Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913422,4350TG23.7% 788.5 / 62.9 372intergenic (+76/‑83)proY/malZproline‑specific permease/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base T (89/194);  new base G (88/0);  total (178/194)
Fisher's exact test for biased strand distribution p-value = 1.27e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422285‑422585
                                                                                                                                                      |                                                                                                                                                      
  gTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCGCCGtccgccgcc                                                                                                                                                      >  8:1882025/1‑151 (MQ=255)
    ggATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGcccc                                                                                                                                                    >  8:457185/1‑151 (MQ=255)
        tgttgtGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCaa                                                                                                                                                 >  8:1279599/1‑150 (MQ=255)
            gtgCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAACTTATCCGCCCCCGTCCTCCTCCCCCAAATATc                                                                                                                                            <  4:1442502/151‑1 (MQ=255)
              gCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCt                                                                                                                                          <  7:110338/151‑1 (MQ=255)
                 gTTGATTTGCTTGATGTTTAAACGCCGCCACGATCTTCAGCTGGCTTAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                         <  1:152311/149‑1 (MQ=255)
                 gTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCtt                                                                                                                                         <  4:230081/149‑1 (MQ=255)
                  ttGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTc                                                                                                                                        >  8:1769900/1‑149 (MQ=255)
                       tGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCCGCCGCCGCCCCCACAAATCCGTCAgatgat                                                                                                                                 >  2:853789/1‑151 (MQ=255)
                        ggCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAgatgat                                                                                                                                 <  3:34686/150‑1 (MQ=255)
                           tGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCCAATCTCCTTCTGCTGATGCGt                                                                                                                             >  1:33704/1‑151 (MQ=255)
                            ggATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCGCGGTCAACCTGCCCCAAATATCTTTGAGatgatgcttg                                                                                                                            >  3:1789537/1‑147 (MQ=255)
                              aTGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGAt                                                                                                                          <  1:1684858/151‑1 (MQ=255)
                               tGTTTAAACGCCGCCACGATCGTCAGCTGACTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGTCCGGCTCCCCCCAATATGCTTCAGAGGAGGAGTGTTc                                                                                                                         >  1:445919/1‑151 (MQ=255)
                                 tttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCTTCAGTTGATGAGTGATccg                                                                                                                       >  3:590209/1‑150 (MQ=255)
                                  ttAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                      >  6:816999/1‑151 (MQ=255)
                                   tAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCCTTCAGATGATGAGTGTTCCTGc                                                                                                                     >  8:1597545/1‑151 (MQ=255)
                                    aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGc                                                                                                                     <  5:780105/150‑1 (MQ=255)
                                    aaaCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAATATCCGTCAGCTGATGAGTGATGCTgg                                                                                                                     >  5:1338255/1‑149 (MQ=255)
                                      aCGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCAtt                                                                                                                  <  3:1356667/151‑1 (MQ=255)
                                        gccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCGCCCGGCCGCCTCCCCCAAAAATACTTCAGATGATGAGTGATCCTGCCtt                                                                                                                  >  6:1473040/1‑149 (MQ=255)
                                         ccgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAgg                                                                                                               <  6:1829572/151‑1 (MQ=255)
                                          cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGc                                                                                                              <  8:674073/151‑1 (MQ=255)
                                          cgccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATACTTCACATGATGAGTGATCCTGCATTAgg                                                                                                               >  6:570176/1‑150 (MQ=255)
                                           gccACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCCACTATCCTTCAGATGATGAGTGCTCCTGCATTAgggt                                                                                                             >  3:515345/1‑149 (MQ=255)
                                              aCGATCGTCAGCTGGCTGAAAACCATTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCGCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATg                                                                                                          <  7:557090/151‑1 (MQ=255)
                                               cGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATgg                                                                                                         <  4:1003867/151‑1 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                        <  7:350385/151‑1 (MQ=255)
                                                gATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGc                                                                                                        <  4:1711539/151‑1 (MQ=255)
                                                 aTCGGCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCa                                                                                                       <  2:600331/151‑1 (MQ=255)
                                                      cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGGATTAGGCTTTGGGAAGGTg                                                                                                  >  3:748296/1‑151 (MQ=255)
                                                      cAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCACATATCCTTCAGATGATGAGTGATACTGCATTAGGCGATGGCTAGGTg                                                                                                  >  7:807641/1‑151 (MQ=255)
                                                        gctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGGCCTCCGCCCCCACAGATCCTGCAGAGGATGAGTGGTGCTGCGTTAGTCGAGGGCAAgggggt                                                                                                >  1:534558/1‑147 (MQ=255)
                                                         ctggctgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCGCCGCCCCCCAATATCCTGCAGATGATGAGTGATCCTGCATGAGGCTATGGCAAGGTGTTc                                                                                               >  5:1690222/1‑151 (MQ=255)
                                                              tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAtt                                                                                          >  1:1341918/1‑151 (MQ=255)
                                                              tgAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATGCTTCAGATGATGAGTGATCATGGATTATGGTATGGCATGGTGATCAGAtt                                                                                          >  7:1461010/1‑151 (MQ=255)
                                                               gAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAt                                                                                           <  4:1536430/149‑1 (MQ=255)
                                                                aaaaCCAGTACTCCTTGCGTTCTGTCAGCCGGTTCAGGATGATATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGAttt                                                                                         <  4:1276191/150‑1 (MQ=255)
                                                                 aaaCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAgctgttg                                                                                       >  7:1697609/1‑145 (MQ=255)
                                                                 aaaCCAGTAATCCTTGCGTGCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtc                                                                                       <  1:659829/151‑1 (MQ=255)
                                                                   aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                                     <  3:1816647/151‑1 (MQ=255)
                                                                   aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                                     <  5:1368156/151‑1 (MQ=255)
                                                                   aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                                     <  2:1721640/151‑1 (MQ=255)
                                                                   aCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCCCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTtcat                                                                                     <  7:626734/151‑1 (MQ=255)
                                                                      aGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCGATATCCGTGAGATGATGAGTGATCGGGCATTAGGCTATGGGTAGGGGGTCAGCGTTTGATcac                                                                                  >  3:826685/1‑151 (MQ=255)
                                                                       gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATcaca                                                                                 <  1:1746406/151‑1 (MQ=255)
                                                                       gTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCGCCCAAATATGCTTGAGGTGATGAGTGATCCTGCATTTGGCTATGGCAAGGGGATCTGATTTTGATcac                                                                                  >  7:1781165/1‑150 (MQ=255)
                                                                         aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCTCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAg                                                                                <  4:965138/150‑1 (MQ=255)
                                                                         aaTCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAgg                                                                               <  1:1839831/151‑1 (MQ=255)
                                                                          aTCCTTGGGGTGTTTAAGCCCGGTCCTGACGAAATCCTTTTTCGGCTATTCCGAAACTTATTCGGCCCCGGCCTTCTTCCCCAAATATCCTTCAGAAGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAggg                                                                              <  8:1456132/151‑1 (MQ=255)
                                                                            ccTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGa                                                                            <  6:508056/151‑1 (MQ=255)
                                                                                gCGTTCTGTAAGGCGGTTCAGGATGAAATCCTGATCAGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGTAAtt                                                                         <  5:1592588/150‑1 (MQ=255)
                                                                                gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGGCCCCGTCCGCCTCCCCCCAATATCCGTCAGATGATGAGTGCTGCTGCATTAGGCTATGGCAAGGGGATCAGATTTTCATAACAGAGGAAtt                                                                         >  8:739895/1‑150 (MQ=255)
                                                                                gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAAtt                                                                         <  5:1116260/150‑1 (MQ=255)
                                                                                gCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTa                                                                        <  1:1014971/151‑1 (MQ=255)
                                                                                 cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTat                                                                       <  1:850212/151‑1 (MQ=255)
                                                                                 cGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAAAATCCGGCAGATGCTGGGGGCTCCGGCAGTAGGCGAGGGCAAGGGGAGCCGCTGTTGATCACAGGGGCATGat                                                                       >  2:1164403/1‑151 (MQ=255)
                                                                                      tGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCCAATATCCTTCAGAGGATGAGTGGTCCTGCAGTAGGGTTTGGCGAGGTGGTGCGAGTTTGAGCGCAGGGGAGTTATGATGt                                                                  >  7:1540338/1‑151 (MQ=255)
                                                                                       gTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGtt                                                                 <  8:923823/151‑1 (MQ=255)
                                                                                         aaGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTaa                                                               <  5:226593/151‑1 (MQ=255)
                                                                                              ggTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCa                                                          <  6:839893/151‑1 (MQ=255)
                                                                                               ggtCAGGAGGAACTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGGGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAt                                                         <  2:648606/149‑1 (MQ=255)
                                                                                                  cAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCACATATCCTGAAGATGATGAGGGATCGTGGAGTAGGCTATGGCAAGGTGATGAGCTTTTCATGAGAGGGGAATTATGATGTTTAATGCATGGc                                                      >  6:1613179/1‑151 (MQ=255)
                                                                                                   aggaggAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAATATCATTCAGATGATGAGTGAGCCTGCAGTAGGCTATGGCCAGGTGGTCAGATTTTCATAACAGGGGAATTATGATGTTAAATTCATGGc                                                      >  3:1474656/1‑150 (MQ=255)
                                                                                                       ggaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTg                                                 <  5:1442827/151‑1 (MQ=255)
                                                                                                        gaAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCGCGGCCGCCGCCCCCCAATAACCTTCAGATGATGAGTGATGCAGGAGTAGGCGATGGGAAGGGGATGAGATTTTAATAACAGGGGAAGTATGATGTTAGATGCATGGCAGCTGc                                                >  4:357807/1‑151 (MQ=255)
                                                                                                         aaaTCCTGATCCGGCTATTCCGAAAGTTATCCGCGCGCGGCGGGCGGGCGCACAGATCGTTCAGCAGGAGAGTCGTCCTGCATTAGGCGAGGGCAAGGGGGGCGGATTTTGCTCACGGGGGGATTTTGTTGTTAGTTGCATGGcgggtggg                                               >  2:1806320/1‑144 (MQ=255)
                                                                                                           aTCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATAATTCAGATTTTGAGTGATGCTGCATTATGCTATGGCAAGGTGATGAGATTTTAATGAAAGGGGAATTATGTTGTTAAATGCAATGAACCTGCCgg                                             >  5:15116/1‑151 (MQ=255)
                                                                                                             ccTGATCCGGCTATTCCGAAAGTTATCCGCCCGGGGCGGCCGGCCCCAAATCTCCTTCAGATGATGAGTGAGGATGCATTAGGGTATGGGAAGGTGAAGAGATTTTCTTCAAAGGGGAATTATGTTGTTAAATTAATGGAAAGTGCAGGTg                                           >  6:424204/1‑151 (MQ=255)
                                                                                                             ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                           <  6:973332/151‑1 (MQ=255)
                                                                                                             ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTg                                           <  2:1040239/151‑1 (MQ=255)
                                                                                                             ccTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTAGGGCAAGGGGATCAGATTTTCATCACACGGGAATTATGATGTTAAATTCATGGCCCCTGCCgggg                                           >  7:1365400/1‑149 (MQ=255)
                                                                                                                  tCCGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCCAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCGATGGGAAGGGGATCAGCTTTTGATAACAGGGGAATTATGATGTTAAATTCAATGCCCCGGCCGGTGccccc                                      >  1:1391215/1‑151 (MQ=255)
                                                                                                                    cGGCTATTCCGAAAGTTATCCGCCCCCGGCCGCCTCCCCCAAAAATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCCCCTGCCGGGGgccccct                                    >  8:39558/1‑149 (MQ=255)
                                                                                                                     ggCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCAtt                                   <  7:400294/151‑1 (MQ=255)
                                                                                                                        tATTCCGAAAGTTATCCGCCCCCGTCCGCCTCCCCCAAATATCCTTGAGATGATGAGTGGTCCGGCATTAGGGTATGGGAAGGTGATCAGATTTTCATCACAGGGGAATTATGATTTTTATTGCATGGCGCCGGGCGGGGGGCCCATTTGt                                >  8:1137350/1‑151 (MQ=255)
                                                                                                                        tATTCCGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATACTTCAGATGATGAGTGATACTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATTATGTTAAATGAATGGCACCTGCCGGTGGCCCCAATTGt                                >  3:1215465/1‑151 (MQ=255)
                                                                                                                          ttCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGGATTAGGCTATGGCAAGGTGATCAGATTTTGATAACAGGGGAATTATGATTTTAAATTCATTGAACCTGCCGGTGCCCCCATTTGtt                               >  3:1117825/1‑150 (MQ=255)
                                                                                                                            ccGAAAGTTATCCGCCCCCGGCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCATGCATTATGCTATTGCAAGGTGATCAGATTTTATTCAAAGCGGAATTATTATGTTTAATTAATGGCACCTTCCGCGGCCCCCAATAGTTaa                             >  5:439576/1‑150 (MQ=255)
                                                                                                                            ccGAAAGTTATCCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGTCTATGGCAAGGTGATCAGATTTTAATCAAAGGGGAATTATGATGTTAAATTAATGTCAACTGACGGTGCCCCCATTTGTTaa                             >  1:269805/1‑150 (MQ=255)
                                                                                                                            ccGAAAGTTATCCGCCCCCGGCCGCCGCCCCCAAAAATCCTTCAGATGATGAGTGATACTGCATTAGGCTATGGAAAGGTGATCAGATTTTCATCAAACCGGAATTATGATTTTAAATGAATTGCACCTGCCGGTGGCCCCATATGTTaa                             >  5:1842943/1‑150 (MQ=255)
                                                                                                                              gAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAAca                          <  2:257144/151‑1 (MQ=255)
                                                                                                                                aaGTTATCCGCCCCCGGCCTCCGCCCCCAAAAATACTTCAGATGATGAGTGATGCTGCATTAGGCTATGGGAAGGTGATCAGATTTTGATCAAAGGGGAATTATTATGTTTAATGCATGGCACCTGCCGGTGGCGCCAGATGTTAAAcaa                         >  4:1822393/1‑150 (MQ=255)
                                                                                                                                    tATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCTGGTGCCCCCATTTGTTAAAcaaagca                     <  7:901874/150‑1 (MQ=255)
                                                                                                                                    tATCCGCCCCCGGCCTCCTCCCCCAAATATCCGTCAGATGATGAGTGATCCGGCATTAGGCTATGGCCAGGTGATGAGATTTTCATCACAGGGGAATTATGATGTTAAATGCAAGGCACCTGCCGGGGCCCCCAGTTGTTAAAcaaagaa                     >  8:27052/1‑148 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  5:960764/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  6:1348105/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  6:291031/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGa                 <  6:1163891/151‑1 (MQ=255)
                                                                                                                                       ccGCCCCCCTCCTGCTCCCCCAAATATCCTTGAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGCTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGCCCCCATTTGTTTAAcaaagcaa                    >  2:535505/1‑148 (MQ=255)
                                                                                                                                        cGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACaaagaaaaga                 >  3:26562/1‑150 (MQ=255)
                                                                                                                                        cGCCCCCGTCCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGGCTATGTCAAGGTGATCAGATTTTAATAACAGGGGAATTATGATGTTAAATGCATGGAACCTGCCGGTGCCCCCAATTGTTAAAaaaagaaaaca                 >  7:1192871/1‑148 (MQ=255)
                                                                                                                                           ccccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCa              >  7:998173/1‑150 (MQ=255)
                                                                                                                                            cccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCaa             >  2:1610411/1‑150 (MQ=255)
                                                                                                                                             ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           <  5:608492/151‑1 (MQ=255)
                                                                                                                                             ccGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACt           <  7:510781/151‑1 (MQ=255)
                                                                                                                                              ggTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTATGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATTTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTg          <  1:1193208/150‑1 (MQ=255)
                                                                                                                                                 cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc       >  3:1002971/1‑151 (MQ=255)
                                                                                                                                                 cctcctccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTc       <  4:705328/151‑1 (MQ=255)
                                                                                                                                                      tccCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTac  >  4:1142731/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
GCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTAC  >  NC_000913/422285‑422585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑