Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,818,1580TA24.3% 286.5 / 85.5 174intergenic (‑23/+40)argZ/argYtRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (63/68);  new base A (14/28);  total (78/96)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 191 total aligned reads displayed.

TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTT  >  NC_000913/2818009‑2818304
                                                                                                                                                      |                                                                                                                                                      
tAACTCCGCTACCTCTTCGATACCATCATTGCTGAAGATTTCTGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGATACGGATGCATCGGG‑AAATTTATTTTActgct                                                                                                                                                        <  4:1431108/150‑1 (MQ=255)
 actatctcTACATCTTCCATCCCCTCCTATCTGCATATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa                                                                                                                                                      <  2:447681/144‑1 (MQ=11)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                       <  3:352143/150‑1 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTActgctg                                                                                                                                                       >  3:322862/1‑150 (MQ=255)
 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCAACCTCCGCCCCGCCGGGTCGTCGCCGAGTACTGTATCCCCCTGAGCTACGCAGGCATCGCG‑ACAGTTAATTTActggtgt                                                                                                                                                      >  5:1415507/1‑150 (MQ=14)
 aaTTCCGCTACCTCTTCGAAACCTTAATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                       <  8:46989/150‑1 (MQ=255)
  aTTCCGCGACCTATTCCATACCTTCATTGCTGAAGATCTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                                   <  1:65173/151‑1 (MQ=14)
   ttactgtagctctTGGATACCTTCATTCATGCAGATTTCGTACCAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                                   <  5:245382/141‑1 (MQ=11)
   ttCCGCTACATCTTCCAACCCCTCATACCGCAATATGTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGa‑‑t                                                                                                                                                   <  3:1266949/150‑1 (MQ=11)
    tCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTAGAGCAACGAACAGCTCCCTTCGTACCGGAGTTCTCTAGCCAGCTGAGCTACGGCTGCATAGGG‑AAATTTAAAATAATGCTGagtt                                                                                                                                                   >  3:38379/1‑148 (MQ=9)
    accGCTAGCTCTTCGATACCTTCATTGCTGAAGATTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑tat                                                                                                                                                 <  5:786409/150‑1 (MQ=255)
     ccGCTATCTCTTCGATACCTTCATTGCTGAAGGTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TAtt                                                                                                                                                <  7:122024/151‑1 (MQ=25)
     ccGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTACACCCTCAGAGCGGTAGCTGCCTAGGCGGCTAGTCAATCACTCAGAGGAAGGGCTGGATCGGG‑AATGTTATTTTACTGAAGA‑‑TAtt                                                                                                                                                >  2:721198/1‑151 (MQ=9)
       gctagatctTCGAACCCATCATAGCTGAATATGTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑tttttt                                                                                                                                              <  3:356449/145‑3 (MQ=11)
       gCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAATTTACTGCTGa‑‑ta                                                                                                                                                  >  2:1076388/1‑147 (MQ=9)
        cttcctattcattACGTTCCTAGCTCAATATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttt                                                                                                                                               <  3:161314/137‑2 (MQ=11)
         tACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GAt                                                                                                                                            >  1:470572/1‑150 (MQ=255)
          agctattttttACCTTCATAGATGTATATTTAGTAGTATTTGGTGCATCCGGGAGGATTCGAACCTCCGACAGCTCGGTTCGTATCCGAGTACTCTATCCAGCTTAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGA‑‑ttttttgat                                                                                                                                           <  7:1513730/141‑6 (MQ=11)
          agatctTCCATACGATAATTGAGGAATATTTAGTACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgat                                                                                                                                           <  1:302108/148‑6 (MQ=11)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGTGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑tata                                                                                                                                           >  5:680974/1‑150 (MQ=255)
          aCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATa                                                                                                                                           <  1:1661868/150‑1 (MQ=255)
           ccTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTAGTTTACTGCAGA‑‑tttttttat                                                                                                                                           >  8:1507899/1‑145 (MQ=14)
           ccTCTTCCATCCCCTCATTGCGCAAGATCACGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttga                                                                                                                                            <  1:906181/149‑5 (MQ=11)
           actctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAcc                                                                                                                                         <  6:1482249/150‑1 (MQ=255)
            atattgtttACCTTCCTTTCTGCATATTTAGTAGTATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgatac                                                                                                                                         <  4:1450145/143‑8 (MQ=11)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTACTTTACTGCAGA‑‑ttttttgataccg                                                                                                                                       >  6:1236528/1‑142 (MQ=11)
              ctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGAAGA‑‑TAtttagatacct                                                                                                                                       >  8:54144/1‑142 (MQ=11)
                tctaTACGTTAATTCATGAAGATTTCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGA‑‑ttttttgatacc                                                                                                                                        <  8:1274730/145‑9 (MQ=11)
                tCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGc                                                                                                                                       <  1:402301/148‑1 (MQ=255)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATATCGctact                                                                                                                                   >  2:553769/1‑151 (MQ=255)
                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATT‑TATACAGctagt                                                                                                                                   >  5:617510/1‑149 (MQ=255)
                  gATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGctacta                                                                                                                                  <  4:676669/151‑1 (MQ=255)
                   gtACCTTAATTCCTGCAGATGTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGctact                                                                                                                                   <  1:812520/150‑1 (MQ=11)
                   atcccTTAATTGCTGAATATTTAGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGctact                                                                                                                                   <  3:647664/148‑1 (MQ=11)
                   aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTaa                                                                                                                                 <  3:1254193/151‑1 (MQ=18)
                   aTACCTTAATTTCTGAATATTTAGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGctact                                                                                                                                   <  3:54107/151‑1 (MQ=11)
                    tACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCAGA‑‑tttttagatacctataaa                                                                                                                                  >  1:1267036/1‑136 (MQ=14)
                     aCCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCAGA‑‑TAtttttaaacctaaacta                                                                                                                                 >  6:812346/1‑135 (MQ=11)
                        ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATATATTTAACAGCTGA‑‑AATT‑GAAAACGCTAATAAAAcc                                                                                                                             >  6:239322/1‑150 (MQ=11)
                         tCATTTCTGAATATTTCGGACAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAg                                                                                                                               <  7:970972/149‑1 (MQ=11)
                         tCATTCCGGAAGATATCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAACTTACTTTACTGCAGATTTTTT‑GATACCGCTACTAAAg                                                                                                                               <  5:1535590/149‑1 (MQ=11)
                           aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCatat                                                                                                                         <  2:487582/151‑1 (MQ=255)
                               cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGATCCGTTAGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATATTACTGCAGA‑‑TATT‑GTGAGAGCTACAATAGCCacatata                                                                                                                      >  1:190259/1‑147 (MQ=11)
                                 gAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGCACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTa                                                                                                                   <  6:129736/151‑1 (MQ=255)
                                       ttCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAgaga                                                                                                              <  3:982757/150‑1 (MQ=255)
                                        tCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGAt                                                                                                             <  5:369353/150‑1 (MQ=40)
                                         cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAACCCATATCAAGTAAGAGATg                                                                                                            <  1:239758/150‑1 (MQ=255)
                                          ggAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑g                                                                                                                                              <  7:1338495/115‑1 (MQ=40)
                                            ataaTATGGTGCATCGGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCTAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGc                                                                                                        <  6:347640/149‑1 (MQ=255)
                                            agaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTATTTTACTGCTGA‑‑TATT‑TTTACAGCTACTAAAGCCCGATCAAATatcacatcctcc                                                                                                        >  5:914738/1‑140 (MQ=11)
                                             gaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                                       <  6:1052496/151‑1 (MQ=39)
                                               atagGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATAGGG‑AAATTTATTTTTCTGCTGT‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATc                                                                                                     <  5:8479/151‑1 (MQ=255)
                                                    gTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGgga                                                                                                <  1:1156700/151‑1 (MQ=39)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTTctgct                                                                                                                                                        >  5:1327330/1‑92 (MQ=38)
                                                          ccGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCGAAATCAAGTCAGAGATGTTGAATCCGGGAGGAtt                                                                                           >  4:1221350/1‑150 (MQ=11)
                                                             ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAGCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGa                                                                                        >  5:115144/1‑150 (MQ=255)
                                                               aggaTTCGAGCCTCCGACCGCTCGGTTCGTAGCCGAGTACTGTGTCACCCGGCCCTTCTGATGGAGAGTG‑AATAATATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGTGGATTCGCAcc                                                                                     >  5:1109744/1‑151 (MQ=2)
                                                               aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAAcc                                                                                     <  3:829959/151‑1 (MQ=255)
                                                               aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATAAAGTAAGAGATGTTGAATCCGGGAGGATTAGactc                                                                                     >  2:973851/1‑148 (MQ=255)
                                                                ggcagcGACCCTCCGACCGCGCGGCTCGTAGCCGAGTACTCTATCCTGCTGTGATACGGATGCATCGGG‑AAATTTATTTTACTCCTGA‑‑TATT‑GATACCGTTACTATAGCCATATCTCGTACGATATGGTGCATCCGGGAGGATTCGAACCt                                                                                    <  4:469749/146‑1 (MQ=255)
                                                                ggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACATCTTA‑‑TAAT‑TATACCGCTACTAAAGCCa                                                                                                                            >  7:229279/1‑111 (MQ=14)
                                                                ggaTTCGAACCTCCGACCGCGGGGGTCGGAGCCGCGTAGTCTATCCAGATGCTGTACGGATCCATCGGG‑AAATTTATTTTACTGCTCA‑‑TATT‑GCTAACCATACTACCGCAATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCt                                                                                    <  1:381776/151‑1 (MQ=255)
                                                                  aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAAGCTcc                                                                                  >  1:195712/1‑151 (MQ=255)
                                                                    tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATAACGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGaagcgacg                                                                                 >  1:318477/1‑144 (MQ=255)
                                                                    tCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTAGTTTACTGCTGA‑‑TATT‑TATAGAGCTACTAAAGCCATATAAATTAAAAGATCGTGAATCAGGGAGAAttatactcgact                                                                                 >  8:1410713/1‑140 (MQ=11)
                                                                    gcGATCCTCCGCCCGCGCGGTTCCTAGCCGAGACCTCCATCCCGATGTTTTTCTTATGAATCGGC‑TACTTTTTTTTACTCCTGA‑‑TATT‑GTAACCGTTACTACATCCATATCTCGTATCAGATGTTGCATCCGGGAGGATTCGAACCTCCGa                                                                                <  8:1065313/150‑1 (MQ=255)
                                                                      gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                       <  1:286382/81‑1 (MQ=39)
                                                                      gAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTActgctg                                                                                                                                                       >  6:285840/1‑81 (MQ=39)
                                                                       aaCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAACGCTAATAAAAACATATCAAGTAAGAGATGGTGCATCAGGCGAGATGCGAAGATCCGag                                                                               >  4:531453/1‑148 (MQ=14)
                                                                        aCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAACGCTACTAAAGACATATAAAGTAAGAGATCGTGAATCAGGGAGGAttacacgcgactagcgc                                                                            >  2:1391656/1‑136 (MQ=11)
                                                                         ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACAGCTACTAAAGCCATATCAAGTAAGAGATCGTGCATAAGGGAGTATTAGACCCGCCGACCGc                                                                            >  2:329516/1‑150 (MQ=14)
                                                                            ccGACCGCTCGGTTCGTAGCCGAGTTTTCTTTTATTATTTGATACTTATTCATCGGT‑TATTTTATTTTACTGCTTA‑‑TATT‑TTTACCTATACTAATGGCATATGATGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCg                                                                         <  4:1427159/150‑1 (MQ=12)
                                                                            ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAGTTAGTTTACTGCTGA‑‑TATT‑TATACAGCGACTAAAGCCATATAAAGTAAGAGATGTTGCATAAGCGAGTATTAGAAACGCCGACCGcatcg                                                                        >  2:380214/1‑147 (MQ=14)
                                                                             cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑TATAGCGCTAGTAAAGCCATATCAAGTAAGAGATGTTGAATCAGGGAGGATTAGACCCTCCGAGCggagggg                                                                       >  4:942436/1‑145 (MQ=14)
                                                                             cGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAACGCCATATCAAGTAAGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑Gt                                                                      <  3:524785/151‑1 (MQ=255)
                                                                               cccGCTCGGTGCGAAGCCGAGTACTCTATCCTGCTCAGATACGGATGTAGCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGGCATATCAAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTc                                                                    <  3:515188/150‑1 (MQ=18)
                                                                               aCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAAATTAATTTACTGCTGA‑‑TATT‑GATACCGCTAGAAAAGCCATATAAAGTATCAGATGGTGAATCAGGGAGGATTAGACGCTCCGAgcgcggcgatc                                                                     >  7:422719/1‑144 (MQ=11)
                                                                                 cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ta                                                                                                                                                  >  2:260913/1‑73 (MQ=255)
                                                                                 cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGt                                                                  <  8:833317/151‑1 (MQ=255)
                                                                                         gcgcaGCCGAGTACTCTATCCAGCTGAGCTACGGATTTTTCTTT‑ATTTTTCTTTTACTCCTGA‑‑TATT‑TATACATATACTAAATCCATTTCAATTAAGTCAGGGTTTATGATGTAGGATTCGACCCTCCGACCGCTCG‑GTTCGTAGCCGAGt                                                          <  4:1110401/147‑1 (MQ=255)
                                                                                                cGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGACGCTCCGAGC‑CTCGCGCTCGTCGCCGAGTagacaa                                                    >  3:762994/1‑145 (MQ=14)
                                                                                                      ctctATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGa‑‑ttt                                                                                                                                                 >  1:1524676/1‑51 (MQ=255)
                                                                                                               acTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTAGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCAAGCtacctacca                                     >  3:1461926/2‑142 (MQ=255)
                                                                                                                atGAGGTTCGGATGCAGCGGG‑TCATTTATTTTACTGTTGA‑‑TATT‑GATAACGATACTATAGCCATATGAATTATGATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGa                                   <  4:62097/150‑1 (MQ=17)
                                                                                                                          tacgCATCTCG‑AAACTTATTTTATTTCTCA‑‑TATT‑CTAAACGTTAATATAGCCATATCTAGTAATAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                          <  6:470667/147‑1 (MQ=14)
                                                                                                                           aTGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTACGACCGCTCC‑GTTCGTAGCCGAGTACTCTATACATCTGAACTCCAGATGGAtgaaca                         >  1:605368/1‑145 (MQ=21)
                                                                                                                            tGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCAGACCGATCG‑GTTCGTAGCCGAGTACTCTATCCAGATGAGCTAcatcggaagagcgtat                       >  4:854355/1‑136 (MQ=21)
                                                                                                                               aTCGGGAAAACTTATTCTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGt                                                                  <  1:1023905/106‑1 (MQ=14)
                                                                                                                               aTCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcgagtt                    >  5:1630483/1‑140 (MQ=255)
                                                                                                                                tcgggtaaaCTTATTCTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGa                                                            <  6:846174/105‑1 (MQ=17)
                                                                                                                                attgctacatttatttTACTCTTGA‑‑TATTCTATA‑CGTTCCTTTAGGCATATGTATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAact                     <  7:366818/142‑1 (MQ=11)
                                                                                                                                    gaatatttatttTACTGCTTA‑‑TATT‑GATACCGCTACTAAAGCCATATGAATTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                            <  4:1579186/135‑1 (MQ=255)
                                                                                                                                       aaTTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCaaatggcggtgag                   >  7:730559/1‑133 (MQ=255)
                                                                                                                                        atttatttTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAc                                      >  7:955987/1‑125 (MQ=255)
                                                                                                                                          ggtctttCCTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGc          <  4:1553019/147‑1 (MQ=255)
                                                                                                                                             ttttACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTAGTGc          >  5:372680/1‑148 (MQ=255)
                                                                                                                                               ctACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                    <  7:1145349/56‑1 (MQ=18)
                                                                                                                                               ctACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                    <  5:1000310/56‑1 (MQ=18)
                                                                                                                                               ctACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATcc                                                                                                    >  4:1000989/2‑57 (MQ=18)
                                                                                                                                                  ctgctgA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCa                                                                                                       >  2:1219957/1‑51 (MQ=35)
                                                                                                                                                      |                                                                                                                                                      
TAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGG‑AAATTTATTTTACTGCTGA‑‑TATT‑GATACCGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCG‑GTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTT  >  NC_000913/2818009‑2818304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑