACATCTTA‑‑TAAT‑TATACCGCTACTAAAGCCa                                                                                                                   >  7:229279/1‑111 (MQ=14)
                                                                           ggaTTCGAACCTCCGACCGCGGGGGTCGGAGCCGCGTAGTCTATCCAGATGCTGTACGGATCCATCGGG‑AAATTTATTTTACTGCTCA‑‑TATT‑GCTAACCATACTACCGCAATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCt                                                                           <  1:381776/151‑1 (MQ=255)
                                                                               tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACTGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCgc                                                                       >  1:193029/1‑150 (MQ=255)
                                                                               tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGaagcgacg                                                                        >  1:318477/1‑144 (MQ=255)
                                                                               tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑TATAGAGCTACTAAAGCCATATAAATTAAAAGATCGTGAATCAGGGAGAAttatactcgact                                                                        >  8:1410713/1‑140 (MQ=11)
                                                                                 gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatt                                                                                                                                                           >  7:415010/1‑68 (MQ=32)
                                                                                 gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAatt                                                                                                                                                           <  2:406241/68‑1 (MQ=32)
                                                                                 gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                              <  1:286382/81‑1 (MQ=39)
                                                                                  aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAACGCTAATAAAAACATATCAAGTAAGAGATGGTGCATCAGGCGAGATGCGAAGATCCGag                                                                      >  4:531453/1‑148 (MQ=14)
                                                                                      tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTAGTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACCCTCCGAcccgcgc                                                                >  1:444301/1‑146 (MQ=255)
                                                                                       ccGACCGCTCGGTTCGTAGCCGAGTTTTCTTTTATTATTTGATACTTATTCATCGGT‑TATTTTATTTTACTGCTTA‑‑TATT‑TTTACCTATACTAATGGCATATGATGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                <  4:1427159/150‑1 (MQ=12)
                                                                                        cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAACGCCATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑Gt                                                             <  3:524785/151‑1 (MQ=255)
                                                                                         gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActg                                                                                                                                                 >  2:1528273/1‑70 (MQ=39)
                                                                                         gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATATATTTTACTGCTGA‑‑TATT‑TATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGGGAGGATTAGAAACTCCGACCgaagcga                                                              >  6:480213/1‑144 (MQ=14)
                                                                                            cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGt                                                         <  8:833317/151‑1 (MQ=255)
                                                                                             gCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCAGGGAGGATTAGAACCGCAGAccctctccctcgtc                                                         >  1:740987/1‑139 (MQ=14)
                                                                                                cGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAattta                                                                                                                                                         <  1:3085/55‑1 (MQ=35)
                                                                                                    gcgcaGCCGAGTACTCTATCCAGCTGAGCTACGGATTTTTCTTT‑ATTTTTCTTTTACTCCTGA‑‑TATT‑TATACATATACTAAATCCATTTCAATTAAGTCAGGGTTTATGATGTAGGATTCGACCCTCCGACCGCTCG‑GTTCGTAGCCGAGt                                                 <  4:1110401/147‑1 (MQ=255)
                                                                                                    gcctAGCCGAGGACTCTATCCAGATGTTCTACGGAGGCATCGGC‑AAATGTATATTCCTGGTGC‑‑TCTT‑GTTACCGCTCATTTCTGCAGATCACGTATGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGt                                                 <  1:1628843/148‑1 (MQ=12)
                                                                                                           cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTAt                                          >  3:53383/1‑151 (MQ=255)
                                                                                                           cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCGAGC‑CTCGCGCTCGTCGCCGAGTagacaa                                           >  3:762994/1‑145 (MQ=14)
                                                                                                                      tCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCCGGGAGGATTAGACGCGACGAGCGAAAC‑GATCGTAGCCGAGTAGTCTATCCAGCTGAGc                                >  8:1109085/1‑150 (MQ=17)
                                                                                                                         aGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATAAAGTAAGAGATGGTGAATCCGGGAGGATTCGAAGCGCCGACCGCTCC‑GTTCGTAGCCGAGTACACAATCCAGCTGAGCTACg                            >  1:487290/1‑151 (MQ=21)
                                                                                                                          acTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCAAGCtacctacca                            >  3:1461926/2‑142 (MQ=255)
                                                                                                                                 atcttacgCATCGGG‑AAATTTAATTTCCTCATCA‑‑TCTT‑GCATCCGATAATACAGCAATATCTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAt                     <  3:497070/143‑1 (MQ=2)
                                                                                                                                    ggATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCAGAGCCCACG‑GGTGCTCGCCGTGGACTCACTACAGGTGAGCTACggcgggaggggg                 >  5:544038/1‑141 (MQ=14)
                                                                                                                                       tGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCAGACCGATCG‑GTTCGTAGCCGAGTACTCTATCCAGATGAGCTAcatcggaagagcgtat              >  4:854355/1‑136 (MQ=21)
                                                                                                                                          aTCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGt                                                         <  1:1023905/106‑1 (MQ=14)
                                                                                                                                           tcgggtaaaCTTATTCTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGa                                                   <  6:846174/105‑1 (MQ=17)
                                                                                                                                           attgctacatttatttTACTCTTGA‑‑TATTCTATA‑CGTTCCTTTAGGCATATGTATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact            <  7:366818/142‑1 (MQ=11)
                                                                                                                                            ttgggtttttttttttACTGTTGA‑‑TATT‑GTTTACGTTACTTTATGCATATTAAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactt           <  8:548667/142‑1 (MQ=11)
                                                                                                                                               gtaaaTTTATTTTTATTTTTA‑‑TATT‑GATACTTTTTCTTTAGACTTATCTATTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAacttactt       <  7:1368236/149‑1 (MQ=11)
                                                                                                                                               gaatatttatttTACTGCTTA‑‑TATT‑GATACCGCTACTAAAGCCATATGAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                   <  4:1579186/135‑1 (MQ=255)
                                                                                                                                                  aaTTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgag          >  7:730559/1‑133 (MQ=255)
                                                                                                                                                    tttatttTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTg  >  5:1322123/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                          
GTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTG  >  NC_000913/2817998‑2818295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑