BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,278,051	G→A	Q63* (CAG→TAG)	yejG ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,051	0	G	A	97.7%	1252.2 / 10.2	343	Q63* (CAG→TAG)	yejG	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (5/3); new base A (161/173); total (166/177)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 343 total aligned reads displayed.

GTAGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTGGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGATAGTTCTG  >  NC_000913/2277905‑2278198

 tAGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCtt                                                                                                                                                 >  3:783876/1‑150 (MQ=255)

  aGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCtt                                                                                                                                                 >  2:617549/1‑149 (MQ=255)

  aGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCtt                                                                                                                                                 >  2:440344/1‑149 (MQ=255)

  aGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTa                                                                                                                                                <  3:477137/150‑1 (MQ=255)

    tttGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGtt                                                                                                                                             >  7:59316/1‑151 (MQ=255)

      tGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCATTCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACCGGCACGGCTCCCCTTCACACTCCCGCCCCGAAGATGGCACTTCGATGTCGGTTAACGCCTAGCTTAGttt                                                                                                                                            >  4:1517588/1‑150 (MQ=255)

      tGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGttt                                                                                                                                            <  2:703105/150‑1 (MQ=255)

         aaaGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATAc                                                                                                                                        <  7:383457/151‑1 (MQ=255)

         aaaGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATAc                                                                                                                                        <  5:854465/151‑1 (MQ=255)

          aaGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACa                                                                                                                                       <  2:1346266/151‑1 (MQ=255)

          aaGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACa                                                                                                                                       <  8:182777/151‑1 (MQ=255)

           aGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTGGCTTAGTTTATACAt                                                                                                                                      >  1:450828/1‑151 (MQ=255)

           aGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACAt                                                                                                                                      >  2:118428/1‑151 (MQ=255)

              gTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCAc                                                                                                                                   >  3:1210107/1‑151 (MQ=255)

              gTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCAc                                                                                                                                   <  6:1138203/151‑1 (MQ=255)

                gcGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCg                                                                                                                                 <  4:110032/150‑1 (MQ=255)

                 cGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCg                                                                                                                                 <  5:1670992/150‑1 (MQ=255)

                 cGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCg                                                                                                                                 >  5:613515/1‑150 (MQ=255)

                   gCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGAcc                                                                                                                              >  1:854074/1‑151 (MQ=255)

                   gCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGAcc                                                                                                                              >  5:1199853/1‑151 (MQ=255)

                    cAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGAcc                                                                                                                              >  1:994349/1‑150 (MQ=255)

                      aTTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATg                                                                                                                           >  2:1305082/1‑151 (MQ=255)

                       ttGCCACACCAAAATTTTTCAATCGCCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACACCAGCACCGAACAAGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGCCCATGc                                                                                                                          >  8:454778/1‑151 (MQ=255)

                        tGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGc                                                                                                                          <  4:575705/150‑1 (MQ=255)

                         gCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCa                                                                                                                         <  6:1431498/150‑1 (MQ=255)

                            acacCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTAt                                                                                                                     <  4:801635/151‑1 (MQ=255)

                             cacCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATc                                                                                                                    >  6:648055/1‑151 (MQ=255)

                               ccAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCAc                                                                                                                  <  5:560812/151‑1 (MQ=255)

                                 aaaaTTTTTAAATAGGCATGTTGAAGCAAACTCGTCCTGGAGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACTCCTAGCTTAGTTTATACATCACCGACCATGCATTATCAccc                                                                                                                >  6:762539/1‑150 (MQ=255)

                                  aaaTTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCAt                                                                                                               >  3:1549201/1‑151 (MQ=255)

                                     tttttCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCg                                                                                                            >  1:1579983/1‑151 (MQ=255)

                                       tttCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCggg                                                                                                          >  8:1434849/1‑151 (MQ=255)

                                        ttCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCggg                                                                                                          <  8:908001/150‑1 (MQ=255)

                                         tCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCt                                                                                                        >  3:834034/1‑151 (MQ=255)

                                           aaTCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTaa                                                                                                      <  4:379267/151‑1 (MQ=255)

                                              cGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTTTCACCATCCGGGCTAAGc                                                                                                    >  7:1340769/1‑150 (MQ=255)

                                              cGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGc                                                                                                    <  1:1437253/150‑1 (MQ=255)

                                              cGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGc                                                                                                    >  8:1144276/1‑150 (MQ=255)

                                              cGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCa                                                                                                   <  4:1426867/151‑1 (MQ=255)

                                                 cATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAAtt                                                                                                <  7:801447/151‑1 (MQ=255)

                                                    gTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTaa                                                                                             <  8:1015886/151‑1 (MQ=255)

                                                      tGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAg                                                                                            <  5:783301/150‑1 (MQ=255)

                                                       gcagcaAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCTACCATGCATTATCACCATCCGGGCTAAGCAATTTAAgcg                                                                                          >  6:1590815/1‑151 (MQ=255)

                                                        cagcaAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAgcgc                                                                                         >  3:206219/1‑151 (MQ=255)

                                                           caAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGccac                                                                                      >  3:99145/1‑151 (MQ=255)

                                                            aaaCTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGccacc                                                                                     >  2:1414636/1‑151 (MQ=255)

                                                            aaaCTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGccacc                                                                                     >  6:1284555/1‑151 (MQ=255)

                                                            aaaCTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGccacc                                                                                     >  1:1200678/1‑151 (MQ=255)

                                                               cTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAgg                                                                                  >  5:1461367/1‑151 (MQ=255)

                                                                tCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGc                                                                                 >  4:704977/1‑151 (MQ=255)

                                                                 cGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAgg                                                                                  <  2:992187/149‑1 (MQ=255)

                                                                  gTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCtg                                                                               >  5:960242/1‑151 (MQ=255)

                                                                    ccTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGGTTAGTTTATACATCACCGACCTTGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCtgtt                                                                             <  1:1132667/151‑1 (MQ=255)

                                                                    ccTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCtgt                                                                              <  4:227286/150‑1 (MQ=255)

                                                                        gCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCa                                                                         <  3:673278/151‑1 (MQ=255)

                                                                         cGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCAc                                                                        >  1:61473/1‑151 (MQ=255)

                                                                                 aaaCAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGt                                                                >  2:768582/1‑151 (MQ=255)

                                                                                 aaaCAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGt                                                                >  4:114457/1‑151 (MQ=255)

                                                                                   aCAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAAGTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTcc                                                              >  6:497242/1‑151 (MQ=255)

                                                                                      ggCACCGCTCACCTTCACACTCCCGCACCGAAGATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCTCCACCAGGCTGTTGTCACCGCACGGTCCAtt                                                           <  4:1651637/151‑1 (MQ=255)

                                                                                       gCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCAttt                                                          <  8:1270806/151‑1 (MQ=255)

                                                                                          cGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGc                                                       <  8:829351/151‑1 (MQ=255)

                                                                                          cGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGGTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGc                                                       <  2:298501/151‑1 (MQ=255)

                                                                                            gCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGccg                                                     >  5:342449/1‑149 (MQ=255)

                                                                                                 ccTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCg                                                <  5:736793/151‑1 (MQ=255)

                                                                                                   ttCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTGGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCAcc                                                                       <  6:1031149/126‑1 (MQ=255)

                                                                                                    tCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGt                                              <  6:200432/150‑1 (MQ=255)

                                                                                                     cacaCTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGtt                                             <  7:1558090/150‑1 (MQ=255)

                                                                                                      acacTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCa                                           <  3:965791/151‑1 (MQ=255)

                                                                                                        acTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAAc                                         <  8:566990/151‑1 (MQ=255)

                                                                                                           ccAGCACCGAACATGGAACTTCGATGTCGCTTAACTCCTAGCTTAGTTTATACATCACCGACCATGAATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGTTTCAACCtt                                      <  3:1429532/151‑1 (MQ=255)

                                                                                                            cAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTc                                     <  8:1054066/151‑1 (MQ=255)

                                                                                                            cAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTc                                     <  2:1136428/151‑1 (MQ=255)

                                                                                                            cAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATCCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTc                                     <  7:167914/151‑1 (MQ=255)

                                                                                                             aGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTcc                                    >  6:1106686/1‑150 (MQ=255)

                                                                                                              gCACCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCg                                    >  7:1630815/1‑150 (MQ=255)

                                                                                                                aCCGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAAc                                 >  3:1624409/1‑151 (MQ=255)

                                                                                                                 ccGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATT‑TCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCt                               <  3:1130532/151‑1 (MQ=255)

                                                                                                                  cGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCt                               <  6:372438/151‑1 (MQ=255)

                                                                                                                  cGAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCt                               >  4:132776/1‑151 (MQ=255)

                                                                                                                   gAACATGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTg                              <  8:1498689/151‑1 (MQ=255)

                                                                                                                       aTGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGa                           >  3:436934/1‑150 (MQ=255)

                                                                                                                       aTGGAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGa                           >  1:49653/1‑150 (MQ=255)

                                                                                                                         ggAACTTCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAAc                        >  5:289980/1‑151 (MQ=255)

                                                                                                                               tCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACcagca                   <  7:1315405/150‑1 (MQ=255)

                                                                                                                               tCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACcagca                   <  1:358696/150‑1 (MQ=255)

                                                                                                                               tCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACcagca                   <  1:569390/150‑1 (MQ=255)

                                                                                                                               tCGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACcagc                    >  8:3391/1‑149 (MQ=255)

                                                                                                                                cGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACcagc                    >  2:540533/1‑148 (MQ=255)

                                                                                                                                cGATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGa                 <  8:1375194/151‑1 (MQ=255)

                                                                                                                                 gATGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGAc                >  5:123002/1‑151 (MQ=255)

                                                                                                                                   tGTCGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGAcc               >  4:34535/1‑150 (MQ=255)

                                                                                                                                    ggcGCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCa              <  2:880007/148‑1 (MQ=255)

                                                                                                                                    gTCGCTTAACGCCTAGCGTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAg             <  7:1395293/151‑1 (MQ=255)

                                                                                                                                       gCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCg           >  2:1609659/1‑150 (MQ=255)

                                                                                                                                       gCTTAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGa          <  6:923247/151‑1 (MQ=255)

                                                                                                                                         ttAACGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGAt         >  7:1308945/1‑150 (MQ=255)

                                                                                                                                           aaCGCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGATAGt      >  3:1474263/1‑151 (MQ=255)

                                                                                                                                              gCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGATAGTTCt   <  5:1053361/151‑1 (MQ=255)

                                                                                                                                              gCCTAGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGATAGTTCt   <  1:1008280/151‑1 (MQ=255)

GTAGTTTGAAAAGGGTTCGGCAATTGCCACACCAAAATTTTTCAATCGGCATGTTGCAGCAAACTCGTCCTGGCGATTTACAAACAGGCACGGCTCACCTTCACACTCCAGCACCGAACATGGAACTTCGATGTCGCTTAACGCCTGGCTTAGTTTATACATCACCGACCATGCATTATCACCATCCGGGCTAAGCAATTTAAGCGCCACCAGGCTGTTGTCACCGCACGGTCCATTTTGCGGAATCGGTTCAACCTTCGAACCTGCGAAACCAGCAGACCAGCGATAGTTCTG  >  NC_000913/2277905‑2278198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`