Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1630CA23.3% 345.3 / 19.1 234intergenic (+561/+462)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (85/73);  new base A (40/8);  total (151/83)
Fisher's exact test for biased strand distribution p-value = 1.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAC  >  NC_000913/3270028‑3270313
                                                                                                                                       |                                                                                                                                                       
cgaggcGTGGACAGTGATTCATCTTTCTGCCCCTCCAAAAGCCAAATCTCGCGTGTGAGGGTTTTTAAGTCTTGCTGGTTTACGTTACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCaa                                                                                                                                         <  1:89429/146‑1 (MQ=2)
           cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGGATAGCGTGGGCGCAAGTCATTCAAAGGT‑GCCCCCCTCCAAAAACAAAAAcc                                                                                                                              >  1:1509099/1‑151 (MQ=11)
               cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCATTCATATTA‑GAGCGCCTGCAAAAACAAAAACccgcc                                                                                                                          >  8:1569581/1‑151 (MQ=11)
                 atccccctgccgCCCCCCCAAAACCAAAAACCCGGCGTAGGCGGCTTTTACGTAAACCAGGTGAAACTGAGCCATTAGGGGATTTCTTTTGTGTATAGCGGCGTTGTCAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                                        <  5:203545/140‑1 (MQ=2)
                  agttcttactcgcccTCAACAATCATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                                        <  7:199815/138‑1 (MQ=11)
                    atgtttCTGCTACTCGAATATCATAAGACCTCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                                                     <  4:862691/148‑1 (MQ=11)
                    atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGGGTCGTGTCAAGTCCTTCATCGTG‑GTGCGCCGGCAAAAACAAAAACCCGGCGAAgg                                                                                                                     >  6:856323/1‑150 (MQ=11)
                       tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCCTTGATGCTT‑GTGCCCCGGCAAAACCAAAAAACCGCCGCACCggg                                                                                                                  >  5:100040/1‑151 (MQ=11)
                          cTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTAGGGTAAAGCGTGGTGGCAAGTCAATCAAGATA‑AAGCGCCAACAAAAACAATAACCCGCAGAAggacggg                                                                                                                >  6:800129/1‑144 (MQ=2)
                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCGGT‑GGGCGCCTGCAACCGCAAAAACCCGCCGCAGCGGGtgtt                                                                                                              >  1:873916/1‑151 (MQ=11)
                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGAAAGGCAATCAACCTT‑CGTCCCCGCCAAAAGCAAAAACCCGCCGAAGCGCGgttt                                                                                                              >  2:425216/1‑151 (MQ=11)
                           tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCCTCTTGGCAAGTCATTCAAAATA‑ATACACCAACAAAAACAAAAACccgcagcagatcgtttt                                                                                                              >  8:1023897/1‑138 (MQ=2)
                            cccccTCCACAAGCAGAAACCCGCCGGACCGGTTTATTACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                             <  5:960595/150‑1 (MQ=11)
                              cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATAATT‑GTGCACCAACAAAACCAAAAACCCGCCGAAGAGGGTTTTTa                                                                                                            >  7:1299839/1‑150 (MQ=11)
                                 tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGGCATTCATGATT‑GTGCGCCGACAAAAGCAAAAACCCGCCGCAGGGGGGTGTTACGt                                                                                                         >  7:1456530/1‑150 (MQ=11)
                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATGTTT‑GGGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGGGGTTACGGAAAt                                                                                                     >  2:1544912/1‑151 (MQ=11)
                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGGCATTCATGTTT‑GATCACCAACAAAAACAAAAAACCCCCGAAGAGGGGGTTTaacgaaa                                                                                                      >  4:354974/1‑144 (MQ=11)
                                      aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGCTTAAACTGACCGATAAGCCGCTTTCTTTTTTGTATAGCCTCGTGGACATTCATTAATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                                                                                                   <  7:65066/151‑1 (MQ=11)
                                       ccccacaaCCCGCCGAATCTTGTTTTTACGTAAACCCGCTGAAACTCACCGACCCCCCGCCTGCATTTGCGACGAGGGCCGTTGCCAGCCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                                                  <  1:1054978/145‑1 (MQ=2)
                                         cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCACGGAGTGATGGGT‑GTGCGCCGCCAAAACCAAAAACCCGCCGCCGGGGGGTTTTTCGGTAATCAgcg                                                                                                >  2:1363481/1‑149 (MQ=2)
                                          aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGGAGTCCGTCATCTTT‑GTGCCCCGGCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTTAGTTAATCAGGTg                                                                                               >  5:729089/1‑151 (MQ=11)
                                              aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCGAGTCAATCATGGTT‑GGGCCCCGGCAAAACCAAAAACCCGCCGCAGGGGGGGTTTTCGGAAATCACGTGAAAc                                                                                           >  3:1121633/1‑151 (MQ=11)
                                              aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGGTT‑CTGCCCCGCCAAAAGCAAAAACCCGCCGCAGCGGGGTTTTTCGTAAATCAGGTGaaa                                                                                            >  3:669017/1‑150 (MQ=11)
                                              aCCCGCCGAACCGGGTTTTTACGTAAAGCAGGTCACACTGCCCGAGACGCCCCACTCTTTCGGGTACCGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc                                                                                           <  5:1542116/151‑1 (MQ=11)
                                               cccGCCGAAGCGGGTTTTTACGTAAACCAGGTTATTTTTTTATATAATCCGCTTTCTTTTTGTTATAGAGTTTTTGACAGTCTTTTTTCTTT‑CTGGACGTTCTTAAGATAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                                          <  7:998511/151‑1 (MQ=2)
                                                 cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCCTTCATCTTT‑CTGCCCCACCAAAAACAAAAAACCGCCGCACCGCGTTTTTTCCTTAATCAGGGGAAAATg                                                                                         >  5:1212841/1‑150 (MQ=11)
                                                    cgtaacGGTTTTTTACGTAAACCAGGTTAAACTGACCGAAAATCCGCTTTATTTTGGGTATATCCTCGTTGACATTCATCCATCTTT‑CTGCCCCTCCAAAATCAAAAACCCGCCGAACCGGTTTTTTACGTAAATCAGGTGAAACTGACCg                                                                                     <  2:1212184/146‑1 (MQ=255)
                                                    cgcaccGGTTTATTACGTAAATTATTTAAAACTGACCGATAAGCGTCTTTATTTTTTTTATAGAGTCTTTTACAGTCATTGATGTTT‑TTGCGACTAAAAAATCAAAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTGAAACTGACCg                                                                                     <  7:1109012/146‑1 (MQ=255)
                                                    cgcaccGGGTTATTACGTAAATCATGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGGCGTCGAGAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCg                                                                                     <  2:633063/146‑1 (MQ=11)
                                                       ccggtgcctttAGGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTTTATAGATTCGTGGACAGTCATTATTCTTT‑CTTCCCCTCCAAAAGCAAAAAGCCTCGCACGGGTTTTTTTACGTAAATCAGGTGAAACTGACCGATa                                                                                  <  4:882761/143‑1 (MQ=2)
                                                        gCGGGTTTTTACTTAAACCAGGTCAAACGGACCGAGCAGCTGCTCGGGATTGAGTTTAGTTCAGACGTGAGGGCTTCCTATCT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                                                 <  3:222319/151‑1 (MQ=2)
                                                          gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAGAGTCATTCATCGTT‑GGGCCCCAGCAAAAACAAAAACCCGCATAAGAGGGTTGTTACGGAAATGAGGTGaaaattgcccattagc                                                                               >  7:982967/1‑138 (MQ=11)
                                                           ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATCATCTTT‑CGGCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGCCCCATAATcc                                                                              >  1:1324339/1‑151 (MQ=11)
                                                             ccagtaccgaccccAGGGCAAACGCACCGACAACCCCCTTTCTTTTTGTTATAGCGTCGTCGACAGTCAGTCATCATC‑CTGAGTCACCCCACTCCAGACGGGGGCGATTCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                                            <  1:810995/139‑1 (MQ=2)
                                                              ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCAAGATT‑CTGCCCCTCCAAAACCAAAAACCCGCAGAAGCGGGGTTTTACGGAAATAACGTGCAACTTACCCATAAGCCggg                                                                           >  2:838938/1‑151 (MQ=11)
                                                                ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCAAATTT‑GAGCACCTACAAAAACAAAAACCCGCCTCAGCGCGTTTTTACCTAAATGAGGTGAAACTGACCCATAATCCGGGtt                                                                         >  4:1340165/1‑151 (MQ=14)
                                                                 tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTT‑ATGCCCCGGCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCACGTGAAACTTACCGATAAGGCGGGTTc                                                                        >  3:1264982/1‑151 (MQ=14)
                                                                  aCGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTCGAAAGTAATTCATATTT‑GTACCCCCGCAAAAAAAAAAAAAAGCAGAATATGGGTTTTACGTTAATCACGTGAGACTTAGCGATTACCCggctac                                                                        >  1:1459017/1‑146 (MQ=2)
                                                                    gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCCTTCATAATT‑GAGCGCCACCAAAAACAAAAACCCGCCGCAGAAGGTTTGTACGTAAATCACGTGATAGTTCCCCATAATCCGGGTTCTGt                                                                     >  7:1328017/1‑151 (MQ=9)
                                                                     tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCAAAGTT‑ATGCACCAACAAAAACAAAAACCCGCCGCAGCGCGTTTTTACGTAAATCAGGTGAAAATTACCCATAATCCGGGTTCTGtt                                                                    >  6:1157556/1‑150 (MQ=14)
                                                                     tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTT‑CGGCCCCGCCAAAAGCAAAAACCCCCCGCAGCCGGTTTTTACGGAAATCAGGTGAAACATCCCGATAACCCGGGTTCAGtg                                                                    >  2:132095/1‑150 (MQ=14)
                                                                     tAAAACAGGTCAAACTGACCGATAAGGCTCTTTATTTTGTGTATATCGGCGGTGTCCGTCACTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                                                     <  3:857399/150‑1 (MQ=11)
                                                                          cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAATCATAATT‑GAGCACCAACAAAACCAAAAACCCGCCGAAGACGGTTATTAAGAAAATCAGGTGAAACATACCGATAATCCGGGTTCAGtattcg                                                                >  8:450743/1‑145 (MQ=2)
                                                                           aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTT‑GTGCCCCTCCAAAACCAAAAACCCGCCGAAGAGGGGTTTTACGTAAATCAGGTGCAACTGCCCGATAAGCCGGGTTCTGTGGTGGAc                                                              >  2:1417720/1‑151 (MQ=14)
                                                                           aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCATT‑AAGCCCCACCAAAAGCAAAAACCCGCCGACGCGGGTTGTTACGTAAATAACCTGAAACTGACCCATAAGCCGGGGTCTGTGGTGGAc                                                              >  7:1553538/1‑151 (MQ=14)
                                                                           aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTCGAAAGTCAATCAAGTTT‑GAGCCGCAACAAAAACAAAAACCCGCCGAAGAGCGTGTTTAAGGAAATGAGGTGCAAATTACCCATAAACCGGGTTCTGTAGTCGAc                                                              >  8:204058/1‑151 (MQ=9)
                                                                              tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCACGCATTCATGATT‑GGGCCCCCACAAAACCAAAAACCCGCCGCAGCGGGGTTTTAAGTAAATGAGGTGAAAAGGGCCCCTAACCCGGGtggtgtgggggcccgg                                                           >  3:1339748/1‑136 (MQ=2)
                                                                                aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCCGGTTTTTACGTAAATCAGGTGAAACTGAGCCCTAAGCCGGGTTCTGTCGTGGCCAGtc                                                          >  8:853562/1‑150 (MQ=14)
                                                                                 aatttaCCGATTAGCAGCTTTATTTTGTGTATTTGGTAGAGTATATTCATTCATCTTT‑CTGATCCTATAAAATCTTAAACCCGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                                                        <  6:63076/146‑1 (MQ=255)
                                                                                  aCTGACGGCTAAGCCGCTTTCTTTTTCTTATAGGGTCTGGGATAGGCTTGCAGCTTTGCTTCCGAT‑CTAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                                                       <  8:623215/151‑1 (MQ=9)
                                                                                    tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTGATCTTT‑ATGGCCCCCCAAAAACAAAAAACCGCCGCCGCCGGGTTTTAAGTAAAAAACGTGAAAAATACCCATAAGCCGGGTTCTGTGGTGGGCAGgcatta                                                      >  5:34621/1‑149 (MQ=9)
                                                                                    tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCCGGTTATGTCGTGGACAGtcattc                                                      >  5:1328056/1‑150 (MQ=255)
                                                                                     gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCACTGATTCATATTT‑ATTCCCCTCCAAAACCAAAAAACCCCCGCAGCGGGGTTTTACGTTAATAAGGGGTAACTGACCGCTAAGCCGGGTTGTGTCGTGGGCAGtcattcat                                                    >  5:88296/1‑151 (MQ=17)
                                                                                     gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTT‑GTGCCCCACCAAAAGCAAAAACCCCCCGAAGAGGGTTTTTAAGTAAATCAAGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCactgaataat                                                    >  4:1502990/1‑142 (MQ=14)
                                                                                       cccatcaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCTGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                                                  <  2:496132/145‑1 (MQ=255)
                                                                                         gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCACTCAATAATCATT‑AAGCACCGCCAAAAGCAAAAACCCGCCGAATCCGGGTTTTACCTAAATCAGCTGAAACTGAACGAAAACCCGGTGTCTGTCGTCGACAAtcattaaacaac                                                >  1:571997/1‑144 (MQ=9)
                                                                                         ccctaGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGGAAAAACCCTCCGAAGCTGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                                                <  1:1089403/147‑1 (MQ=255)
                                                                                            aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑GTGCCCCTCCAAAAGCAAAAACCCGCCGAAGACGGGTTTTACGTAAAGCAGGTGAAACTGACCGCTAAGCGGGGTTGTGTTGGGGAAAgagttttcgctcggcc                                             >  1:267431/1‑136 (MQ=11)
                                                                                                cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTAAATAATATTT‑AAGACACAACAAAACCAAAAAACCCCCCAATCGCGTAATTAACAAAAAAACGTGAAACAAAACCATAAGCCGGGTTAAGTCGGTGAAAGTCAATAATCTAGGCCaac                                          >  6:382099/1‑148 (MQ=255)
                                                                                                  ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCGGGTTCTGTCGGGGACAGTCATTGATCTAGGCCAGCAAt                                       >  3:387989/1‑151 (MQ=255)
                                                                                                  ctttctttTGGGTATAGCGTCGTGGACAGTCAATGATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCCCCGAATCGGGTTTTTAAGTAAATCAGGTGAAACTGACCCATAACCCGGGTTCTGGGGTGGCCACTCATTCCTCTaccaccccaa                                        >  4:263686/1‑141 (MQ=14)
                                                                                                   tttctttTTTGTTTATAGTCGTGGACATTCATTCATCTTT‑ATTCACATACAAAACCTAAATCCCTACGAATCGGGTTTTTTCTTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                                       <  7:1370040/150‑1 (MQ=16)
                                                                                                   tttctttTGGGTATAGCGTCGTGGTCAGTGATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGGGAAACTGGCCGATAAGCCGGGTTCTGTGGTGGGCAGTCATTCATGTAGGCCAGccagg                                      >  4:1171405/1‑147 (MQ=17)
                                                                                                   gttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGGAAAAACCCCCCGAAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                      <  5:524903/150‑1 (MQ=255)
                                                                                                    ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCTGAAGCGGGTTTTTACTTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                                       <  2:1624294/149‑1 (MQ=255)
                                                                                                    ttctttTGGGTATAGCGTCGTGGGCAGGCATTAATATTT‑GTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTAAGTAAATCAGGTGAAAATGACCGATAAGCCGGGTTATGTGGTGGCCAGTCATTCATCTAGGCCCGCCAt                                       >  4:703275/1‑149 (MQ=17)
                                                                                                    ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAACCTGACCTATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                                      <  2:313201/150‑1 (MQ=255)
                                                                                                        tttttctataGTGGCGTCGACATTTATTTATATTT‑CTGTCTCTCGATAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                  <  8:268490/144‑1 (MQ=18)
                                                                                                        tttGGGTATAGTTTCTTTGAGAGTCATTCATCTTT‑ATGACCCTCAAAAAGAAAAATCCCGACGAAGATGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCt                                   <  7:121891/149‑1 (MQ=18)
                                                                                                        tttGGGTATAGTGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGGAATAACCGGCCGAAGCGGGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGGCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc                                  <  1:831047/150‑1 (MQ=18)
                                                                                                          tGGGTATAGCGTCGTGGACAGTGATTCATCTTT‑GTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTTAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGGGCGTGATTGAGCTAGGCCAGCAATGGCtgccg                               >  8:1064622/1‑147 (MQ=17)
                                                                                                               ataGCGTCGTGGCCAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt                           >  1:231037/1‑150 (MQ=255)
                                                                                                                taGCGTCGTGGACAGTCATTCATCTTT‑CGGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATGAGGTGAAACTGACCGATAAGCCGGGTTCTGTGGTGGGCCGTCATTCATCTAGGCCCGCAATCGCGCACTGGCTc                          >  6:1275102/1‑150 (MQ=18)
                                                                                                                    ttcttGGACATTCATTCATCTTT‑CTGACCCTCCAAAAGCAAAAACCCGCCGAAGCTGTTTTTTACGTAAATCAGTTGAATCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                      <  4:1287040/146‑1 (MQ=25)
                                                                                                                    gtcgtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGCCAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCGCAagcc                     >  7:964210/1‑150 (MQ=255)
                                                                                                                    gtcgtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                      >  3:1508165/1‑150 (MQ=255)
                                                                                                                    gtcgtGGACAGTCATTCATCTTT‑CTGCCCCACCAAAAGCAAACACCGGCCGAAGCGGGTTTTTACGTAACTCAGGTTAACCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                     <  3:1455748/151‑1 (MQ=25)
                                                                                                                     tcgtTGACAGTCATTCATCTTT‑CTGCCCCTCCAATAGCAAAAACCCGCCGAAGCTGGTTTTTACGTAAATCTGGTTAAACTGATCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagca                     <  6:740180/150‑1 (MQ=25)
                                                                                                                      cgtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGAAAGtcatt                                                       >  7:1585883/1‑115 (MQ=255)
                                                                                                                      cgtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcagc                   >  3:1591936/1‑151 (MQ=255)
                                                                                                                       gtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                  >  7:695818/1‑151 (MQ=255)
                                                                                                                       gtGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGcc                  >  1:1190408/1‑151 (MQ=255)
                                                                                                                        gcgACAGGCATGCATCTTT‑CTGCACCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAGCGATAAGCCGGGTACTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCt                 <  3:482997/149‑1 (MQ=25)
                                                                                                                            cAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAccc             >  4:53530/1‑151 (MQ=255)
                                                                                                                             aGTCTTTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGACTCGGTTTTTTTCGTAAATCAGGTGAAACTGACCGATATGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg            <  1:187098/151‑1 (MQ=25)
                                                                                                                             aGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCg            >  1:473973/1‑151 (MQ=255)
                                                                                                                              gTCATTCATCTTT‑CTGCCCCTCCAAAATTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg           <  6:1446928/151‑1 (MQ=255)
                                                                                                                               tcattcatCTTT‑CTGCCCCTCCAAAAGGAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCgg           <  8:303492/150‑1 (MQ=255)
                                                                                                                                cattcatCTTT‑CTGCCCCTCCAAAAGCCACAACCCGCCGAAGCGGGTTTTTACGTAAATCACGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt         <  1:641094/151‑1 (MQ=255)
                                                                                                                                cattcatCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg          <  3:210037/150‑1 (MQ=255)
                                                                                                                                cattcatCTTT‑CTGACTCTCCAAAATGAAAAACCCTCAGAAGAGTGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt         <  6:1405077/151‑1 (MQ=25)
                                                                                                                                   acatCTTT‑CTGCCCCTCCAACAGCAAAACCCCGCCGAAGCGGGTTTTTACGTAAAGCAGGGGAAACTGACCGATAAGCCGGGTTCTGGCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa      <  2:1225754/150‑1 (MQ=255)
                                                                                                                                    catCTTT‑CTGCGACTCCAAAATTAAAAACTTTGAGAATCTGTTTTTTACTTAAATCAGGTGAAACTGTCTGATATTGGGTGTTGTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCa      <  7:36712/150‑1 (MQ=255)
                                                                                                                                    catCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc       >  8:1335379/1‑149 (MQ=255)
                                                                                                                                    catCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc       >  5:1428967/1‑149 (MQ=255)
                                                                                                                                    catCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc       >  6:244696/1‑149 (MQ=255)
                                                                                                                                     atCTTT‑CTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTGGTGTAGAGTCAGTGCGCTAGTCCTGCAATGGTTAGCAGGCTCAACAAGAATACCCCGGTGCAgg    >  2:685905/1‑150 (MQ=2)
                                                                                                                                     atCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAcc              <  5:1263613/141‑1 (MQ=255)
                                                                                                                                     atCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGt    >  6:1658082/1‑151 (MQ=255)
                                                                                                                                       cTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAc  >  3:250948/1‑151 (MQ=255)
                                                                                                                                       |                                                                                                                                                       
AGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTT‑CTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTAC  >  NC_000913/3270028‑3270313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑