Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,187,667 T→A F773Y (TTC→TAC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,6670TA99.5% 1606.9 / ‑3.2 421F773Y (TTC→TAC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (197/221);  total (199/222)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 425 total aligned reads displayed.

ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187519‑4187814
                                                                                                                                                    |                                                                                                                                                   
  gatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAtc                                                                                                                                                 <  7:177653/151‑1 (MQ=255)
   atgGCCGACTCCGGTGCGCGTGGTGCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAtct                                                                                                                                                <  4:1084001/151‑1 (MQ=255)
    tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACAtctc                                                                                                                                               >  1:1537178/1‑151 (MQ=255)
      gCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTcc                                                                                                                                              >  1:390808/1‑150 (MQ=255)
        cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccacc                                                                                                                                           >  3:1289188/1‑151 (MQ=255)
         gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTccaccc                                                                                                                                          <  4:83610/151‑1 (MQ=255)
            tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACg                                                                                                                                       >  8:191568/1‑151 (MQ=255)
              cGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTAATACATCTCCACCCACGGt                                                                                                                                     <  7:245073/151‑1 (MQ=255)
                 tGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCt                                                                                                                                  >  1:904453/1‑151 (MQ=255)
                 tGCGCGTCGTTCTGCCCCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGACGGATGGCTCCATCATCGAAACCCCAATCACCGCGAACTTCCCAGAAGGTCTCACCGTAGAACAGAACAACATCTCCACCCAAGGtcc                                                                                                                                   >  4:1483520/1‑148 (MQ=255)
                  gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCCCGGTGCt                                                                                                                                  <  4:866446/150‑1 (MQ=255)
                    gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGt                                                                                                                               <  3:231003/151‑1 (MQ=255)
                      gTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTa                                                                                                                              >  6:1564039/1‑150 (MQ=255)
                      gTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAACCCGCATGGCACCATCATCGAAACGCCAATCACACCGAACTACAGACAAGGAATGAACCAACTACAGAAATACATCTcaaccaaccgacctccaa                                                                                                                              >  7:1599375/1‑140 (MQ=255)
                        ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTaa                                                                                                                             >  7:1179211/1‑149 (MQ=255)
                        ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAg                                                                                                                           <  2:656700/151‑1 (MQ=255)
                        ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAg                                                                                                                           <  1:1065801/151‑1 (MQ=255)
                         gTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAgg                                                                                                                          >  5:1124337/1‑151 (MQ=255)
                          ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                                         >  3:1478200/1‑151 (MQ=255)
                           tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTc                                                                                                                        >  3:354538/1‑151 (MQ=255)
                            cTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCt                                                                                                                       >  6:107469/1‑151 (MQ=255)
                                  cacaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                                                  >  3:1628330/1‑150 (MQ=255)
                                   acaGATTCGTAAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAt                                                                                                                >  1:799569/1‑151 (MQ=255)
                                    caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATa                                                                                                               <  6:499374/151‑1 (MQ=255)
                                     aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                              >  3:587137/1‑151 (MQ=255)
                                     aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                                              >  7:655047/1‑151 (MQ=255)
                                     aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGCTCTGGCGGATAc                                                                                                              >  4:384255/1‑151 (MQ=255)
                                      gATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                                             <  5:1453959/151‑1 (MQ=255)
                                       aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                                                                                                            <  6:180529/151‑1 (MQ=255)
                                       aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                                                                                                            <  3:1007311/151‑1 (MQ=255)
                                       aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                                                                                                            >  1:1291009/1‑151 (MQ=255)
                                       aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCg                                                                                                            >  3:740913/1‑151 (MQ=255)
                                         tCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGCTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGc                                                                                                           >  3:1506851/1‑150 (MQ=255)
                                            gcAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       <  6:673210/150‑1 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                                                                       >  4:735496/1‑150 (MQ=255)
                                             cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGa                                                                                                      <  4:1427861/151‑1 (MQ=255)
                                                  tGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAAc                                                                                                  <  3:1148458/150‑1 (MQ=255)
                                                   gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACt                                                                                                 <  6:1032073/150‑1 (MQ=255)
                                                    cTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                                                                               >  5:780287/1‑151 (MQ=255)
                                                     tGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                                              >  7:984025/1‑151 (MQ=255)
                                                      ggTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                                                                               <  2:790754/149‑1 (MQ=255)
                                                       gTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGa                                                                                             >  4:1659030/1‑150 (MQ=255)
                                                         aTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACt                                                                                          >  1:495627/1‑151 (MQ=255)
                                                           gCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTcc                                                                                        >  7:1287776/1‑151 (MQ=255)
                                                               ggTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                                                                    <  2:499950/151‑1 (MQ=255)
                                                               ggTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGt                                                                                     <  6:1161522/150‑1 (MQ=255)
                                                                   tGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCt                                                                                >  5:1492558/1‑151 (MQ=255)
                                                                    gATGTCGTAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGATGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCTCTGAAAACTGCGAACTCCGGTTACCTg                                                                               <  8:1362627/151‑1 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACGCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCt                                                                                <  5:634223/150‑1 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTAc                                                                                  <  5:225717/148‑1 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                               <  8:1147641/151‑1 (MQ=255)
                                                                    gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                               >  2:326143/1‑151 (MQ=255)
                                                                     aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                                                                               >  1:289825/1‑150 (MQ=255)
                                                                       ggCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACt                                                                            >  3:409836/1‑151 (MQ=255)
                                                                       ggCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACt                                                                            >  5:722791/1‑151 (MQ=255)
                                                                         cGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcg                                                                          >  6:222073/1‑151 (MQ=255)
                                                                           aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtc                                                                        <  2:909190/151‑1 (MQ=255)
                                                                            aGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGCTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtcgtcg                                                                       >  5:1641917/1‑151 (MQ=255)
                                                                              ccGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                                     <  2:1643118/151‑1 (MQ=255)
                                                                                 gATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTgg                                                                  >  1:625204/1‑151 (MQ=255)
                                                                                   tGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGtt                                                                >  7:725175/1‑151 (MQ=255)
                                                                                    ggCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTg                                                               >  1:1379146/1‑151 (MQ=255)
                                                                                     gCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTg                                                               >  2:257702/1‑150 (MQ=255)
                                                                                      cTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc                                                             >  8:256039/1‑151 (MQ=255)
                                                                                        ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGGCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc                                                             <  7:492836/149‑1 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                                          <  6:287217/151‑1 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                                          >  5:428788/1‑151 (MQ=255)
                                                                                         catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCAGGTTGACGTg                                                          <  2:1057250/151‑1 (MQ=255)
                                                                                          atcatcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGCTTACCTGACTCGTCGTCTGGTTGACGTgg                                                         >  3:461933/1‑151 (MQ=255)
                                                                                               cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc                                                      >  3:960217/1‑149 (MQ=255)
                                                                                                gAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                   <  4:772932/151‑1 (MQ=255)
                                                                                                 aaaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAgg                                                   >  8:638642/1‑150 (MQ=255)
                                                                                                  aaCGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAc                                                 <  7:1052819/151‑1 (MQ=255)
                                                                                                     gCCAATCACCGCGAACTTCCGTGAAGGGCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtg                                              <  6:961583/151‑1 (MQ=255)
                                                                                                      ccAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtgg                                             <  1:268636/151‑1 (MQ=255)
                                                                                                        aaTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCtggtg                                           >  5:1387983/1‑151 (MQ=255)
                                                                                                              cgcgAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTAcc                                     <  8:408207/151‑1 (MQ=255)
                                                                                                                   aCTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGa                                   >  5:507505/1‑148 (MQ=255)
                                                                                                                    cTTCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGCTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc                               >  6:1291309/1‑151 (MQ=255)
                                                                                                                     ttCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAg                                 >  7:871117/1‑148 (MQ=255)
                                                                                                                     ttCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                              >  4:23225/1‑151 (MQ=255)
                                                                                                                      tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGAc                               <  5:1015420/149‑1 (MQ=255)
                                                                                                                      tCCGTGAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACg                              <  2:1185805/150‑1 (MQ=255)
                                                                                                                           gAAGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtgt                         <  6:736636/150‑1 (MQ=255)
                                                                                                                            aaGGTCTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTgg                       >  6:1439966/1‑151 (MQ=255)
                                                                                                                              ggTCTGAACGTACTACAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGt                      >  4:473107/1‑150 (MQ=255)
                                                                                                                                 cTGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAccc                  >  1:199506/1‑151 (MQ=255)
                                                                                                                                  tGAACGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTAccc                  >  8:1573811/1‑150 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa              >  6:859852/1‑150 (MQ=255)
                                                                                                                                      cGTACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGa              >  4:1297371/1‑150 (MQ=255)
                                                                                                                                        tACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg           >  1:735395/1‑151 (MQ=255)
                                                                                                                                        tACTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAgg           >  8:1482394/1‑151 (MQ=255)
                                                                                                                                         aCTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGt          >  8:532510/1‑151 (MQ=255)
                                                                                                                                          cTCCAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGt          >  6:1380593/1‑150 (MQ=255)
                                                                                                                                            ccAGTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTa         <  2:1416709/149‑1 (MQ=255)
                                                                                                                                            ccAGTACTACATCTCCACCCACGCTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTCGTTGACGTCGCGCAGCACCTGGTGGTTACCGAAGACGAATGTGGTACCCATGAAGGTATc       >  4:1186913/1‑151 (MQ=255)
                                                                                                                                              aGTACTACATCTCCACCCACGGTGCTCGAAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCat     <  5:1275781/151‑1 (MQ=255)
                                                                                                                                               gTACTACATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATtg                          <  8:792043/129‑1 (MQ=255)
                                                                                                                                                tactacATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatg  >  7:301/1‑150 (MQ=255)
                                                                                                                                                tactacATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCatg    >  3:1411992/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGAT  >  NC_000913/4187519‑4187814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: