Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,192	0	G	C	26.4%	116.7 / 14.6	78	intergenic (‑27/+8)	leuV/leuP	tRNA‑Leu/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (17/36);  new base C (0/19);  total (17/61)
Fisher's exact test for biased strand distribution p-value = 3.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4606042‑4606336                                                                                                                                                       |                                                                                                                                                 aaatcatgtattaaaCTATAAAGGAAGTTAAGATAATTGGGGCGAGGGGTGGTACTTGATACCCCAGGTCGGTAAGGACACTAACTCCTTAAGCTAGCGGGTCTAGCAATTCCGCCAGCTTCGCACAGTCATCTTACTTTTTTTGATATCg                                                                                                                                                  <  7:918943/139‑1 (MQ=255) aaaTCAAGCAAAAAACAAGAAAGCAAGTAAATATAATTGGTGCGCGGGGGGGGACTGCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCg                                                                                                                                                  <  5:1426600/151‑1 (MQ=21)  aaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTCATCTTACTTTTTTTGATATCGc                                                                                                                                                 <  7:325131/151‑1 (MQ=255)   aTCAAGGAAGAAACAAGAAAGGACGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGcc                                                                                                                                                <  3:1238649/151‑1 (MQ=255)     cAAGGAATAAACAAGAATGCAATTAAACAAAATTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                                                                                                                               <  1:593427/150‑1 (MQ=18)     cAAGGAACAAACAAGAAAGGAAGAAAAGATAATTGGGGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                                                                                                                               <  1:1265420/150‑1 (MQ=255)     aaaGGACTAAACACTAAAGGACGTAATGATAATTGGTGCTTGGTGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTc                                                                                                                                              <  8:1596852/150‑1 (MQ=18)     aaaGGAACAAACAACAAAGGAAGTAAACATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                                                                                                                               <  5:305226/149‑1 (MQ=255)      aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                                                                                                                                             <  3:82676/151‑1 (MQ=255)       agtaagaaACAAGAAATCAAGTAATGATATTTGGTGCTAGGGGGGGTACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                                                                                                                               <  8:1086966/145‑1 (MQ=18)         gaagaaACAAGAAAGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGttt                                                                                                                                          <  8:403494/151‑1 (MQ=255)           agaaACAAGAAAGGAATTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTgg                                                                                                                                        <  6:1011686/151‑1 (MQ=255)            aaaaCAAGAAAGGAAGAAAAGAAAATTGGGCCGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                                                                                                                                       <  2:821179/150‑1 (MQ=21)             aaccAAGAACGCAAGTAAACATAATGCGGGCGCGGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                      <  7:1079883/148‑1 (MQ=18)             aaacaacaacgctcgTAATGATATTGGGGGCGTGGGGGGGCACGTTACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                      <  4:311896/137‑1 (MQ=18)             aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                                                                                                                                      <  7:235866/151‑1 (MQ=255)              accaacaaAGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGc                                                                                                                                     <  1:907163/149‑1 (MQ=255)              aaCAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGc                                                                                                                                     <  3:631583/151‑1 (MQ=255)                 aacaaaGGAAGTATTGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAg                                                                                                                                  <  6:231718/148‑1 (MQ=255)                   gaaaccactaaactatatTTGGTGCTCGGGGGGGCACTTCACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCCTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg                                                                                                                                 <  7:830108/136‑1 (MQ=18)                    aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                               <  4:1326775/151‑1 (MQ=255)                    aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                                                                                                                               <  3:588990/151‑1 (MQ=255)                    aaaGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACACAAACGTACGTAAGCAAACTAACAAATGAAGATAGCGCGTCTAGAAATTCCGCCAGCTTCGCAAAGTGATATTAATTTTTTTGATATGGGGGGGGTTGGTGCGAgggg                                                                                                                               >  4:646382/1‑151 (MQ=12)                      aGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg                                                                                                                             <  7:1341257/151‑1 (MQ=255)                      aGGAAGTAAAGATAATTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggg                                                                                                                             <  3:1045178/151‑1 (MQ=255)                       ggAAGTAAACAAACTTGGTGCGAGGGGGTGGACTTCAACCCCCACGTCCGTTAGGACACTAACACCTGAAGCTAGCCCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGAggggggg                                                                                                                            <  6:1683093/151‑1 (MQ=21)                        gaataaaacaaaaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg                                                                                                                           <  3:1038815/140‑1 (MQ=255)                         cagaaattaatatgggTGCGCGGGGGGGGACTTCAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGa                                                                                                                          <  2:800958/137‑1 (MQ=21)                         aattAAAGATAATTGGTGCGTGGGGGGCGACTTGCACCCCCACTTCCTTTAGGACACTAACACCTGAAGCTAGCGAGTCTACCAATTCCGCCACCTTCCCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGa                                                                                                                          <  6:670671/148‑1 (MQ=21)                            tAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCGTCGCACAGTCATCTGACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt                                                                                                                       >  5:43642/1‑151 (MQ=255)                                aacaattggggccttgggggtgACTACAACCCCCACGGCCTTATCGACACTAAAACCTGAAGCTCGCCAGTATACCAATTCCGCCACCATCGCACAGGCATCATACTTTTTTTGATATCCCCACCTTTGGTGCGAGGGGGGGGACTTGAAc                                                                                                                   <  3:975037/136‑1 (MQ=16)                                       ggggCGAGGGTGGGGACTTGAACCCCCACGTCCGTAATGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                                                                                            <  2:1395668/148‑1 (MQ=255)                                       ggTGCGTGGGGGGGGACGTCCACCCCCACGTCCGGAACGACACTAACACCTGCAGCTCGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                                                                                            <  1:1313568/151‑1 (MQ=25)                                        gTGCGAGGGGGGGGACTACAACCCCCACGTCCGTAAGGACAGTAACACCTGACGCTACGGCGTCTACCAAATCCGCCACCTTCCCTCAGTCATCAAACTATTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGt                                                                                                           <  3:241712/151‑1 (MQ=21)                                           gccggggggggCCTTCCACCCCCACGGCCGTAAGGACACTAACACCTGAAGCTACCGCGTCTACCAATTCCGCCACCATCCCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                                                                                                        <  2:1122158/148‑1 (MQ=25)                                           catgggaggtgACTACAACCCGCACGGCCGGAATTACACTAACACCTGATGCTACCCCTTATACCAATTCCGCCACCATCCAACAGGCATCTTACTTTTTTTGAAATCCCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                                                                                                        <  2:164281/148‑1 (MQ=19)                                             agcgtgggtcctacaaCCCGCACGGCCTTAAGGAGACTACATCATGAAGCTACCCCGTAATCTAAATCCGCCACCATCACACAGGCAACATACATTAATTGATGTAGCCTAGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                                                                                                        <  2:1080447/135‑1 (MQ=9)                                              tgttggtgccTTGAACCGGTAGGTCCTTTAGTACAGTAACACCTGTAGTTCGATCGTCTTGGAATTCCGCCAGTTTCGCCCAGTCATCTTAATTTTTTTGATCTCCCCTCTTTTGGGGCG‑TGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                                     <  3:142380/141‑1 (MQ=255)                                                ggttttccTTCAACCCCCCCGGCCGTTATTACACTAAAACCTGATGCTTGCCATTATACAAATTCCGCCACCATCCAACATTCATCTAACTTTTTTTTATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                                                   <  1:1675289/144‑1 (MQ=16)                                                          caagccccACTTCCTTTATTACACTAAAACATGAAGCTAACACTTCTACCAAATCCGCCACCATCCAACACTCATCAAACTTTATTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAaca                                                                                          <  3:1167709/146‑1 (MQ=16)                                                             cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGGGTCGGGTGGTGCGGGGGGGGGGACGTGCACCGCCGCGGCCGATAGCACACGACCCCCTg                                                                                      >  1:872039/1‑151 (MQ=18)                                                              ccccACGGCCGTAAGGACAGTAACACATGAAGCTAGCCCGTATACCAATTCCGCCACCTTCCCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                     <  5:1191752/151‑1 (MQ=37)                                                                ccccttccgatattacagTAAAACCTGAAGATATCGCGTATACAAATTCCGCCACCTACCCACAGGCATCTAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                                                   <  3:442392/137‑1 (MQ=18)                                                                   cGTCCTTAAGGACACTAACACCTTATGCTCGCGCGGAAACCAATTCCGCCACCTTCGAACATGCACCTTACTTTTTTTGATATACCCTTTTTTGGTGCGTGGGGGGGGACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                <  3:1110205/151‑1 (MQ=255)                                                                    ggccGTAATGAAAATAACACCTGAACCTATCGATTATTCCAATTCCGCCACCATCACACATTCAACTTACTTTTTTTGATTTCCCCTCTTTTGGTGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                               <  5:450469/149‑1 (MQ=18)                                                                      ccGAAAGGACACTAACCCCCGAAGCTAGGGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGAAATCCCCTATTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                             <  1:493814/151‑1 (MQ=25)                                                                         ttattacacTAAAACCTGAAGATATGCCTTAAACCAAATCCGGCACCTTCGAACAGGCATCTAACTTTTTTTGATATCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                            <  3:813483/144‑1 (MQ=18)                                                                          atttacacTAACCCCGCACGCTCGCGCGTAAACCAATTCCGCCACGTTGTACCATTCATGTTCGTTTTTTTCTTATCCCCTTTTTTGGGGCGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                                                         <  4:43562/147‑1 (MQ=9)                                                                           aatacacTACCACCTGAAGCTCGACCTGAAACCAAATCCCCCACGATCCCACACTCAACAAACAATTTTTGAACTCCCCTCCTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                        <  6:118407/148‑1 (MQ=9)                                                                            gcacacAAACCCCTCACGCTAGGGCGGGTACGAATTCCGCCACCTACGCCCAGTCTTCTTACTTTTTTTGATTTACCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                       <  1:1619226/149‑1 (MQ=18)                                                                                              attgactggataaaaaTTCCGCCAACTAAACACAGTCAAAATACTTTTTTTGAAAACCCCTCTTTTGGGGGGTGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                      <  5:791178/136‑1 (MQ=14)                                                                                              atccctattaattacaTTCCGCCATCTTACAACATTCATCTTACTTTTTTTGATTTAAACTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                     <  6:702975/136‑1 (MQ=14)                                                                                                   gcttatttataTTCCGGCACCATACAACAGGCAACTAACATTTTTTGATCTCACCTCCTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                                                <  1:378221/141‑1 (MQ=14)                                                                                                       cTACCAATTCCGCCACCTTCGCAGAGTCAGCTTACTTTTTTTGATATCGCCGCGTTTCGTGCGAGTGTGGGGCCTTTCACCCCCACGTAAGAAACAACACGACAACCACACGCTAGCGCGTCAACCACTTCCGGCACCTTCGCCAAGttcc                                            >  5:1490006/1‑148 (MQ=255)                                                                                                            aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcacacgggcaggt                                        >  7:1331122/1‑141 (MQ=255)                                                                                                            aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCCTAAGAACACTAACACCTGAAGCTAGCGCCTCGGCACATTCCGCCACCTTCGCCGAGTGCGAGCa                                        >  3:199169/1‑150 (MQ=25)                                                                                                              atCCGCCACCATACAACAGGCAACATACAATATTTGAAATAGCCTCATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                     <  3:398695/150‑1 (MQ=17)                                                                                                               gccGCCACCAACGCACAGGCACCTAACTTTTTTTGATATCGCCTCGTTTGGGGCGCGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                     <  5:737566/149‑1 (MQ=21)                                                                                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATGGGATCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAATAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGACACCTTCGccaacatgcaggaattt                                    >  4:153504/1‑135 (MQ=18)                                                                                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAAAAACAGAAGATAACGCAAATACAAATTCACCAACATTAGCACACTGAGAGCAatat                                    >  7:1393929/1‑150 (MQ=21)                                                                                                                   ggatgttattacaGTGATCTAACTTTATTTGATTTCTCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAAGACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                <  7:3390/141‑1 (MQ=17)                                                                                                                   gcatctaaaaacaTTCATCTAACTTTTTTTGATCTCCACTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                <  8:779365/141‑1 (MQ=18)                                                                                                                    ccacttcggtccgTCATGTTGCTTTTTTGAATATAACCAAATTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCAATATCAACg                               <  1:303812/139‑1 (MQ=14)                                                                                                                     aggaaacaacatgcatcatACTTTTATTGATTTACCCTCCTTTGGGGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                              <  3:622769/138‑1 (MQ=17)                                                                                                                     accaaaccacaGTCATCAAACTTTATTGGATATCCCCACTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                              <  3:1365032/144‑1 (MQ=18)                                                                                                                     aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCCACCAATATAAACg                               >  5:657829/1‑150 (MQ=255)                                                                                                                     aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACGGAGATCAATATCaa                                 >  1:246522/1‑148 (MQ=18)                                                                                                                         aatatcattCATCTAACTTTTTTTGATTTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                           <  4:263142/142‑1 (MQ=18)                                                                                                                          cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAATATCAACGAGGttt                         >  4:87321/1‑151 (MQ=255)                                                                                                                          cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGATAGCGCCTCACCCAAATCCTCAACAATCGTCAAGACCGAGCAATATCACCGTGGttt                         >  2:1372079/1‑151 (MQ=21)                                                                                                                           aaacaGGCATCTAACTTTTTTTGATATCGCGTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                        <  1:1649784/149‑1 (MQ=255)                                                                                                                             acattgatgatACTTTTTTTGAATTATCGTTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                      <  4:1302552/141‑1 (MQ=17)                                                                                                                             acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCGAGGGACAGCTATATAAACGTAGtggttg                      >  8:19647/1‑146 (MQ=18)                                                                                                                                 gcATCTAACTTTATTTGAATTCCCCTCTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGc                  <  7:827548/150‑1 (MQ=18)                                                                                                                                          attttttGATTTCTCGTCTTTTTGGGCTTGGGGTGGCACTTGAACCGCCACTTCTGTAAGCACACTAACAGCTGAATCTATCTCGTCTTCCTCTTCCTAAACCTTCGCACATTTCTAGCAATATCTACTTGGTTTTTGGTGCGAggggggg         <  6:202124/150‑1 (MQ=255)                                                                                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAGACAGCGAGTTATATAAACGAGTTTGTTCGTGGGAggcgcgtg        >  2:1043956/1‑145 (MQ=14)                                                                                                                                            tttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAAAGACAGATGAATATAAACGAGGTTTTTCGTGCGAgggggtgg        >  1:809252/1‑150 (MQ=17)                                                                                                                                              tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                              <  2:1263270/78‑1 (MQ=255)                                                                                                                                              tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                              >  7:1266853/1‑78 (MQ=255)                                                                                                                                                  atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa  >  5:368341/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                 AAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGA  >  NC_000913/4606042‑4606336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑