Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1490TC23.0% 301.8 / 105.5 191intergenic (‑14/+49)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (60/67);  new base C (3/35);  total (87/104)
Fisher's exact test for biased strand distribution p-value = 6.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-04

Only 100 of 220 total aligned reads displayed.

GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGC  >  NC_000913/2818000‑2818296
                                                                                                                                                       |                                                                                                                                                     
gCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAa‑tttatt                                                                                                                                                      <  4:91584/151‑1 (MQ=255)
 ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAa‑tttattt                                                                                                                                                     <  7:788269/151‑1 (MQ=255)
  cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACACCCTAAGACCCAGCCCTTAGTAGAGGAGTACTCAAGCCAGCTGAGCTACGGATCCAGCGGG‑GAA‑TGTAgttt                                                                                                                                                    >  5:197504/1‑151 (MQ=9)
  cTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAACCTACGCCCCTGCGGGTGGTAGCCGAGTAGTATCTACAGGTGAGCTCCGGCTTCATCGGG‑AAA‑ATTAtttt                                                                                                                                                    >  5:1557860/1‑151 (MQ=14)
    gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTAtttt                                                                                                                                                    <  4:29536/149‑1 (MQ=255)
    gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTAc                                                                                                                                                  <  3:125698/151‑1 (MQ=255)
    gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCTACCAAAGCCCCAACGCATACTAGCCGAGTACTCTAAACAGCTGAGCTAAGGATCAATCGGG‑AAA‑GATAATTTa                                                                                                                                                   >  4:625789/1‑150 (MQ=9)
    gCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACAACCAAAGACCCAACCCATAGTAGCCGAGAACTAAATACAGCTGAGATAAGGATGAAACGGT‑AAA‑ATTATATTa                                                                                                                                                   >  4:617426/1‑150 (MQ=9)
    catgtaaaTTCCTCGACATCTTGCATACCCTCATTCCTGAATATCACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTActtt                                                                                                                                                    <  2:1164154/143‑1 (MQ=11)
    acggtataTTCCGCATCCTATTCCAAACCCTCCTTGCCGAATATTTCGGATCATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTAc                                                                                                                                                  <  1:436609/144‑1 (MQ=11)
       gTTAATTCCTCGACCTATTCGATACCTTCATTGCTGAAGATTTCGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTAc                                                                                                                                                  <  3:1443580/148‑1 (MQ=14)
        ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCAGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑ATTAATTTActgc                                                                                                                                               >  8:162184/1‑150 (MQ=255)
         tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTActgct                                                                                                                                              <  7:1145445/150‑1 (MQ=255)
         tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTAATTTActgc                                                                                                                                               >  6:301584/1‑149 (MQ=255)
         tAACTCCGCTACCTCTTCGATACCATCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGATACGGATGCATCGGG‑AAA‑TTTATTTTActgct                                                                                                                                              <  4:1431108/150‑1 (MQ=255)
         aatttactcTACATCTTCGAAACCGTAATTGCGCAATATCTCGTACGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTActgca                                                                                                                                              <  2:569563/142‑2 (MQ=11)
          aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑GTTATTTTActgctg                                                                                                                                             >  3:322862/1‑150 (MQ=255)
           aTTCCGCGACCTATTCCATACCTTCATTGCTGAAGATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGa‑‑t                                                                                                                                         <  1:65173/151‑1 (MQ=14)
            ttactgtagctctTGGATACCTTCATTCATGCAGATTTCGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGa‑‑t                                                                                                                                         <  5:245382/141‑1 (MQ=11)
             accGCTAGCTCTTCGATACCTTCATTGCTGAAGATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  5:786409/150‑1 (MQ=255)
              ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  5:1118226/150‑1 (MQ=255)
              ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGGACTCTATCCAGCGGAGCTACTGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  1:308783/150‑1 (MQ=255)
              ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACACCCTCAGAGCGGTAGCTGCCTAGGCGGCTAGTCAATCACTCAGAGGAAGGGCTGGATCGGG‑AAT‑GTTATTTTACTGAAGA‑‑TAtt                                                                                                                                      >  2:721198/1‑151 (MQ=9)
               cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGtttattggaagcgcagacgt                                                                                                                                        >  2:1425297/1‑137 (MQ=11)
               cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTAGTTTACTGCAGA‑‑ttttt                                                                                                                                     >  5:1449612/1‑150 (MQ=255)
                gCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTAATTTACTGCTGa‑‑ta                                                                                                                                        >  2:1076388/1‑147 (MQ=9)
                gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGa                                                                                                                                    <  8:1437819/151‑1 (MQ=255)
                gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑GTTACTTTActgcaacatatg                                                                                                                                       >  5:421590/1‑142 (MQ=255)
                gCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTAATTTACTGCAGa‑‑taata                                                                                                                                     >  6:1317964/1‑147 (MQ=255)
                 cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑tat                                                                                                                                       <  8:354228/147‑1 (MQ=9)
                 cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACGGCTGa‑‑tat                                                                                                                                       >  3:353339/1‑147 (MQ=9)
                 cTATCTCTTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTAtttt                                                                                                                                                    <  1:1162280/136‑1 (MQ=9)
                 cTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGGATCCGAGGACTCTAGCACTCTGCTCTAGGGATGCCGCGGG‑ATA‑TTGATTTTAGGGCGGA‑‑TATTGAt                                                                                                                                   >  3:137711/1‑151 (MQ=14)
                  tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGAt                                                                                                                                   >  1:470572/1‑150 (MQ=255)
                   atctctTCGATACCTTCATTGCTGAAGCTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                  <  1:24159/148‑1 (MQ=16)
                   agctattttttACCTTCATAGATGTATATTTAGTAGTATTTGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTATCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGG‑AAA‑CTTAGTTTACTGCAGA‑‑ttttttgat                                                                                                                                 <  7:1513730/141‑6 (MQ=11)
                   aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGTGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTtata                                                                                                                                  >  5:680974/1‑150 (MQ=255)
                   aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATa                                                                                                                                  <  1:1661868/150‑1 (MQ=255)
                    ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑ATTAGTTTACTGCAGA‑‑tttttttaa                                                                                                                                 >  8:479042/1‑145 (MQ=14)
                    ccTCTTCCATCCCCTCATTGCGCAAGATCACGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGA‑‑ttttttga                                                                                                                                  <  1:906181/149‑5 (MQ=11)
                    catattCCATACGTTAATTTCTGAATATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTActgca                                                                                                                                              <  7:1070937/135‑2 (MQ=11)
                    actctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATAcc                                                                                                                                <  6:1482249/150‑1 (MQ=255)
                        ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑ATTAATTTACTGCAGA‑‑TATTtatatacct                                                                                                                             >  6:456707/1‑145 (MQ=11)
                         tctaTACGTTAATTCATGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGA‑‑ttttttgatacc                                                                                                                              <  8:1274730/145‑9 (MQ=11)
                          cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTTATATCGctact                                                                                                                          >  2:553769/1‑151 (MQ=255)
                           gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGctacta                                                                                                                         <  4:676669/151‑1 (MQ=255)
                            gtACCTTAATTCCTGCAGATGTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                          <  1:812520/150‑1 (MQ=11)
                            atcccTTAATTGCTGAATATTTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                          <  3:647664/148‑1 (MQ=11)
                            aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTaa                                                                                                                        <  3:1254193/151‑1 (MQ=18)
                            aTACCTTAATTTCTGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGctact                                                                                                                          <  3:54107/151‑1 (MQ=11)
                            aTACCTTAATTTCTCAATATTTAGTATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGctaac                                                                                                                          <  4:1515555/151‑3 (MQ=14)
                             tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑GTTAGTTTACTGCAGA‑‑tttttagatacctataaa                                                                                                                        >  1:1267036/1‑136 (MQ=14)
                                 ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TATATTTAACAGCTGA‑‑AATTGAAAACGCTAATAAAAcc                                                                                                                    >  6:239322/1‑150 (MQ=11)
                                  tCATTTCTGAATATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAg                                                                                                                      <  7:970972/149‑1 (MQ=11)
                                   catagatGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGcc                                                                                                                    <  6:1267015/144‑1 (MQ=11)
                                    aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCatat                                                                                                                <  2:479696/151‑1 (MQ=255)
                                        gtgctcttcagctctaaTATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                              <  3:359923/136‑1 (MQ=14)
                                        gtGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAg                                                                                                            <  4:430360/150‑1 (MQ=25)
                                        actaaGATTTAGGATAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑CTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCa                                                                                                              <  3:934661/148‑1 (MQ=14)
                                          gAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGGTGGGTTCTTAGCCGAGTACTCTATCAAGCTCAGCTACGGATGCATCGGT‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTa                                                                                                          <  2:437880/151‑1 (MQ=11)
                                          gAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAg                                                                                                            <  5:1389305/149‑1 (MQ=25)
                                            aggttacGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAa‑tttattt                                                                                                                                                     >  3:1626924/7‑108 (MQ=2)
                                                 tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                    <  5:369353/150‑1 (MQ=40)
                                                   ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTg                                                                                                                                     <  7:1338495/115‑1 (MQ=40)
                                                   ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTg                                                                                                                                     >  2:1334024/1‑115 (MQ=40)
                                                     agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑ATTATTTTACTGCTGA‑‑TATTTTTACAGCTACTAAAGCCCGATCAAATatcacatcctcc                                                                                               >  5:914738/1‑140 (MQ=11)
                                                      gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                              <  6:1052496/151‑1 (MQ=39)
                                                        atagGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGG‑AAA‑TTTATTTTTCTGCTGT‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATc                                                                                            <  5:8479/151‑1 (MQ=255)
                                                          atGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                           <  3:999118/150‑1 (MQ=40)
                                                                   ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑GTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCGAAATCAAGTCAGAGATGTTGAATCCGGGAGGAtt                                                                                  >  4:1221350/1‑150 (MQ=11)
                                                                        aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATAAAGTAAGAGATGTTGAATCCGGGAGGATTAGactc                                                                            >  2:973851/1‑148 (MQ=255)
                                                                         ggaTTCGAACCTCCGACCGCGGGGGTCGGAGCCGCGTAGTCTATCCAGATGCTGTACGGATCCATCGGG‑AAA‑TTTATTTTACTGCTCA‑‑TATTGCTAACCATACTACCGCAATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCt                                                                           <  1:381776/151‑1 (MQ=255)
                                                                             tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACTGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCgc                                                                       >  1:193029/1‑150 (MQ=255)
                                                                             gcGATCCTCCGCCCGCGCGGTTCCTAGCCGAGACCTCCATCCCGATGTTTTTCTTATGAATCGGC‑TAC‑TTTTTTTTACTCCTGA‑‑TATTGTAACCGTTACTACATCCATATCTCGTATCAGATGTTGCATCCGGGAGGATTCGAACCTCCGa                                                                       <  8:1065313/150‑1 (MQ=255)
                                                                               gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTTATACCGCTACTAAAGCCAGATCAAGTAAGAGATGGTGAATCCCGGAGGATTAGACGCTCCGAcc                                                                     >  1:301442/1‑151 (MQ=14)
                                                                                  ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACAGCTACTAAAGCCATATCAAGTAAGAGATCGTGCATAAGGGAGTATTAGACCCGCCGACCGc                                                                   >  2:329516/1‑150 (MQ=14)
                                                                                    tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTAc                                                                                                                                                  <  7:908296/71‑1 (MQ=38)
                                                                                    tCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTAc                                                                                                                                                  >  2:907157/1‑71 (MQ=38)
                                                                                     ccGACCGCTCGGTTCGTAGCCGAGTTTTCTTTTATTATTTGATACTTATTCATCGGT‑TAT‑TTTATTTTACTGCTTA‑‑TATTTTTACCTATACTAATGGCATATGATGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                <  4:1427159/150‑1 (MQ=12)
                                                                                      cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTTATAGCGCTAGTAAAGCCATATCAAGTAAGAGATGTTGAATCAGGGAGGATTAGACCCTCCGAGCggagggg                                                              >  4:942436/1‑145 (MQ=14)
                                                                                      cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAACGCCATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGt                                                              <  3:524785/151‑1 (MQ=255)
                                                                                       gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTActg                                                                                                                                                <  7:1532660/70‑1 (MQ=39)
                                                                                       gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TATATTTTACTGCTGA‑‑TATTTATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGAATCAGGGAGGATTAGAAACTCCGACCgaagcga                                                              >  6:480213/1‑144 (MQ=14)
                                                                                        cccGCTCGGTGCGAAGCCGAGTACTCTATCCTGCTCAGATACGGATGTAGCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGGCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTc                                                            <  3:515188/150‑1 (MQ=18)
                                                                                        aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑ATTAATTTACTGCTGA‑‑TATTGATACCGCTAGAAAAGCCATATAAAGTATCAGATGGTGAATCAGGGAGGATTAGACGCTCCGAgcgcggcgatc                                                            >  7:422719/1‑144 (MQ=11)
                                                                                          cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑ta                                                                                                                                        >  2:260913/1‑73 (MQ=255)
                                                                                          cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑ta                                                                                                                                        <  7:261913/73‑1 (MQ=255)
                                                                                                        ccgtcttctctATCCAGCTCAGGTTCGTAAGGATCGGG‑AAC‑GTTATATTACTGTTGA‑‑TATTGAAACCCGTACTACAGGCATATCTCGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                            <  3:794692/144‑1 (MQ=12)
                                                                                                               ctctATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGa‑‑ttt                                                                                                                                       >  1:1524676/1‑51 (MQ=255)
                                                                                                                  tATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGACGCTCCGACCGGTCGGGTCGTCGCCGAGTGGTCTCTCCCGCTg                                    >  1:232054/1‑149 (MQ=17)
                                                                                                                        acTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCAAGCtacctacca                             >  3:1461926/2‑142 (MQ=255)
                                                                                                                                  ggATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCAGAGCCCACGGGTGCTCGCCGTGGACTCACTACAGGTGAGCTACggcgggaggggg                  >  5:544038/1‑141 (MQ=14)
                                                                                                                                    aTGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCCGTTCGTAGCCGAGTACTCTATACATCTGAACTCCAGATGGAtgaaca                 >  1:605368/1‑145 (MQ=21)
                                                                                                                                       cATCGGGAAAA‑CTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCCAACCACAGCCCCAACGGATACTAGCCGATTAGTCAAACAAGATGAGCTACGGATggcaagggaaa               >  6:818256/1‑140 (MQ=2)
                                                                                                                                        aTCGGGAAAA‑CTTATTCTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGt                                                          <  1:1023905/106‑1 (MQ=14)
                                                                                                                                         tCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcatata            >  4:481953/1‑139 (MQ=255)
                                                                                                                                           ttgggtttttttttttACTGTTGA‑‑TATTGTTTACGTTACTTTATGCATATTAAGTATTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAactt            <  8:548667/142‑1 (MQ=11)
                                                                                                                                             gaata‑tttatttTACTGCTTA‑‑TATTGATACCGCTACTAAAGCCATATGAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                    <  4:1579186/135‑1 (MQ=255)
                                                                                                                                                   tttatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTg   >  5:1322123/1‑151 (MQ=255)
                                                                                                                                                     tatttTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgtg           >  3:296015/1‑129 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAA‑TTTATTTTACTGCTGA‑‑TATTGATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGC  >  NC_000913/2818000‑2818296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑