Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,006,058 T→C intergenic (‑272/‑204) ygeV ← / → ygeW putative sigma‑54‑interacting transcriptional activator/putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,006,0580TC97.6% 1543.3 / 9.7 425intergenic (‑272/‑204)ygeV/ygeWputative sigma‑54‑interacting transcriptional activator/putative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base T (5/5);  new base C (225/184);  total (236/189)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

ATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACT  >  NC_000913/3005911‑3006205
                                                                                                                                                   |                                                                                                                                                   
    aaTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGATTTCGt                                                                                                                                               >  1:1223472/1‑150 (MQ=255)
    aaTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATATTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGGTTTCGt                                                                                                                                               >  3:771829/1‑150 (MQ=255)
     aTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTAt                                                                                                                                             <  8:410599/151‑1 (MQ=255)
     aTTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTAt                                                                                                                                             >  1:679980/1‑151 (MQ=255)
        aTGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTAtttt                                                                                                                                          >  4:1452823/1‑151 (MQ=255)
         tGGAAGAAATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTAtttt                                                                                                                                          >  8:757413/1‑150 (MQ=255)
          ggAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTac                                                                                                                                        >  7:1283326/1‑151 (MQ=255)
          ggAAGAGATATCACATTTCTATATCAATATCAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTAACTTATTACTGAAACTTAGAAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTac                                                                                                                                        >  7:801531/1‑151 (MQ=255)
            aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTacaa                                                                                                                                      <  1:1033834/151‑1 (MQ=255)
            aaGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTaca                                                                                                                                       >  2:1156282/1‑150 (MQ=255)
             agagATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTacaa                                                                                                                                      >  2:938952/1‑150 (MQ=255)
              gagaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTacaaac                                                                                                                                    <  7:1237306/151‑1 (MQ=255)
                gaTATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAAGGCTTTCGTATTTTacaaaca                                                                                                                                   >  4:129217/1‑150 (MQ=255)
                  tatCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAAcc                                                                                                                                >  5:256330/1‑151 (MQ=255)
                   atCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAAcc                                                                                                                                <  4:178128/150‑1 (MQ=255)
                     cacaTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAAcc                                                                                                                                >  8:1054616/1‑148 (MQ=255)
                     cacaTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAAcc                                                                                                                                >  8:579257/1‑148 (MQ=255)
                      acaTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGaa                                                                                                                            <  3:891225/151‑1 (MQ=255)
                        aTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTacaaaca                                                                                                                                   >  4:462870/1‑142 (MQ=255)
                        aTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTacaaaca                                                                                                                                   <  5:462312/142‑1 (MQ=255)
                        aTTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGc                                                                                                                          >  3:1618639/1‑151 (MQ=255)
                           tCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTg                                                                                                                        <  8:331278/150‑1 (MQ=255)
                           tCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTg                                                                                                                        <  1:351146/150‑1 (MQ=255)
                               tatCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCCATAAAAAAACCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATc                                                                                                                   >  8:1612477/1‑151 (MQ=255)
                                   aaTATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAtt                                                                                                               <  6:715256/151‑1 (MQ=255)
                                    atatGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAAttt                                                                                                              >  5:503066/1‑151 (MQ=255)
                                      atGATAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTtg                                                                                                             <  2:1300432/150‑1 (MQ=255)
                                       tGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTtgtg                                                                                                           >  2:160394/1‑151 (MQ=255)
                                       tGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGAGATAAAAAAAGCCAAACGCTTTAGTATTTTACAAACAACCAGAAGCTGTCATCAATTtgtt                                                                                                           >  2:1428702/1‑150 (MQ=255)
                                        gagaATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTtgtg                                                                                                           <  4:1514666/150‑1 (MQ=255)
                                         agaATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAt                                                                                                         >  6:624468/1‑151 (MQ=255)
                                            aTTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCaa                                                                                                      <  4:1355021/151‑1 (MQ=255)
                                                cggcggTTAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTCATACTTAGCAGCAAATAAATAAATCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTTGCATCAATTTGTGATCAAcccc                                                                                                  <  8:473217/151‑1 (MQ=255)
                                                  gcggTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCcac                                                                                                <  2:1667660/151‑1 (MQ=255)
                                                    ggTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCcacac                                                                                              >  8:1580643/1‑151 (MQ=255)
                                                       gAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCAAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACAtt                                                                                           >  6:638822/1‑151 (MQ=255)
                                                        aGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTa                                                                                          <  7:1357827/151‑1 (MQ=255)
                                                         gTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTAt                                                                                         <  7:161634/151‑1 (MQ=255)
                                                           ttATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATcc                                                                                       >  4:639265/1‑151 (MQ=255)
                                                               cAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACACCACACATTATCCGTc                                                                                    >  5:1076676/1‑150 (MQ=255)
                                                                aaaCTGAAGAGAGATAGCCTGCCCCGTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCa                                                                                   >  1:553828/1‑150 (MQ=255)
                                                                    tGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCAGTCAAAtt                                                                               >  7:331629/1‑150 (MQ=255)
                                                                     gAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTa                                                                              <  8:1321546/150‑1 (MQ=255)
                                                                      aaGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGt                                                                            >  8:722700/1‑151 (MQ=255)
                                                                        gagagaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCt                                                                          <  6:1003954/151‑1 (MQ=255)
                                                                        gagagaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCt                                                                          <  1:841245/151‑1 (MQ=255)
                                                                         agagaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTa                                                                                          >  5:871559/1‑134 (MQ=255)
                                                                         agagaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTa                                                                                          <  4:871973/134‑1 (MQ=255)
                                                                           agaTAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCtttt                                                                       <  1:165729/151‑1 (MQ=255)
                                                                               acccTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAGATTAGTCTTTTGCa                                                                    <  8:1001352/148‑1 (MQ=255)
                                                                               aGCCTGCCCCTTTATCTTATTTCTTATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAg                                                                   <  3:1166472/150‑1 (MQ=255)
                                                                                gCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGc                                                                  >  7:390623/1‑151 (MQ=255)
                                                                                    gCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgc                                                               <  1:73694/150‑1 (MQ=255)
                                                                                    gCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgc                                                               <  5:1360684/150‑1 (MQ=255)
                                                                                     ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgcgc                                                             >  3:656721/1‑151 (MQ=255)
                                                                                     ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTCTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgcgc                                                             >  8:1657283/1‑151 (MQ=255)
                                                                                      cccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCcgcgcg                                                            >  4:1570217/1‑151 (MQ=255)
                                                                                        cTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCgg                                                           >  7:602091/1‑150 (MQ=255)
                                                                                          ttATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGAt                                                         <  2:340803/150‑1 (MQ=255)
                                                                                          ttATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCAGCGCGGAt                                                         >  6:1387041/1‑150 (MQ=255)
                                                                                           tATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATa                                                        >  7:1024377/1‑150 (MQ=255)
                                                                                            aTCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATaa                                                       <  2:562867/150‑1 (MQ=255)
                                                                                                     cTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGcac                                              >  3:281346/1‑150 (MQ=255)
                                                                                                        aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACtt                                          <  2:308572/151‑1 (MQ=255)
                                                                                                          aCTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAt                                        <  5:20822/151‑1 (MQ=255)
                                                                                                             tAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTAttct                                     >  6:196856/1‑149 (MQ=255)
                                                                                                             tAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt                                     >  7:758826/1‑151 (MQ=255)
                                                                                                             tAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt                                     >  8:822021/1‑151 (MQ=255)
                                                                                                               gcagcaAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGtt                                    >  7:1671040/1‑150 (MQ=255)
                                                                                                                   caAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTcc                               <  5:332577/151‑1 (MQ=255)
                                                                                                                   caAATAAATAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTcc                               >  7:73295/1‑151 (MQ=255)
                                                                                                                    aaataaatAACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTccc                              >  8:1207994/1‑151 (MQ=255)
                                                                                                                     aataatttactggATAAAAATAGGCTAACGCTTTCGTATTTTACAAACAACCAGAATCTGGCATCAATTTGTGATCAACCCCACACATTATGCGTCAATTTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTccga                             <  6:1624386/139‑3 (MQ=255)
                                                                                                                     aataaataACGCGATAAAAAAAGCGAAACGCTTTCTTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTTATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCAGACTTATTGTTAGTCCCa                             <  7:1159290/151‑1 (MQ=255)
                                                                                                                     aataaataACGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTccc                              >  5:1454275/1‑150 (MQ=255)
                                                                                                                          ataaCGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGtat                        <  5:798900/151‑1 (MQ=255)
                                                                                                                           taaCGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGtat                        >  4:1149905/1‑150 (MQ=255)
                                                                                                                            aaCGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGtata                       <  3:48070/150‑1 (MQ=255)
                                                                                                                            aaCGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGtata                       <  7:550392/150‑1 (MQ=255)
                                                                                                                            aaCGCGATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTATTGCAGCCGCTCGGATAATTCTGGCACACTTATTGTTAGTCCAAGGtat                        >  1:258263/1‑149 (MQ=255)
                                                                                                                               gcgATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCtg                   >  4:1005640/1‑151 (MQ=255)
                                                                                                                                 gATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCtgtg                 <  1:1294333/151‑1 (MQ=255)
                                                                                                                                 gATAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCtgtg                 <  3:1331773/151‑1 (MQ=255)
                                                                                                                                  aTAAAAAAAGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGa                <  2:759180/151‑1 (MQ=255)
                                                                                                                                    aaaaaaaGCGAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaa              <  6:115517/151‑1 (MQ=255)
                                                                                                                                    aaaaaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaa              <  8:1461343/151‑1 (MQ=255)
                                                                                                                                    aaaaaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaa               >  2:44544/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaaa             <  4:30826/151‑1 (MQ=255)
                                                                                                                                       aaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAaca           <  3:961022/151‑1 (MQ=255)
                                                                                                                                       aaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAaca           <  3:18645/151‑1 (MQ=255)
                                                                                                                                       aaaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGAACAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAaca           <  8:1137698/151‑1 (MQ=255)
                                                                                                                                        aaaGCCAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAaccc          >  6:330109/1‑149 (MQ=255)
                                                                                                                                         aaGCCAAACGCTTTCGTATTTTACAAACACCCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACAcc         >  2:242798/1‑151 (MQ=255)
                                                                                                                                            ccAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAt      >  3:391629/1‑151 (MQ=255)
                                                                                                                                            ccAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAt      >  3:1080241/1‑151 (MQ=255)
                                                                                                                                            ccAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAt      >  2:1043215/1‑151 (MQ=255)
                                                                                                                                             cAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCACCCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAt      >  2:880564/1‑150 (MQ=255)
                                                                                                                                             cAAACGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATc     <  1:1403039/151‑1 (MQ=255)
                                                                                                                                              aaaCGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATc     >  1:640580/1‑150 (MQ=255)
                                                                                                                                              aaaCGCTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATc     >  5:1586683/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGAAATTATGGAAGAGATATCACATTTCTATATCAATATGAGAATTACGGCGGTGAGTTTATCAAACTGAAGAGAGATAGCCTGCCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACT  >  NC_000913/3005911‑3006205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: