Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4290TG33.4% 535.6 / 131.7 372intergenic (+70/‑89)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (55/190);  new base G (119/4);  total (178/194)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 390 total aligned reads displayed.

GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579
                                                                                                                                                     |                                                                                                                                                      
   tCGCGTGGATTGTTGTGATGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGtcct                                                                                                                                                     <  5:1804193/150‑1 (MQ=255)
          gATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCCTTCTGTAAGCCGGTTAAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTccccc                                                                                                                                             >  1:360769/1‑151 (MQ=255)
           aTTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTcccccc                                                                                                                                            >  7:149964/1‑150 (MQ=255)
             tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                           <  8:621360/150‑1 (MQ=255)
              gttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAat                                                                                                                                         <  1:1726743/151‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAACTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  4:1442502/151‑1 (MQ=255)
                 gtgCTGTTGATTGGCTGGATCTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                      <  2:647648/151‑1 (MQ=255)
                   gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                    <  7:110338/151‑1 (MQ=255)
                   gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCCCCAATATccg                                                                                                                                    >  8:1576042/1‑150 (MQ=255)
                      gtcgATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCa                                                                                                                                 <  7:1729064/148‑1 (MQ=255)
                      gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCGTGCGCCCCCAAATATCCTTc                                                                                                                                  >  7:1824110/1‑150 (MQ=255)
                        tGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAg                                                                                                                                <  1:929155/150‑1 (MQ=255)
                              gCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGAtgatga                                                                                                                         <  6:1728873/151‑1 (MQ=255)
                                 ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGTCAACCTGCCCCAAATATCTTTGAGatgatgcttg                                                                                                                      >  3:1789537/1‑147 (MQ=255)
                                    tGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCGCCGGGCTCGTCGCCCCAATATCAGTCAGAAGCTGAGTGCTc                                                                                                                   >  7:356044/1‑151 (MQ=255)
                                     gTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCTCCCCCAAATATCCTTCAGATGATGAGTGAtac                                                                                                                  >  7:849584/1‑149 (MQ=255)
                                      tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGTTGATGAGTGATccg                                                                                                                 >  3:590209/1‑150 (MQ=255)
                                       ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCt                                                                                                                 <  7:635449/150‑1 (MQ=255)
                                        tAAAAGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCACCACCGCCCCCAAATAACCTTCAGATCATGAGGGATCCTgg                                                                                                               >  8:1401149/1‑150 (MQ=255)
                                         aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCa                                                                                                              <  4:152303/151‑1 (MQ=255)
                                         aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCGTCAGCTGATGAGTGATGCTgg                                                                                                               >  5:1338255/1‑149 (MQ=255)
                                           aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                            <  3:1356667/151‑1 (MQ=255)
                                             gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCCGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGGCGCCCCCAAATATCCTTCAGATGATGAGTTATCCTGCAtt                                                                                                            >  2:1457423/1‑149 (MQ=255)
                                             gccgccACGATCGGCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAg                                                                                                          <  4:100660/151‑1 (MQ=255)
                                              ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTCTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                         <  5:1099269/151‑1 (MQ=255)
                                                 ccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCCGGCGGCGGCCCCCACAGATCCAGCAGATGATGAGTGAGCCTGCAGTTGGcg                                                                                                       >  6:344965/1‑149 (MQ=255)
                                                  cACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCGGCCCCCCAGATCCTTCAGATGATGAGTGATCCTGCATTAgggg                                                                                                       >  6:1603976/1‑147 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCATTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCGCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  7:557090/151‑1 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  1:271326/151‑1 (MQ=255)
                                                   aCGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCAGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                    <  7:1559229/151‑1 (MQ=255)
                                                     gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCGGCCACCCCCAAATATACATCAGATGATGAGTGATGATGGATTAGTCTATgg                                                                                                   >  8:1834695/1‑150 (MQ=255)
                                                     gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                  <  4:1711539/151‑1 (MQ=255)
                                                       tCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCGGCCCCCACAGATCCTGCAGAGGATGAGTGATCCTGCCTTAGGCTATGGcgc                                                                                                >  8:451560/1‑149 (MQ=255)
                                                           cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTTTGGGAAGGTg                                                                                            >  3:748296/1‑151 (MQ=255)
                                                             gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCGCCCCCACAGATCCTGCAGAGGATGAGTGGTGCTGCGTTAGTCGAGGGCAAgggggt                                                                                          >  1:534558/1‑147 (MQ=255)
                                                              ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTGCAGATGATGAGTGATCCTGCATGAGGCTATGGCAAGGTGTTc                                                                                         >  5:1690222/1‑151 (MQ=255)
                                                              ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAGATCCTTCAGATGATGAGTGATCCTGCAGTAGGCTATGGCAAGGTGagg                                                                                         >  3:251080/1‑149 (MQ=255)
                                                                   tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                    >  1:1341918/1‑151 (MQ=255)
                                                                   tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGAGCCTGCATTAGGCTATGGCAAGGTGATCCGAtt                                                                                    >  6:123279/1‑151 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                   <  1:204822/151‑1 (MQ=255)
                                                                    gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                     <  6:817429/149‑1 (MQ=255)
                                                                     aaaaCCAGTACTCCTTGCGTTCTGTCAGCCGGTTCAGGATGATATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                   <  4:1276191/150‑1 (MQ=255)
                                                                     aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                  <  8:1226010/151‑1 (MQ=255)
                                                                     aaaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtttt                                                                                  <  3:1091243/151‑1 (MQ=255)
                                                                      aaaCCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                 <  1:659829/151‑1 (MQ=255)
                                                                        aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                               <  5:1368156/151‑1 (MQ=255)
                                                                        aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                               <  6:1408566/151‑1 (MQ=255)
                                                                        aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                               <  3:1816647/151‑1 (MQ=255)
                                                                        aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                               <  2:1721640/151‑1 (MQ=255)
                                                                         ccAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGGTATGGGAAGGTGAGCAGATTTtcat                                                                               >  4:1213166/1‑150 (MQ=255)
                                                                           aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCGATATCCGTGAGATGATGAGTGATCGGGCATTAGGCTATGGGTAGGGGGTCAGCGTTTGATcac                                                                            >  3:826685/1‑151 (MQ=255)
                                                                                 ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCACATATCCTTAAGATGATGACTGCTCCGGCATAAGGCTATGGCAAGGTGATCAGATTTTCATCACAcggg                                                                       >  6:1102769/1‑150 (MQ=255)
                                                                                     gCGTTCTGTAAGGCGGTTCAGGATGAAATCCTGATCAGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGTAAtt                                                                   <  5:1592588/150‑1 (MQ=255)
                                                                                     gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCTCCCCCCAATATCCGTCAGATGATGAGTGCTGCTGCATTAGGCTATGGCAAGGGGATCAGATTTTCATAACAGAGGAAtt                                                                   >  8:739895/1‑150 (MQ=255)
                                                                                     gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                  <  1:1014971/151‑1 (MQ=255)
                                                                                      cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATGGTGGATTAGGCTATGGCTAGGTGGTCAGATTTTAATGAGAGGGGAATTat                                                                 >  2:1155690/1‑151 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                           <  8:923823/151‑1 (MQ=255)
                                                                                            gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGt                                                            <  2:795886/150‑1 (MQ=255)
                                                                                              aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                         <  5:226593/151‑1 (MQ=255)
                                                                                                gCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAAt                                                       <  2:593258/151‑1 (MQ=255)
                                                                                                   ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                    <  6:839893/151‑1 (MQ=255)
                                                                                                       cAGGAGGAAATCCTGATCCGGCTATACCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATGCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATgg                                                 <  3:342790/150‑1 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTCAAATGCATGGCa                                               <  1:844369/151‑1 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGATGAGATTTTAATCACAGGGGAATTATGATGTTAAATGCATTGc                                                >  8:1627813/1‑150 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCATTCAGATGATGAGTGAGCCTGCAGTAGGCTATGGCCAGGTGGTCAGATTTTCATAACAGGGGAATTATGATGTTAAATTCATGGc                                                >  3:1474656/1‑150 (MQ=255)
                                                                                                        aggaggAAATCCTGATCCGGCTATTCCGAAAGATATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCa                                               <  2:999770/151‑1 (MQ=255)
                                                                                                         ggaggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTACAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTTAATTCATGGCAc                                              >  4:901035/1‑151 (MQ=255)
                                                                                                           aggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGAAGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCACATTTTAATCACAGGGGAATTATGAGTTTTAATTGATGGCAc                                              >  4:247880/1‑149 (MQ=255)
                                                                                                             gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATACTTCAGATGATGAGTGATGCTGGATTAGGGGATGGCGAGGTGATGAGATTTTCATGACAGGGGATTTATGTTGTTAAATGCATGGCACCTGc                                          >  8:381467/1‑151 (MQ=255)
                                                                                                              aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCGGGCGGGCGCACAGATCGTTCAGCAGGAGAGTCGTCCTGCATTAGGCGAGGGCAAGGGGGGCGGATTTTGCTCACGGGGGGATTTTGTTGTTAGTTGCATGGcgggtggg                                         >  2:1806320/1‑144 (MQ=255)
                                                                                                                aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATAATTCAGATTTTGAGTGATGCTGCATTATGCTATGGCAAGGTGATGAGATTTTAATGAAAGGGGAATTATGTTGTTAAATGCAATGAACCTGCCgg                                       >  5:15116/1‑151 (MQ=255)
                                                                                                                  ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                     <  6:973332/151‑1 (MQ=255)
                                                                                                                  ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAGGGCAAGGGGATCAGATTTTCATCACACGGGAATTATGATGTTAAATTCATGGCCCCTGCCgggg                                     >  7:1365400/1‑149 (MQ=255)
                                                                                                                     gATCCGTCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGc                                    <  4:1845278/149‑1 (MQ=255)
                                                                                                                      aTCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGGCTCCCCCAAATATAATTCAGATGATGACTGAGCCTGCATTATGCTATGGCAAGGTGCTCAGATTTTGATCACAGGGGAATTATGATGTTAAATGAATGGCACCGGCCCGTGcgcc                                 >  1:50053/1‑151 (MQ=255)
                                                                                                                        ccGGCTATTCCGAAAGTTATCCGGCCCCGGCCGCCTCCCCCCCATATCCTACAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCAAAGGGCTATTATGATGTTAAATGAATGGCACCTGCCGGGGgcccc                                >  1:1460109/1‑150 (MQ=255)
                                                                                                                          ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                             <  7:400294/151‑1 (MQ=255)
                                                                                                                            cTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCACATGATGAGTTATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGAATTGAACATGACGGTGCCCCCAtatt                           >  3:395421/1‑148 (MQ=255)
                                                                                                                             tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATACTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATGAATGGCACCTGCCGGTGGCCCCAATTGt                          >  3:1215465/1‑151 (MQ=255)
                                                                                                                                 ccGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCATGCATTATGCTATTGCAAGGTGATCAGATTTTATTCAAAGCGGAATTATTATGTTTAATTAATGGCACCTTCCGCGGCCCCCAATAGTTaa                       >  5:439576/1‑150 (MQ=255)
                                                                                                                                   gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                    <  2:257144/151‑1 (MQ=255)
                                                                                                                                    aaaGTTATCCGCCCCCGGGCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATGACACGGGCATTTTTATGTTTTATGAATGGCGCCTGCCGGGGCCGCCCTTTGTTAAAca                    >  8:112037/1‑150 (MQ=255)
                                                                                                                                     aaGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaa                  <  5:879482/151‑1 (MQ=255)
                                                                                                                                         tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCTGGTGCCCCCATTTGTTAAAcaaagca               <  7:901874/150‑1 (MQ=255)
                                                                                                                                          aTCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAcaaagcaaa             <  6:1865156/151‑1 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa           <  5:960764/151‑1 (MQ=255)
                                                                                                                                            ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa           <  5:1445207/151‑1 (MQ=255)
                                                                                                                                            ccGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATTATGTTAAATGCATGGCACCTGCCGGTGCGCCCATTTGTTAAAcaaagcaa              >  6:550383/1‑148 (MQ=255)
                                                                                                                                            ccGCCCCCCTCCTGCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGCTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGCCCCCATTTGTTTAAcaaagcaa              >  2:535505/1‑148 (MQ=255)
                                                                                                                                             cGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGTCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGCCCCCAATTGTTAAAaaaagaaaaca           >  7:1192871/1‑148 (MQ=255)
                                                                                                                                               cacccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGTAATTATGATGTTAAATGCATGGCACCTGCCGGGGCCCCCATTTGTTAAACAAAGCAAAGATCa        <  1:155141/149‑1 (MQ=255)
                                                                                                                                                ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa        >  7:998173/1‑150 (MQ=255)
                                                                                                                                                 cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAAc      >  1:1746411/1‑151 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  8:235256/151‑1 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  7:510781/151‑1 (MQ=255)
                                                                                                                                                  ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt     <  5:608492/151‑1 (MQ=255)
                                                                                                                                                   ggTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATTTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    <  1:1193208/150‑1 (MQ=255)
                                                                                                                                                   cGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg    >  6:451484/1‑151 (MQ=255)
                                                                                                                                                    gTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGc   >  4:365616/1‑151 (MQ=255)
                                                                                                                                                     tcctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCt  >  6:74839/1‑151 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCT  >  NC_000913/422280‑422579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑